Method for mineralizing mercury in soil using dissimilatory reduzate of aerobic bacteria

A technology of aerobic bacteria and products, applied in the field of soil remediation, to achieve the effect of large treatment range, low treatment cost and convenient operation

Inactive Publication Date: 2015-09-09
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, amorphous nano-selenium remediation of mercury pollution mainly exists in the atmosphere and water e...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Source and culture of bacteria:

[0024] a. Select the aerobic bacteria as Citrobacter frenchus Citrobacter freundii , inoculate the bacterium liquid into 1 liter of deionized water, 5.0 g of dipotassium hydrogen phosphate, 2.0 g of magnesium chloride, 10 g of yeast extract powder, and 5.0 g of sodium citrate containing 200 ml of culture medium at an inoculum size of 10% by volume. Enrichment culture at a temperature of 30°C for 5 days;

[0025] Preparation of dissimilatory reduction products:

[0026]b. Inoculate the enriched bacterial liquid with a volume ratio of 10% into 200ml medium containing 1 liter of deionized water, 5.0g of dipotassium hydrogen phosphate, 2g of magnesium chloride, 10g of yeast extract powder, citric acid In the Erlenmeyer flask of sodium 5.0g, add sodium selenite 5mmol / L at the same time, adjust the pH value to 7, place the Erlenmeyer flask at 200 rpm, under the aerobic condition of 30°C, after cultivating for 10 days, Ultrasonic cell brea...

Embodiment 2

[0030] Source and culture of bacteria:

[0031] a. Select the aerobic bacteria as Citrobacter frenchus Citrobacter freundii , inoculate the bacterium liquid into 250ml medium containing 1 liter of dipotassium hydrogen phosphate, 1.0g of deionized water, 0.1g of magnesium chloride, 2g of yeast extract powder, and 2.5g of sodium citrate with an inoculation amount of 2% by volume. Enrichment culture at a temperature of 20°C for 3 days;

[0032] Preparation of dissimilatory reduction products:

[0033] b. Inoculate the enriched bacterial liquid with a volume ratio of 2% into 250ml medium containing 1 liter of deionized water, 1.0g of dipotassium hydrogen phosphate, 0.1g of magnesium chloride, 2g of yeast extract powder, lemon Add sodium selenite 1mmol / L to the Erlenmeyer flask containing 2.5g sodium selenite at the same time, adjust the pH value to 11, place the Erlenmeyer flask at 100 rpm, and cultivate it for 5 days under the aerobic condition with a temperature of 20°C , Us...

Embodiment 3

[0037] Source and culture of bacteria:

[0038] a. Select the aerobic bacteria as Citrobacter frenchus Citrobacter freundii , inoculate the bacterium liquid into 1 liter of deionized water, 2.0 g of dipotassium hydrogen phosphate, 1 g of magnesium chloride, 5 g of yeast extract powder, and 3.0 g of sodium citrate containing 300 ml of medium with an inoculum size of 5% by volume. Enrichment culture at 25°C for 4 days;

[0039] Preparation of dissimilatory reduction products:

[0040] b. Inoculate the enriched bacterial solution with a volume ratio of 5% into 300ml medium containing 1 liter of deionized water, 2.0g of dipotassium hydrogen phosphate, 1.0g of magnesium chloride, 5g of yeast extract powder, lemon Add sodium selenite 2mmol / L to the Erlenmeyer flask containing 3.0g of sodium selenite to adjust the pH value to 4, place the Erlenmeyer flask at 150 rpm, and cultivate it for 8 days under aerobic conditions at a temperature of 25°C , Using an ultrasonic cell disruptor...

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PUM

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Abstract

The invention discloses a method for mineralizing mercury in soil using dissimilatory reduzate of aerobic bacteria. The method includes selecting the aerobic bacteria, Citrobacter freundii, which is capable of performing dissimilatory reduction on sodium selenite and is purchased from American Type Culture Collection, under the accession number of ATCC 8090; transferring and culturing the bacteria in a culture medium with the sodium selenite to obtain the dissimilatory reduzate of the aerobic bacteria, and subjecting the mixed bacterial solution containing the dissimilatory reduzate of the aerobic bacteria to crushing in an ultrasonic cell crusher; adding the dissimilatory reduzate of the aerobic bacteria into the soil polluted by the mercury, performing culturing at room temperature for a few days and terminating the experiment. The dissimilatory reduzate of the aerobic bacteria, which uses the sodium selenite as an electron acceptor, is a good mercury trapping agent and is capable of converting the mercury in the soil to residual mercury, so as to mineralize the mercury in the soil and remediate mercury pollution of the soil. The method is applicable to surface mercury polluted soil. The method has the advantages of process simplicity, ease of operation, low cost of processing, wide processing range and no secondary pollution.

Description

technical field [0001] The invention relates to a method for mineralizing soil mercury by using aerobic bacteria dissimilation and reduction products, and belongs to the soil restoration technology in the field of environmental protection. Background technique [0002] Mercury is a highly toxic nonessential heavy metal element that occurs naturally in the Earth's crust. Mercury has three valence states of 0, +1, and +2 under natural conditions, and mainly exists in three forms: elemental mercury (metallic mercury), inorganic mercury (mainly divalent mercury) and organic mercury (mainly methylmercury). . Mercury has a wide range of uses in everyday life. In industry, mercury can be used to extract gold from ore, produce dental filling materials, be used in the chlor-alkali industry, and be used to manufacture thermometers, fluorescent lamps, cosmetics, etc. In agriculture, mercury can also be used as pesticides, fungicides, herbicides, etc. [0003] The large-scale use of...

Claims

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Application Information

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IPC IPC(8): B09C1/10
Inventor 潘响亮王潇男宋文娟
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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