35 results about "Colony number" patented technology

The invention discloses a method for largely expanding endothelial outgrowth cells from peripheral blood. Mononuclear cells separated from the peripheral blood are planted to a pretreated culture dish spread with endothelial outgrowth cells or umbilical endothelial cells as nutritive layers to obtain more endothelial outgrowth cell colonies, and then the endothelial outgrowth cells are largely expanded. By previously spreading the nutritive layer cells containing endothelial cells in the culture dish before planting the mononuclear cells of the peripheral blood, and then planting the mononuclear cells in the culture dish, the method can obtain colony numbers of the endothelial outgrowth cells 20 times more than the traditional method about two weeks in advance, and can obtain the endothelial outgrowth cells with high quality, high quantity and treatment value from the limited peripheral blood.

The invention discloses a freshness preservation method of medicinal materials. The freshness preservation method of the medicinal materials comprises the following steps: sorting harvested fresh medicinal materials, namely shearing and washing the fresh medicinal materials; carrying out sterilization treatment on the washed medicinal materials, and carrying out air-drying; contacting the dried medicinal materials with preservatives, and carrying out air-drying; and packaging the dried medicinal materials by using packaging materials. The freshness preservation method of the medicinal materials utilizes PBAT / PLLA composite ventilate film, so that water desorption of the fresh medicinal materials can be very well prevented, as well as effectively block pollution from the external environment. Being observed in bacteria tests which have been carried out for 1 month, the total colony numbers, the mold colony counts and the Escherichia coli counts of the medicinal materials preserved by using the method disclosed by the invention are all within the requirements of the national standards; thus, the method is capable of very well inhibiting growth of bacteria. Moreover, the preserved medicinal materials are good in appearance characteristics, and kept fresh. Small packages of fresh herbal medicines in small pieces ready for decoction are prepared by using the preserved medicinal materials.

The invention provides a colony selecting throughout improving method, which is applied to a colony selector so as to shorten a selecting path of a probe module and to subsequently improve a selecting throughout; the colony selecting throughout improving method is characterized by comprising the following steps: 1, establishing a mathematical model of the selecting path; 2, sequentially distributing colony numbers and probe numbers, so that an initial chromosome is obtained, and obtaining an initial population in a mode of random insertion; 3, conducting an iterative operation; 4, repeating the step 3 until iteration times reach preset times, so that a final population is obtained; and 5, selecting a chromosome, which has the maximum fitted value, from the final population and outputting the selected chromosome as a result, so that the colony selector can conduct colony selecting in accordance with colony and probe matching in the result. With the application of the colony selecting throughout improving method provided by the invention, the probe module of the colony selector can be optimized, so that the moving path of the probe module is shortened in a selecting course and subsequently the colony selecting throughout is improved.

The invention provides an application of simiarenol in preparation of drugs for treatment of upper respiratory tract infection. Experiments prove that simiarenol has a certain inhibition effect on both respiratory tract infection related viruses and bacteria and also reduces the lung index, the TNF-alpha content, nasal mucosa inflammatory cell number and the nasopharyngeal lavage liquid colony number of mice with upper respiratory tract infection, and indicate that simiarenol has an obvious anti upper respiratory tract infection effect.