35 results about "Colony number" patented technology

The invention relates to a bacterial preparation for degrading petroleum and restoring petroleum polluted soil ecology and a preparation method thereof. The bacterial preparation for degrading the petroleum and restoring the petroleum polluted soil ecology is a composite colony consisting of bacillus megaterium, pseudomonas fluorescens, streptococcus faecalis and candida tropicalis through optimized combination, wherein the matching ratio of the components is 1-2:1:1:0.5-1. The bacterial preparation has the advantage that the bacterial preparation can quickly form a micro-ecological advantage colony after the bacterial preparation is applied to the soil. If 2 to 5 percent of the bacterial preparation is added relative to the weight of surface soil (a cultivation layer of about 15 to 20 centimeters), the oil removal rate in three months is more than or equal to 75 percent (dichloromethane reflux extraction and gravimetric method), and the bacterial preparation has high effective colony number and strong adaptability and has remarkable effect of restoring the petroleum polluted soil ecological environment.

The bacterial colony number sample for verifying food microbiological capacity relates to quality control technology in microbiological detection. The sample is matrix with added Bacillus cereus as leading bacteria and background bacteria. The matrix is practical food, and the background bacteria include one or several kinds of serratia marcescens, Citrobacter freundii, enteroaerogen and escherichia coli. The sample of the present invention has homogeneous colony number content, high finishing rate, and high stability, and may be used in test capacity verification of domestic and international labs. In the same time, the capacity verifying sample may be used in verification of detection method, calibration of test instrument, quality control and check of test result and other aspects.

The invention relates to applications of CAPE (Caffeic Acid Phenylethyl Ester) in culturing hematopoietic stem/progenitor cells in vitro. The CAPE with certain concentration is added in an in-vitro hematopoietic stem/progenitor cell expansion system, the ratio of the expanded human umbilical cord blood mononuclear cells or expanded hematopoietic stem/progenitor cells in CD34 positive cells is obviously increased, the total colony number is remarkably increased, so that the in-vitro expansion of the hematopoietic stem/progenitor cells can be effectively promoted, and the in-vitro expansion efficiency of the hematopoietic stem/progenitor cells can be enhanced.

The invention provides a wheat bacterial count online detection method based on a visible/near infrared spectroscopy technology, and relates to the technical field of detection of the total colony number of wheat. The wheat bacterial count online detection method comprises: collecting wheat samples with different bacterial counts; carrying out spectral detection, scanning the samples in a motion state by using a visible/near infrared optical fiber spectrometer to obtain diffuse reflectance spectrum information, taking part of the samples, and determining the total colony number; pre-processingspectral data, wherein the original spectral information of the samples are pre-processed to eliminate interference factors; carrying out quantitative prediction analysis, and establishing a correlation model between the true bacterial amount and the predicted level according to the corresponding relationship between the total colony number of wheat and the corresponding spectral absorption valueby using a partial least squares regression analysis method (PLSR); and rapidly determining, and outputting the actual bacterial count based on the spectral information of the to-be-detected wheat byusing the established model. According to the present invention, the method has advantages of convenient detection, low detection cost and high detection efficiency, and can perform real-time onlineanalysis on large quantities of samples.

An indoor air purification sterilizing agent comprises the following raw materials in parts by weight: 1 to 5 parts of a lemon-grass extracting solution, 0.05 to 0.1 part of laurate dihydroxyethylamide, 0.5 to 1 part of sodium carboxymethylcellulose and 200 to 250 parts of water. Through the compatibility of the safe and nontoxic lemon-grass extracting solution with laurate dihydroxyethylamide and sodium carboxymethylcellulose, indoor air can be effectively purified, the colony number in the air is reduced, the indoor air quality is improved, the sterilizing rate can reach 99.13%, and besides, the lemon-grass has cool and refreshing fragrance, and can enable the mind and body of a person to be comfortable; side effects on a human body and pollution to environment, which are caused by a conventional chemical disinfection cleaning agent can be effectively avoided, and the indoor air purification sterilizing agent is an environment-friendly and effective indoor air cleaning agent.

The invention discloses a method of predicting shelf life of sauce ducks. Quality indexes of sauce-duck products stored under different temperatures are studied, a kinetic model of sauce-duck qualitychanges is determined through determining, by measurement, laws of changes of total colony numbers of the sauce ducks, escherichia coli, mould, acid values, peroxide values, thiobarbituric acid reactive substances (TBARS) and sensory quality with time, then quality change key-indexes of the mould and the TBARS in a sauce-duck storage period are screened out according to Pearson correlation analysis, and the change laws accord with a zero-order kinetic-model. An Arrhenius equation is combined to establish a shelf-life prediction model of the sauce ducks according to mould and TBARS values of the sauce-duck quality indexes. According to the method, remaining shelf life of sauce ducks in a temperature range of 0-40 DEG C can be quickly and effectively predicted, guidance can be provided for storage, transportation and sales processes to facilitate correspondingly processing sauce-duck products of different freshness in time, and costs can be saved.

The invention discloses a natural sea salt nasal irrigation agent which is prepared through normal-temperature dissolution with purified water and glycerinum as solvent, natural sea salt as solute and domiphen bromide as pathogenic bacteria inhibitor. Every 100 L of nasal irrigation agent contains 0.85-0.95 kg of natural sea salt, 0.5-1.5 L of glycerinum and 0.01-0.05 kg of domiphen bromide. The natural sea salt nasal irrigation agent has the sodium chloride content of 0.85-0.95% (mg/ml), the total heavy metal quantity smaller than or equal to 2 micrograms per milliliter (according to Pb), the pH value of 5.0-7.0 and the cytotoxicity not larger than the second level, causes no sensitization response or vaginal stimulation reaction, has the total bacterial colony number smaller than or equal to 100 cfu/ml and the total fungal colony number smaller than or equal to 100 cfu/ml, can not detect coliform, pseudomonas aeruginosa, staphylococcus aureus and hemolytic streptococcus, can be used for acute and chronic rhinitis, allergic rhinitis, seasonal rhinitis, exercise-induced rhinitis, atrophic rhinitis, nasal polyp, nasal obstruction caused by cold and the like, epistaxis caused by after-nasal-surgery cleaning and dry climatic nasal blood capillary rupture, and the like.

The invention provides an application of simiarenol in preparation of drugs for treatment of upper respiratory tract infection. Experiments prove that simiarenol has a certain inhibition effect on both respiratory tract infection related viruses and bacteria and also reduces the lung index, the TNF-alpha content, nasal mucosa inflammatory cell number and the nasopharyngeal lavage liquid colony number of mice with upper respiratory tract infection, and indicate that simiarenol has an obvious anti upper respiratory tract infection effect.

The invention discloses a method for inspecting cleanliness of asepsis rooms. According to the method, after disinfection of the asepsis room and before and during the sterile experiment, the colony number in air is inspected. The inspecting method is as follows: placing a sampler and a culture dish into the asepsis room to be inspected, culturing the culture medium, and sampling at set time. The invention can effectively check the aseptic environment for medicament production in a simple and effective way, provides the basis for production of high quality medicaments by pharmaceutical enterprises, and can find pollutants in the asepsis room simply, effectively and timely.

The invention relates to a method for maintaining stable contents of Monacolin K and its acid structure in monascus during industrial production. According to the method of the invention, low-temperature drying combined with electron accelerator radiation sterilization is employed during a monascus production process; thus the Monacolin K content and the ratio and contents of the acid structure and the lactone structure in monascus are maintained to be stable; and the control of total colony number in the product at normal temperature is effectively solved. Monascus treated by the method of the invention has a water content of less than 1.0%; the total Monacolin K content in the monascus is 600-1200 mg/100g; the acid structure content of Monacolin K in the monascus accounts for 95-98% of the total Monacolin K content; the lactone structure content of Monacolin K in the monascus accounts for 2-5% of the total Monacolin K content; the total colony number in the monascus is less than 1000 cfu/g; the coliform group is less than 30 MPN/100g; the mould and yeast are less than 100 cfu/g; and no pathogenic bacteria are detected.

The present invention provides a method of screening for a therapeutic or prophylactic drug for acute myeloid leukemia or myelodysplastic syndrome, comprising the following steps:

• • (a) a step of forming colonies of hematopoietic progenitor cells induced from pluripotent stem (iPS) cells produced from non-T cells in blood mononuclear cells isolated from myelodysplastic syndrome patients, in the presence of a test substance and in the absence of the test substance, and
  • (b) a step of selecting the test substance as a candidate for a therapeutic or prophylactic drug for acute myeloid leukemia or myelodysplastic syndrome and the like when the colony number in the presence of the test substance increases from the colony number in the absence of the test substance.

The invention relates to a novel preparation technology of salad dressing in whole-course high-temperature sterilization. The salad dressing is prepared from the following components in parts by weight: 50 parts of salad oil, 11 parts of white granulated sugar, 11 parts of maltitol, 1.2 parts of whey protein, 1.2 parts of edible salt, 25 parts of pure water for drinking, 0.5 part of citric acid, 1part of modified starch, 0.4 part of mustard, 0.1 part of xanthan gum and 0.1 part of glycerel monostearte. The preparation technology comprises the following steps of dissolving, mixing, emulsifying, adjusting acid, uniform stirring, preheating, homogenizing, heating, cooling and packaging. A hot processing technology is adopted, whole-course high-temperature sterilization effects are achieved,the total colony number of the salad dressing made by the new technology is far lower than that by a traditional salad dressing technology, and the preparation technology has the advantages that the sterilization effect is good and the technology is simple.

The invention discloses a chlorine supplementing method for guaranteeing the water quality biological stability of a long-distance water delivery pipe network, and relates to a chlorine supplementing method for guaranteeing the water quality biological stability of the long-distance water delivery pipe network. The invention aims to solve the problems of low residual chlorine concentration and high heterotrophic colony number in water at the tail of the existing urban and rural overall planning long-distance water delivery pipe network. The method comprises the following steps of: mounting a residual chlorine on-line detector and an on-line flow meter on a water conveying pipe network; and calculating residual chlorine values of other points of the water delivery pipe network through a residual chlorine attenuation model, determining an area with the lowest residual chlorine concentration limit value, and determining the quantity and positions of chlorine supplementing points according to the ammonia nitrogen concentration of filtered water and the residual chlorine attenuation model to supplement chlorine. By adopting the chlorine supplementing method, the problems of low concentration of residual chlorine and high heterotrophic colony number in the water at the tail of the existing urban and rural overall planning long-distance water delivery pipe network are solved, the biological stability of the water quality at the tail of the pipe network is effectively guaranteed, and the occurrence of serious peculiar smell in water due to overhigh chlorine input is avoided. The invention belongs to the field of water treatment.

The invention discloses a production method for controlling microbes in dried tofu by combination with a cold chain, and belongs to the technical field of bean product processing. The process for controlling microbe colony numbers mainly comprises the steps of 1) controlling soybean milk microbes, which comprises controlling boiled water addition temperature, incubation temperature and incubation time so as to control the original microbe number; 2) reducing microbe propagation during the production, which comprises rapidly cooling so that total microbial colony number of dried tofu is controlled to a value below 30000cfu; and 3) strictly controlling the circulation time during the dried tofu production. The invention employs the methods for soybean milk original microbe control, cold chain control in the production process, and control in the circulation stage, and dried tofu as a bean product with a total number of colonies less than 100000cfu is obtained.

The invention discloses a biological marker used for liver cancer diagnosis and treatment, and more specifically discloses marker LINC01426. It is found for the first time that differential expressionof LINC01426 is related to generation and development of liver cancer; in patients with liver cancer, expression up-regulation of LINC01426 is detected, it reveal that intervention on LINC01426 geneexpression could be a novel liver cancer treatment method. It is found in verification tests that reduction of LINC01426 expression with inhibitors is capable of reducing propagation and cell formation clone colony number of liver cancer cells obviously.

The invention relates to the technical field of aquatic product preservation, in particular to a fresh-keeping ice coat for freezing aquatic products. The fresh-keeping ice coat is prepared from the raw materials: protamine, calcium chloride, a plant extract, sodium benzoate, chitosan and water, wherein the plant extract is prepared from the components: tea polyphenols, trehalose, ophiopogon japonicus, burdock roots, citron and sea salt. According to the fresh-keeping ice coat for freezing the aquatic products and the application method of the fresh-keeping ice coat, the chitosan and sodium benzoate in the raw materials are used for inhibiting the rapid growth of bacteria in the process of storage of food materials; and natural extract elements of the tea polyphenol, the trehalose, the burdock roots and the like of the plant extract are added, so that the deterioration of the food materials due to protein denaturation is reduced in the process of storage. By using original liquid of the fresh-keeping ice coat, the protein loss rate and decay rate of the aquatic products in the process of preservation can be significantly reduced, the growth of colony number is inhibited, and the fresh-keeping period of the aquatic products in low-temperature storage can be effectively prolonged.

The present invention provides a processing method of plant food additive, comprising the following steps: cleaning the raw material, detecting and adjusting the device, preheating the device, preparing and pre-mixing the supplementary materials, alkalizing, mixing, cooking and crushing the supplementary materials and finally detecting, packaging and producing the finished products. The colony number of the finished products is below 20000 and the mold is in 200 / g or less, fully in accordance with the national food hygiene and safety standards. The processing method of plant food additive has features of scientific and reasonable process, high safety performance, easy operation and monitoring and is suitable for industrialization batch production. The food additive produced by the said processing method is thoroughly sterilized and the water content of the finished product is low and the finished product is easily stored and used with more safety.

The invention discloses a coniella diplodiella prevention and control medicament and belongs to the technical field of plant disease prevention and control. The coniella diplodiella prevention and control medicament is specifically a pesticide composition formed by mixing a chemical pesticide and a biological pesticide according to the mass ratio of (1-3):(1- 3), wherein the chemical pesticide is one of flusilazole emulsifiable concentrate with the concentration of 400 g/L, prochloraz emulsifiable concentrate with the concentration of 250 g/L and propiconazole emulsifiable concentrate with the concentration of 250 g/L; the biological pesticide is one of paenibacillus polymyxa fine granules with the colonies number of 0.1 hundred million/g, 1*106 pythium oligandrum WP and 10% polyoxin B WP. According to the invention, since pesticides used for prevention and control of coniella diplodiella are combined, the effect on coniella diplodiella is improved greatly, unexpected benefits are realized and the yield of grapes can be increased greatly.

A medium composition for increasing the colony number or colony size of microalgae includes, as an effective component, a conditioned medium derived from culture solution during the exponential phase of microalgae in culture. A solid medium contains the medium composition. A method for increasing the colony number or colony size of microalgae includes spreading and culturing microalgae on the solid medium.