Kitchen garbage degrading composite microbial inoculum and preparation method and application thereof

A technology for compounding microorganisms and kitchen waste, applied in the biological field, can solve the problems of long fermentation period, large volume and low nutrient content, and achieve the effects of fast degradation rate, high microbial activity and low dosage

Active Publication Date: 2017-04-26
SHANGHAI NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation cycle is long, the nutrient content is low, and the volume after treatment is large, so it is only suitable for rural areas with inconvenient transportation

Method used

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  • Kitchen garbage degrading composite microbial inoculum and preparation method and application thereof
  • Kitchen garbage degrading composite microbial inoculum and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The preparation method of the kitchen waste composite microbial degradation bacterial agent in this embodiment specifically includes the following steps:

[0060] 1. Preparation of complex microbial culture solution

[0061] (1) The medium for culturing strains of Bacillus amyloliquefaciens, Citrobacter freundii, Methylobacterium radioduranus and Pantoea disperse, the solvent is water, the solute and the concentration are as follows: beef extract 8g / L, peptone 5g / L , sodium chloride 5g / L, dipotassium hydrogen phosphate 5g / L, potassium dihydrogen phosphate 5g / L, magnesium sulfate heptahydrate 1g / L, ammonium sulfate 1g / L, pH 7.2.

[0062] The culture conditions are: temperature 28°C, rotation speed 200r / min, culture time 20h.

[0063] For the culture medium of Pseudomonas oryzae, the solvent is water, and the solute and concentration are as follows: potato 200g / L, glucose 15g / L, pH 7.5. The method of boiling potato juice is as follows: take 200 g of peeled potatoes, cut...

Embodiment 2

[0082] In this example, the kitchen waste composite microbial degradation bacterial agent is a mixed strain composed of Bacillus amyloliquefaciens, Methylobacterium radiodurans, Pantoea disperse, Pseudomonas oryzae, Citrobacter freundii, and Staphylococcus kosheri It is mixed with the carrier, wherein the mixed bacteria account for 15% of the total mass of the degrading bacterial agent, the mass percentage of water in the degrading bacterial agent is 20%, the pH of the degrading bacterial agent is 5.5, and each gram of the degrading bacterial agent contains The number of live bacteria is 10 6 indivual.

[0083] Among them, the mass ratio of Bacillus amyloliquefaciens, Methylobacterium radiodurans, Pantoea disperse, Pseudomonas oryzae, Citrobacter freundii and Staphylococcus koesheri is 1.5:1:1:1:1:1.

[0084] The Bacillus amyloliquefaciens used in this example was preserved in the China Center for Type Culture Collection (CCTCC) at Luojia Mountain (No. 299 Bayi Road), Wuchang...

Embodiment 3

[0109] In this example, the kitchen waste composite microbial degradation bacterial agent is a mixed strain composed of Bacillus amyloliquefaciens, Methylobacterium radiodurans, Pantoea disperse, Pseudomonas oryzae, Citrobacter freundii, and Staphylococcus kosheri It is mixed with a carrier, wherein the mixed bacteria account for 25% of the total mass of the degrading bacterial agent, the mass percentage of water in the degrading bacterial agent is 10%, the pH of the degrading bacterial agent is 8.5, and each gram of the degrading bacterial agent contains The number of live bacteria is 10 9 indivual.

[0110] Among them, the mass ratio of Bacillus amyloliquefaciens, Methylobacterium radiodurans, Pantoea disperse, Pseudomonas oryzae, Citrobacter freundii and Staphylococcus koesei is 3:1.5:1.5:1.5:1.5:1.

[0111] The Bacillus amyloliquefaciens used in this example was preserved in the China Center for Type Culture Collection (CCTCC) at Luojia Mountain (No. 299 Bayi Road), Wucha...

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Abstract

The invention relates to a kitchen garbage degrading composite microbial inoculum and a preparation method and application thereof. The kitchen garbage degrading composite microbial inoculum is prepared by mixing a mixed inoculum of bacillus amyloliquefaciens, radiation-resistant methylobacterium, dispersed pantoea, pseudomonas oryzihabitans, citrobacter freundii and staphylococcus cohnii and a carrier, wherein the mass of the mixed inoculum accounts for 15-25% of the total mass of the kitchen garbage-degrading composite microbial inoculum; the mass ratio of the bacillus amyloliquefaciens, the radiation-resistant methylobacterium, the dispersed pantoea, the pseudomonas oryzihabitans, the citrobacter freundii and the staphylococcus cohnii is (1.5-3):(1-1.5):(1-1.5): (1-1.5):(1-1.5):(1-1.5); the kitchen garbage-degrading composite microbial inoculum is used for degrading kitchen garbage. Compared with the prior art, the kitchen garbage degrading composite microbial inoculum has the advantages as follows: the kitchen garbage degrading composite microbial inoculum can effectively degrade common vegetables, grains, fish, poultry meat and other kitchen garbage, has the degradation rate being above 80%, is high in degradation speed rate and small in odor, does not produce pollutants or poisonous substances, and is low in cost and stable in performance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a kitchen waste composite microbial degradation bacterial agent and a preparation method and application thereof. Background technique [0002] In recent years, my country is in the rapid development of urbanization, and the urban population is growing at a relatively high rate every year. With the gathering of population, the discharge of urban waste is increasing, among which the proportion of kitchen waste is increasing year by year. Since food waste contains a large amount of starch, cellulose, protein, oil and other macromolecular organic substances and water, it provides a good living environment for various microorganisms, which in turn leads to the proliferation of germs, mosquitoes and other harmful organisms. This is extremely harmful to human health and the urban environment. Although kitchen waste has high utilization value, it has high moisture content and low calorific ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/02B09B3/00C12R1/07C12R1/01C12R1/38C12R1/44
CPCB09B3/00C12N1/20C12N11/02
Inventor 李颂硕肖明张瑜张妍
Owner SHANGHAI NORMAL UNIVERSITY
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