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Coliform standard sample in food and preparation method thereof

A technology of standard samples and coliform bacteria, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as foodborne diseases

Inactive Publication Date: 2017-10-03
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the number of coliform bacteria exceeds a certain limit, it increases the risk of pathogenic E. coli and eventually leads to foodborne illness

Method used

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  • Coliform standard sample in food and preparation method thereof
  • Coliform standard sample in food and preparation method thereof
  • Coliform standard sample in food and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Coliform standard samples in food, including target flora and background flora, said target flora consists of Escherichia coli (E.coil), Klebsiella pneumoniae (Klebsiellapnemmoniae), Enterobacter cloacae , Citrobacter freundii (Citrobacterfreundii), the background flora consists of Rhodococcus equi (Rhodococcusequi), Bacillus cereus (Bacillus cereus), Staphylococcus aureus (Staphylococcus aureus).

[0028] The sample is based on trehalose and sterile water, wherein the volume fraction of trehalose is 12%, and the volume fraction of skimmed milk powder is 0.5%.

[0029] The target concentration of the target flora in the sample is 10 3 ~10 4 CFU / mL, the target concentration of background flora is 10 3 ~10 4 CFU / mL.

Embodiment 2

[0031] Such as figure 1 Shown, the preparation method of coliform standard sample in a kind of food in embodiment 1, comprises the selection of sample adding bacterial strain, the preparation of lyoprotectant, sample lyophilization, uniformity and stability test four steps of sample, Specific steps are as follows:

[0032] 1. Selection of sample addition strains

[0033] According to target flora: Escherichia coli (E.coil), Klebsiella pneumoniae (Klebsiellapnemmoniae), Enterobacter cloacae (Enterobacter cloacae), Citrobacter freundii (Citrobacterfreundii), background flora: Bacillus cereus (Bacillus cereus), Staphylococcus aureus (Staphylococcus aureus), and Rhodococcus equi (Rhodococcus equi) were selected as standard strains. All standard strains were purchased from institutions designated by the government, and a strain certificate was attached to ensure the traceability of the strains.

[0034] 2. Preparation of lyoprotectant

[0035] The sample is based on trehalose, s...

Embodiment 3

[0073] Each step of the preparation method of the food microbiology proficiency testing coliform sample described in this example is the same as in Example 2, and the different technical parameters are: in the preparation of the bacterial suspension, each target in the target bacterial suspension The concentration of bacteria is according to 5×10 3 CFU / mL preparation; freeze-drying process 47h.

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Abstract

The invention belongs to the field of quality control in an aspect of microbial detection, and particularly relates to a coliform standard sample in food and a preparation method thereof. The coliform standard sample in food comprises a target flora and a background flora, wherein the target flora consists of colon bacillus, klebsiella pneumoniae, enterobacter cloacae and citrobacter freundii; the background flora consists of rhodococcus equi, bacillus cereus and staphylococcus aureus. The uniformity and the stability conform to the capability verification requirements; the stability of a test sample can be ensured to the maximum degree; even when the content of colon bacillus changes in the transportation and storage process, the total quantity of the coliform cannot be influenced in a sense of statistics. The sample preparation method has the advantages that the process is simple; the success ratio is high.

Description

technical field [0001] The invention belongs to the quality control field of microbiological detection, and in particular relates to a standard sample of coliform bacteria in food and a preparation method thereof. Background technique [0002] The field of food microbiology testing is very special. At present, although some institutions prepare such standard samples, the uniformity and stability of the standard samples can hardly meet the requirements, and transportation is extremely difficult; or the standard samples are pure strains containing only the target bacteria, which is far from the actual sample. It cannot meet the needs of daily laboratory quality control. [0003] Coliform group is not a bacteriological classification name, it does not represent a certain or a certain genus of bacteria, but refers to a group of bacteria related to fecal contamination with certain characteristics, these bacteria are not completely consistent in terms of biochemical characteristi...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12N1/20C12N1/04C12R1/19C12R1/22C12R1/01C12R1/445C12R1/085
CPCC12N1/04C12N1/20C12Q1/10
Inventor 卢行安李珏凌莉吴孝槐赵红阳王伟孙英健王秀君
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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