166 results about "Enterobacteriales" patented technology

The invention discloses an Escherichia coli mutant strain which is classified and named as Escherichia coli, the strain number is CR-TK01, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35554. The invention further discloses a preparation method of the Escherichia coli mutant strain. The Escherichia coli CR-TK01 with high yield of cytidine provided by the invention has the characteristics of high yield and stable high-yield character, glucose is used as a carbon source, the Escherichia coli CR-TK01 is used for fermentation production of cytidine under a certain fermentation condition, and the yield of cytidine is as high as 97.23 g / L; the strain is not degraded after passage for more than 20 generations, the performance of high-yield cytidine is maintained in the passage fermentation process, and the yield of cytidine in passage fermentation is improved. The method for producing cytidine through fermentation is simple in process, high in yield and suitable for industrialization.

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention discloses a lactobacillus mucus fermentation strain. The microbial preservation number of the lactobacillus mucus fermentation strain is CCTCC (China Center for Type Culture Collection) NO: M 20252262. The strain can inhibit enterotoxigenic escherichia coli, and can treat or slow down diarrhea, intestinal oxidative damage, intestinal inflammation damage, intestinal morphological damage, intestinal barrier function damage and intestinal flora disorder caused by the enterotoxigenic escherichia coli. The strain is a probiotic with a clinical application prospect.

The invention discloses a lactobacillus acidophilus FMM01 with the effects of improving the contractility of pelvic floor muscles and repairing mucous membranes and application of the lactobacillus acidophilus FMM01, the lactobacillus acidophilus FMM01 is preserved in the China Center for Type Culture Collection, the preservation date is January 9, 2024, and the preservation number is CCTCC NO: M2024052. The lactobacillus acidophilus FMM01 has the advantages of self-adaption, high acid resistance, strong mucous membrane adhesiveness and no irritation to vaginal mucosa. The lyophilized powder is high in activity and excellent in stability; experimental results show that the lactobacillus acidophilus FMM01 can effectively inhibit bacteria, and especially can effectively inhibit enterobacter cloacae, vibrio parahaemolyticus and / or candida albicans. Besides, the lactobacillus acidophilus FMM01 can improve vaginal relaxation, improve pelvic floor muscle contractility and / or recover vaginal mucosa functions, can promote recovery of healthy florae after mixed vaginitis antibiotic / hormone treatment, improves the treatment effect of mixed vaginitis, and remarkably reduces the recurrence rate of mixed vaginitis.

The present application belongs to the field of microbial technology, and particularly relates to a bacteriophage preparation for controlling foodborne enterobacter and application thereof, wherein the bacteriophage preparation comprises any one or several of bacteriophage PhiEAS1, bacteriophage PhiEHO11, bacteriophage PhiEHO14 and bacteriophage PhiECL22. The four bacteriophages provided by the present application can be used alone or combined into a bacteriophage cocktail, and the influence of the bacteriophage preparation on enterobacter in different ecologies and environments is explored through bacteriophage cocktail therapy. The results show that the bacteriophage preparation exhibits significant bacteriostatic activity on various food substrates contaminated by Enterobacter hormaechei, such as lettuce, pork, milk, cherry tomato and the like; in an animal model, intraperitoneal injection of the bacteriophage preparation can effectively prevent and treat bacteremia caused by Enterobacter hormaechei, and shows potential as an anti-Enterobacter hormaechei biological control agent.

The application belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Mp-GC21, a precursor thereof and application. The amino acid sequence of the antibacterial peptide Mp-GC21 is shown as SEQ ID NO:1, wherein the C terminal is subjected to amidation modification, and two cysteines are connected through a disulfide bond; and the amino acid sequence of the precursor is shown as SEQ ID NO:3. The antibacterial peptide Mp-GC21 is separated from the flower frog, has broad-spectrum antibacterial activity, and has good bacteriostatic and bactericidal effects on standard strains and clinically derived drug-resistant strains of Acinetobacter baumannii, Escherichia coli and Staphylococcus aureus, and can target to destroy the cell membrane of bacteria, remove the biofilm and inhibit the formation of the biofilm. Moreover, the antibacterial peptide Mp-GC21 has good stability, does not have hemolytic activity, cytotoxicity and in-vivo toxicity, and is not easy to induce drug resistance of strains.

The present application belongs to the technical field of artificial intelligence assisted drug screening, and particularly relates to a method for screening active molecules against carbapenem-resistant enterobacteriaceae based on a LightGBM algorithm, a medium and an apparatus. The method constructs a LightGBM integrated learning framework, fuses ADME rules optimized for the outer membrane barrier characteristics of gram-negative bacteria and multi-target molecule docking verification, effectively solves the problem of data imbalance caused by the scarcity of active samples and the huge compound library, and significantly improves the hit rate of screening. The present application also provides a computer readable storage medium and an electronic device. By storing and executing the above program, the present application can quickly and standardizedly identify anti-CRE candidate molecules from a large number of compounds, greatly reducing the computing power cost and time cycle of new drug research and development. The present application is designed to overcome the bottleneck of gram-negative bacterial outer membrane permeation, and provides an efficient, accurate and intelligent screening tool for combating CRE super-bacterial infections.

The invention relates to the technical field of compositions, and particularly discloses a composition for assisting in improving eosinophilic esophagitis and application of the composition. The composition for assisting in improving eosinophilic esophagitis provided by the invention comprises an iron source, a zinc source and L-carnitine. By optimizing the components of the composition and specifically limiting the proportion of the iron source, the zinc source and the L-carnitine in the composition, esophageal epithelial hyperplasia can be effectively reversed, the number of bifidobacteria is increased, the number of enterobacter and esophageal tissue eosinophilic granulocytes is reduced, the level of IL-13 cell factors is reduced, and the curative effect is improved. Further, the effect of remarkably assisting in improving eosinophilic granulocytic esophagitis is achieved.

The invention belongs to the technical field of biological gene engineering, and particularly relates to escherichia coli with high RNA content and a construction method and application thereof.A strong promoter is used for replacing a promoter of escherichia coli rDNA, an expression element of strong promoter + rDNA + terminator is constructed, plasmid is used as a carrier, the expression element is transferred into escherichia coli, and an escherichia coli engineering strain with high RNA yield is constructed; wherein the rDNA is derived from one or more of rrnA, rrnB, rrnC, rrnD, rrnE, rrnG and rrnH of rDNA operons of escherichia coli, and comprises 16S rDNA, 23S rDNA and 5S rDNA in the corresponding operons. By adopting the construction method, the RNA content of the escherichia coli can be obviously improved, and when the method is used for producing RNA, the RNA yield is obviously higher than that of an initial escherichia coli strain and common saccharomycetes for RNA production at present.

The invention discloses a Rogowski eutrophy alkali-producing engineering strain for producing p-coumaric acid as well as a construction method and application of the Rogowski eutrophy alkali-producing engineering strain. The preparation method comprises the following steps: by taking a chemoautotrophic microorganism Ropriavidus alcaligenes H16 as an original strain, firstly, by knocking out a synthetic route of a strain poly-3-hydroxybutyrate (PHB), introducing a tyrosine ammonia lyase gene from Rhodotorula glutinis to construct a basic engineering strain capable of producing p-coumaric acid, and then, carrying out fermentation on the basic engineering strain to obtain the p-coumaric acid. Then, endogenous 3-deoxy-7-phosphoheptanone acid synthase gene aroG1 and chorismate synthase gene aroC are overexpressed, and the total copy is increased, so that the p-coumaric acid synthetic pathway flux of the engineering strain is enhanced; and introducing NAD (P) transhydrogenase gene pntAB from Escherichia coli to improve the supply of cofactors, so as to finally obtain the eutrophy alcaligenes Rosei engineering strain capable of synthesizing p-coumaric acid by using CO2.

The invention relates to a salt and alkali tolerant plant growth promoting enterobacter and application thereof, and belongs to the technical field of microorganism application and agricultural biotechnology, the salt and alkali tolerant plant growth promoting enterobacter is Enterobacter cancerogenic enterobacter PGPR AT1, and the bacterial agent contains the Enterobacter cancerogenic enterobacter PGPR AT1. The growth-promoting enterobacter is applied to plant growth promotion under severe saline-alkali stress conditions or normal growth conditions. The salt-alkali tolerant plant growth promoting enterobacter can directly or indirectly synergistically promote microorganisms for crop growth, and can be prepared into an efficient and stable microbial agent. The microbial agent can achieve a synergistic effect from three aspects of soil, root systems and plant physiology, the physiological stress of crops in the saline-alkali soil is remarkably relieved, the yield, biomass and stress resistance of the crops are fundamentally improved, and long-acting and sustainable improvement on the saline-alkali soil is achieved.

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

This invention provides a polypeptide comprising the sequence shown in any one of SEQ ID NO:1-8. It also provides a composition comprising the polypeptide with the sequence shown in SEQ ID NO:7, cefotaxime, and sulbactam. The polypeptide with the sequence SEQ ID NO:7 exhibits strong inhibitory activity against *Escherichia coli* or *Listeria monocytogenes*. When used in combination with cefotaxime and sulbactam, this polypeptide exhibits strong inhibitory activity against β-lactamase-producing *Escherichia coli*. Therefore, the polypeptide and composition have significant clinical value.

The invention belongs to the technical field of microorganisms, and discloses mulberry endophytic enterobacter H62S42 and application, and the preservation number of the mulberry endophytic enterobacter H62S42 is CCTCC (China Center For Type Culture Collection) M 20252730. The mulberry endophytic enterobacter H62S42 has various characteristics of promoting plant growth and enhancing stress resistance, and can help mulberry to relieve waterlogging stress.

The invention discloses a hirudin escherichia coli engineering bacterium with controllable production generations as well as a construction method and application thereof, and relates to the technical field of biological medicines. The invention discloses a construction method of hirudin escherichia coli engineering bacteria with controllable production generations, which comprises the following steps: constructing a hokC protein gene as shown in the sequence of SEQ ID NO.1 and a constitutive promoter trxA as shown in the sequence of SEQ ID NO.2 into a recombinant expression vector pET28a (+)-ptrxA-hokC, and then transforming the recombinant expression vector into a hirudin synthetic strain E. coli Hirudin HV1-3 to obtain the hirudin escherichia coli engineering bacteria with controllable production generations. Through multiple rounds of culture, the hirudin yield of the engineering strain is reduced to 0 in the tenth round. The method is suitable for various hirudin types, and has the characteristics of reliable operation and high safety.

The invention provides a salt and alkali tolerant phosphate solubilizing bacterium and a growth promoting effect in adversity stress, and belongs to the technical field of biocontrol bacteria. The invention provides a salt and alkali tolerant phosphate solubilizing bacterium, the phosphate solubilizing bacterium comprises enterobacter CCZ3-8, and the phosphate solubilizing bacterium can activate insoluble phosphorus in soil, including inorganic phosphorus and organic phosphorus. The strain CCZ3-8 disclosed by the invention has strong stress resistance, can quickly grow when the NaCl concentration is below 7%, and can obviously slow down the growth rate when the NaCl concentration is greater than 9%; the strain can normally proliferate in a pH range of 4-10, and has relatively strong tolerance to a certain degree of acid and alkali environments at the same time. The phosphate solubilizing bacteria can promote plant growth under a single condition, can promote plant growth in a complex soil environment, can improve the adversity resistance of plants, and has a huge application value.

The invention discloses a specific primer pair for detecting a swine edema disease. The specific primer pair comprises a forward primer Stx2eF and a reverse primer Stx2eR, wherein the nucleotide sequence of the forward primer Stx2eF is as shown in SEQ ID NO: 1, and the nucleotide sequence of the reverse primer Stx2eR is as shown in SEQ ID NO: 2. Data show that the detection result of a sample inoculated with Shiga toxin-producing escherichia coli containing the Stx2e gene by using the primer pair disclosed by the invention is highly consistent with the actual infection degree, and the pathogenic bacterium load can be dynamically quantified through a standard curve. By means of the advantage, early warning of the pig edema disease can be achieved, and a key time window is provided for early prevention and control.

The application discloses a kind of recombinant escherichia coli for producing L-tyrosine and application, belong to genetic engineering and bioengineering technical field.The application knocks out aroP and tyrP gene, expresses the yddG gene of escherichia coli endogenous to modify the aromatic amino acid transport system of escherichia coli, then heterologous expression comes from bifidobacterium fpk and expresses the ppsA and tktA gene of escherichia coli endogenous to increase the precursor supply of shikimic acid pathway, again through the anti feedback gene expression aroG and tyrA 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase, branch acid mutase and prephenate dehydrogenase are removed feedback inhibition, increase the carbon flux of shikimic acid pathway.Knock out tyrR gene removes the repression of TyrR protein to shikimic acid pathway key enzyme, again knock out trpE and pheA gene to block partial side reaction of shikimic acid pathway, so that the synthesis flux of L-tyrosine increases.Finally knock out endogenous poxB gene to realize the modification of acetic acid pathway, realizes the stable fermentation performance of high glucose concentration.

The application discloses a methionine adenosyltransferase mutant and application of the mutant in immobilized reaction, the mutant is obtained by mutating an amino acid sequence of wild-type Escherichia coli methionine adenosyltransferase, the mutant enhances the interaction force between subunits by introducing a disulfide bond at a specific position; and / or, the protein stability is enhanced by constructing a connecting peptide between adjacent subunits. The mutant provided in the application enhances the thermal stability and catalytic efficiency of the enzyme, reduces the optimum reaction pH value, reduces the generation of by-products and the precipitation of a reaction solution. The mutant enzyme can maintain high activity at a temperature of about 40 DEG C, and is particularly suitable for industrial production. In addition, the immobilized form of the mutant enzyme exhibits good stability and reusability in continuous use, significantly reduces production cost, simultaneously reduces environmental pollution, improves the yield of SAM, is more suitable for large-scale industrial production, and has more market competitiveness.

The invention belongs to the technical field of biology, and discloses a polypeptide with broad-spectrum antibacterial activity and a preparation method and application thereof. The antibacterial peptide or the salt thereof is disclosed for the first time, the antibacterial peptide has a good antibacterial effect on common pathogenic bacteria (such as staphylococcus aureus, escherichia coli, salmonella enteritidis and streptococcus suis) and oral pathogenic bacteria (such as fusobacterium nucleatum and porphyromonas gingivalis), and the antibacterial peptide shows slight cytotoxicity on mammalian cells only under high concentration; and the safety is relatively high.

The invention provides simulated water for testing an ultrafiltration water purifier as well as a preparation method and application of the simulated water. The simulated water for testing the ultrafiltration water purifier comprises test water, escherichia coli, humic acid, montmorillonite, aluminum hydroxide and aluminum sulfate. The concentration of montmorillonite in the simulated water for testing the ultrafiltration water purifier is 0.5-2g / L. The preparation method of the simulated water for testing the ultrafiltration water purifier is simple to operate, the bacterial solution, the humic acid stock solution, the montmorillonite stock solution, the aluminum hydroxide stock solution and the aluminum sulfate are mixed with the test water to prepare the simulated water containing colloids, organic matters and bacteria, and the water quality of various tap water can be accurately simulated; the method can be used for evaluating and testing the performance of the ultrafiltration water purifier filter element, field water taking testing is not needed, and the filter element testing cost is reduced.

An antimicrobial peptide having an amino acid sequence selected from SEQ ID NOs: 2-12 is provided. The antimicrobial peptide has antimicrobial activity against Gram-negative bacteria, Campylobacter jejuni, Escherichia coli, Salmonella enterica subsp. enterica serovar typhimurium, Salmonella enterica subsp. enterica serovar choleraesuis, Klebsiella pneumonica, and / or Enterobacter cloacae. A composition, pharmaceutical composition, food additive, cosmetic composition, or hygine product having an antimicrobial peptide including an amino acid sequence selected from SEQ ID NOs: 2-12 is also provided. A method of treating an infectious disease caused by bacteria including a step of administering a pharmaceutical composition having the antimicrobial peptide as an active ingredient is also provided.

The application discloses a preparation method of a rod-shaped NiCo2S4 / NC catalyst based on escherichia coli, which utilizes escherichia coli as a natural nitrogen-containing carbon carrier (NC), and in-situ grows active material NiCo2S4 on the carrier through a hydrothermal method, realizes uniform dispersion of the NiCo2S4, prevents agglomeration of the NiCo2S4, and finally obtains small-size NiCo2S4 nanoparticles uniformly arranged on the natural surface nitrogen-containing carbon carrier (NC) converted by the escherichia coli. The preparation method is simple, the process is controllable, the period is short, the method is green and environment-friendly, and the obtained catalyst has good electrochemical performance.

Fusion proteins that include a backbone protein and at least one heterologous epitope from enterotoxigenic E. coli are provided. In some aspects, the fusion proteins are multiepitope fusion antigen proteins that include an LT B domain. In some aspects, the LT B domain is directly fused to an LT A domain (for example, the LT B domain does not include a starting methionine or signal sequence). In other aspects, the LT B domain includes a starting methionine and signal sequence. Methods of inducing an immune response in pigs utilizing the fusion proteins, such as an immune response that is protective against porcine post-weaning diarrhea, are also provided.

The invention provides anti-enterotoxigenic E. coli (ETEC) adhesin protein antibodies and methods of using the same. One embodiment is an isolated antibody that binds ETEC coli surface antigen 3 (CS3) protein and ETEC coli surface antigen 6 (CS6) protein, wherein the antibody comprises a CS3 / CS6 VHH binding domain comprising the following complementary-determining regions (CDRs): (a) a CDR-H1 comprising the amino acid sequence of GFTFEDYA (SEQ ID NO: 19); (b) a CDR-H2 comprising the amino acid sequence of INMSDGST (SEQ ID NO: 20); and (c) a CDR-H3 comprising the amino acid sequence of AANLTGPSGGGLPGYNYDY (SEQ ID NO: 21). Also disclosed are antibodies comprising said CS3 / CS6 binding VHH and a binding domain capable of binding to an ETEC adhesin protein selected from colonization factor antigen I adhesin subunit E (CfaE), coli surface antigen 1 (CS1), coli surface antigen 4 (CS4), coli surface antigen 14 (CS14), coli surface antigen 17 (CS17), coli surface antigen 19 (CS19), and coli surface antigen 2 (CS2).

The present application relates to the technical field of antibacterial peptides, in particular to IPC C07K7, more particularly to a gingival protection antibacterial peptide or its stereoisomer and a preparation method and application thereof.The present application provides a gingival protection antibacterial peptide or its stereoisomer, which comprises antibacterial peptide MOAMPV14R; the antibacterial peptide MOAMPV14R comprises a structure shown in formula 1.In the present application, a new antibacterial peptide MOAMPV14R is designed and synthesized, which can effectively inhibit the activity of Streptococcus mutans, Porphyromonas gingivalis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Listeria, and M. maltophilia, and the MIC value is 1-8 μg / mL, the antibacterial effect is excellent, the hemolytic activity and cytotoxicity are low, and the safety is good.

The invention provides an enterobacter campanulae W5-2 for recovering rare earth ions and / or degrading ammonia nitrogen and application of the enterobacter campanulae W5-2, and belongs to the technical field of rare earth recovery and treatment. The collection number of the enterobacter campanulae W5-2 is CCTCC (China Center For Type Culture Collection) NO: M 20252359. The enterobacter campanulae W5-2 can be used for recovering rare earth ions and synchronously degrading ammonia nitrogen. The recovery rate of the enterobacter campanulae W5-2 on rare earth ions exceeds 90%, the ammonia nitrogen degradation rate can reach 90% or above, and the treatment process is green and environmentally friendly. The enterobacter campanulata W5-2 provided by the invention can be applied to rare earth tail water treatment, rare earth ions in rare earth tail water can be efficiently recovered, and ammonia nitrogen pollution in the rare earth tail water can be efficiently degraded at the same time. The enterobacter campanulata W5-2 provided by the invention provides a green and environment-friendly microbial method for rare earth tail water treatment, and has a wide application prospect.

This invention belongs to the field of microbial technology, and particularly relates to a screening culture medium for precise intestinal flora transplantation, its preparation method, and a method for enriching Enterobacteriaceae bacteria. The application of the composition provided by this invention in the preparation of intestinal flora screening culture media, through the synergistic effect of the basic nutritional conditions provided by the oligofructose, chlorogenic acid, and CDC anaerobic agar basal medium, enables effective screening and enrichment of Enterobacteriaceae bacteria in fecal samples under in vitro culture conditions, showing excellent application prospects in the isolation, purification, and functional studies of Enterobacteriaceae bacteria.

The invention discloses a microbial inoculum with soil improvement and pollution remediation functions and a preparation method thereof.Enterobacter CJ63 and enterobacter XS81 are obtained through screening and subjected to heavy metal stress domestication, and the microbial inoculum prepared from the two strains achieves synergism of pH adjustment and heavy metal pollution remediation functions and breaks through the limitation of a single function; the preservation rate and the stability of the microbial inoculum are improved, the application range of the microbial inoculum to the pH and the temperature of soil is widened, and the effect of the microbial inoculum in a polluted area is improved. The single microbial agent can meet the comprehensive remediation function, the microbial agent can replace chemical and physical remediation after being applied, green and pollution-free remediation is achieved, the soil heavy metal accumulation risk is reduced, and the requirement for agricultural green development is met.

A method to make Co- or Ni-substituted protoporphyrin IX by incubating an E. coli strain in a rich medium containing iron ions and added Co2+ or Ni2+ ions, for a time and under conditions wherein the E. coli strain produces Co- or Ni-substituted protoporphyrin IX. The E. coli host may be transformed to contain and express a gene construct encoding a mutant EcHemH including a L13R mutation.