297 results about "Serratia species" patented technology

The antimicrobial activity of bovine bactericidal/permeability-increasing protein (bBPI)-derived synthetic peptides against mastitis-causing Gram-negative bacteria was evaluated. Three peptides were synthesized with sequences corresponding to amino acids 65-99 (bBPI_65-99), 142-169 (bBPI_142-169), or the combination of amino acids 90-99 and 148-161 (bBPI_{90-99,148-161}) of bBPI. The bBPI_{90-99,148-161} peptide demonstrated the widest spectrum of antimicrobial activity, with minimum inhibitory (MIC) and bactericidal (MBC) concentration values ranging from 16-64 μg/ml against Escherichia coli, Klebsiella pneumoniae, and Enterobacter spp, and 64-128 μg/ml against Pseudomonas aeruginosa. None of the peptides exhibited any growth inhibitory effect on Serratia marcescens. The antimicrobial activity of bBPI_{90-99,148-161} was inhibited in milk, but preserved in serum. Finally, both bBPI_142-169 and bBPI_{90-99,148-161} were demonstrated to completely neutralize LPS. The peptide bBPI_{90-99,148-161} is a potent neutralizer of the highly pro-inflammatory molecule bacterial LPS and has antimicrobial activity against a variety of Gram-negative bacteria.

The bacterial colony number sample for verifying food microbiological capacity relates to quality control technology in microbiological detection. The sample is matrix with added Bacillus cereus as leading bacteria and background bacteria. The matrix is practical food, and the background bacteria include one or several kinds of serratia marcescens, Citrobacter freundii, enteroaerogen and escherichia coli. The sample of the present invention has homogeneous colony number content, high finishing rate, and high stability, and may be used in test capacity verification of domestic and international labs. In the same time, the capacity verifying sample may be used in verification of detection method, calibration of test instrument, quality control and check of test result and other aspects.

A method and a composition for treatment of pulmonary bacterial infections caused by gram-negative bacteria suitable for treatment of infection caused by Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a concentrated formulation of aztreonam lysinate delivered as an aerosol or dry powder formulation.

The invention relates to the technical field of biology and especially to a recombinant nuclease and a preparation method thereof. The recombinant nuclease is composed of Serratia marcescens extracellular nuclease and a fusion tag peptide fragment. The invention further provides the preparation method for the recombinant nuclease. The preparation method comprises the following steps: optimization of a gene sequence; connection of the coding sequence of the fusion tag peptide fragment and a nuclease gene, cloning and transformation; screening of high-expression bacterial strains and culture; and separation and purification so as to obtain the high purity recombinant nuclease. According to the recombinant nuclease and the preparation method thereof in the invention, an expression scheme is simple, an expression level is high, up to 30%, and a purifying technology has strong specificity and high yield. The recombinant nuclease is applied to technology for medicine products and can be easily removed from products by using affinity chromatographic technology during separation and purification of products, which is beneficial for improvement of product quality.

The invention relates to a method for preparing a pig bone collagen polypeptide chelated calcium supplement. The method comprises the following steps of: 1, preparing pig bone paste, namely crushing, defatting and grinding fresh pig bones to obtain the pig bone paste; 2, preparing bone collagen polypeptide, namely preparing a pig bone paste culture solution, inoculating mixed strains of serratia marcescens and lactobacillus bulgaricus, fermenting, centrifuging, filtering and drying to obtain the bone collagen polypeptide; 3, preparing soluble bone calcium by mixing bone paste residue left after fermentation and hydrochloric acid; and 4, preparing the bone collagen polypeptide chelated calcium supplement, namely chelating the bone collagen polypeptide and the soluble bone calcium to obtainthe bone collagen polypeptide chelated calcium supplement. By the method, the added value of a pig bone resource is improved, the collagen and bone calcium in the pig bones are comprehensively developed and utilized, and the prepared chelated calcium supplement has high solubility in water, is favorable for a human body to absorb and utilize calcium element in the chelate, and can be applied to various fields such as chemical engineering, foods, cosmetics and the like.

The invention relates to serratia marcescens S-JS1 and application of serratia marcescens S-JS1 in pest control. Serratia marcescens S-JS1 solid culture bacteria or bacterium suspension obtained through fermentation culture can both be applied to prevention and treatment of brown planthopper, beet armyworm and prodenia litura. After bacterium suspension of serratia marcescens S-JS1 is sprayed to brown planthopper, the result shows that the correcting death rates of third-instar nymphs, long-wing adult insects and short-wing adult insects of brown planthopper can reach 67.47%, 63.58% and 78.28% within 5 days respectively through the bacterium suspension with concentration of 8*109 cfu/ml; after 100 micro litters of the strain fermentation liquid with concentration of 108 cfu/ml are added to feed blocks (1*1*1 cm), the correcting death rates of third-instar nymphs of beet armyworm and prodenia litura are 45.64% and 32.20% respectively five days after the nymphs eat the feed blocks; the correcting death rates of third-instar nymphs of beet armyworm and prodenia litura are 76.36% and 62.06% respectively 9 days after the nymphs eat the feed blocks.

The invention discloses serratia marcescens NlM280 and an application thereof as an insecticide. A bacterial suspension obtained by the fermentation culturing of the serratia marcescens NlM280 or a supernatant obtained by centrifuging the bacterial suspension can be used for preventing and treating brown planthopper or chilo suppressalis. The results of injecting or feeding the bacterial suspension or the fermented supernatant of the serratia marcescens NlM280 into the brown planthopper show that the fatality rate of the brown planthopper after being injected with the bacterial suspension for 2 days can reach 100%; the fatality rate of the brown planthopper after being fed with the bacterial suspension for 3 days can reach 79.8%; the pathogenic effects of the serratia marcescens NlM280 are not influenced if the supernatant of the fermentation liquor of the serratia marcescens NlM280 is treated at the temperature of below 55 DEG C; the fatality rate of the brown planthopper after being injected with the fermented supernatant for 2 days can reach 97.2%; the effect on the brown planthopper after being fed with the fermented supernatant is hysteretic, i.e., the fatality rate of the brown planthopper after being fed with the fermented supernatant for 6 days reaches 84.2%. After the bacterial suspension or the fermented supernatant is sprayed to rice, if the rice is fed to the chilo suppressalis, the fatality rate of the bacterial suspension or the fermented supernatant to the chilo suppressalis can reach above 80.0% at the 4th day.

The present invention relates to the technical field of microorganism ability verification, and discloses a microorganism ability verification salmonella sample preparation method. According to the method, salmonella is adopted as target bacteria, and Serratia marcescens, Citrobacter freundii and Escherichia coli are adopted as interference bacteria; the method comprises: (A) strain resuscitation and amplification; (B) strain isolation; (C) bacterial suspension preparation; and (D) freeze-drying and packaging; and the prepared samples comprise a simple-level negative sample, a simple-level positive sample, an intermediate-level negative sample, an intermediate-level positive sample, a difficult-level negative sample and a difficult-level positive sample, wherein the sample having the same level are subjected to paired use. According to the present invention, the sample prepared through the method has the good stability, the strain survival rate is high, and the reasonable level standards with different difficulties are provided.

The invention discloses a serratia marcescens strain producing high-purity 2-keto-D-gluconic acid, and belongs to the field of microorganisms. The strain is a serratia marcescens SDSPY-136 strain, is deposited in the China General Microbiological Culture Collection Center in February 9, 2015, and has an accession number of CGMCC No:10548. The strain can adopts glucose and trehalose as carbon sources, cannot ferment arabinos, is free of gas generation during glucose fermentation and is positive to catalase. The strain is used for production of the 2-keto-D-gluconic acid. Production of the 2-keto-D-gluconic acid by the strain is high in glucose conversion ratio and less in by-products, and the obtained 2-keto-D-gluconic acid is high in purity.

The invention discloses Serratia marcescen capable of producing red pigment through fermentation and a method for producing red pigment by fermentation, belonging to the field of fermentation engineering. The method comprises the following steps: carrying out lixiviating, extraction and rotary evaporation so as to obtain a pigment concentrated liquid; separating and purifying through silicagel column chromatography to obtain a pigment component; carrying out UV-Vis (ultraviolet-visible) analysis so as to determine that the maximum absorption peaks are respectively located at the wavelengths of 530nm and 465nm under the condition that the pH value is 3.0 and 9.0; and measuring the accurate relative molecular mass of the pigment component through LC/MS (liquid chromatography-mass spectrometry) analysis so as to determine that the pigment is prodigiosin. In the method provided by the invention, an LB (load balance) liquid cultural medium is adopted for fermentation; and 50 mL of culture solution is contained in a 500mL triangular flask; sterilization is carried out for 15 minutes at the temperature of 115 DEG C; shaking culture is carried out at the temperature of 30 DEG C at the speed of 200r/min, and then centrifuging operation is carried out for 10 minutes at the speed of 8000r/min to remove impurities; and through extraction with acidic methanol, the prodigiosin Serratia marcescen strain pigment is produced and the yield is 150mg/L. Prodigiosin has important application prospect in the fields of tumor treatment, foods, cosmetics and the like.

The invention relates to the field of microorganism, and in particular discloses a serratia marcescens and an application thereof. The serratia marcescens is preserved in the China General Microbiological Culture Collection Center and has the preservation number of CGMCC No.7416. The serratia marcescens is transferred to the 40th generation and still has higher activity in inhibiting rubber powdery mildew and banana vascular wilt bacterial strain spores; the serratia marcescens has higher genetic stability, the rubber powdery mildew prevention effect of the serratia marcescens reaches a chemical prevention effect, and the serratia marcescens has an obvious prevention effect on banana vascular wilt; and moreover, the serratia marcescens cannot pollute the environment, is ecological and safe, and can be widely applied to prevent the rubber powdery mildew and the banana vascular wilt and prepare rubber powdery mildew and banana vascular wilt prevention preparations.

The invention discloses a chitinase ChiCl for deactivating a varroa destructor and application thereof. In the invention, The chitinase ChiCl for deactivating the varroa destructor is prepared by cloning a gene base sequence from a genome DNA of serratia marcescens GEI, and applying a procaryon expression carrier pET-32a(+) to express the gene in colon bacillus. Purification, renaturation and bioassay are carried out on a protein coded by the gene, a result shows that the protein has toxicity to the varroa destructor, and under a condition that the activity of the chitinase ChiCl is 14.7 U/ml, the varroa destructor transfected by the protein has a mortality rate of 25 percent after 5 days. Sequencing analysis shows that the gene is a new sequence of chitinase gene ChiCl types. The invention can be used in an aspect of preventing the mite damage of bees.

The invention belongs to the field of microorganisms and discloses serratia marcescens and a preparation method and application thereof. A sequence of the serratia marcescens is shown as SEQ IN NO:1 in a sequence table. The preparation method includes: extracting a soil sample from the periphery of a waste battery and heavy metal landfill of a solid waste treatment plant, adding into sterile water, vibrating completely, standing to obtain supernatant, diluting the supernatant into different times, respectively spreading onto a culture medium, adding heavy metal ions to the culture medium to carry out culturing, selecting ingle colonies from the culture medium, inoculating to a slant culture medium to obtain original strains; inoculating the original strains to a liquid culture medium, performing constant-temperature shake culturing, standing overnight to obtain a bacterial liquid, diluting the bacterial liquid, respectively putting in sterilization plates, performing ultraviolet radiation, spreading to a solid culture medium, and performing constant-temperature static culturing and enriched heavy metal analysis to obtain the serratia marcescens. The serratia marcescens has an excellent performance in adsorption of Cr2+, Cd2+ and Pb2+ and is an excellent biosorption material.

The invention belongs to the field of examination of microorganisms, and relates to a method for quickly identifying bloodstream infection bacteria by a high-resolution fusion curve method. The method comprises the following steps of: performing smear gram staining on a blood culture positive culture flask to distinguish negative bacteria and positive bacteria; selectively amplifying different 16S fragments for the negative bacteria and the positive bacteria; and analyzing by the high-resolution fusion curve method. Common bloodstream infection pathogenic bacteria comprise staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae, Serratia marcescens, Klebsiella oxytoca, acinetobacter lwoffii, enterobacter aerogenes, stenotrophomonas maltophilia and burkholderia cepacia. By the method, the common bloodstream infection pathogenic bacteria can be measured quickly, efficiently and accurately, so that strong evidences are provided and precious time is striven for subsequent treatment.

The invention provides a serratia marcescens strain and application thereof to production of a ligninolytic enzyme and degradation of lignin and belongs to the technical field of lignin degradation. The serratia marcescens strain SM01 (Serratia marcescens) has a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO. 15175. The serratia marcescens strain providedby the invention is applied to the production of the ligninolytic enzyme. The serratia marcescens strain provided by the invention is applied to the degradation of the lignin. The serratia marcescensstrain has an efficient lignin degradation capability and the accumulated degradation rate of the lignin reaches 92 percent or more.

The invention discloses a biological control serratia marcescens strain for a general tobacco mosaic virus. The code name of the strain is 2A2; the strain is preserved in China General Microbiological Culture Collection Center on 7th Sep. 2011; and the preservation number of the strain is CGMCC No.5230. The strain can inhibit the infection of the general tobacco mosaic virus. Biological-control experiments show that the strain can prevent and control the general tobacco mosaic virus. The invention also provides an antagonist polysaccharide obtained from the strain. The antagonist polysaccharide has very high application value in the prevention and control on the general tobacco mosaic virus.

The invention discloses serratia marcescens as well as a separation method and application thereof. The invention provides a serratia marcescens strain, wherein the strain is the serratia marcescens;the preservation name is serratia marcescens LS-1; the serratia marcescens LS-1 was preserved in China Center For Typical Culture Collection (CCTCC); the preservation address is Wuhan University, LuoJia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province; the preservation date is Nov. 13, 2017; and the preservation number is CCTCC NO: M2017686. The serratia marcescens has pathogenicity on plant pests and has good insecticidal effect when serving as an insecticide.

The invention belongs to the technical field of microorganisms, and particularly relates to serratia marcescens and a separation method and application thereof. The serratia marcescens is preserved in the China center for type culture collection, the preservation number is CCTCC M 2015634, and the preservation date is October 22th, 2015. Naturally dead grubs in fields are subjected to worm surface disinfection and homogenized under aseptic conditions, aquae sterilisata is added, the mixture is smeared to an NA medium plate for culture, a single colony is picked to be purified, and a serratia marcescens strain TC-1 is obtained. The serratia marcescens is used as a biological insecticide, a seed coating material and a fertilizer additive, and the problems of pesticide residues, target organism pesticide resistance, non-point source pollution and the like in agricultural production are reduced.

A biological control bacteria formulation for preventing green house vegetable disease comprises Bacillus circulans, Bacillus pumilus, Bacillus cereus, Bacillus Subtilis, Serratia marcescens, Pseudomonas fluorescens. The preparation method is : mixing the above-mentioned bacteria according to 1:0.4-0.5:0.2-0.3:0.2-0.3:0.2-0.3:0.2-0.3, 200rpm shaking 36 hours in PDA culture liquid in 33 DEG C, 7000rpm centrifuging, preparing formulation using mixed bacteria and bacteria preservative fluid according to 1:40(g/ml), the alive bacteria concentration being 1x10<9>-1x10<14>cfu/ml. The bacteria formulation can be used in dunghill, root irrigation while transplanting after dilution. The preventing effect of bacteria formulation to vegetable disease can reach 75%, yield promoting effect is more than 45%.

The invention relates to the technical field of microorganisms, in particular to a viscose Serratia marcescens strain KH-001. The viscose Serratia marcescens strain KH-001 is preserved in China Center for Type Culture Collection (CCTCC) on September 1st, 2017, and the preservation number of the viscose Serratia marcescens strain KH-001 is CCTCC M2017465. The viscose Serratia marcescens strain KH-001 has strong virulence to Asian citrus psyllid and can be developed into a biocontrol bacterium preparation used for biologically controlling the Asian citrus psyllid. The viscose Serratia marcescens strain KH-001 is efficient, low in toxicity, low in residues, free of pollution, fast in culture, simple to operate, promising in market and application prospect, and the like.