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Method for preparing and purifying prodigiosin

A technology for purifying prodigiosin and prodigiosin, which is applied in the field of preparing and purifying prodigiosin, and can solve problems such as difficulty in purification and preparation of prodigiosin

Inactive Publication Date: 2012-01-11
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for preparing and purifying prodigiosin, which solves the problems in the prior art that prodigiosin is difficult to prepare and difficult to purify after preparation.

Method used

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  • Method for preparing and purifying prodigiosin
  • Method for preparing and purifying prodigiosin
  • Method for preparing and purifying prodigiosin

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Experimental program
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Embodiment

[0060] Preparation and structural identification of the red pigment of the embodiment strain

[0061] Serratia marcesens (Sarratia marcesens) LTH-2 was isolated from Taihu Lake in Wuxi City, Jiangsu Province, the People's Republic of China, and has been preserved in the China Center for Type Culture Collection with the preservation number CCTCC M 2011209.

[0062] The following uses Serratia marcescens LTH-2 as the production strain (the accession number in the Genebank gene sequence database of the National Center for Biotechnology Information (NCBI) in the United States is HM640277), and the method for preparing the red metabolite prodigiosin produced by it is further done detailed instructions.

[0063] Using Serratia marcescens LTH-2 as the production strain, the specific steps for the preparation of high-purity prodigiosin are as follows:

[0064] (1) Preparation of mother seed: culture sourdough yeast in beef extract peptone, inoculate dark red strain LTH-2 on beef extr...

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Abstract

The invention discloses a method for preparing and purifying prodigiosin. According to the method, serratia marcescens is utilized to produce strains; a crude product of prodigiosin is obtained through fermentation culture of mother seeds of serratia marcescens and extraction and condensation of red metabolites; and the crude product of prodigiosin is purified by the methods of silica gel column chromatography and high performance liquid chromatographic separation and subjected to low temperature freeze drying under vacuum so as to obtain a high pure product of prodigiosin. The purity of the high pure product prepared in the invention is greater than 98%; the method for preparing the product is highly effective; organic solvents used in the method can be recovered for cyclic utilization through rotary evaporation.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a method for preparing and purifying prodigiosin. Background technique [0002] Prodigiosin (Prodigiosin), whose structure is shown in formula (I), formal name is 2-methyl-3-pentyl-6-methoxyprodiginine), is a kind of Prodiginin, is produced by Serratia marcescens (Serratia marcescens) marcescens) and a variety of actinomycetes (Streptomyces) and other bacteria synthesized a class of secondary metabolites. Prodigiosin, also known as prodigiosin and prodigiosin, has a three-pyrrole ring skeleton structure, one of which has a methyl group on C2 and a pentyl group on C3. [0003] [0004] Since the discovery of such substances by Amak et al. in 1929, the research on the properties and biological activities of the substances has always attracted the attention of relevant researchers. With the deepening of research, many of its biological activities have been recognize...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/16C07D207/44C12R1/43
Inventor 浦跃朴杨飞尹立红吴巍
Owner SOUTHEAST UNIV
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