94 results about "Prodigiosin" patented technology

The invention discloses Serratia marcescen capable of producing red pigment through fermentation and a method for producing red pigment by fermentation, belonging to the field of fermentation engineering. The method comprises the following steps: carrying out lixiviating, extraction and rotary evaporation so as to obtain a pigment concentrated liquid; separating and purifying through silicagel column chromatography to obtain a pigment component; carrying out UV-Vis (ultraviolet-visible) analysis so as to determine that the maximum absorption peaks are respectively located at the wavelengths of 530nm and 465nm under the condition that the pH value is 3.0 and 9.0; and measuring the accurate relative molecular mass of the pigment component through LC/MS (liquid chromatography-mass spectrometry) analysis so as to determine that the pigment is prodigiosin. In the method provided by the invention, an LB (load balance) liquid cultural medium is adopted for fermentation; and 50 mL of culture solution is contained in a 500mL triangular flask; sterilization is carried out for 15 minutes at the temperature of 115 DEG C; shaking culture is carried out at the temperature of 30 DEG C at the speed of 200r/min, and then centrifuging operation is carried out for 10 minutes at the speed of 8000r/min to remove impurities; and through extraction with acidic methanol, the prodigiosin Serratia marcescen strain pigment is produced and the yield is 150mg/L. Prodigiosin has important application prospect in the fields of tumor treatment, foods, cosmetics and the like.

The invention relates to a method for dyeing wool fabric by using bacterial dye of prodigiosin, which comprises the steps of: (1), dissolving the prodigiosin by using ethyl acetate or N,N-dimethyl formamide, and then adding the dissolved prodigiosin into water to make dyeing solution; and (2), dyeing wool fabric by using the dyeing solution, wherein use amount of the prodigiosin is 0.1-6% of massfraction of the wool fabric; dyeing bath ratio is 1:10 to 1:20; the pH value of the dyeing solution is adjusted to be 4.0-6.0; dyeing temperature is 80-90 DEG C; and dyeing time is 30-90 min. The dyeing method disclosed by the invention is simple; dyed wool fabric has excellent washing fastness and rubbing fastness, is colorful, excellent in antibiotic property, and at least 90% in bacteriostaticrate, and has wide application prospect.

The invention discloses a prodigiosin high-producing strain and a production method of the prodigiosin high-producing strain. The prodigiosin high-producing strain is named as serratiamarcescenszl3 and is stored in China Typical Culture Collection Center with a number of CCTCCNO: M201209 on April 4, 2012 in Wuhan University, Wuhan, China. The prodigiosin high-producing strain disclosed by the invention has the advantages of being fast in growth speed, stable in synthesis of prodigiosin, and high in expression index; after being cultured for 30 to 40 hours in 1 to 5% of a peanut powder culture medium on a 50L fermenting tank at 25 to 30 DEG C, the prodigiosin has a yield up to 6 to 8g/, and purity is more than 98%. With the adoption of a technology of fermenting in batch, fed-batch fermentation technical parameters, an optimized technology of agitating and digesting and a chromatography refine purifying technology provided by the invention, guidance is provided for industrial and scale production of the prodigiosin; and the prodigiosin can be applied to bio-pharmaceuticals and pharmaceutical chemical engineering fields.

The invention discloses a method for dyeing an acrylic fabric with bacterial dye prodigiosin. The method is characterized in that the amount of the dye used is 3 percent (o.w.f); the volume ratio of alcohol solvent to water is 1-2:2-3; the bath ratio is 1 to 40; the pH value is 4.0 to 6.0; the dyeing temperature is 90 to 95 DEG C; and the dyeing time is 50 minutes. The dyed acrylic fabric has high antibacterial property and a bacteriostasis rate of over 90 percent; and in addition, the dyed acrylic fabric has high washing fastness, high rubbing fastness, bright color and great application prospect.

The invention relates to a method for dyeing acrylic fiber fabrics by utilizing bacterium dyestuff prodigiosin, which includes the steps of (1) dissolving the prodigiosin by using ethyl acetate or N, N-dimethylformamide and then adding dissolved prodigiosin into water to produce a dyeing solution, and (2) dyeing the acrylic fiber fabrics by utilizing the dyeing solution, wherein the dosage of theprodigiosin is 0.1-6% of that of acrylic fiber fabrics by mass, dyeing bath ratio is 1:5-20, potential of hydrogen (pH) of an adjusting dye solution is 4.0-6.0, dyeing temperature is 80-90 DEG C, anddyeing time is 30-90 minutes. Compared with a dyeding method of a mixed solution of carbinol or ethanol and the water, dye-uptake rate of the dyestuff is high, level-dyeing property is good, and the dyeing method is simple, easy to operate and low in device requirements. The dyed acrylic fiber fabrics produced by means the method have good washing fastness and abrasion-resisting fastness, bright color, good antibiotic property and wide application prospects, and antibacterial rate is more than 90%.

The invention provides a Serratia marcescens Xd-1 strain with an accession number of CGMCC No. 7734 and a synchronous extraction and fermentation production method for prodigiosin by using the strain. The method is characterized by comprising the following steps: preparing primary seed liquid of the strain; preparing secondary seed liquid of the strain; mixing a liquid fermentation medium with a volume of V and the secondary seed liquid with a volume of 1 to 8% of V in a container and carrying out fermentation for 6 to 18 h under the fermentation conditions of a rotating speed of 130 to 200 r/min, oxygen flux of 0.5 to 1.5 v/(v.m) and fermentation temperature of 25 to 35 DEG C; and adding an extractant with a volume of 8 to 20% of V into the container and continuing fermentation under the fermentation conditions, wherein total fermentation time is 32 to 64 h.

The invention discloses a prodigiosin producing strain, and a production method and applications thereof. The prodigiosin producing strain is Serratia marcescens BWL1001 assigned the accession numberCGMCC No. 18953. The method for producing prodigiosin includes the following steps: preparing a seed solution; transferring the seed solution to a fermentation medium taking soybean oil as a carbon source according to the ratio of 1%(v/v), and performing shake cultivation at a constant temperate of 28 DEG C for 24-28 h to obtain fermentation broth; and performing centrifugation on the fermentationbroth to collect an upper oil phase, adding 1-3 times volume of acid methanol with pH being 3, performing oscillation and uniform mixing, performing centrifugation after standing for 30 min, collecting an acid methanol phase, and obtaining a prodigiosin product after a solvent is removed. The prodigiosin shows good inhibition effects on the growth of microcystis aeruginosa; and the used strain isfast in growing speed, short in fermentation period, high in prodigiosin yield and low in production cost, and has important promotion effects on promoting the industrial application of the prodigiosin.

The invention discloses a serratia marcescens engineering bacterium and application thereof in production of prodigiosin, and belongs to the technical field of biology. The invention provides a serratia marcescens engineering bacterium JNB5-1 delta cpxR capable of realizing high yield of prodigiosin. The serratia marcescens engineering bacterium JNB5-1 delta cpxR is obtained by knocking out a genefor coding response regulatory protein CpxR in serratia marcescens JNB5-1. After the serratia marcescens engineering bacterium JNB5-1 delta cpxR is inoculated into a fermentation culture medium and fermented for 96 hours, the yield of prodigiosin in fermentation liquor can be as high as 5.83 g/L and is improved by 41.9% compared with that of wild serratia marcescens JNB5-1.

The invention discloses a method of producing lipopolysaccharide having antibacterial activity by means of a Serratia marcescens NS-17 bacterial strain. The method includes the steps of activation of the bacterial strain, seed culture, fermenting culture, cell collection, cell wall disruption, and extraction of the lipopolysaccharide. The Serratia marcescens NS-17, which is screened and obtained from soil, can generate a pigment prodigiosin under culture at 30 DEG C, but not generates the prodigiosin under culture at 37 DEG C. In the method, culture temperature is strictly controlled and a simple culture medium is employed; after culture for 32 h, thalluses are collected through centrifugation; polysaccharides are extracted through a phenol-water method after repeated freezing-thawing cell wall disruption; alcohol precipitation is carried out and then the precipitate is washed with ethanol and acetone in gradient concentrations; and vacuum freeze drying is carried out to prepare the lipopolysaccharide having significant antibacterial activity. The prodigiosin polysaccharide has significant inhibition effect on various microorganisms, especially staphylococcus aureus.

The invention provides a preparation method for a carrier-free nano-drug based on natural pigment. The nano-drug is specifically prepared by using a hydrophobic drug of prodigiosin. A purpose of the preparation method is to form carrier-free nano-particles with tumor targeting through self-assembly in water based on the hydrophobic drug of the prodigiosin, so the anti-tumor effect is achieved, andit is more important that many clinical safety problems caused by a nano-carrier are solved.

The invention discloses gene-rcsB-deficient recombinant Serratia marcescens and application thereof and belongs to the technical field of biology. The invention provides a Serratia marcescens engineering strain JNB5-1[delta]rcsB capable of high yielding prodigiosin. The Serratia marcescens engineering strain JNB5-1[delta]rcsB is obtained through knocking out a gene encoding a transcriptional regulation factor RcsB from Serratia marcescens JNB5-1. By inoculating an LB liquid culture medium with the Serratia marcescens engineering strain JNB5-1[delta]rcsB and carrying out fermentation for 24h, the yield of the prodigiosin in fermentation broth can reach up to 116.48mg/L and is 2.28 times that of wild type Serratia marcescens JNB5-1.

The invention discloses the application of a euphausia superba powder in preparation of salmon and bulltrout color enhancing culture feed. The special salmon and bulltrout color enhancing culture feed is prepared through the following components in percent by mass: 10-70% of a euphausia superba powder, 1-20% of soybean protein concentrate, 1-10% of a barley pest powder, 1-10% of a honey bee pupae powder, 1-20% of self-raising flour, 2-10% of a beer yeast powder, 1-10% of starch, 4-20% of fairy shrimp, 2-4% of spirulina, 1-4% of soyabean lecithin, 2-4% of glycine betaine, 1-3% of fish oil, 1-3% of a vitamin mixture, 1-5% of a mineral element mixture, 1-5% of immunopotentiator, 0.5-2% of bacillus subtilis, 0.1-1% of lilac, 0.1-1% of rhodopseudomonas palustris, and 0.1-1% of prodigiosin. The feed shows outstanding attracting performance for salmon and bulltrout; the flesh of salmon and bulltrout can be obviously improved after 10 days and the color is not faded; the flesh of salmon and bulltrout is high in quality and good in mouth feeling; the culture benefit can be obviously increased.

The invention discloses serratia marcescens RZ 21-C6 and application thereof. The preservation number of the serratia marcescens RZ 21-C6 is CGMCCNo.12714. The mutant strain has the advantages that the utilization efficiency of a substrate, the genetic stability and the yield of prodigiosin are high, and the utilization effect of the mutant strain on xylose is particularly good. In a 5L fermentation tank, pesticide residue straw hydrolysate with xylose as a main component is low in price, a biomass carbon source is developed for production of the prodigiosin, the yield of the prodigiosin can reach 15-20 g/L during multi-batch fermentation, and the conversion rate of total reducing sugar directly used for prodigiosin synthesis is 20% or above. The method for producing the prodigiosin through the serratia marcescens at normal pressure and room temperature by means of plasma mutant strain and the utilization of the xylose and the pesticide residue straw hydrolysate by the serratia marcescens can promote achievement of prodigiosin industrialization, and the method can be used for the fields of biological medicines, ecological environmental management, agricultural biological controlling and the like.

The invention discloses an oxymethyltransferase mutant and application thereof in production of prodigiosin, and belongs to the technical field of biology. The invention provides serratia marcescens engineering bacteria JNB5-1/S53CPigF and JNB5-1/G176C/M245C/S53CPigF capable of realizing high yield of prodigiosin. The serratia marcescens engineering bacteria S53CPigF and JNB5-1/G176C/M245C/S53CPigF are used for expressing the S53CPigF and the G176C/M245C/S53CPigF respectively; and the serratia marcescens engineering bacteria JNB5-1/S53CPigF and JNB5-1/G176C/M245C/S53CPigF are respectively inoculated into a fermentation culture medium to be fermented for 96 hours, and the yield of prodigiosin in the fermentation liquor can be respectively up to 7.24 g/L and 7.02 g/L.