25 results about "Prodigiosin" patented technology

Disclosed herein are compositions and methods for using human-derived Janthinobacterium lividum. Compositions improve skin health. Methods may include applying human-derived Janthinobacterium lividum over a host or host area, such as skin or mucosa, to minimize the presence of one or more microbes, maximize therapeutic effects, and / or improve health. A method to minimize a pathogenic microbe may include applying to a surface a composition including human-derived Janthinobacterium lividum and an acceptable carrier. Compositions and methods may include a prebiotic to maximize growth and / or metabolites. Compositions and methods may include human-derived Janthinobacterium lividum metabolites, such as violacein, prodigiosin, indole-3-carboxaldehyde, and lantibiotics, and / or other Postbiotics.

The application discloses application of undecyl-prodigiosin in inhibition of a root rot pathogen (Fusarium oxysporum) of Salvia miltiorrhiza, and belongs to the technical field of microbial pesticides. 25 H 33 The compound has a molecular formula of C 50 N3O, is derived from Streptomyces aurantiacus YS-4, has a concentration-dependent inhibitory effect on the Fusarium oxysporum, an EC of 33.1 μg / mL, and an inhibition rate of 83.3% at 400 μg / mL. The application further provides a preparation containing the compound, wherein the concentration of the compound is not less than 20 μg / mL, and the concentration of the target area after application is controlled in a range of 20 μg / mL to 400 μg / mL, so that the root rot of Salvia miltiorrhiza can be effectively prevented and treated. As a natural microbial metabolite, the compound is environment-friendly, has no residue, and is not easy to produce drug resistance, solves the pollution problem of chemical prevention and treatment, and provides a new scheme for green prevention and treatment of the root rot of Salvia miltiorrhiza.

The invention discloses a gas-producing vibrio strain for producing prodigiosin and a prodigiosin synthetic gene cluster, and belongs to the technical field of microorganisms. The invention relates to a strain B1D10 for producing prodigiosin, the classification name of the strain B1D10 is Vibrio gazogenes, the strain B1D10 is preserved in Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC NO: 66510, the preservation date is June 13, 2025, and the preservation address is No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The strain B1D10 contains a prodigiosin synthetic gene cluster consisting of 16 genes. The strain is rapid in propagation and can be applied to large-scale fermentation production of prodigiosin.

The invention discloses an anti-tumor bacterium medicine based on engineered Serratia marcescens and a preparation method of the anti-tumor bacterium medicine based on engineered Serratia marcescens. According to the anti-tumor bacterium medicine disclosed by the invention, attenuated Serratia marcescens is taken as a carrier, is encapsulated and then is coupled with a targeting peptide, and then is delivered into tumor tissues to play an anti-tumor role; the attenuated Serratia marcescens is subjected to metabolism to generate the prodigiosin, and the attenuated Serratia marcescens is subjected to metabolism to generate the prodigiosin; the anti-tumor bacterium drug is combined with near-infrared laser irradiation to realize an anti-tumor effect. Biosynthesis of a photosensitive natural product prodigiosin is combined with bacterial treatment, and the near infrared (NIR-II) photosensitivity and photothermal effect of the prodigiosin are innovatively utilized, so that the anti-tumor effect is enhanced, precise bacterial removal after treatment is realized, and a feasible solution is provided for clinical application of bacterial treatment.

The application provides an intermediate of a tunicamycin derivative and a synthesis method of the intermediate. The method provided by the application directly protects an amino group of prodigiosin B with phenyl isothiocyanate, then protects a hydroxyl group (a thiourea group is simultaneously subjected to an alkylation reaction), and obtains an intermediate compound with a brand-new structure, then performs a photocatalytic ring-closing reaction, then protects a 5-hydroxyl group, and finally obtains a target compound through a simple Edman degradation reaction. Compared with the prior art, the method has a short reaction route and a significantly improved yield.

The invention provides serratia nematophila, prodigiosin as well as a production method and application of the serratia nematophila and the prodigiosin. The serratia nematophila is serratia nematophila, the strain number is S207, the serratia nematophila is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2025727. The serratia nematophagi can also be a mutant strain which is formed by the serratia nematophagi with the strain number of S207 after the serratia nematophagi is irradiated by ultraviolet rays. The production method of the prodigiosin comprises the following steps: carrying out fermentation culture on the serratia nematophila provided by the invention, and separating the prodigiosin from a fermentation culture product. The serratia nematophila has the characteristic of high yield of the prodigiosin, the prodigiosin produced by using the serratia nematophila has the characteristic of high yield, and the produced prodigiosin has a good application prospect in the aspects of bacteriostasis and preparation of bacteriostatic agents.

The invention discloses application of gentamicin and ceftazidime in enhancing the drug sensitivity of serratia marcescens, and belongs to the field of biological medicine. The serratia marcescens is a strain carrying drug-resistant genes such as OqxB and mdtC, the mass ratio of gentamicin to ceftazidime is (1: 10)-(1: 50), the synergistic effect of gentamicin and ceftazidime is the strongest under the ratio, the sensitivities of serratia marcescens to gentamicin and ceftazidime can be recovered by 12.5 times and 42 times respectively, MBC is synchronously and greatly reduced, and the serratia marcescens has the dual effects of drug sensitivity enhancement and infection treatment; the single dosage is reduced, so that the cost is reduced, and the potential damage of the medicine to a host is reduced while the treatment effect is ensured. The prodigiosin is effective to serratia marcescens carrying various drug-resistant genes such as OqxB, mdtC, AAC (6 ')-Ic, KdpE and CpxA, cost is saved for prevention, control and treatment of serratia marcescens in animal husbandry, and important reference is provided for safe extraction and utilization of prodigiosin and clinical treatment of human serratia marcescens infection.

The invention discloses a method for enhancing the synthesis capacity of a yeast double-metabolic-pathway pigment product and application, and belongs to the field of synthetic biology and metabolic engineering. The method comprises the following steps: constructing a saccharomyces cerevisiae engineering strain capable of simultaneously synthesizing violacein and lycopene; constructing a mutant library of the global transcription factor SPT15; transforming the mutant library into the engineering strain, and screening the mutant strain with increased yield based on bacterial colony color visualization. According to the invention, a single global transcription factor SPT15 is modified, so that the cell metabolism is regulated and controlled on the whole, the synergistic interaction of a diheterologous synthesis pathway is realized, and the tedious multi-gene rational modification is avoided. The method is easy and convenient to operate and short in period, and a new strategy is provided for efficient construction of yeast cell factories for high-yield pigment and other double-way products.

The present application relates to the cross field of aquatic disease prevention and control and natural product antiviral drug research, and in particular to a fish antiviral active drug and application thereof.The present application firstly explicitly defines the antiviral activity of prodigiosin on IHNV and SVCV, expands the application field of prodigiosin, and can provide a new lead compound for the development of IHNV and SVCV virus drugs.

The invention discloses a cycloheptyl prodigiosin culture medium based on natural seawater and a method. The cycloheptyl prodigiosin culture medium comprises the following components: 5g / L to 11g / L of peptone, 1g / L to 1.5 g / L of yeast powder, 1.67 mL / L to 13.33 mL / L of a carbon source, 2g / L to 3g / L of magnesium chloride and a solvent which is the natural seawater. According to the method, the raw materials are low in price, the yield of cycloheptyl prodigiosin can be increased to 2.3 times of the original yield, and the method is suitable for industrial-grade fermentation of Spartinivicinus ruber MCCC1K03745T.

The invention discloses a marine bacterium for producing prodigiosin and a method for producing the prodigiosin, and belongs to the technical field of microorganisms. The invention relates to a marine strain G2-7 for producing prodigiosin, which is classified and named as Zooshikella sp., the strain is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO: 67210, the preservation date is November 4, 2025, and the preservation address is No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The strain Zooshikella sp. G2-7 can be used for efficiently synthesizing the prodigiosin under conventional culture conditions, has the advantages of stable pigment production, easiness in culture and the like, and provides important strain resources for green preparation and synthetic biological modification of the prodigiosin.

The invention discloses a pH-responsive, antibacterial and antioxidant bio-based composite membrane and a preparation method thereof. The composite membrane is formed by sequentially laminating a prodigiosin-bacterial cellulose / polyvinyl alcohol layer (layer I), a bacterial cellulose / polyvinyl alcohol layer (layer II) and a cinnamic acid-bacterial cellulose / polyvinyl alcohol layer (layer III), the layer I provides a pH response indication function, and the layer III provides antibacterial activity and isolates prodigiosin and cinnamic acid through the layer II, so that pH response passivation caused by interaction of the prodigiosin and the cinnamic acid is avoided. The composite membrane is prepared by the following steps: pretreating a bacterial cellulose wet membrane, homogenizing, homogenizing, cleaning, concentrating into homogenate, extracting and doping prodigiosin and cinnamic acid, blending with polyvinyl alcohol, and casting and drying layer by layer. The composite film shows obvious color change in a pH range of 2-12, and has antibacterial activity on staphylococcus aureus and escherichia coli, obvious DPPH / ABTS free radical scavenging performance and good mechanical properties.

The invention discloses a method for promoting synthesis of prodigiosin by utilizing peony seed oil and a fermentation culture medium. A fermentation medium for high-yield synthesis of prodigiosin from serratia marcescens comprises 15.00 g / L of maltose, 13.39 g / L of tryptone, 10 g / L of NaCl and 3.12% (v / v) of peony seed oil. A fermentation medium is inoculated with a RedJ seed culture solution according to the volume ratio of 2%, fermentation culture is conducted for 60 h at the temperature of 20 DEG C and the speed of 200 r / min, and the yield of prodigiosin synthesized by the strain RedJ reaches 180.49 mg / L and is increased by 1.97 times compared with a fermentation medium without peony seed oil. The invention provides a fermentation method for improving the yield of prodigiosin by utilizing the peony seed oil, which not only can be applied to the aspect of large-scale biosynthesis of the prodigiosin, but also finds a new way for high-valued utilization of the peony seed oil.

The invention relates to the technical field of fermentation engineering, and provides a high-yield prodigiosin extract production method, which comprises: S1, preparing a mother strain; s2, preparing a seed solution; s3, primary fermentation culture; s4, carrying out productive multi-stage fermentation culture; s5, extracting a prodigiosin crude extract; and S6, purifying the prodigiosin. In the production process, specific serratia marcescens is selected for activation, optimization and re-fermentation, a multi-stage productive fermentation mode is adopted, and the treated prodigiosin crude extract is purified by using a column chromatography separation technology and a high-pressure liquid phase instrument, so that the content of the obtained prodigiosin is high.

The invention provides a light-sensitive prodigiosin-producing strain and a method for improving the yield of prodigiosin by utilizing light stimulation, the prodigiosin-producing strain is serratia marcescens GEN-01, the preservation number is CCTCC M 2025877, the serratia marcescens GEN-01 is obtained by carrying out multiple rounds of combined mutagenesis treatment on wild serratia marcescens, the preservation number is CCTCC M 2025877, the preservation number is CCTCC M 2025877, the preservation number is CCTCC M 2025877, and the preservation number is CCTCC M 2025877. Compared with the original strain, the prodigiosin production efficiency of the obtained forward mutant strain with high yield of prodigiosin is improved by 6 times. The synthesis of the prodigiosin by the strain is influenced by illumination, the yield of the prodigiosin can be increased by 53.3% and the content is increased by 65.2% when the prodigiosin is fermented and cultured under a red light condition compared with a control group. In a 5L fermentation tank, the yield of the prodigiosin can finally reach 20.5 g / L through continuous feeding. In the fermentation process of the serratia marcescens GEN-01, the pigment production efficiency of the serratia marcescens GEN-01 is remarkably improved by continuously irradiating the serratia marcescens GEN-01 with the red light for 24-72 hours in the secondary metabolism period, and the obtained prodigiosin can be used as a natural antibacterial agent and a pigment additive and is widely applied to the fields of biological medicines, functional foods and ecological agriculture.

The invention discloses a preparation method of a prodigiosin-bacterial cellulose / polyvinyl alcohol composite pH response food preservative film, which comprises the following steps: preparing wet bacterial cellulose by utilizing static fermentation of a Gluconacetobacter hansenii ATCC53582 strain, coarsely crushing, homogenizing for 10 minutes at 2400 rpm, centrifugally cleaning, and concentrating into 8-12 g / L homogenate; the preparation method comprises the following steps: fermenting with Serratia marcescens GEN-01, extracting with acidic methanol, pre-freezing at 40 DEG C, and freeze-drying to obtain prodigiosin powder, preparing a PVA solution with a concentration of 40 g / L, adding PVA accounting for about 40% (w / w) of the dry weight of bacterial cellulose into bacterial cellulose homogenate, adding 0.02-0.04 mmol / L of prodigiosin, stirring at 40 DEG C and 500 rpm for 1 h until full dispersion and uniform mixing, drying at 40 DEG C for 6 h, and balancing at room temperature for 12 h to obtain the composite film. The prepared film has visual response to pH, and is suitable for food freshness indication and fresh-keeping packaging.

The invention relates to the crossing field of aquatic disease prevention and control and natural product antiviral drug research, in particular to a fish antiviral active drug and application thereof. The antiviral activity of the prodigiosin on the IHNV and the SVCV is defined for the first time, the application field of the prodigiosin is expanded, and a new lead compound can be provided for development of drugs for resisting the IHNV and the SVCV.

The application discloses a construction method of a halophilic genetically engineered bacterium with improved bacterioruberin yield, which comprises the following steps: PCR amplification of a gene; enzyme digestion of the PCR product and an expression vector; ligation of the enzyme-digested PCR product and the expression vector; transformation of the ligation product into an E. coli competent cell to obtain a recombinant plasmid; transformation of the recombinant plasmid into a halophilic archaea competent cell; verification and selection of the recombinant halophilic bacterium with successful transformation for induction and culture to make the halophilic bacterium synthesize bacterioruberin. The application constructs a halophilic genetically engineered bacterium expressing lycopene synthetase by using genetic engineering technology. Compared with an initial bacterium, the constructed halophilic genetically engineered bacterium can improve the yield of bacterioruberin, and the yield of bacterioruberin synthesized by the constructed halophilic genetically engineered bacterium is further improved when tryptophan is added for induction. Therefore, the constructed halophilic genetically engineered bacterium can obviously improve the synthesis amount of bacterioruberin, thereby providing an important bacterium for promoting the industrialization application of bacterioruberin.

The invention provides a high-yield prodigiosin method, a prodigiosin protection method and application. The method comprises the following steps: obtaining a serratia nematophagi mutant strain, screening serratia nematophagi with high and stable yield from the mutant strain, carrying out fermentation culture, and separating prodigiosin from a fermentation culture product, the serratia nematophila is serratia nematophila, the strain number of the serratia nematophila is S207, the serratia nematophila is preserved in the China Center for Type Culture Collection, and the preservation number of the serratia nematophila is CCTCC NO: M 2025727. The protection method of the prodigiosin comprises the following steps: storing the prodigiosin in a protective agent with the pH value of 2-6 at 4-60 DEG C, and avoiding the irradiation of ultraviolet light. A large amount of prodigiosin can be rapidly produced by using the method for producing the prodigiosin at high yield, the prodigiosin can be stably preserved by using the protection method for the prodigiosin, the decoloration rate of the prodigiosin can be reduced, and the growth and reproduction of bacteria and fungi can be effectively inhibited by using the bacteriostasis method for bacteriostasis.

This invention discloses a strain of *Serratia marcescens* that produces squalene from soybean residue and its application in composite membrane preparation, belonging to the field of microbial fermentation and biomaterials technology. The technical problem this invention aims to solve is how to produce squalene from soybean residue and modify composite membranes with the product. This invention first protects a strain of *Serratia marcescens*. Serratia marcescens The strain, designated BD 2025, is registered with the China General Microbiological Culture Collection Center (CGMCC) under number CGMCC No. 35196. Using this strain, a yield of 15.9862 mg / g of styraxanthin can be achieved from soybean residue. Furthermore, the styraxanthin-containing modified composite membrane prepared from this product promotes uniform distribution of the membrane matrix and improves the mechanical properties of the composite membrane.

The invention discloses a serratia marcescens anti-tumor fungicide and a preparation method and application thereof, and relates to the field of biological medicine, the serratia marcescens anti-tumor fungicide comprises a recombinant serratia marcescens strain SM-IL12-PDG, the strain integrates a prodigiosin synthetic gene cluster (pigA-N) driven by an anoxic response type promoter (PgldE) and an IL-12 expression unit driven by a low pH response type promoter (PhoP / PhoQ) in an attenuated strain; according to the serratia marcescens anti-tumor fungicide as well as the preparation method and the application thereof, efficient prodigiosin in-situ synthesis and immune factor targeted delivery are synchronously realized under a tumor microenvironment specific activation condition through a synthetic biological gene loop synergistic regulation mechanism; and triple synergistic effects of direct toxin killing, immune response enhancement and tumor matrix remodeling are formed.

The invention belongs to the field of prodigiosin extraction, and discloses a method for directly preparing high-purity and high-activity prodigiosin from a fermentation broth, which comprises the following steps: mixing thallus precipitate separated from the fermentation broth with an acidic alcohol solvent, carrying out ultrasonic treatment, leaching and separation, and concentrating the obtained supernatant to obtain a prodigiosin crude product; performing semi-preparative liquid chromatography separation after filtration and sample loading, performing elution by using a mobile phase, monitoring a chromatographic peak in real time, and collecting fractions of target prodigiosin according to detection signal intensity and / or effluent color change; concentrating and centrifuging to remove supernatant to obtain prodigiosin precipitate, and drying to obtain powdery prodigiosin. According to the method, the extremely simple process of directly preparing the high-purity and high-activity prodigiosin from the fermentation liquor is completed within 1.5 h or less, the prodigiosin with the purity larger than or equal to 99% is directly obtained from the fermentation liquor, the pharmacopoeia-grade purity can be achieved through single equipment, single solvent and single operation, operation is easy and convenient, conditions are stable, and implementation is easy.

The application discloses a method for producing prodigiosin by fermentation of sugarcane molasses and beet molasses. According to the Serratia marcescens PG-Zeno-001 strain developed by the company in the early stage, on the one hand, the by-product of the sugar industry, sugarcane molasses and beet molasses, is used as the carbon source for the production of prodigiosin by optimizing the formula of the fermentation medium; on the other hand, the method for producing prodigiosin by fermentation using the fermentation medium is provided, and in the large-scale fermentation of a 100L fermentation tank, the prodigiosin yield is up to 16.18 g / L at 48 h. The application can greatly reduce the cost of prodigiosin fermentation production, and is expected to build a new closed loop for the environment-friendly production of the sugar industry.

The invention discloses serratia marcescens engineering bacteria with high yield of prodigiosin as well as a construction method and application of the serratia marcescens engineering bacteria. Through a gene engineering technology, a dapB gene of serratia marcescens is knocked out by utilizing a Red homologous recombination system, and a dapB defect type engineering strain is constructed. After the strain is subjected to shake-flask culture in a fermentation culture medium for 60 hours, the yield of prodigiosin reaches 0.91 g / L and is increased by 25.5% compared with that of a wild strain. The construction method of the engineering bacteria is simple and convenient, genetic stability is achieved, and a new way is provided for efficient microbial production of prodigiosin.