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Serratia marcescens NlM280 and application thereof as insecticide

A Serratia marcescens, insecticide technology, applied in the direction of insecticide, application, biocide, etc., can solve the problems of easy drug resistance, poor stability, narrow insecticidal spectrum, etc., and achieve potential application value Effect

Inactive Publication Date: 2014-10-22
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows us to create better crops called Cryptola cryptoolena (the type of plant) which have been shown to reduce insect damage caused by nematodes such as Globodera rostocheli). By culturing these plants under specific conditions, we found they produced certain types of antimicrobial agents like Bacillus thurigrawns subtilispora B2-23, while also showing their ability to kill other harmful organisms from pests living inside them. These properties make it possible to use this material effectively against various diseases associated therewith.

Problems solved by technology

This patented technical problem addressed in this patents relates to controlling various types of agricultural crop pests including root rot mites, leaf hoppers, graft borne destructants, and stubborn molds, particularly those targeting specific genera called Benthionisporanum Boehniax sporadix mealybugles (CMB); while finding novel compounds capable of killing certain strains without producing any negative side effects on their hosts.

Method used

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  • Serratia marcescens NlM280 and application thereof as insecticide
  • Serratia marcescens NlM280 and application thereof as insecticide
  • Serratia marcescens NlM280 and application thereof as insecticide

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Embodiment 1

[0029] Screening, identification and preservation of embodiment 1, bacterial strain N1M280

[0030] (1) Screening of strains

[0031] The brown planthopper natural pathogenic body (body color is all red) of the brown planthopper population Mudgo raised in the Fuyang laboratory of China Rice Research Institute was surface-sterilized with 75% ethanol aqueous solution for 5 minutes, and the body was ground into powder. Bacterial water was serially diluted (the specific dilution gradient is 10 times, 100 times, and 1000 times), and the 1000-fold dilution was taken and spread on the LB solid medium, cultured at 28°C for 12 hours, and a single colony was obtained, and the red single The colony is designated as strain N1M280.

[0032] (2) Identification of strains

[0033] The physiological and biochemical characteristics of the strain are:

[0034] Spread the strain N1M280 on the LB solid medium, activate it at 28°C for 24 hours, see the plate growth of the strain N1M280 figure ...

Embodiment 2

[0040] Embodiment 2, the preparation of bacterial suspension

[0041] (1) Incline cultivation

[0042] Serratia marcescens N1M280 was inoculated onto LB solid medium and kept at 28°C for 24 hours to obtain slants.

[0043] (2) Seed culture: pick a single colony from the slant and inoculate it in 3 mL of LB liquid medium, keep the temperature at 28° C., shake at 200 rpm for 24 hours, and obtain the seed liquid.

[0044] (3) Fermentation culture: Inoculate 500 μL of seed liquid into 50 mL of LB liquid medium for expanded cultivation, keep the temperature at 28°C, shake and cultivate at 200 rpm for 8 hours, and when the OD600 value of the bacteria reaches 1.0, obtain the fermentation culture medium, and use LB Adjust the cell density of the culture medium to l×l0 8 CFU / mL, made into bacterial suspension.

Embodiment 3

[0045] Embodiment 3: the pathogenicity of the bacterial suspension of Serratia marcescens N1M280 to brown planthopper

[0046] (1), the pathogenicity of the strain detected by injection method:

[0047] Select 3-4 instar N. lugens nymphs, and use an eppendorf microinjector (model TransferMan NK2, manufacturer Shanghai Eppende Co., Ltd.), to inject 0.1 μL of the bacterial suspension prepared by the method in Example 2 into each N. lugens planthopper. Where the base is soft, see Figure 4 , with the injection of LB liquid medium as a control, the brown planthopper was inoculated on the rice variety (Taichung Native1) TN1 at the tillering stage after the injection, and each rice plant was covered with a cage, and each cage received 50 brown planthoppers after the injection. Repeat three times. Place the cage in a large basin with water, and control the indoor temperature at 25±1°C and humidity around 80%. The dead insects caused by the injection operation were eliminated withi...

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Abstract

The invention discloses serratia marcescens NlM280 and an application thereof as an insecticide. A bacterial suspension obtained by the fermentation culturing of the serratia marcescens NlM280 or a supernatant obtained by centrifuging the bacterial suspension can be used for preventing and treating brown planthopper or chilo suppressalis. The results of injecting or feeding the bacterial suspension or the fermented supernatant of the serratia marcescens NlM280 into the brown planthopper show that the fatality rate of the brown planthopper after being injected with the bacterial suspension for 2 days can reach 100%; the fatality rate of the brown planthopper after being fed with the bacterial suspension for 3 days can reach 79.8%; the pathogenic effects of the serratia marcescens NlM280 are not influenced if the supernatant of the fermentation liquor of the serratia marcescens NlM280 is treated at the temperature of below 55 DEG C; the fatality rate of the brown planthopper after being injected with the fermented supernatant for 2 days can reach 97.2%; the effect on the brown planthopper after being fed with the fermented supernatant is hysteretic, i.e., the fatality rate of the brown planthopper after being fed with the fermented supernatant for 6 days reaches 84.2%. After the bacterial suspension or the fermented supernatant is sprayed to rice, if the rice is fed to the chilo suppressalis, the fatality rate of the bacterial suspension or the fermented supernatant to the chilo suppressalis can reach above 80.0% at the 4th day.

Description

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Claims

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Application Information

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Owner CHINA NAT RICE RES INST
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