SSR marker BYL8 of brown planthopper resistant genetic locus bph20(t)

A brown planthopper resistance and locus technology, applied in the field of molecular genetics, can solve problems such as changes in resistance stability, and achieve the effects of delaying loss and improving breeding efficiency

Inactive Publication Date: 2011-09-28
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

However, with the emergence of new biotypes of N. lugens, the resistanc

Method used

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  • SSR marker BYL8 of brown planthopper resistant genetic locus bph20(t)
  • SSR marker BYL8 of brown planthopper resistant genetic locus bph20(t)
  • SSR marker BYL8 of brown planthopper resistant genetic locus bph20(t)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Materials and Methods

[0042] 1. Material selection

[0043] After identification of insect resistance, RBPH54 was found to have strong resistance to brown planthopper. Therefore, it was used as a parent and crossed with the susceptible variety TN1, and then backcrossed and selfed after the cross. All generations of plants were identified for insect resistance. (RBPH54 and TN1 were purchased from the Institute of Plant Protection, Guangxi Academy of Agricultural Sciences)

[0044] 2. Identification of insect resistance

[0045] The identification method of brown planthopper resistance refers to the standard seedling plate screening method SSST (IRRI, 1988) used by the International Rice Research Institute, with certain modifications. The soil for raising seedlings is taken from the topsoil of the paddy field. After preliminary treatment, the soil with uniform mud quality is used for raising seedlings. The rice seeds are soaked in water at 25°C for 24 hours, washed and germin...

Embodiment 2

[0080] Example 2 Verification of Molecular Markers

[0081] 1. Materials and methods

[0082] 1.1 Material

[0083] Negative variety: non-resistant gene variety, susceptible variety TN1

[0084] Positive varieties: contain resistance genes, resistant varieties: RBPH54 and ASD7 (purchased from the Institute of Plant Protection, Guangxi Academy of Agricultural Sciences)

[0085] Molecular marker primers: BYL7 and BYL8 and RM222 and RM244 were synthesized by Shanghai Shenggong.

[0086] 1.2 method

[0087] The genomic DNA of rice samples was extracted by CTAB extraction method (the method is the same as that in Example 1). Amplify the sample DNA with primers BYL7 and BYL8 and RM222 and RM244 respectively. The reaction system includes 0.10μM primers, 250μM dNTP, 1×PCR reaction buffer (50mMKCl, 10mM Tris-HCl pH8.3, 1.5mM MgCl 2 ), 100ng DNA template, 1UTaq enzyme. The reaction was carried out in a Tgradient PCR machine. The reaction procedure is: pre-denaturation at 94°C for 5 min, cycling...

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Abstract

The invention provides a molecule marker of brown planthopper resistant gene. According to the present invention, through the hybridization and backcrossing of a resistant parent RBPH54 and a susceptible variety TN1, progenies are obtained. Through respective resistance identifications and molecule genetic linkage analysis of the progeny strains, a brown planthopper resistant major recessive genebph20(t) is obtained. Two nearest molecule markers linked beside bph20(t) are BYL7 and BYL8, which are self-developed SSR markers. The distances from the markers to bph20(t) are 1.3cM and 1.2 cM. With molecule markers linked to the brown planthopper resistant gene, the detection can be carried out to detect whether the pest resistant variety RBPH54 or the derivative varieties (systems) of RBPH54 contain the pest resistant genetic locus. Therefore, the selection of brown planthopper resistant paddy varieties or paddy qualities can be substantially improved in efficiency.

Description

[0001] This application is a divisional application of the patent application whose application date is November 20, 2008, the application number is 200810226943.0, and the invention title is "Major gene for resistance to brown planthopper and its molecular marker method and application". Technical field [0002] The present invention relates to the field of molecular genetics, in particular to the molecular markers of rice brown planthopper resistance major genes, which can be used in the utilization of rice brown planthopper resistance germplasm resources, variety selection and insect resistance identification and so on. technical background [0003] The brown planthopper is one of the most serious pests in rice production areas (Cheng Xianian et al., 2003; Pathak, 1972; Dyck et al., 1979; Sogawa, 1982). Practice has proved that using variety resistance is one of the best ways to prevent brown planthopper damage (Pathak, 1969; Sogawa, 1982). Therefore, discovering and identifyin...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 杨朗李容柏李杨瑞韦素美黄大辉刘驰黄立飞
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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