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Serratia marcescens S-JS1 and application thereof

A technology of Serratia marcescens, S-JS1, applied in the field of microorganisms

Inactive Publication Date: 2016-02-17
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of beet armyworm and Spodoptera litura is still dominated by chemical control, but due to the large amount and high-frequency use of pesticides, Beet armyworm and Spodoptera litura have a high production of various existing pesticides. resistance

Method used

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  • Serratia marcescens S-JS1 and application thereof
  • Serratia marcescens S-JS1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take the 3rd instar nymphs, long-winged and short-winged adults of brown planthopper, put them in a plastic cup containing rice seedlings, and divide them into 8×10 9 The corrected mortality of N. lugens was 67.47%, 63.58% and 78.28% after 5 days of spray treatment with cfu / ml concentration. Mortality as attached figure 1 .

Embodiment 2

[0018] Take the 3rd instar larvae of beet armyworm and Spodoptera litura, and feed them with 100 μl concentration of 10 8 The feed of cfu / ml bacterium liquid, the corrected mortality rate of beet armyworm and Spodoptera litura was respectively 45.64% and 32.20% after 5 days; Corrected mortality rate was respectively 76.36% and 62.06% after 9 days, as attached figure 2 .

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Abstract

The invention relates to serratia marcescens S-JS1 and application of serratia marcescens S-JS1 in pest control. Serratia marcescens S-JS1 solid culture bacteria or bacterium suspension obtained through fermentation culture can both be applied to prevention and treatment of brown planthopper, beet armyworm and prodenia litura. After bacterium suspension of serratia marcescens S-JS1 is sprayed to brown planthopper, the result shows that the correcting death rates of third-instar nymphs, long-wing adult insects and short-wing adult insects of brown planthopper can reach 67.47%, 63.58% and 78.28% within 5 days respectively through the bacterium suspension with concentration of 8*109 cfu / ml; after 100 micro litters of the strain fermentation liquid with concentration of 108 cfu / ml are added to feed blocks (1*1*1 cm), the correcting death rates of third-instar nymphs of beet armyworm and prodenia litura are 45.64% and 32.20% respectively five days after the nymphs eat the feed blocks; the correcting death rates of third-instar nymphs of beet armyworm and prodenia litura are 76.36% and 62.06% respectively 9 days after the nymphs eat the feed blocks.

Description

1. Technical field [0001] The invention relates to the technical field of microorganisms, in particular to Serratia marcescens S-JS1 and its application in pest control. 2. Technical background [0002] Brown planthopper Nilaparvatalugens Belonging to Homoptera and Delphacidae, it is the primary pest of rice in my country and many Asian countries, and poses a serious threat to the safe production of rice. In my country, the rice planting area damaged by brown planthoppers exceeds 4×10 6 hm 2 , in 2005 and 2008 alone, rice losses in China caused by brown planthopper damage amounted to 2.7×10 9 kg. For a long time, chemical insecticides have been the main way to control brown planthoppers, but many adverse effects on the sustainable development of agriculture, such as residues, resistance, re-emergence, damage to natural enemies of pests, and destruction of ecological balance, have also followed, and are increasingly aggravated trend. Therefore, the comprehensive contro...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P7/04C12R1/43
CPCA01N63/00C12N1/20C12N1/205C12R2001/43
Inventor 牛洪涛郭慧芳刘宝生方继朝
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES