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Viscose Serratia marcescens strain

A technology of Serratia marcescens and bacterial strains is applied in the field of microorganisms, which can solve the problems of citrus psyllid control and the like, and achieve the effects of not easily producing drug resistance, strong pathogenicity and simple operation.

Active Publication Date: 2017-12-22
GANNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, the strain has a high control effect on pests such as yellow shank locust and diamondback moth, because the strain can secrete chitinase to destroy the body wall of insects, and secrete an activity that has a killing effect on insects. Substances, although there have been quite a few reports of this kind at home and abroad, but there is no report on the control of citrus psyllids with this fungus.

Method used

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  • Viscose Serratia marcescens strain
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  • Viscose Serratia marcescens strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Isolation and Screening of Entomopathogenic Bacteria

[0020] (1) Separation and purification of bacterial strains:

[0021] From the natural pathogenic body of the citrus psyllid raised in the Pest Laboratory of Gannan Normal University, Ganzhou City, Jiangxi Province, the body color of the citrus psyllid was completely red, and after surface disinfection with 75% ethanol aqueous solution for 5 minutes, it was cleaned with sterile water in the ultra-clean workbench. Rinse with water for 3 times, dry the water on the worm body with sterile filter paper, then place it on LB solid medium, cultivate it in a 28°C incubator for 24 hours, pick the red colony, and carry out isolation and purification culture . A single colony selected for isolation and purification was stored on LB medium and stored in a refrigerator at 4°C.

[0022] LB solid medium preparation method: Add 10g of peptone, 5g of yeast extract powder, and 10g of sodium chloride to 1000mL of distille...

Embodiment 2

[0026] Embodiment 2: the identification of bacterial strain

[0027] (1) Physiological and biochemical identification of strains

[0028] Spread the bacterial strain obtained in Example 1 on the LB solid medium, and cultivate it at a constant temperature of 28°C for 24h. See the plate growth of the bacterial strain figure 1 . The colonies of this strain are basically raised, with an opaque center and irregular edges, with a size of 1-2.5mm, all of which can produce red pigment. The strain was cultured on LB solid medium at 37°C, and the colony of the strain was white.

[0029] (2) Sequence analysis of strains

[0030] Pick a single colony of the bacterial strain obtained in Example 1 and inoculate it in 5ml of LB liquid medium, culture overnight at 28°C, 180rpm for 12h, take fresh bacterial liquid, centrifuge at 12000rpm, 4°C for 10min, and collect the wet cells. According to the extraction method of the TIANamp Bacteria DNAKit Bacterial Genomic DNA Extraction Kit, the abo...

Embodiment 3

[0035] The preparation of embodiment 3 bacterial suspension

[0036] (1) plate culture

[0037] The aforementioned Serratia marcescens strains were diluted and streaked onto LB solid medium, cultured at a constant temperature of 28°C for 24h, and then stored at 4°C.

[0038] (2) Seed liquid culture: Pick a single colony of the above-mentioned Serratia marcescens strain from the plate and inoculate it in 5 ml of LB liquid medium, shake and culture at 28° C. and 180 rpm for 6 hours to obtain a seed liquid.

[0039] (3) Fermentation culture: Take 500 μL of seed solution and inoculate it into 50 ml of LB liquid medium for expansion culture, shake culture at 28° C. and 180 rpm for 12 hours to obtain fermentation culture solution. Centrifuge the fermentation broth at 4°C and 12000 rpm, remove the supernatant, then resuspend with physiological saline, and resuspend twice according to the above steps. The bacterial suspension was serially diluted with 0.8% normal saline, and the dil...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a viscose Serratia marcescens strain KH-001. The viscose Serratia marcescens strain KH-001 is preserved in China Center for Type Culture Collection (CCTCC) on September 1st, 2017, and the preservation number of the viscose Serratia marcescens strain KH-001 is CCTCC M2017465. The viscose Serratia marcescens strain KH-001 has strong virulence to Asian citrus psyllid and can be developed into a biocontrol bacterium preparation used for biologically controlling the Asian citrus psyllid. The viscose Serratia marcescens strain KH-001 is efficient, low in toxicity, low in residues, free of pollution, fast in culture, simple to operate, promising in market and application prospect, and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and relates to a Serratia marcescens strain for preventing and treating citrus psyllids. Background technique [0002] The citrus psyllid is the main insect vector of citrus huanglongbing. Because citrus huanglongbing will lead to the decline of citrus yield and quality, it will seriously threaten the development of citrus growers and even the world's citrus industry. According to reports, more than 50 countries and regions around the world are currently affected by citrus greening disease, and in my country, tens of millions of citrus trees are cut down every year because of citrus greening disease. As the main vector of citrus huanglongbing, the control of insects is extremely urgent. At present, the control of citrus psyllids is still dominated by chemical control, which will not only lead to resistance of citrus psyllids, but also lead to the residue of citrus pesticides, which limits...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P7/04C12R1/425
CPCA01N63/10C12N1/205C12R2001/425
Inventor 胡威张宁邝凡冯祥林林小财谢廉颉钟八莲卢占军
Owner GANNAN NORMAL UNIV
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