Method for preparing human umbilical cord mesenchymal stem cells

A technology for stem cells and human umbilical cord, applied in the field of preparation of human umbilical cord mesenchymal stem cells, can solve the problems of unavoidable, worrying about the effect, and long recovery time, so as to save storage cost, good cell viability and low residue. Effect

Active Publication Date: 2011-02-16
江苏省北科生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional preparation method has the following disadvantages: (1) Cell cryopreservation requires a certain concentration of cells, and the previous cell culture process consumes a lot of manpower and material resources; (2) It takes a long time for cells to fully recover from recovery after recovery. The effect of clinical application in a short period of time is worrisome; (3) cells after resuscitation will inevitably have a small amount of cryopreservation fluid remaining, which cannot be ruled out in a short period of time, which will cause adverse reactions such as nausea, vomiting, and abdominal pain in patients

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  • Method for preparing human umbilical cord mesenchymal stem cells
  • Method for preparing human umbilical cord mesenchymal stem cells
  • Method for preparing human umbilical cord mesenchymal stem cells

Examples

Experimental program
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Effect test

example 1

[0035] (1) Collection of umbilical cord

[0036] Immediately after the full-term fetus was delivered, the umbilical cord was ligated and cut according to the obstetrical routine, the umbilical cord was washed with normal saline, and then disinfected with medical alcohol, and the umbilical cord was placed in the umbilical cord preservation solution at a constant temperature of 2°C. Extract each 4ml of umbilical cord blood as a test sample, and another 4ml of umbilical cord blood from the placenta to collect serum. The composition of the umbilical cord preservation solution is phosphate buffered saline PBS containing penicillin 100U / ml and streptomycin 100U / ml;

[0037] (2) Transportation of the umbilical cord

[0038] Send the umbilical cord blood, maternal blood test samples and the umbilical cord stored in the umbilical cord preservation solution to the handover laboratory;

[0039] (3) Transfer of umbilical cord

[0040] After the registration of the handover laboratory, t...

example 2

[0052] (1) Collection of umbilical cord

[0053] Immediately after the full-term fetus was delivered, the umbilical cord was ligated and cut according to the obstetrical routine, the umbilical cord was rinsed with normal saline, and then disinfected with medical alcohol, the umbilical cord was placed in the umbilical cord preservation solution at a constant temperature of 8°C, and the maternal blood was extracted from the mother and the placenta. Extract each 4ml of umbilical cord blood as a test sample, and extract another 4ml of umbilical cord blood from the placenta to collect serum. The composition of the umbilical cord preservation solution is the culture medium DMEM / F12 containing 20ug / ml gentamicin;

[0054] (2) Transportation of the umbilical cord

[0055] Send the umbilical cord blood, maternal blood test samples and the umbilical cord stored in the umbilical cord preservation solution to the handover laboratory;

[0056] (3) Transfer of umbilical cord

[0057] Afte...

example 3

[0069] (1) Collection of umbilical cord

[0070] Immediately after the full-term fetus was delivered, the umbilical cord was ligated and cut according to the obstetrical routine, the umbilical cord was washed with normal saline, and then disinfected with medical alcohol, the umbilical cord was placed in the umbilical cord preservation solution at a constant temperature of 4°C, and the maternal blood was extracted from the mother and the placenta Extract each 4ml of umbilical cord blood as a test sample, and extract another 4ml of umbilical cord blood from the placenta to collect serum; the composition of the umbilical cord preservation solution is phosphate buffered saline PBS containing penicillin 100U / ml and streptomycin 100U / ml;

[0071] (2) Transportation of the umbilical cord

[0072] Send the umbilical cord blood, maternal blood test samples and the umbilical cord stored in the umbilical cord preservation solution to the handover laboratory;

[0073] (3) Transfer of umb...

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Abstract

The invention relates to a method for preparing human umbilical cord mesenchymal stem cells, which comprises the steps of collection, transportation, handover, separation, freezing, recovering, primary culture and subculture of umbilical cords. The method has simple process, ensures low production cost, good freezing effect, little cell damage, low toxic side effect and no harm to human body and reduces the residual of the freezing protection liquid in the recovered cells of tissue blocks; and the prepared mesenchymal stem cells can be applied to the clinical treatment of various diseases.

Description

technical field [0001] The invention relates to a method for preparing human umbilical cord mesenchymal stem cells. The prepared mesenchymal stem cells are mainly used in stem cell transplantation, tissue engineering, organ transplantation, immunotherapy and the like. Background technique [0002] The cells in human umbilical cord Wharton's Jelly have the characteristics of stem cells such as self-renewal, multi-directional differentiation, and strong proliferation ability. We call them umbilical cord mesenchymal stem cells (huMS℃s). Mesenchymal stem cells are an important member of the stem cell family. They have attracted increasing attention because of their characteristics of supporting hematopoiesis, promoting stem cell implantation, and regulating immunity. They are ideal seed cells for repairing tissue and organ damage caused by aging and disease. [0003] Stem cell culture is a continuous process. Once it leaves the living body and starts primary culture, its various...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 杨兵欧阳溪宋云庆胡祥
Owner 江苏省北科生物科技有限公司
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