Serratia marcescens and preparation method and application thereof

A technology of Serratia marcescens and bacterial liquid is applied in the field of microorganisms, which can solve the problems of poor adsorption capacity of microbial adsorption materials and achieve the effect of good adsorption performance.

Inactive Publication Date: 2016-04-13
THE FIRST AFFILIATED HOSPITAL OF XIAN JIAOTONG UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem of poor adsorption capacity of microbial adsorption materials in the prior ...

Method used

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  • Serratia marcescens and preparation method and application thereof
  • Serratia marcescens and preparation method and application thereof
  • Serratia marcescens and preparation method and application thereof

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Embodiment

[0023] The embodiment of the present invention provides a method for preparing the above-mentioned Serratia marcescens, the method comprising:

[0024] Screening: Extract soil samples at a depth of 15-20cm around the waste batteries and heavy metal landfills in solid waste treatment plants, add 1g of soil samples into 9ml of sterile water and shake fully, and get the supernatant after standing for 5min. The supernatant was diluted and applied to the culture medium respectively, heavy metal ions were added to the culture medium, the culture medium was inverted and cultured in a 28°C incubator for 7 days, and a single colony with a smooth surface was picked from the culture medium and inoculated on the slant On the medium, the original strain of Serratia marcescens was obtained on the slant medium. If there are non-purely cultured strains, use an inoculation loop to pick a little bacterial lawn and streak on the plate until the original purely cultured strains are isolated.

[...

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Abstract

The invention belongs to the field of microorganisms and discloses serratia marcescens and a preparation method and application thereof. A sequence of the serratia marcescens is shown as SEQ IN NO:1 in a sequence table. The preparation method includes: extracting a soil sample from the periphery of a waste battery and heavy metal landfill of a solid waste treatment plant, adding into sterile water, vibrating completely, standing to obtain supernatant, diluting the supernatant into different times, respectively spreading onto a culture medium, adding heavy metal ions to the culture medium to carry out culturing, selecting ingle colonies from the culture medium, inoculating to a slant culture medium to obtain original strains; inoculating the original strains to a liquid culture medium, performing constant-temperature shake culturing, standing overnight to obtain a bacterial liquid, diluting the bacterial liquid, respectively putting in sterilization plates, performing ultraviolet radiation, spreading to a solid culture medium, and performing constant-temperature static culturing and enriched heavy metal analysis to obtain the serratia marcescens. The serratia marcescens has an excellent performance in adsorption of Cr2+, Cd2+ and Pb2+ and is an excellent biosorption material.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Serratia marcescens and its preparation method and application. Background technique [0002] With the rapid development of my country's industry, heavy metal pollution is becoming more and more serious. Heavy metal pollution will reduce the activity of soil microorganisms, seriously reducing soil fertility and quality. Heavy metals will also accumulate in the human body through the food chain, endangering human health. Therefore, the control of heavy metal pollution is imminent. [0003] Traditional treatment methods for heavy metal pollution include: chemical precipitation, ion exchange, activated carbon and silica gel adsorption, electrochemical methods, and membrane separation methods, but the cost of using traditional methods to treat heavy metal ions is relatively high. In recent years, bioremediation technology has been widely used because of its low cost, and achieved sat...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/43
Inventor 杜俊凯代俊张毅周梦舟唐景峰
Owner THE FIRST AFFILIATED HOSPITAL OF XIAN JIAOTONG UNIV
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