Application of serratia marcescens in control of white ants
A technology for Serratia marcescens and termites, which is applied in the biological field, can solve problems such as damage to buildings, difficult to achieve termite eradication by chemical control, adverse effects on human health, etc., and achieve the effect of avoiding impact and avoiding environmental pollution.
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Embodiment 1
[0011] Embodiment 1 Serratia marcescens production preparation technology
[0012] Test material
[0013] Source of Serratia marcescens strains: Collected from termites infected with Serratia marcescens (collected from the campus of Nanjing Forestry University), cultured in the following medium, transplanted once a year, the strain is named as slime sand Reybacillus (Serratia marcescens), and was preserved in the General Microorganism Center of China Microbiological Strain Collection Management Committee on August 15, 2012, and the preservation number is CGMCCNO.6325.
[0014] The termites were collected from the campus of Nanjing Forestry University, and cultured in the dark at 25±1°C after being collected indoors.
[0015] Bacterial Media Formulation
[0016] Bacterial Solid Medium Formula
[0017] Beef extract peptone medium: 3 grams of beef extract, 5 grams of peptone, 5 grams of sodium chloride, 18 grams of agar, 1000 ml of water, pH to 7.2-7.4
[0018] Bacterial...
Embodiment 2
[0024] Example 2: Determination of Serratia marcescens control effect on termites
[0025] Liquid medium dilution:
[0026] Dilute the liquid medium stock solution to 2 -1 ,2 -2 ,2 -3 ,2 -4 and 2 -5 diluted solution.
[0027] Measure OD value:
[0028] The OD value of each concentration solution was measured at a wavelength of 600nm by a spectrophotometer.
[0029] Measure solution concentration
[0030] The solutions of each concentration were diluted to 10 -7 、10 -8 and 10 -9 diluted solution. Use a sterile pipette to absorb 0.1mL of each dilution solution and add them to the culture dish, use a sterile glass spatula to spread the bacterial solution evenly, and place it on the table for 20-30 minutes, so that the bacterial solution penetrates into the surface of the culture medium and then placed upside down in a 28°C incubator for 24 hours, repeated 3 times.
[0031] Count: the number of bacteria per milliliter = the average number of colonies per dilut...
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