Application of serratia marcescens in control of white ants

A technology for Serratia marcescens and termites, which is applied in the biological field, can solve problems such as damage to buildings, difficult to achieve termite eradication by chemical control, adverse effects on human health, etc., and achieve the effect of avoiding impact and avoiding environmental pollution.

Active Publication Date: 2013-03-27
汤方 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because termites are social insects, chemical control is difficult to achieve the effect of eradicating termites
Moreover, a variety of termites are harmful to housing construction and are closely related to the living environment of human beings. Chemical control will have adverse effects on human health

Method used

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  • Application of serratia marcescens in control of white ants
  • Application of serratia marcescens in control of white ants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1 Serratia marcescens production preparation technology

[0012] Test material

[0013] Source of Serratia marcescens strains: Collected from termites infected with Serratia marcescens (collected from the campus of Nanjing Forestry University), cultured in the following medium, transplanted once a year, the strain is named as slime sand Reybacillus (Serratia marcescens), and was preserved in the General Microorganism Center of China Microbiological Strain Collection Management Committee on August 15, 2012, and the preservation number is CGMCCNO.6325.

[0014] The termites were collected from the campus of Nanjing Forestry University, and cultured in the dark at 25±1°C after being collected indoors.

[0015] Bacterial Media Formulation

[0016] Bacterial Solid Medium Formula

[0017] Beef extract peptone medium: 3 grams of beef extract, 5 grams of peptone, 5 grams of sodium chloride, 18 grams of agar, 1000 ml of water, pH to 7.2-7.4

[0018] Bacterial...

Embodiment 2

[0024] Example 2: Determination of Serratia marcescens control effect on termites

[0025] Liquid medium dilution:

[0026] Dilute the liquid medium stock solution to 2 -1 ,2 -2 ,2 -3 ,2 -4 and 2 -5 diluted solution.

[0027] Measure OD value:

[0028] The OD value of each concentration solution was measured at a wavelength of 600nm by a spectrophotometer.

[0029] Measure solution concentration

[0030] The solutions of each concentration were diluted to 10 -7 、10 -8 and 10 -9 diluted solution. Use a sterile pipette to absorb 0.1mL of each dilution solution and add them to the culture dish, use a sterile glass spatula to spread the bacterial solution evenly, and place it on the table for 20-30 minutes, so that the bacterial solution penetrates into the surface of the culture medium and then placed upside down in a 28°C incubator for 24 hours, repeated 3 times.

[0031] Count: the number of bacteria per milliliter = the average number of colonies per dilut...

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Abstract

The invention discloses application of serratia marcescens in control of white ants and a key preparation technology. The serratia marcescens has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6325. The serratia marcescens disclosed by the invention has a better function of killing the white ants without polluting the environment, and has the characteristics of environment friendliness and high efficiency.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to the application of Serratia marcescens in the control of termites. Background technique [0002] Termites are the oldest social insects on earth, with the characteristics of strong fecundity, high viability and great destructiveness. It is distributed on the six continents except Antarctica, and is mainly distributed between 45 degrees north and south latitude with the equator as the center. In my country, there are 476 known species of termites, more than 70 species that endanger housing and construction, and 19 major species of termites. The distribution of termites in my country is mainly in the south of the Huaihe River, gradually decreasing to the north, and gradually increasing to the south. There are records of distribution in most provinces of my country. [0003] At present, the control of termites is mainly based on chemical control. Because termites are social ins...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P7/04C12N1/20C12R1/43
Inventor 汤方李生臣
Owner 汤方
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