44 results about "Serratia rubidaea" patented technology

The invention relates to a method for cloning and expressing Serratia marcescens lipase by utilizing recombinant Bacillus subtilis, belonging to the fields of gene engineering and enzyme engineering. The method comprises the following steps of: firstly acquiring gene amplification of Serratia marcescens lipase (lipA), and then realizing overexpression of genes in type strain Bacillus subtilis 168 for the first time by utilizing pMA-5 plasmids. According to the method, Bacillus subtilis engineering strains which produce the Serratia marcescens lipase are constructed for the first time, after carrying out research on enzyme activities and fermenting properties of the strains and optimization on culture medium components and culture conditions, the activity of the lipase is 98.6 U / mL which is equal to 12 times of that, namely 8.57 U / mL, of a starting strain, and compared with the starting strain, the activity of the lipase is remarkably improved; and the method plays an active role in guiding the industrial production of the lipase by utilizing a microbiological fermentation method and can be applied to the field of food industry.

The invention discloses a method for quantitative determination of serratia marcescens. A serratia marcescens culture detection method is provided, a sampling loading volume best for uniform distribution of serratia marcescens on a nutrient agar plate is found by optimizing the sample loading volume in a plate count method, and the accuracy of a final plate count result is guaranteed. A computational formula suitable for a serratia marcescens quantitative determination result is also provided. Through analysis of the quantitative determination method and parameters in the detection result computational formula, the invention further provides an uncertainty evaluation method for quantitative determination of serratia marcescens. Serratia marcescens is an evaluation indicator bacterium for protective property of biological protection equipment for people, and evaluation accuracy and comparability can be guaranteed only through accurate quantitative determination.

A serratia marcescens strain T861 resistant to xanthomonas axonopodis pv. citri is preserved in CCTCC (china center for type culture collection) on 10, April, 2015, with the number of CCTCC M2015222. Fermentation liquor of the serratia marcescens strain T861 can suppress Xanthomonas axonopodis pv. citri; measurement by means of needle piercing shows that aseptic fermentation liquor of the serratia marcescens strain T861 has an in-vitro suppression rate for Xanthomonas axonopodis pv. citri being above 86.7%.

The invention discloses a serratia marcescens vaccine.The active ingredient of the serratia marcescens vaccine is a biological pure culture of serratia marcescens.The serratia marcescens is preserved in China General Microbiological Culture Collection Center on January 11, 2016 with the preservation number of CGMCC NO.11987.The invention further discloses a preparation method of the serratia marcescens vaccine.The serratia marcescens vaccine is prepared from the biological pure culture of the serratia marcescens through a proper separation method.According to the proper separation method, centrifugation is carried out at 4,000-6,000 rpm for 30-60 min, supernatant is discarded, sediment is inactivated, centrifugation is carried out again, supernatant is discarded, sediment is washed and centrifuged, and the serratia marcescens vaccine is obtained through preparation.The product produced through the preparation method is stable in yield, good in reproducibility and high in anti-tumor effect and spleen activity, and all other indexes meet the requirements of the quality standard.The serratia marcescens vaccine has obvious prevention and treatment effects on various solid tumors, and is an inactivated vaccine which is safe and free of toxic and side effects.

The invention discloses application of serratia marcescens in control of white ants and a key preparation technology. The serratia marcescens has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6325. The serratia marcescens disclosed by the invention has a better function of killing the white ants without polluting the environment, and has the characteristics of environment friendliness and high efficiency.

The invention discloses a Serratia marcescens engineering bacterium and its application in the production of prodigiosin, belonging to the field of biotechnology. The invention provides a Serratia marcescens engineering bacterium JNB5‑1ΔcpxR capable of high prodigiosin production, and the Serratia marcescens engineering bacterium JNB5‑1ΔcpxR is obtained by knocking out Serratia marcescens JNB5‑1 Inoculated with the gene encoding the response regulator protein CpxR, the Serratia marcescens engineered bacterium JNB5-1ΔcpxR was inoculated into the fermentation medium and fermented for 96 hours, and the prodigiosin production in the fermentation broth could be as high as 5.83g / L , 41.9% higher than that of wild-type Serratia marcescens JNB5‑1.

The invention provides a serratia marcescens anchorin duplicon and its use in host bacteria. The serratia marcescens anchorin duplicon has a sequence shown in the formula of SEQ ID NO: 2. The invention also provides an inhibition effect of the serratia marcescens anchorin duplicon in host bacteria. The invention also provides a use of the serratia marcescens anchorin duplicon in promotion of expression of phospholipase A1 in escherichia coli. The invention also provides a recombinant vector and a recombinant strain containing the serratia marcescens anchorin duplicon. An experiment proves that through the expression of the serratia marcescens anchorin duplicon, host bacteria can be inhibited obviously. The serratia marcescens anchorin duplicon can greatly promote high-activity expression of the phospholipase A1 in escherichia coli.

The invention discloses a strain of Serratia marcescens and its application. The name of the strain is Serratia marcescens subsp.sakuensis C273, and the preservation number is GDMCC NO: 61042. The strain was preserved in Building 59, Compound 59, No. 100 Xianlie Middle Road, Guangzhou City on June 3, 2020. The Guangdong Provincial Microbial Culture Collection Center. The present invention finds through experiments that the Serratia marcescens can remove cadmium ions in soil and water through co-metabolism, and can also reduce the pH in the system so as to dissolve insoluble phosphorus and potassium substances, illustrating that in the present invention Serratia marcescens has the potential to remove cadmium ions, increase soil available phosphorus and potassium content, and can be used for environmental pollution control and plant growth promotion.

The invention provides a serratia marcescens anchorin duplicon and its use in host bacteria. The serratia marcescens anchorin duplicon has a sequence shown in the formula of SEQ ID NO: 2. The invention also provides an inhibition effect of the serratia marcescens anchorin duplicon in host bacteria. The invention also provides a use of the serratia marcescens anchorin duplicon in promotion of expression of phospholipase A1 in escherichia coli. The invention also provides a recombinant vector and a recombinant strain containing the serratia marcescens anchorin duplicon. An experiment proves that through the expression of the serratia marcescens anchorin duplicon, host bacteria can be inhibited obviously. The serratia marcescens anchorin duplicon can greatly promote high-activity expression of the phospholipase A1 in escherichia coli.

The invention discloses a Serratia marcescens PS-1 strain isolated from Flea beetle flavus and application thereof. The present invention directly isolates a strain of Serratia marcescens (Serratia marcescens) strain from the body of the diseased flea beetle larva for the first time, which was preserved in the General Microbiology Center of China Microbiology Culture Collection Management Committee on July 22, 2013. The number is CGMCC No.7948. The Serratia marcescens PS-1 strain has strong pathogenicity to the larvae and adults of the flavonoid beetle and the larvae and adults of the beet armyworm, and can effectively control the development of the experimental population of the beet armyworm. The invention provides a technical basis for the field population control of the flea beetle and the beet armyworm, and is important for safely, effectively and sustainably controlling the flea beetle and the beet armyworm, and effectively ensuring the yield and quality of vegetables practical significance and practical value.

The invention discloses the application of Serratia marcescens in preparing termite repellent. The Serratia marcescens is a termite repellent prepared in the form of a Serratia marcescens strain culture solution. The present invention verifies that the Serratia marcescens strain culture solution can be used as a termite by mixing and applying the Serratia marcescens strain culture solution to the soil, after releasing the termites, and observing the repelling situation of the termites. The repellent is applied, and its repellent effect is persistent. And because Serratia marcescens is simple to cultivate, has a short growth period, a fast growth rate, and is easy to expand and cultivate, it is not a chemical agent, and has good conditions for application as a termite repellent.

The invention discloses recombinant Serratia marcescens with rcsB gene deletion and application thereof, belonging to the field of biotechnology. The invention provides a Serratia marcescens engineering bacterium JNB5-1ΔrcsB capable of high prodigiosin production, and the Serratia marcescens engineering bacterium JNB5-1ΔrcsB is obtained by knocking out Serratia marcescens JNB5-1 obtained from the gene encoding the transcriptional regulator RcsB, the Serratia marcescens engineering bacterium JNB5‑1ΔrcsB was inoculated into LB liquid medium for fermentation for 24 hours, and the production of prodigiosin in the fermentation broth could be as high as 116.48mg / L, 2.28 times that of wild-type Serratia marcescens JNB5‑1.