44 results about "Serratia rubidaea" patented technology

The invention relates to a serratia marcescens strain separated from phyllotreta striolata fabricius and applications thereof. A serratia marcescens strain is directly separated from phyllotreta striolata fabricius larva for the first time, and was preserved in China General Microbiological Culture Collection Center (CGMCC) in July 22th, 2013, with the preservation number of CGMCC No.7948. The serratia marcescens PS-1 strain has strong pathogenicity on the phyllotreta striolata fabricius larva and imagoes and laphygma exigua larva and imagoes, and can effectively control the development of laphygma exigua experiment populations. The serratia marcescens strain can provide technological base for the control on the field populations of phyllotreta striolata fabricius and laphygma exigua, and has important practical significance and practical values on safely, effectively and sustainably controlling the phyllotreta striolata fabricius and laphygma exigua and on efficiently ensuring the output and quality of vegetables.

The present invention relates to Serratia sp. A5 and applications thereof. The Serratia sp. A5 is preserved in the China General Microbiological Culture Collection Center on August 28, 2014, and the preservation number is CGMCC No.9621. According to the present invention, the Serratia sp. A5 is isolated from soil, the suitable growth temperature is 25-35 DEG C, the pH value is 7.0-7.5, and the Serratia sp. A5 has the lipase production ability; and the Serratia sp. A5 can be applied for degrading grease, relieving the inhibition effect of the grease in food waste anaerobic fermentation, increasing the biogas yield by 15-30%, and effectively increasing anaerobic fermentation hydrolysis and biogas production efficiency of other waste containing fat and the like or waste water, and further provides important significance for promotion of harmless, resource and energy utilization of organic waste.

The invention discloses application of serratia marcescens in control of white ants and a key preparation technology. The serratia marcescens has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6325. The serratia marcescens disclosed by the invention has a better function of killing the white ants without polluting the environment, and has the characteristics of environment friendliness and high efficiency.

The invention relates to a method for cloning and expressing Serratia marcescens lipase by utilizing recombinant Bacillus subtilis, belonging to the fields of gene engineering and enzyme engineering. The method comprises the following steps of: firstly acquiring gene amplification of Serratia marcescens lipase (lipA), and then realizing overexpression of genes in type strain Bacillus subtilis 168 for the first time by utilizing pMA-5 plasmids. According to the method, Bacillus subtilis engineering strains which produce the Serratia marcescens lipase are constructed for the first time, after carrying out research on enzyme activities and fermenting properties of the strains and optimization on culture medium components and culture conditions, the activity of the lipase is 98.6 U / mL which is equal to 12 times of that, namely 8.57 U / mL, of a starting strain, and compared with the starting strain, the activity of the lipase is remarkably improved; and the method plays an active role in guiding the industrial production of the lipase by utilizing a microbiological fermentation method and can be applied to the field of food industry.

The invention discloses a method for improving acid stress resistance of Serratia marcescens, and belongs to the technical field of gene engineering and microbial engineering. DNA (deoxyribonucleic acid) binding proteins XrpA are over-expressed in the Serratia marcescens to improve the acid stress resistance of the Serratia marcescens. The recombinant Serratia marcescens prepared by the method iscultured in an environment with the pH (potential of hydrogen) of 4.0 for 12 hours, and then the survival rate of the recombinant Serratia marcescens is 1.4 times that of wild Serratia marcescens.

The invention provides a preparation method of organic fertilizer special for corn. The preparation method includes following steps: 1), exposing and drying chicken manure under the sun, and well mixing with green tea powder, artemisia apiacea residue, garlic seedling and oyster shell powder to obtain a material A; 2), using a microbial agent E to ferment seafood leftover and selenium mineral powder for 30-35d, ultrasonically smashing, and adding zeolite powder to obtain a material C; 3), using to serratia rubidaea, ralstonia entropha, paenibacillus macquariensis, sphingomonas, azospirillumlipoferum, alcaligenes faecalis, bacillus thuringiensis and photosynthetic bacterium to ferment decomposed corn straw, the material A and the material C to obtain a material D; 4), putting the material Dinto a stirrer to be mixed with urea, calcium superphosphate, potassium chloride, borax and soil conditioner, granulating, drying, and coating to obtain the organic fertilizer. The organic fertilizerprepared by the method has the advantages of stable dissolution process and good prevention effect on various common corn diseases.

The invention belongs to the technical field of plant microbes, and in particular relates to serratia marcescens HBRM-16 and an application of the serratia marcescens HBRM-16 as a bacterial strain for promoting the growth of the roots of a rubber tree. According to the invention, ACC deaminase is taken as the main index, a serratia marcescens HBRM-16 strain is obtained through elaborate selection, and the serratia marcescens HBRM-16 strain is preserved in the China Center for Type Culture Collection (CCTCC); the experiment proves that the selected serratia marcescens HBRM-16 can be effectively taken as the strain for promoting the root growth, so that the growth of rubber tree seedlings is promoted, and the yield of natural rubber is improved.

The invention discloses Serratia marcescens strain capable of degrading chlorobenzene and application thereof. The Serratia marcescens strain has a preservation number of CCTCC NO: M 2019674, and is named Serratia marcescens TF-1. The Serratia marcescens TF-1 can degrade the chlorobenzene by taking the chlorobenzene as the sole carbon source and energy, as well as carrying out co-metabolism. In addition, the Serratia marcescens TF-1 is capable of maintaining relatively high activity in both poor-breeding polluted fields and compound polluted fields, and is suitable for application in the fields of waste water, drinking water, soil remediation and the like. Therefore, new breakthroughs can be expectedly made in the field of engineering application of biodegradation of chlorinated aromatic hydrocarbons.

The invention discloses a serratia marcescens engineering bacterium and application thereof in production of prodigiosin, and belongs to the technical field of biology. The invention provides a serratia marcescens engineering bacterium JNB5-1 delta cpxR capable of realizing high yield of prodigiosin. The serratia marcescens engineering bacterium JNB5-1 delta cpxR is obtained by knocking out a genefor coding response regulatory protein CpxR in serratia marcescens JNB5-1. After the serratia marcescens engineering bacterium JNB5-1 delta cpxR is inoculated into a fermentation culture medium and fermented for 96 hours, the yield of prodigiosin in fermentation liquor can be as high as 5.83 g / L and is improved by 41.9% compared with that of wild serratia marcescens JNB5-1.

The invention discloses gene-rcsB-deficient recombinant Serratia marcescens and application thereof and belongs to the technical field of biology. The invention provides a Serratia marcescens engineering strain JNB5-1[delta]rcsB capable of high yielding prodigiosin. The Serratia marcescens engineering strain JNB5-1[delta]rcsB is obtained through knocking out a gene encoding a transcriptional regulation factor RcsB from Serratia marcescens JNB5-1. By inoculating an LB liquid culture medium with the Serratia marcescens engineering strain JNB5-1[delta]rcsB and carrying out fermentation for 24h, the yield of the prodigiosin in fermentation broth can reach up to 116.48mg / L and is 2.28 times that of wild type Serratia marcescens JNB5-1.

The present invention discloses a CRISPR-Cas9 dual vector system suitable for genetic modification of serratia. The CRISPR-Cas9 dual vector system comprises a vector pCAS-p15A-sacB delta lambda-Red and a vector pTarget F-Gm. The CRISPR-Cas9 dual vector system makes up for limitations of a serratia gene editing system and provides a platform for verification of serratia gene functions and a research on a mechanism of production of secondary metabolites of the serratia strain. The constructed CRISPR-Cas9 dual vector system is used to conduct gene luxS knock out and replenishment experiments anda preliminary study is made on a co-regulatory mechanism of serratia YD25 secondary metabolite prodigiosin and Serrawettin W2. A positive regulation of a gene luxS on synthesis of the prodigiosin andSerrawettin W2 is determined.

The invention discloses a multi-purpose method of serratia marcescens strains, and belongs to the technical field of fermentation engineering. The components of a fermentation culture medium are adjusted, and metabolism products of a serratia marcescens SDSPY136 strain are respectively 2-Keto-D-gluconic acid and prodigiosins, so that multiple purposes of the serratia marcescens SDSPY136 strain arerealized. In the step (2), the content of the 2-Keto-D-gluconic acid in fermentation liquid is very high, and byproducts are very few, so that in a 2KGA fermentation culture medium, the specificity of the 2-Keto-D-gluconic acid produced by metabolism of the serratia marcescens SDSPY136 strain is very high, and the purification is convenient. In the step (3), the content of prodigiosin in the fermentation liquid is very high, and byproducts are very few, so that in the prodigiosin fermentation culture medium, the specificity of the prodigiosin produced by metabolism of the serratia marcescensSDSPY136 strain is very high, and the purification is convenient.

The invention discloses a method for quantitative determination of serratia marcescens. A serratia marcescens culture detection method is provided, a sampling loading volume best for uniform distribution of serratia marcescens on a nutrient agar plate is found by optimizing the sample loading volume in a plate count method, and the accuracy of a final plate count result is guaranteed. A computational formula suitable for a serratia marcescens quantitative determination result is also provided. Through analysis of the quantitative determination method and parameters in the detection result computational formula, the invention further provides an uncertainty evaluation method for quantitative determination of serratia marcescens. Serratia marcescens is an evaluation indicator bacterium for protective property of biological protection equipment for people, and evaluation accuracy and comparability can be guaranteed only through accurate quantitative determination.

A serratia marcescens strain T861 resistant to xanthomonas axonopodis pv. citri is preserved in CCTCC (china center for type culture collection) on 10, April, 2015, with the number of CCTCC M2015222. Fermentation liquor of the serratia marcescens strain T861 can suppress Xanthomonas axonopodis pv. citri; measurement by means of needle piercing shows that aseptic fermentation liquor of the serratia marcescens strain T861 has an in-vitro suppression rate for Xanthomonas axonopodis pv. citri being above 86.7%.

The invention discloses a BVG90 _ 08615 gene deleted serratia marcescens engineering bacterium, and belongs to the technical field of biology. The invention provides a serratia marcescens engineeringstrain JNB5-1 [delta] BVG90 _ 08615 capable of highly yielding prodigiosin. The serratia marcescens engineering strain JNB5-1 [delta] BVG90 _ 08615 is a serratia marcescens strain. The serratia marcescens engineering bacterium JNB5-1 delta BVG90 _ 08615 is obtained by knocking out a gene for coding a transcription regulation factor BVG90 _ 08615 in the serratia marcescens JNB5-1. The serratia marcescens engineering strain JNB5-1 delta BVG90 _ 08615 is inoculated into an LB liquid culture medium to be fermented for 24 h, and the yield of prodigiosin in fermentation liquor can reach 58.68 mg / L and is 1.15 times that of wild serratia marcescens JNB5-1.

The invention discloses a serratia marcescens vaccine.The active ingredient of the serratia marcescens vaccine is a biological pure culture of serratia marcescens.The serratia marcescens is preserved in China General Microbiological Culture Collection Center on January 11, 2016 with the preservation number of CGMCC NO.11987.The invention further discloses a preparation method of the serratia marcescens vaccine.The serratia marcescens vaccine is prepared from the biological pure culture of the serratia marcescens through a proper separation method.According to the proper separation method, centrifugation is carried out at 4,000-6,000 rpm for 30-60 min, supernatant is discarded, sediment is inactivated, centrifugation is carried out again, supernatant is discarded, sediment is washed and centrifuged, and the serratia marcescens vaccine is obtained through preparation.The product produced through the preparation method is stable in yield, good in reproducibility and high in anti-tumor effect and spleen activity, and all other indexes meet the requirements of the quality standard.The serratia marcescens vaccine has obvious prevention and treatment effects on various solid tumors, and is an inactivated vaccine which is safe and free of toxic and side effects.

The invention discloses application of serratia marcescens in control of white ants and a key preparation technology. The serratia marcescens has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6325. The serratia marcescens disclosed by the invention has a better function of killing the white ants without polluting the environment, and has the characteristics of environment friendliness and high efficiency.

The invention provides a method for preparing recombinant serratia marcescens nuclease. The method comprises the following steps: carrying out fusion expression on coding nucleic acid of the serratia marcescens nuclease and coding nucleic acid of a mutant SUMO3 tag, and separating to obtain the serratia marcescens nuclease. The yield of the protein is improved by optimizing prokaryotic expression; through mutation modification of SUMO3, the renaturation rate and uniformity of the recombinant serratia marcescens nuclease are improved. Meanwhile, the purification steps are simplified, the production cost is reduced, and the method is suitable for large-scale industrial production of the recombinant serratia marcescens nuclease.

The invention discloses a Serratia marcescens engineering bacterium and its application in the production of prodigiosin, belonging to the field of biotechnology. The invention provides a Serratia marcescens engineering bacterium JNB5‑1ΔcpxR capable of high prodigiosin production, and the Serratia marcescens engineering bacterium JNB5‑1ΔcpxR is obtained by knocking out Serratia marcescens JNB5‑1 Inoculated with the gene encoding the response regulator protein CpxR, the Serratia marcescens engineered bacterium JNB5-1ΔcpxR was inoculated into the fermentation medium and fermented for 96 hours, and the prodigiosin production in the fermentation broth could be as high as 5.83g / L , 41.9% higher than that of wild-type Serratia marcescens JNB5‑1.

The invention provides a serratia marcescens anchorin duplicon and its use in host bacteria. The serratia marcescens anchorin duplicon has a sequence shown in the formula of SEQ ID NO: 2. The invention also provides an inhibition effect of the serratia marcescens anchorin duplicon in host bacteria. The invention also provides a use of the serratia marcescens anchorin duplicon in promotion of expression of phospholipase A1 in escherichia coli. The invention also provides a recombinant vector and a recombinant strain containing the serratia marcescens anchorin duplicon. An experiment proves that through the expression of the serratia marcescens anchorin duplicon, host bacteria can be inhibited obviously. The serratia marcescens anchorin duplicon can greatly promote high-activity expression of the phospholipase A1 in escherichia coli.

The invention provides a polypeptide having serrapeptase activity. The polypeptide has the gene sequence shown in SEQ ID NO:3 and the amino acid sequence shown in SEQ ID NO:4. A serrapeptase gene is cloned from serratia, the gene is cloned into a pHT43 vector by a genetic engineering technology to construct an expression vector, and a constructed expression plasmid is led into different expressionstrains through conversion; through culture and induced expression, the strain can express the polypeptide having high serrapeptase activity. The polypeptide having high serrapeptase activity is expressed in bacillus by the gene cloning technology. The strain culture cycle is shortened, the production cost is reduced, the product purity is higher, and the extraction process is simpler. The polypeptide has great application prospect and commercial value in the biomedical technical field.

The invention discloses a strain of Serratia marcescens and its application. The name of the strain is Serratia marcescens subsp.sakuensis C273, and the preservation number is GDMCC NO: 61042. The strain was preserved in Building 59, Compound 59, No. 100 Xianlie Middle Road, Guangzhou City on June 3, 2020. The Guangdong Provincial Microbial Culture Collection Center. The present invention finds through experiments that the Serratia marcescens can remove cadmium ions in soil and water through co-metabolism, and can also reduce the pH in the system so as to dissolve insoluble phosphorus and potassium substances, illustrating that in the present invention Serratia marcescens has the potential to remove cadmium ions, increase soil available phosphorus and potassium content, and can be used for environmental pollution control and plant growth promotion.

The invention discloses a method for improving serratia marcescens to synthesize prodigiosin through an overexpressed gene psrB, and belongs to the technical field of gene engineering and microbial engineering. According to the invention, the prodigiosin synthesis capability of the serratia marcescens is remarkably improved by overexpressing an encoding gene BVG90_04085 (psrB) of a DeoR family transcriptional regulation factor PsrB (BVG90_04085) in the serratia marcescens; when the recombinant serratia marcescens prepared by the method disclosed by the invention is fermented for 72 hours in a fermentation culture medium to produce prodigiosin; the prodigiosin production capacity of the recombinant strain JNB5-1-PsrB is improved by 28.33% compared with that of a wild strain JNB5-1; and the yield reaches 6.84 g / L.

The invention belongs to the technical field of microorganism growth promotion, and particularly relates to serratia rubidaea with a growth promotion effect and an application of the serratia rubidaea. The soil salinization problem seriously affects the crop yield and the sustainable development of agricultural economy. Aiming at the development problem of saline alkali soil, some root growth-promoting bacteria can improve the salt resistance and alkali resistance of crops, promote the growth condition of the crops, improve the stress resistance of the crops and gradually realize the soil improvement effect. The invention provides a serratia rubidaea strain 3004 J-3. Tests prove that the strain can keep good growth vigor in a saline-alkali environment, has the capacity of promoting growthtraits of plants in the saline-alkali environment, further explores the mechanism of the plants, has the effects of producing protease and siderophore and decomposing organophosphorus in the environment, and has important significance in saline-alkali soil improvement and plant stress resistance improvement.

The invention provides a serratia marcescens anchorin duplicon and its use in host bacteria. The serratia marcescens anchorin duplicon has a sequence shown in the formula of SEQ ID NO: 2. The invention also provides an inhibition effect of the serratia marcescens anchorin duplicon in host bacteria. The invention also provides a use of the serratia marcescens anchorin duplicon in promotion of expression of phospholipase A1 in escherichia coli. The invention also provides a recombinant vector and a recombinant strain containing the serratia marcescens anchorin duplicon. An experiment proves that through the expression of the serratia marcescens anchorin duplicon, host bacteria can be inhibited obviously. The serratia marcescens anchorin duplicon can greatly promote high-activity expression of the phospholipase A1 in escherichia coli.

The invention discloses a Serratia marcescens PS-1 strain isolated from Flea beetle flavus and application thereof. The present invention directly isolates a strain of Serratia marcescens (Serratia marcescens) strain from the body of the diseased flea beetle larva for the first time, which was preserved in the General Microbiology Center of China Microbiology Culture Collection Management Committee on July 22, 2013. The number is CGMCC No.7948. The Serratia marcescens PS-1 strain has strong pathogenicity to the larvae and adults of the flavonoid beetle and the larvae and adults of the beet armyworm, and can effectively control the development of the experimental population of the beet armyworm. The invention provides a technical basis for the field population control of the flea beetle and the beet armyworm, and is important for safely, effectively and sustainably controlling the flea beetle and the beet armyworm, and effectively ensuring the yield and quality of vegetables practical significance and practical value.

The invention discloses the application of Serratia marcescens in preparing termite repellent. The Serratia marcescens is a termite repellent prepared in the form of a Serratia marcescens strain culture solution. The present invention verifies that the Serratia marcescens strain culture solution can be used as a termite by mixing and applying the Serratia marcescens strain culture solution to the soil, after releasing the termites, and observing the repelling situation of the termites. The repellent is applied, and its repellent effect is persistent. And because Serratia marcescens is simple to cultivate, has a short growth period, a fast growth rate, and is easy to expand and cultivate, it is not a chemical agent, and has good conditions for application as a termite repellent.

The invention discloses serratia marcescens and an application of the serratia marcescens in degradation of tetrabromobisphenol A. The serratia marcescens is separated from a circulating system for treating tetrabromobisphenol A simulated wastewater, a strain of serratia marcescens which has superior degradation capability on tetrabromobisphenol A is separated from an immobilized biological membrane in an operation system, is named as TB-3, is preserved in China Center for Type Culture Collection on April 1, 2022, and has the preservation number of CGMCC NO.3487. The invention also discloses a method for preparing the serratia marcescens. The preservation number of the strain is CCTCC M 2022367. The strain has relatively strong tetrabromobisphenol A degradation capability; tetrabromobisphenol A can be used as a unique carbon source for decomposition and utilization; meanwhile, the strain is high in activity, the culture method is simple, and the strain can be directly used for treating the environment containing tetrabromobisphenol A; good application prospects are realized.

The invention discloses recombinant Serratia marcescens with rcsB gene deletion and application thereof, belonging to the field of biotechnology. The invention provides a Serratia marcescens engineering bacterium JNB5-1ΔrcsB capable of high prodigiosin production, and the Serratia marcescens engineering bacterium JNB5-1ΔrcsB is obtained by knocking out Serratia marcescens JNB5-1 obtained from the gene encoding the transcriptional regulator RcsB, the Serratia marcescens engineering bacterium JNB5‑1ΔrcsB was inoculated into LB liquid medium for fermentation for 24 hours, and the production of prodigiosin in the fermentation broth could be as high as 116.48mg / L, 2.28 times that of wild-type Serratia marcescens JNB5‑1.

The invention discloses a serratia marcescens HL1 strain for treating Alzheimer's disease and an application. The strain is separated from wild huperzia serrata endophytic bacteria and belongs to the serratia marcescens HL1 strain, and the amino sequence of the serratia marcescens HL1 strain is shown as SEQ ID NO: 1. The serratia marcescens HL1 strain is used as a biological strain for treating the Alzheimer's disease, the excellent huperzine A-producing high-efficiency expression gene of the serratia marcescens HL1 strain is transferred into other types of strains, more excellent characters are obtained through gene modification, and in addition, the strain can be further optimized through mutation breeding. Research of the serratia marcescens HL1 strain has great significance in biosynthesis industrial production of huperzine A, protection of plant resources, solving of clinical first-line medication requirements and reduction of medical cost for treating Alzheimer's disease.