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A serratia marcescens strain producing high-purity 2-keto-D-gluconic acid

A technology of Serratia marcescens and gluconic acid, applied in the field of microorganisms, can solve the problems of low conversion rate, bacteriophage contamination, low purity and the like

Active Publication Date: 2015-08-12
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems of low conversion rate, low purity, large production loss and easy contamination by bacteriophage in the production of 2-keto-D-gluconic acid by the fermentation method in the prior art, the present invention provides a high-purity Serratia marcescens strain of 2-keto-D-gluconic acid

Method used

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  • A serratia marcescens strain producing high-purity 2-keto-D-gluconic acid
  • A serratia marcescens strain producing high-purity 2-keto-D-gluconic acid
  • A serratia marcescens strain producing high-purity 2-keto-D-gluconic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 Acquisition of Serratia marcescens CGMCC No.10548 bacteria

[0072] Serratia marcescens (Serratia marcescens) SDSPY-136 strain producing high-purity 2-keto-D-gluconic acid has been preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and its preservation number is CGMCC No. : 10548, and its preservation time is: February 9, 2015.

[0073] The collection strain of the present invention, that is, the Serratia marcescens SDSPY-136 strain producing high-purity 2-keto-D-gluconic acid, the acquisition steps are as follows:

[0074]1) The microorganisms in the garden soil sample were inoculated in the enrichment medium. After enrichment culture, they were serially diluted, and then spread on bromocresol purple-calcium carbonate screening plates, and cultured at 30°C for 1-2 days; The single colony with a large circle was transferred to the slant medium for 1-2 days at 30°C, and then the shake flask fermentation wa...

Embodiment 2

[0088] Example 2 Biological characteristics, physiological and biochemical characteristics and 16S rRNA gene sequencing of Serratia marcescens CGMCC No. 10548 strain

[0089] A The biological characteristics of this preserved strain are: observed after cultured on a solid culture plate (separation medium) at 30°C for 24 hours, the colony can form a red round colony with a smooth and moist surface, slightly raised (such as figure 1 , figure 2 shown), under the optical microscope, the bacteria were nearly spherical and short rod-shaped, Gram staining was negative, and there were no spores (such as image 3 shown). The composition of the solid medium for bacterial morphology observation (g / L): peptone 5, sodium chloride 5, beef extract 3, agar 18, pH 7.0. For the experimental method of observing the above morphological characteristics, refer to "Handbook of Common Bacterial System Identification" edited by Dong Xiuzhu, Cai Miaoying, etc., Science Press, 2001, first edition...

Embodiment 3

[0133] Example 3 Application of Serratia marcescens CGMCC No.10548 strain

[0134] Medium:

[0135] The composition of the slant medium is (g / L): peptone 5, sodium chloride 5, beef extract 3, agar 20, pH 7.0;

[0136] The composition of the liquid seed medium is (g / L): peptone 5, sodium chloride 5, beef extract 3, pH 7.0;

[0137] The composition of the fermentation medium is (g / L): glucose 110, corn steep liquor 10, KH 2 PO 4 1. MgSO 4 ·7H 2 O 0.8, FeSO 4 ·7H 2 O 0.036, CaCO 3 50, pH7.0.

[0138] Method for producing 2-keto-D-gluconic acid using the collection strain

[0139] 1) Serratia marcescens (Serratia marcescens) CGMCC N0.10548 strain was transferred to the slant medium for activation;

[0140] Prepare the seed medium according to the composition of the above-mentioned liquid seed medium. The liquid volume in a 250ml triangular bottle is 30ml, steam sterilize at 121°C for 20 minutes, cool to room temperature, inoculate 2 rings of slant strains, set the ...

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Abstract

The invention discloses a serratia marcescens strain producing high-purity 2-keto-D-gluconic acid, and belongs to the field of microorganisms. The strain is a serratia marcescens SDSPY-136 strain, is deposited in the China General Microbiological Culture Collection Center in February 9, 2015, and has an accession number of CGMCC No:10548. The strain can adopts glucose and trehalose as carbon sources, cannot ferment arabinos, is free of gas generation during glucose fermentation and is positive to catalase. The strain is used for production of the 2-keto-D-gluconic acid. Production of the 2-keto-D-gluconic acid by the strain is high in glucose conversion ratio and less in by-products, and the obtained 2-keto-D-gluconic acid is high in purity.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Serratia marcescens strain producing high-purity 2-keto-D-gluconic acid. Background technique [0002] 2-keto-D-gluconic acid (2-keto-D-gluconic acid, 2KGA) is an important derivative of gluconic acid. 2-Keto-D-gluconic acid can be used as a calcium-enriched additive for feed, a detergent booster, a water machine plasticizer, and a photographic developer. At the same time, 2-keto-D-gluconic acid is also a precursor for the synthesis of furfural, D-arabinose, D-isoascorbic acid and sodium D-isoascorbate. Among them, the synthesis of D-isoascorbic acid (sodium) is its most important use. [0003] Erythorbic acid is white to light yellow crystal or crystalline powder, odorless, sour taste, strong reducibility, easily soluble in water, widely used in food, medicine, textile, building materials, daily chemical industry and other industries, among which , the most important use is in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00C12P7/60C12R1/43
Inventor 徐慧刘建军田延军李文靖孙文涛王勇
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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