Bacteria cellulose producing bacteria and method for preparing bacteria cellulose using above bacterial strain

Abstract

The invention provides bacterial cellulose (BC) producing bacteria and a method for preparing the bacterial cellulose by using the bacterial strain. The bacterial strain is a selected gluconic acid acetobacter xylinum GD-BC-1 with an accession number of CCTCC M 208149. The method is to use the bacterial strain as a fermentation bacterial strain and use glucose as a main fermentation material for fermentation so as to obtain the BC. The preparation method comprises the following steps of: activating a slant bacterial strain, liquid-activating the slant bacterial strain, inoculating the liquid-activated slant bacterial strain in a fermentation culture medium to perform dynamic or static fermentation and extracting products of BC from the fermentation solution. The preparation method uses the bacterial strain of gluconic acid acetobacter xylinum GD-BC-1 with the accession number of CCTCC M 208149 for fermentation so as to produce the BC. The BC can be produced through dynamic or static fermentation in a conical flask with glucose as the main fermentation material, and the yield is high and different methods can be used in different production environment to produce BC.

Description

technical field [0001] The invention relates to a microbial strain for producing bacterial cellulose and a method for fermenting and producing bacterial cellulose by using the bacterial strain. Background technique [0002] Cellulose is the most abundant biopolymer on the earth, and it is the main component of plant biomass. The cellulose produced by plants on the earth reaches hundreds of millions of tons every year. In addition to plants, some lower animals can produce animal cellulose (Tunlcin), and some bacteria can produce extracellular bacterial cellulose (Bacterial cellulose, hereinafter referred to as BC) in a heterotrophic manner more efficiently and with higher purity than plants. In 1886, A.J.Brown firstly reported that Acetobacter xylinum could synthesize an extracellular gel-like substance (A.J.Brown.On an aceticferment forms cellulose[J].J.Chem.Soc, 1886, 49 : 432-439.), but due to the lack of suitable experimental methods and the low yield of cellulose cords,...

AI Technical Summary

Problems solved by technology

The dynamic fermentation method consumes relatively high energy and the process is more complicated, but it is more suitable for large-scale production, and the yield of bacterial cellulose may be higher; the static fermentation method consumes less energy and has a simple process, but the yield may not be high, and is more suitable for small-scale production Production

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