Composite fungus agent for preventing blight of vegetable in greenhouse

A compound bacterial agent and vegetable technology, which is applied in biocides, animal repellents, plant growth regulators, etc., can solve the problems of biological control agents for epidemic diseases that have not yet appeared, and achieve the effects of improving control effects, increasing income, and saving expenses

Active Publication Date: 2005-01-26
NANJING XINGUOYUAN ECOLOGICAL AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no biocontrol agent with high control effect on the disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: get by bacterial strain B130, FH17, BB11, J1, J2, the wet bacterium that J3 mixing ratio is 1: 0.5: 0.2: 0.3: 0.3: 0.2, every gram of wet bacterium contains 4 * 10 10 Add thalline preservation solution sodium borate buffer to a final concentration of 0.01mol / L (pH7.3), add Tween20 to a final concentration of 0.001mol / L to obtain a composite bacterial agent (trade name: PGPR composite Bacteria), can be stored at room temperature for 2 to 3 years, used for pepper blight biocontrol experiments (Table 1), the results show that the average control effect of the use of the blight on vegetable blight can reach 81.3%, and the increase in production can reach 96.4%, obviously Higher than the control group and the above strains of single bacteria preparations.

[0028] Solution

Embodiment 2

[0029] Embodiment 2: get by bacterial strain B130, FH17, BB11, J1, J2, the wet bacterium that J3 mixing ratio is 1: 0.4: 0.2: 0.3: 0.3: 0.3 obtains, every gram of wet bacterium contains 4 * 10 15 Each thalline is added to the sodium borate buffer solution of the thalline preservation solution to a final concentration of 0.01mol / L (pH7.3), and Tween20 is added to a final concentration of 0.001mol / L to obtain a composite bacterial agent (the trade name is PGPR composite bacteria) bacterium), can be preserved at room temperature for a long time, and is used for epidemic disease biocontrol experiments (table 2, table 3). The control group and the single bacterial preparations of the above strains.

[0030] Solution

Incidence rate (%)

Anti-efficacy (%)

Yield increase rate (%)

control group

Watering every time after transplanting

22.9

test group

Composite bacterial agent program 1 treatment

1.2

95.6

48.1

B.ci...

Embodiment 3

[0033] Embodiment 3: get by bacterial strain B130, FH17, BB11, J1, J2, the wet bacterium that J3 mixing ratio is 1: 0.4: 0.3: 0.2: 0.2: 0.3 obtains, every gram of wet bacterium contains 4 * 10 14 A bacterial cell is added to the sodium borate buffer solution of the bacterial cell preservation solution to a final concentration of 0.01mol / L (pH7.3), and Tween20 is added to a final concentration of 0.001mol / L to obtain a composite bacterial agent, which can be stored at room temperature for a long time. In the capsicum blight biocontrol experiment (Table 4, Table 5), the results show that the average control effect of the use of this bacterial agent on cucumber blight can reach 87.8%, and the increase in production can reach 247.5%, which is significantly higher than that of the control group and above each strain single bacteria preparation.

[0034] Solution

[0035] Solution

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PUM

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Abstract

A biological control bacteria formulation for preventing green house vegetable disease comprises Bacillus circulans, Bacillus pumilus, Bacillus cereus, Bacillus Subtilis, Serratia marcescens, Pseudomonas fluorescens. The preparation method is : mixing the above-mentioned bacteria according to 1:0.4-0.5:0.2-0.3:0.2-0.3:0.2-0.3:0.2-0.3, 200rpm shaking 36 hours in PDA culture liquid in 33 DEG C, 7000rpm centrifuging, preparing formulation using mixed bacteria and bacteria preservative fluid according to 1:40(g/ml), the alive bacteria concentration being 1x10<9>-1x10<14>cfu/ml. The bacteria formulation can be used in dunghill, root irrigation while transplanting after dilution. The preventing effect of bacteria formulation to vegetable disease can reach 75%, yield promoting effect is more than 45%.

Description

1. Technical field [0001] The compound bacteria agent (PGPR bacteria agent) for preventing and treating greenhouse vegetable blight belongs to the technical field of crop disease prevention and treatment, and is specially used for pollution-free prevention and control of vegetable blight. 2. Technical background [0002] At present, the planting area of ​​vegetables in protected areas in my country is constantly expanding. Due to the temperature and humidity conditions in the protected areas are suitable for the growth of Phytophthora, coupled with the continuous cropping of the same species, the disease has increased year by year. At present, chemical pesticides are mainly used for the prevention and control of vegetable diseases, such as: fofosalum, metalaxyl, prequel, amixida, etc. However, the use of chemical pesticides will not only produce pesticide residues, but also gradually increase the resistance of Phytophthora with the use of chemical pesticides year by year. Fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02
Inventor 郭坚华蒋志强
Owner NANJING XINGUOYUAN ECOLOGICAL AGRI SCI & TECH
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