Broad-spectrum fungus-resistant bacillus and application thereof to preventing wheat scab
A wheat scab and bacillus technology, applied in the application, bacteria, fungicides and other directions, can solve problems such as failure of wheat scab prevention and control, and achieve the effect of being conducive to pollution-free production and improving quality
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Embodiment 1
[0027] Example 1: Isolation and screening of Bacillus sp. DY26-010
[0028] The present invention relates to Bacillus sp. DY26-010 obtained by screening from sediment samples collected by the applicant from the 26th scientific investigation of China Ocean. The bacterium grows on LB medium, and after 24 hours of culture at 28°C, the bacterium is light yellow, translucent, with neat edges, smooth and shiny surface, and the colony morphology can be seen in figure 1 .
Embodiment 2
[0029] Embodiment 2: Bacillus sp. (Bacillus sp.) DY26-010 strain identification
[0030] Using the total DNA of Bacillus spp. DY26-020 as a template, its 16S rDNA sequence was amplified with bacterial universal primers. Universal primer 27F: AGAGTTTGATCCTGGCTCAT; 1492R: ACGGCTACCTTGTTACGACTT. The PCR reaction conditions were: denaturation at 94°C for 1 min; annealing at 55°C for 1 min; extension at 72°C for 1.5 min, 30 cycles. After the amplified sequence was sequenced by a sequencing company, the 16S rDNA sequence of the strain was obtained. The sequence was compared with the nucleic acid data in the National Center for Biotechnology Information (NCBI) GeneBank (http: / / ncbi.nlm.nih.gov / blast). The comparison results showed that the sequence of DY26-01016SrDNA was similar to Bacillus amyloliquefaciens (JQ245705.1), Bacillus amyloliquefaciens (HQ844506.1), Bacillus methylotrophicus (KC790301.1), Bacillus amyloliquefaciens (KC478951.1), Bacillus amyloliquefaciens (4J5J0. It is...
Embodiment 3
[0031] Embodiment 3: the mensuration of phytopathogenic fungi antimicrobial spectrum
[0032]The inhibitory activity of Bacillus sp. DY26-010 against 10 plant pathogenic fungi was determined by disc method. The tested pathogenic fungi were made into a fungus dish with a sterile puncher, inoculated in the center of a potato solid medium (PDA) plate, and cultured at 28°C. After the mycelium of the pathogenic fungus to be tested spreads and grows, a sterilized double-layer filter paper sheet (diameter 6 mm) is placed around the bacterial block, and the filter paper sheet is about 1 cm away from the edge of the mycelium.
[0033] After Bacillus sp. DY26-010 of the present invention was cultured on a shaker for 2 days, it was centrifuged at 10,000 rpm for 10 min, filtered and sterilized to make a sterile fermentation supernatant, which was added dropwise on each filter paper sheet, 50 μL / filter paper sheet. Continue culturing at 28°C for 24 hours, and observe whether the growth of...
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