The invention relates to a
rapid detection method for content of
adenosine in
paecilomyces hepialid
mycelium powder. The
rapid detection method is characterized by comprising the following steps: 1) preparing
adenosine standard solution, namely weighing an
adenosine standard substance, dissolving and carrying out constant volume with adenosine extracting solution, and preparing the adenosine
standard solution for later use; 2) preparing sample solution, namely weighing a
paecilomyces hepialid
mycelium powder sample, adding the adenosine extracting solution, dissolving, uniformly mixing, addingquartz sand and
grinding to be pasty, cooling, then transferring into a colorimetric tube, carrying out constant volume, carrying out
ultrasonic oscillation by virtue of an ultrasonic oscillator, mixing, freezing in an environment at the temperature of -20 DEG C, then melting and transferring into a centrifugal
machine to be subjected to centrifugal treatment, taking supernatant, filtering by virtue of a 0.45Mum
filtration membrane, and preparing the sample solution for later use; and 3) carrying out detection and analysis by virtue of
high performance liquid chromatography. The
rapid detection method provided by the invention is scientific and reasonable in design, good in adenosine chromatographic peak separation effect, simple in mobile phase, high in adenosine
recovery rate and good in
repeatability and has the advantages of easy operation, shorter time, high detection speed and high detection efficiency.