59 results about "Paecilomyces variotii" patented technology

The invention relates to bacillus subtilis Pc3 and a use of bacillus subtilis Pc3 in preparation of a fermentation supernatant for preventing and controlling plant pathogenic fungi, and relates to bacilli. The bacillus subtilis Pc3 has a preservation number of CCTCC NO: M2013470. The bacillus subtilis Pc3 can prevent and control plant pathogenic fungi such as gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani and fusaria. A rape sclerotinia sclerotiorum prevention and control pot experiment proves that the bacillus subtilis Pc3 has good biological-prevention and control effects and has prevention and control efficiency of 95.6%. The fermentation supernatant of the bacillus subtilis Pc3 can be prepared by simple processes and has a wide fungus inhibition spectrum and good biological prevention effects.

The present invention relates to a paecilomyces genus microorganism and microbial Mixing insecticide for controlling the soil pests using the same, more particularly to a paecilomyces genus microorganism having insecticidal activity to soil pests and a microbial insecticide for controlling the soil pests containing the same. Since the microbial insecticide of the present invention shows excellent insecticidal activity to harmful soil pests, it can effectively be used as a environment-compatible insecticide to prevent the soil pests from farmland crops.

The invention relates to bacilli, in particular to a bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi. A preservation number of the Bacillus sp.DY26-004 is CCTCC NO:M 2015406. The bacillus DY26-004 has a strong inhibiting effect on various plant pathogenic fungi such as fusaria, colletotrichum musae, paecilomyces variotii, sclerotinia scleotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani, alternaria alternata, botrytis cinerea and colletotrichum gloeosporioides, and has good biological prevention and control effects in pot experiments for prevention and control of sclerotinia scleotiorum and fusarium graminearum, wherein control efficiencies are 72% and 65% respectively. The bacillus DY26-004 which is a nontoxic, non-pathogenic and environment-friendly bacillus is a useful resource for research and development of microbial pesticides and has a potential application prospect.

The invention relates to a new microbiological species, the phorozoon is Paecilomyces suffultus (Petch) Samson. The method consists of inoculating the said germ to parasite body or subculture medium, cultivating them in a proper condition to obtain a yellow-while fruiting body of Cordyceps. The said germ has advantages that it is suitable for artificial culture in a large number, the effective composition content is high, the adaptive capacity for environment is strong, the production velocity of bacterium is rapid, the stress is strong, the yield rate is high, the cultivated cycle is short and the germ can not degenerate.

The invention discloses epipodium polycyclic aromatic hydrocarbons degrading bacteria and the application thereof. The epipodium polycyclic aromatic hydrocarbons degrading bacteria (paecilomyces variotii) F6 provided by the invention has the function of pipodium polycyclic aromatic hydrocarbons and pipodium polycyclic aromatic hydrocarbons mixture. After the pipodium polycyclic aromatic hydrocarbons is cultured for 30 d in a polycyclic aromatic hydrocarbons compound system, the degrading rate to the polycyclic aromatic hydrocarbons becomes 16.06 percent to 24.57 percent; however, the degrading rate in a single polycyclic aromatic hydrocarbons compound system becomes 10.42 percent to 33.25 percent, wherein, the degrading rate of benzo (k) fluoranthene and benzo (b) fluoranthene is the highest, which respectively accounts for 33.25 percent and 26.44 percent. The strain provided by the invention can supply novel germplasm resource for developing the co-metabolism mechanism study of a plurality of epipodium polycyclic aromatic hydrocarbons and the construction of aromatic hydrocarbons combined pollution water-soil environment repairing technology.

The invention relates to Antarctic-derived Bacillus sp.N311 and an application of Bacillus sp.N311 for preventing and treating phytopathogenic fungi, which relate to Bacillus sp. A preservation number of the Bacillus sp.N311 is CCTCC NO: M 2015607. The Bacillus sp.N311 can be used for preparing a fermentation supernatant for preventing and treating phytopathogenic fungi. The phytopathogenic fungi comprise fusarium, gloeosporium musarum, Paecilomyces variotii, sclerotinia sclerotiorum, Alternaria longipes, Trichoderma viride, Rhizoctonia solani, alternaria alternate, botrytis cinerea and Colletotrichum gloeosporioides. The Bacillus sp.N311 has good biological prevention and treatment effects in Rape Sclerotinia Rot pot culture prevention and treatment tests, and prevention and treatment effects can reach more than 50%. The Bacillus sp.N311 fermentation supernatant has wide fungus-inhibiting spectrum and good biocontrol effect, has advantages of no toxicity, difficult generation of drug resistance, environment safety and biosafety, and has wide application prospect.

The invention belongs to the technical field of chemical production, and relates to a production process for fermenting an organic fertilizer by using Maotai-flavor liquor vinasse. The production process comprises the following steps: (1) preparing a fermentation inoculant; (2) adding the fermentation inoculant in the step (1) into vinasse and auxiliary materials to prepare a fermentation raw material; and (3) transporting the fermentation raw materials obtained in the step (2) to a fermentation zone, and carrying out compost fermentation to obtain the organic fertilizer, the vinasse being Maotai-flavor liquor vinasse, and the fermentation inoculant being composed of Candida rugosa, Bacillus licheniformis, Paecilomyces variotii and Aspergillus fumigatus. According to the production process, the organic fertilizer production is performed on the Maotai-flavor Baijiu vinasse with characteristics of high acidity, high viscosity and degradation resistance under the conditions of natural pH,natural moisture and no additional chemical material adding, and the obtained organic fertilizer meets the national organic fertilizer standard (NY525-2012) and can be used for more scientifically and more effectively recycling and harmlessly utilizing the spent grains after the production of the Maotai-flavor liquor is finished, and has a wide application prospect in the Maotai-flavor liquor brewing industry.

The invention relates to the technical field of biological strains, particularly a Paecilomyces variotii strain and application thereof. The Paecilomyces variotii strain MM2 is collected to the China General Microbiological Culture Collection Center on July 8th, 2013, and the collection number is CGMCC No.7903. The application method comprises the following steps: adding the strain into any of straw-bran liquid culture medium, filter paper-inorganic salt liquid culture medium or PDA (potato dextrose agar) liquid culture medium, and culturing at 28-30 DEG C for 2-9 days to obtain the single-strain solid microbial inoculant. The Paecilomyces variotii strain can efficiently degrade and utilize cellulose, can be used as an external strain source for recycling lignocellulose solid waste, and can accelerate the degradation of the cellulose; the Paecilomyces variotii strain is separated from wine sediment compost and applied to wine sediment compost, thereby avoiding the problem of difficulty in assessment; and after being added, the microbial inoculant containing the strain effectively promotes the rotting of the wine sediment compost and accelerates the processing of the organic fertilizer.

The invention discloses weight ratio of material components of a compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) A composite medicine and, weight ratio of material components of a compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) B composite medicine, weight ratio of material components of a compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) C composite medicine, a preparation method and steps of the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) A composite medicine, a preparation method and steps of the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) B composite medicine, a preparation method and steps of the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) C composite medicine, as well as the application of the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) A composite medicine, the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) B composite medicine and the compound fermented cordyceps sinensis bacterium powder (paecilomyces hepiali Cs-4 bacterium powder ) C composite medicine for anti-aging, antifatigue, anti-hypoxia, immunity enhanced, sedative, antishock and anti irradiation.

The invention discloses paecilomyces variotii powder as well as a preparation method and an application thereof. The powder is prepared by fermenting paecilomyces variotii with a preservation number of CGMCC No.13772 on a corn flour culture medium. The paecilomyces variotii powder has the beneficial effects that (1) paecilomyces variotii can parasitize nematodes and simultaneously produce multiple secondary metabolites so as to inhibit the growth of the nematodes, so that the paecilomyces variotii powder can be used for effectively preventing and treating the nematodes; (2) the paecilomyces variotii powder is high in spore content and nematode killing efficiency and long in guarantee period; and (3) a preparation process of the paecilomyces variotii powder is simple and suitable for industrial production.

Provided herein are methods of preventing/controlling phytoparasitic nematodes in migratory and sedentary endoparasites belonging to families Anguinidae, Aphelenchidae, Aphelenchoididae, Criconematidae, Dolichodoridae, Hemicycliophoridae, Heteroderidae, Hoplolaimidae, Iotonchidae, Neotylenchidae, Pratylenchidae, Sphaerulariidae, Tilenchidae, and Tylenchulidae: Suborder Tylenchina; Longidoridae: Suborder Dorylaimina; Trichodoridae: Suborder Diphtherophorina using Paecilomyces carneus. The compositions and processes disclosed herein are useful in the prevention and/or control and/or eradication of phytoparasitic nematodes that infect and/or infest the vast majority of cultures for animal and human consumption, while optimum conditions are created in the soil for improving crop yield, with the option of getting organic products.

The invention belongs to the technical field of application of paecilomyces hepialid strains, and discloses a paecilomyces hepialid strain fermentation composition as well as a preparation method and application thereof. SDG in the flaxseed lignan is converted into SECO through the desugaring effect of beta-glucosidase in a culture solution obtained after fermentation of the paecilomyces hepialid strains, the SECO is easier to absorb, and the bioavailability of the flaxseed lignan is promoted to be improved to 75% from 15%; and under the action of [beta]-glucosidase, various glycosides or glycosides in the double-petal red roses, [gamma]-aminobutyric acid, red ginseng, red dates, Chinese wolfberry fruits and cranberries can be converted into aglycones, the aglycones are easily absorbed by a human body, and the bioavailability of effective components in the raw materials is promoted to be remarkably improved. The paecilomyces hepialid strain fermentation composition has the effects of replenishing blood, activating blood, reinforcing qi, nourishing liver, improving metabolism, promoting microcirculation in the body, accelerating internal circulation and conditioning female endocrine dyscrasia, so as to improve the syndrome caused by female estrogen reduction.

The invention provides a paecilomyces lilacinus microcapsule suspending agent. The paecilomyces lilacinus microcapsule suspending agent is prepared from components for forming an oil phase, components for forming a water phase and a capsule wall material, wherein the components for forming the oil phase comprise a paecilomyces lilacinus concentrated bacterial solution and an auxiliary agent; wherein the components for forming the water phase comprise an emulsifier and paecilomyces lilacinus supernatant, the capsule wall material is formed by polymerizing a capsule wall material prepolymer, and the capsule wall material prepolymer comprises isocyanate and organic amine. The auxiliary agent comprises one or more of an acid-base regulator, a dispersing agent, a solvent oil, an anti-freezing agent, a defoaming agent, a preservative and a viscosity regulator; the paecilomyces lilacinus concentrated bacterial solution and the supernatant are obtained by separating paecilomyces lilacinus fermentation bacterial liquid. According to the paecilomyces lilacinus microcapsule suspending agent provided by the invention, the water phase and the oil phase respectively contain metabolite and effective viable bacteria of paecilomyces lilacinus, and compared with conventional powder, granules and water dispersants, the microcapsule suspending agent is longer in shelf life and more stable in performance.

The invention belongs to the technical field of agricultural production and biology, and particularly relates to a penicillium composition with a low-temperature protective effect, a low-temperature protective agent, application of the penicillium composition and the low-temperature protective agent and a method for improving the survival rate of floating seedlings. The invention provides a penicillium composition with a low-temperature protection effect. The penicillium composition comprises penicillium citrinum, penicillium rubrum, penicillium torusii and paecilomyces variotii. The penicillium composition can effectively improve the survival rate and the cold resistance of floating breeding, has the advantages of being low in cost and easy to operate, and has the advantages of being high in safety and free of pollution due to the fact that microorganisms are adopted.

A specific molecular marker of lilacinus pseudopanicillum sp. M-14 strain and its application are disclosed. The randomly amplifying DNA polymorphism technique is used for molecular fingerprint analysis to M-14 strain and the frequently seen fungus in soil to obtain the RAPD specific DNA fragments P850 and P1400 of M-14 strain, which are recovered, purified, cloned and sequenced. The specific SCAR-PCR primer is designed for PCR amplification to the DNA extracted from the soil where M-14 stain is applied and the amplified fragments P850 and P1400 are marked by digoxin to obtain the hybridizing probe for spot hybridization to said extracted DNA, so specifically detecting if there is M-14 strain in natural soil and its quantity.

The invention relates to a rapid detection method for content of adenosine in paecilomyces hepialid mycelium powder. The rapid detection method is characterized by comprising the following steps: 1) preparing adenosine standard solution, namely weighing an adenosine standard substance, dissolving and carrying out constant volume with adenosine extracting solution, and preparing the adenosine standard solution for later use; 2) preparing sample solution, namely weighing a paecilomyces hepialid mycelium powder sample, adding the adenosine extracting solution, dissolving, uniformly mixing, addingquartz sand and grinding to be pasty, cooling, then transferring into a colorimetric tube, carrying out constant volume, carrying out ultrasonic oscillation by virtue of an ultrasonic oscillator, mixing, freezing in an environment at the temperature of -20 DEG C, then melting and transferring into a centrifugal machine to be subjected to centrifugal treatment, taking supernatant, filtering by virtue of a 0.45Mum filtration membrane, and preparing the sample solution for later use; and 3) carrying out detection and analysis by virtue of high performance liquid chromatography. The rapid detection method provided by the invention is scientific and reasonable in design, good in adenosine chromatographic peak separation effect, simple in mobile phase, high in adenosine recovery rate and good in repeatability and has the advantages of easy operation, shorter time, high detection speed and high detection efficiency.

The invention discloses a method for treating the pH of nisin wastewater, which comprises the steps of treating nisin wastewater with a fermentation method, applying Paecilomyces variotii to the treatment of nisin wastewater, cultivating it in the stock solution of nisin wastewater to maintain its vitality, aeration and stirring for 7~ In 9 days, the pH of the nisin wastewater can be neutralized and the COD can be reduced by 60% to 90%. The method for treating the pH of nisin wastewater obtained by the present invention only adds bacteria species and does not add other substances, and is very economical; moreover, the method is simple and easy to handle on a large scale.

The invention discloses a method for solid-state fermentation of cut tobacco stems, and belongs to the technical field of microbial fermentation. According to the method, solid-state fermentation is carried out on cut tobacco stems by using paecilomyces varioti fermentation liquor, and the method mainly comprises the following steps of: (1) slant strain culture; (2) liquid seed culture; (3) culture of paecilomyces varioti fermentation broth; and (4) solid-state fermentation culture, which is implemented by inoculating a cultured paecilomyces varioti fermentation liquid into a solid-state fermentation culture medium, and culturing the paecilomyces varioti fermentation liquid at the temperature of 22-32 DEG C for 6-10 days. The cut tobacco stems are subjected to fermentation and biotransformation of paecilomyces varioti strains, so that cell wall substances in the cut tobacco stems are decomposed and utilized by the paecilomyces species, efficient bioactive substances such as paecilomyces polysaccharide and paecilomyces acid are generated, adverse factors influencing the internal quality and flavor of the cut tobacco stems are reduced, the sensory quality during smoking is improved,and the utilization rate of the tobacco stems in cigarettes is increased.

The invention discloses a preparation method and application of 2'-deoxyguanosine, guanosine and compositions thereof, belonging to the technical field of prevention and control of pathogenic microorganisms, in particular to the inhibition of parahemolysis by 2'-deoxyguanosine and guanosine in marine fish culture The application of vibrio, the application of 2'-deoxyguanosine and guanosine in anti-plant virus, and the application of 2'-deoxyguanosine and guanosine in improving 2'-deoxyguanosine, guanosine, salicylic acid in plants The application in the content; the present invention provides a composition comprising 2'-deoxyguanosine and guanosine, and provides a kind of extracting 2'-deoxyguanosine, guanosine from Paecilomyces variotans SJ1 cells The present invention finds that 2'-deoxyguanosine and guanosine have the effect of preventing and controlling pathogenic microorganisms, especially have obvious anti-plant virus effects, and can improve the 2'-deoxyguanosine, guanosine, saliculus in plants acid content.