158 results about "Plant Immunity" patented technology

The invention discloses a plant immune induced resistance protein PpAE4 and application thereof, and the protein can induce plant resistance and can be used as a plant immune activating factor to be applied to the field of prevention and treatment of plant diseases. The amino acid sequence of the protein is as shown in SEQ ID NO: 2. High-concentration protein can be obtained through expression of the engineering strain, the disease resistance of plants to soybean rust germs can be improved when the protein is used for treating the plants, and the protein is low in needed concentration, quick in effect taking and long in acting time. The PpAE4 provides a new way for improving plant disease resistance and preventing and treating plant diseases.

The invention belongs to the technical field of agricultural science, and particularly provides application of oligosaccharide in prevention and treatment of corn southern leaf blight. The oligosaccharide has an inhibiting effect on the bipolaris maydis and can be used as an inhibitor of the bipolaris maydis. Meanwhile, the oligosaccharide can be used as a plant immune resistance inducer for preventing and treating the corn southern leaf blight, and the occurrence and development of the corn southern leaf blight are prevented and controlled by inducing basic resistance and enhancing the plant immunity. The oligosaccharide application method is simple and convenient to operate, green and environment-friendly, green prevention and control are facilitated, and grain loss caused by diseases is reduced more economically and more environmentally.

The invention provides application of small peptides in preventing and treating plant diseases and promoting plant growth. The structural formula of the small peptide is shown in the specification. Experimental results show that the small peptide shown in the formula A has the effects of improving the disease resistance of plants and regulating the growth of the plants. The small peptide not only can be used as a plant immune resistance inducer at low concentration to improve the immunocompetence of plants for resisting pathogen invasion, but also can promote plant growth, and has a good application prospect. R1-R2-beta-Ala-Trp-Gly-NH2 Formula A

The invention belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsDOF3 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is performed by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and bacterial blight), and it is proved that OsDOF3 participates in regulation and control of an immunoreaction pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (bacterial blight). Meanwhile, the application also finds that after a strong promoter ubi is adopted for driving, an overexpression material of the OsDOF3 is shorter than that of a wild plant, and the OsDOF3 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The invention belongs to the technical field of plant biology, and particularly relates to a gene for regulating and controlling powdery mildew resistance of cucumbers and application of the gene, two cswrky31 mutants are obtained based on a cucumber Tnt1 reverse transcription transposon mutant library, and the two cswrky31 mutants both show high susceptibility to powdery mildew bacteria. A target gene Csa5G551250 which is directly regulated and controlled by CsWRKY31 is screened by virtue of a high-throughput transcriptome sequencing technology and a DNA affinity purification sequencing technology in combination with a yeast one-hybrid experiment. A dual luciferase report experiment shows that the CsWRKY31 positively regulates and controls the expression of the Csa5G551250, and the overexpression of the Csa5G551250 gene promotes the resistance of the cucumber to powdery mildew bacteria. A new reference gene resource is provided for cultivation of cucumber disease-resistant varieties, and a new thought is provided for molecular mechanism research of transcription factor mediated plant immunity.

The invention belongs to the technical field of agricultural biology, and particularly discloses an RcPR5 gene for regulating and controlling gray mold resistance of Chinese roses and application of the RcPR5 gene, and the nucleotide sequence of the RcPR5 gene is shown as SEQ ID NO.1. The RcPR5 gene provided by the invention is remarkably associated with the gray mold resistance, plays an important role in plant immunity, regulates and controls immunity through an RNA processing complex, and has the advantages that the resistance of Chinese roses to gray mold is regulated and controlled; transcriptome difference analysis shows that the expression quantity of the RcPR5 is induced by grey mould to be remarkably up-regulated, after the RcPR5 gene is silenced, the petal scab is remarkably increased, it is proved that the RcPR5 gene really plays a role in the disease resistance process of the gray mold of the Chinese rose, breeding of the gray mold resistant Chinese rose can be conducted through the gene by adopting the modern gene engineering technology, and the breeding cost is lowered. And new germplasm is developed and introduced.

The present invention belongs to the field of biotechnology. Disclosed are a plant immunity-inducing protein CcHE1 and a use thereof. The disclosed plant immunity-inducing protein CcHE1 secreted by a plant pathogenic fungus has an amino acid sequence shown as SEQ ID NO.2, and a nucleotide sequence of the gene encoding CcHE1 shown as SEQ ID NO.1. The present invention provides a novel plant immunity-inducing protein CcHE1, capable of inducing plant resistance and immune defense responses, significantly enhancing plant disease resistance, effective at low concentrations, and exhibiting remarkable disease-resistance effects. CcHE1 activates the plant's own immune system, providing a new approach to improving plant resistance, and has broad application prospects in areas such as green control of forest diseases.

The invention relates to three antibacterial peptides and application thereof in resisting fungi and / or inducing plant immunity. Specifically, on one hand, the invention provides three antibacterial peptides which are novel functional antibacterial peptides ZJUAMP1, ZJUAMP2 and ZJUAMP65, the sequences of the novel functional antibacterial peptides ZJUAMP1, ZJUAMP2 and ZJUAMP65 are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, and the novel functional antibacterial peptides have good antifungal activity, can activate an MAPK signal channel in a plant body and induce the plant to generate systematic disease resistance, and are suitable for the fields of crop disease prevention and control and plant protection. The two antibacterial peptides are simple in structure and easy to synthesize. On the other hand, the invention further provides application of the two antibacterial peptides in resisting fungi, activating a plant MAPK signal channel, inducing plant immune response, enhancing plant disease resistance and / or preventing and treating plant diseases.

The invention provides a small molecule compound capable of inducing plant immune response and improving plant disease resistance. Specifically, it is found for the first time that the 2'c ADPR shown in the formula I or the analogue thereof, or the pharmaceutically acceptable salt thereof can significantly enhance the resistance of plants to pathogenic microorganisms, diseases and pests and viruses.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an application of SlERF098 protein and a coding gene thereof in regulating and controlling steroid alkaloid content to influence botrytis cinerea resistance. According to the application, the resistance of tomatoes to gray mold is enhanced by regulating and controlling the metabolism of steroid glycoalkaloid through the coding gene. The tomato SlERF098 gene is positioned through whole genome association analysis, and an overexpression strain and an RNA interference strain of the tomato SlERF098 gene are constructed. Experiments prove that the SlERF098 can obviously activate the expression of the GAMEs gene in the SGAs metabolic pathway. In addition, overexpression of the SlERF098 gene can improve the content of SGAs metabolites in tomatoes and the resistance to gray mold, while RNA interference strains present an opposite trend. The invention provides a new target for research on disease-resistant molecular breeding and plant immunity-metabolism coordinated regulation and control, and has important agricultural application value.

The short peptide NP19, polypeptide Nlsp5 provided by the present invention and their applications belong to the technical field of bioengineering. The short peptide NP19 and polypeptide Nlsp5 can significantly activate the plant immune system, improve the insect resistance of plants, enhance the ability of plants to resist insects, and effectively prevent or reduce the occurrence of insect pests. The short peptide NP19 and polypeptide Nlsp5 of the present invention provide a new way for improving the insect resistance of plants, provide resources for the development and application of future biological pesticides, can be used as a new type of microbial protein pesticide to defend against insect pest attacks, and also provide a basis for future transgenic plants of this gene, having broad application prospects in agricultural production.

The invention discloses a protein capable of inducing a plant immune activation function and application of the protein in disease control, the gene sequence of the protein capable of inducing the plant immune activation function is shown as SEQ ID NO: 1, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. According to the invention, a novel plant immune activator protein CgSsp16 secreted by colletotrichum gloeosporioides is identified, can be identified by plants, stimulates a plant immune response mechanism, triggers broad-spectrum resistance of host plants to various pathogens and improves disease resistance of various plants; the compound is expected to be developed into a novel biopesticide to be applied to green and sustainable production of agriculture.

The application belongs to the technical field of genetic engineering, and relates to application of an immune negative regulatory factor NbELP1 in improving plant disease resistance. NbELP1 The gene is used as a plant immune negative regulatory factor, and negatively regulates early immune response induced by PAMP. NbELP1 Knocking out the gene can effectively improve the disease resistance of plants in response to pathogenic bacteria infection, and does not affect the normal growth of plants. NbELP1 The immune negative regulatory factor gene or its orthologous gene widely exists in various plants and is functionally conservative, is a high-quality gene resource with potential for genetic improvement of disease resistance of various plants, and has great application prospect.

The invention discloses a fertilizer for promoting and improving the immunity of plants and a preparation method, and relates to the field of fertilizers. The preparation method comprises the following steps: mixing, smashing and grinding earthworms, eucalyptus leaves, wheat leaves and hawthorn leaves, putting into an ethanol solution, oscillating for 20-24 hours in a shaking table, and centrifugally filtering to obtain an active extracting solution; adding a sodium alginate solution into the suspended bacteria solution and the active extracting solution, and slowly adding carboxymethyl chitosan and a calcium chloride solution; and preparing. According to the invention, the plant growth-promoting rhizobacteria are fixed in the composite hydrogel, and the composite hydrogel can promote colonization of strains at plant root tips and lateral roots so as to regulate deposition of endothelial cell suberite, optimize mineral nutrition balance and abiotic stress response of plants and improve accumulation of auxin at the root tips; meanwhile, an active extracting solution is added into the composite hydrogel, immune response can be activated, and the metabolic activity and antioxidant activity of plants are greatly improved in combination with extracts of other substances.

The invention provides an application of a GhIQD1 gene in plant verticillium wilt resistance, and particularly provides an application of the GhIQD1 gene in regulation and control of plant verticillium wilt resistance. When the expression quantity of the GhIQD1 gene in the plant is relatively high, the verticillium wilt resistance of the plant is enhanced, and when the expression quantity of the GhIQD1 gene in the plant is relatively low or the GhIQD1 gene is not expressed, the verticillium wilt resistance of the plant is weakened. The new research finds that not only is the understanding of the plant immune system enriched, but also important guidance is provided for developing more effective disease-resistant measures and strategies.

The application provides a polypeptide, a plant immune elicitor and application thereof. The polypeptide has 10 or 11 amino acid residues; the amino acid at the first position of the polypeptide is serine, and the amino acids at the 9th and 10th positions are both cysteine; if the polypeptide has 11 amino acid residues, the amino acid at the 11th position of the polypeptide is asparagine. The polypeptide can solve the problem that there is no small peptide secreted by gramineae plants in the prior art, and is suitable for the field of plant immune elicitors.

The invention provides application of beta-1, 4-glucan type cellooligosaccharide in improvement of plant rhizosphere micro-ecology and enhancement of IAA signals, and belongs to the technical field of biological agriculture. The invention provides an application of beta-1, 4-glucan type cellooligosaccharide with the polymerization degree of DP2-DP5 in improvement of plant rhizosphere micro-ecology and enhancement of IAA (Indole Acetic Acid) signals. In the invention, beta-1, 4-glucan type cellooligosaccharide can induce plant immunity in a growth allowable PTI threshold range, so that a rhizosphere microflora structure is directionally remodeled, the relative abundance of beneficial microorganisms is improved, the potential pathogen load is reduced, rhizosphere IAA and related metabolites thereof can be increased, and'disease resistance without growth inhibition 'is realized. The invention further provides an application of the beta-1, 4-glucan type cellooligosaccharide combined with rhizosphere beneficial microorganisms in biological agriculture, and growth promotion and / or nodulation nitrogen fixation effects can be enhanced.

The invention belongs to the technical field of gene editing, and particularly relates to a WER10 enhancer and application thereof. The invention provides application of a WER10 nucleic acid molecule and / or a biological material for regulating and controlling the WER10 nucleic acid molecule, the nucleotide sequence of the WER10 nucleic acid molecule is as shown in SEQ ID NO: 1, and the application comprises any one or more of the following items: 1) application of the sequence as shown in SEQ ID NO: 1 as an enhancer; 2) application in enhancing the expression of a downstream reporter gene LUC induced by an immune induction signal; and 3) application in regulation and control of the broad-spectrum antibacterial property of the plants. The sequence and the characteristics of the pathogenic bacterium induced enhancer WER10 located in the intergene region are identified and verified from arabidopsis thaliana, plant immunity related cis-regulatory element resources are enriched, and a new resource is provided for constructing a pathogen induced disease-resistant expression system and developing crop disease-resistant molecular design and breeding.

The invention provides a polypeptide, a plant immune resistance inducer and application of the plant immune resistance inducer. The polypeptide has 10 or 11 amino acid residues; the first amino acid of the polypeptide is serine, and the ninth amino acid and the tenth amino acid of the polypeptide are cysteine; if the polypeptide has 11 amino acid residues, the 11th amino acid of the polypeptide is asparagine. The method can solve the problem of lack of small peptides secreted by gramineous plants in the prior art, and is suitable for the field of plant immune resistance inducers.

The application discloses a plant immunity elicitor protein PpAE4 and application thereof, the protein can induce plant resistance, and can be applied to the field of plant disease prevention and treatment as a plant immunity activator. The amino acid sequence of the protein is shown as SEQ ID NO:2. High-concentration protein can be obtained by using an engineering strain for expression, and the protein can be used for treating plants to improve the disease resistance of the plants to soybean rust fungi, and the required concentration is low, the effect is quick, and the action time is long. The PpAE4 provides a new way for improving the disease resistance of plants and preventing and treating plant diseases.

The application belongs to the field of plant biotechnology, and particularly relates to a cucumber CsODO1 gene and application thereof, and is a MYB transcription factor capable of being combined with a CsCSE1 promoter, which is screened through a yeast single hybridization. It is proved through EMSA and tobacco transient expression analysis that the CsODO1 gene directly and specifically combines with a "CAACCA" sequence in the CsCSE1 promoter, and inhibits the expression of the CsCSE1 gene. The CsODO1 gene can be induced to express by a powdery mildew fungus, and is differentially expressed in the cucumber infected by the powdery mildew fungus. It is found through a cucumber cotyledon transient transformation system mediated by an agrobacterium that the CsODO1 gene negatively regulates the biosynthesis of lignin and the resistance of the cucumber to the powdery mildew fungus. The application provides a new reference gene resource for the cultivation of a cucumber disease-resistant variety, and provides a new thought for the research on the molecular mechanism of the plant immunity mediated by the lignin pathway.

The invention discloses application of pseudopenicillin variotii in preparation of a resistance inducer for preventing and treating plant diseases, and belongs to the technical field of plant protection. The invention provides an application of Panax variotii in prevention and treatment of plant diseases, the application concentration of the Panax variotii is 0.1 microgram / mL-1 microgram / mL, a plant resistance inducer containing the Panax variotii can be sprayed on plant leaves or fruit surfaces and has a remarkable prevention effect on anthrax leaf blight, and the higher the concentration is, the better the prevention effect is; the pseudopenicillin variotii can promote synthesis or activity improvement of catalase, peroxidase, polyphenol oxidase, glutathione S-transferase and superoxide dismutase in apple bodies, and also can remarkably induce up-regulation expression of disease-resistant related genes such as MdPR2 and MdPR5, so that the disease resistance, the antioxidant level and the plant immunity of apples are enhanced; a novel and efficient plant resistance inducer application scheme is provided for apple disease control and physiological regulation.

Provided in the present disclosure are a short peptide NP19, a polypeptide Nlsp5 and application thereof, which belong to the field of bioengineering technology. The short peptide NP19 and the polypeptide Nlsp5 can significantly activate the plant immune system, improve the insect resistance of plants, enhance the ability of plants to resist insects, and effectively prevent or reduce the occurrence of insect pests. The short peptide NP19 and the polypeptide Nlsp5 of the present disclosure provide a new way to improve the insect resistance of plants, provide resources for the future development and application of biopesticides, can be used as a new type of microbial protein pesticide to defend against insect pests, and also provide a basis for the future transgenic plants of this gene, thus having broad application prospects in agricultural production.

The invention provides a composition for preventing and treating plant diseases and application thereof. The composition comprises antibacterial lipopeptide and a protein plant immunity inducer. Synchronous and efficient prevention and control of fungal black shank and viral PVY and CMV diseases are achieved for the first time, the prevention effect reaches 88% or above, and the industrial problem that the prevention and control spectrum of a single agent is narrow is solved.

The present invention belongs to the field of molecular biology technology, and specifically relates to a plant immune-inducing protein EqBPIE1 and its application. The amino acid sequence of the protein EqBPIE1 is shown in SEQ ID No. 1. The protein EqBPIE1 provided by the present invention can enhance the plant's own immune system from the plant itself, thereby enabling the plant to cope with more diverse environmental changes and infection by multiple pathogens; it has an inhibitory effect on the infection of multiple pathogens on plants, covering multiple pathogens such as rubber tree powdery mildew, Siamese anthracnose, gray mold, Pseudomonas syringae, pepper phytophthora, and soybean phytophthora, and can significantly improve plant disease resistance. The antibacterial effect can be produced at a concentration of 0.1 to 1 μM.

The invention discloses an application of FGSG09291 protein and a coding gene thereof in improving plant disease resistance, and belongs to the technical field of plant immune induced resistance. According to the invention, a novel FGSG09291 protein with a plant immune activation function is found from fusarium graminearum, and the FGSG09291 protein can promote plant active oxygen outbreak and callose accumulation and provide basic immune response for plants; in addition, high expression of the PR1a gene, the WRKY70 gene and the LOX3 gene can be induced, the PR1a gene and the WRKY70 gene are genes of a salicylic acid pathway, and the increase of salicylic acid can improve the broad-spectrum resistance of plants. Experiments prove that the prevention and control effect of corn on dwarf mosaic disease can be enhanced by externally applying the FGSG09291 protein.

The invention belongs to the field of molecular biology, and particularly relates to application of OsPLDRP1 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsPLDRP1 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is carried out by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and xanthomonas oryzae), and it is proved that OsPLDRP1 participates in regulation and control of immunoreaction pathways of rice to fungal diseases (rice blast and banded sclerotial blight) and bacterial diseases (xanthomonas oryzae). Meanwhile, it is found that the ear length of a mutant material of the OsPLDRP1 is shorter than that of a wild type, after driving is conducted through a strong promoter ubi, the plant type of an overexpression material of the OsPLDRP1 is looser than that of the wild type, the plant becomes higher, the heading phenotype is early, and the OsPLDRP1 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

This invention discloses a method and its application for silencing plant disease-susceptible genes by loading double-stranded RNA (dsRNA) onto a three-dimensional mesoporous carrier of nanolayered carbon microcrystals, belonging to the field of nanopesticide formulation technology. After amino-functionalization modification, the carrier covalently couples with terminally carboxylated and 2′-O-methyl-modified dsRNA, achieving highly efficient loading (loading rate > 95%) through synergistic effects of π-π stacking, significantly improving the stability, leaf permeability, and resistance to nuclease degradation of the dsRNA. After delivering the dsRNA targeting the disease-susceptible gene to plant cells, it can specifically activate RNA interference, efficiently silencing the disease-susceptible gene and enhancing plant immunity. Further encapsulation in biodegradable polymer microcapsules can form a long-acting formulation with sustained-release properties. This invention provides an efficient, stable, and environmentally friendly RNA delivery strategy for plant disease control.

The invention discloses application of trans, cis-2, 6-nonadienal in prevention and treatment of rice diseases, and belongs to the technical field of plant disease prevention and treatment. Research finds that the trans, cis-2, 6-nonadienal as a plant self-synthesized substance has an obvious inhibition effect on the growth of xanthomonas oryzae and pyricularia grisea, and the trans, cis-2, 6-nonadienal can be used as an effective component to be applied to prevention and treatment of field bacterial streak and rice blast and improve plant immunity. The invention provides theoretical support for developing novel pesticides for preventing and treating rice bacterial streak and rice blast, and has important significance.