85 results about "Plant Immunity" patented technology

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The present invention belongs to the field of biotechnology. Disclosed are a plant immunity-inducing protein CcHE1 and a use thereof. The disclosed plant immunity-inducing protein CcHE1 secreted by a plant pathogenic fungus has an amino acid sequence shown as SEQ ID NO.2, and a nucleotide sequence of the gene encoding CcHE1 shown as SEQ ID NO.1. The present invention provides a novel plant immunity-inducing protein CcHE1, capable of inducing plant resistance and immune defense responses, significantly enhancing plant disease resistance, effective at low concentrations, and exhibiting remarkable disease-resistance effects. CcHE1 activates the plant's own immune system, providing a new approach to improving plant resistance, and has broad application prospects in areas such as green control of forest diseases.

The invention relates to three antibacterial peptides and application thereof in resisting fungi and / or inducing plant immunity. Specifically, on one hand, the invention provides three antibacterial peptides which are novel functional antibacterial peptides ZJUAMP1, ZJUAMP2 and ZJUAMP65, the sequences of the novel functional antibacterial peptides ZJUAMP1, ZJUAMP2 and ZJUAMP65 are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, and the novel functional antibacterial peptides have good antifungal activity, can activate an MAPK signal channel in a plant body and induce the plant to generate systematic disease resistance, and are suitable for the fields of crop disease prevention and control and plant protection. The two antibacterial peptides are simple in structure and easy to synthesize. On the other hand, the invention further provides application of the two antibacterial peptides in resisting fungi, activating a plant MAPK signal channel, inducing plant immune response, enhancing plant disease resistance and / or preventing and treating plant diseases.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an application of SlERF098 protein and a coding gene thereof in regulating and controlling steroid alkaloid content to influence botrytis cinerea resistance. According to the application, the resistance of tomatoes to gray mold is enhanced by regulating and controlling the metabolism of steroid glycoalkaloid through the coding gene. The tomato SlERF098 gene is positioned through whole genome association analysis, and an overexpression strain and an RNA interference strain of the tomato SlERF098 gene are constructed. Experiments prove that the SlERF098 can obviously activate the expression of the GAMEs gene in the SGAs metabolic pathway. In addition, overexpression of the SlERF098 gene can improve the content of SGAs metabolites in tomatoes and the resistance to gray mold, while RNA interference strains present an opposite trend. The invention provides a new target for research on disease-resistant molecular breeding and plant immunity-metabolism coordinated regulation and control, and has important agricultural application value.

The invention discloses a protein capable of inducing a plant immune activation function and application of the protein in disease control, the gene sequence of the protein capable of inducing the plant immune activation function is shown as SEQ ID NO: 1, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. According to the invention, a novel plant immune activator protein CgSsp16 secreted by colletotrichum gloeosporioides is identified, can be identified by plants, stimulates a plant immune response mechanism, triggers broad-spectrum resistance of host plants to various pathogens and improves disease resistance of various plants; the compound is expected to be developed into a novel biopesticide to be applied to green and sustainable production of agriculture.

The application belongs to the technical field of genetic engineering, and relates to application of an immune negative regulatory factor NbELP1 in improving plant disease resistance. NbELP1 The gene is used as a plant immune negative regulatory factor, and negatively regulates early immune response induced by PAMP. NbELP1 Knocking out the gene can effectively improve the disease resistance of plants in response to pathogenic bacteria infection, and does not affect the normal growth of plants. NbELP1 The immune negative regulatory factor gene or its orthologous gene widely exists in various plants and is functionally conservative, is a high-quality gene resource with potential for genetic improvement of disease resistance of various plants, and has great application prospect.

The invention discloses a fertilizer for promoting and improving the immunity of plants and a preparation method, and relates to the field of fertilizers. The preparation method comprises the following steps: mixing, smashing and grinding earthworms, eucalyptus leaves, wheat leaves and hawthorn leaves, putting into an ethanol solution, oscillating for 20-24 hours in a shaking table, and centrifugally filtering to obtain an active extracting solution; adding a sodium alginate solution into the suspended bacteria solution and the active extracting solution, and slowly adding carboxymethyl chitosan and a calcium chloride solution; and preparing. According to the invention, the plant growth-promoting rhizobacteria are fixed in the composite hydrogel, and the composite hydrogel can promote colonization of strains at plant root tips and lateral roots so as to regulate deposition of endothelial cell suberite, optimize mineral nutrition balance and abiotic stress response of plants and improve accumulation of auxin at the root tips; meanwhile, an active extracting solution is added into the composite hydrogel, immune response can be activated, and the metabolic activity and antioxidant activity of plants are greatly improved in combination with extracts of other substances.

The application provides a polypeptide, a plant immune elicitor and application thereof. The polypeptide has 10 or 11 amino acid residues; the amino acid at the first position of the polypeptide is serine, and the amino acids at the 9th and 10th positions are both cysteine; if the polypeptide has 11 amino acid residues, the amino acid at the 11th position of the polypeptide is asparagine. The polypeptide can solve the problem that there is no small peptide secreted by gramineae plants in the prior art, and is suitable for the field of plant immune elicitors.

The invention provides application of beta-1, 4-glucan type cellooligosaccharide in improvement of plant rhizosphere micro-ecology and enhancement of IAA signals, and belongs to the technical field of biological agriculture. The invention provides an application of beta-1, 4-glucan type cellooligosaccharide with the polymerization degree of DP2-DP5 in improvement of plant rhizosphere micro-ecology and enhancement of IAA (Indole Acetic Acid) signals. In the invention, beta-1, 4-glucan type cellooligosaccharide can induce plant immunity in a growth allowable PTI threshold range, so that a rhizosphere microflora structure is directionally remodeled, the relative abundance of beneficial microorganisms is improved, the potential pathogen load is reduced, rhizosphere IAA and related metabolites thereof can be increased, and'disease resistance without growth inhibition 'is realized. The invention further provides an application of the beta-1, 4-glucan type cellooligosaccharide combined with rhizosphere beneficial microorganisms in biological agriculture, and growth promotion and / or nodulation nitrogen fixation effects can be enhanced.

The invention belongs to the technical field of gene editing, and particularly relates to a WER10 enhancer and application thereof. The invention provides application of a WER10 nucleic acid molecule and / or a biological material for regulating and controlling the WER10 nucleic acid molecule, the nucleotide sequence of the WER10 nucleic acid molecule is as shown in SEQ ID NO: 1, and the application comprises any one or more of the following items: 1) application of the sequence as shown in SEQ ID NO: 1 as an enhancer; 2) application in enhancing the expression of a downstream reporter gene LUC induced by an immune induction signal; and 3) application in regulation and control of the broad-spectrum antibacterial property of the plants. The sequence and the characteristics of the pathogenic bacterium induced enhancer WER10 located in the intergene region are identified and verified from arabidopsis thaliana, plant immunity related cis-regulatory element resources are enriched, and a new resource is provided for constructing a pathogen induced disease-resistant expression system and developing crop disease-resistant molecular design and breeding.

The application discloses a plant immunity elicitor protein PpAE4 and application thereof, the protein can induce plant resistance, and can be applied to the field of plant disease prevention and treatment as a plant immunity activator. The amino acid sequence of the protein is shown as SEQ ID NO:2. High-concentration protein can be obtained by using an engineering strain for expression, and the protein can be used for treating plants to improve the disease resistance of the plants to soybean rust fungi, and the required concentration is low, the effect is quick, and the action time is long. The PpAE4 provides a new way for improving the disease resistance of plants and preventing and treating plant diseases.

The application belongs to the field of plant biotechnology, and particularly relates to a cucumber CsODO1 gene and application thereof, and is a MYB transcription factor capable of being combined with a CsCSE1 promoter, which is screened through a yeast single hybridization. It is proved through EMSA and tobacco transient expression analysis that the CsODO1 gene directly and specifically combines with a "CAACCA" sequence in the CsCSE1 promoter, and inhibits the expression of the CsCSE1 gene. The CsODO1 gene can be induced to express by a powdery mildew fungus, and is differentially expressed in the cucumber infected by the powdery mildew fungus. It is found through a cucumber cotyledon transient transformation system mediated by an agrobacterium that the CsODO1 gene negatively regulates the biosynthesis of lignin and the resistance of the cucumber to the powdery mildew fungus. The application provides a new reference gene resource for the cultivation of a cucumber disease-resistant variety, and provides a new thought for the research on the molecular mechanism of the plant immunity mediated by the lignin pathway.

The invention discloses application of pseudopenicillin variotii in preparation of a resistance inducer for preventing and treating plant diseases, and belongs to the technical field of plant protection. The invention provides an application of Panax variotii in prevention and treatment of plant diseases, the application concentration of the Panax variotii is 0.1 microgram / mL-1 microgram / mL, a plant resistance inducer containing the Panax variotii can be sprayed on plant leaves or fruit surfaces and has a remarkable prevention effect on anthrax leaf blight, and the higher the concentration is, the better the prevention effect is; the pseudopenicillin variotii can promote synthesis or activity improvement of catalase, peroxidase, polyphenol oxidase, glutathione S-transferase and superoxide dismutase in apple bodies, and also can remarkably induce up-regulation expression of disease-resistant related genes such as MdPR2 and MdPR5, so that the disease resistance, the antioxidant level and the plant immunity of apples are enhanced; a novel and efficient plant resistance inducer application scheme is provided for apple disease control and physiological regulation.

Provided in the present disclosure are a short peptide NP19, a polypeptide Nlsp5 and application thereof, which belong to the field of bioengineering technology. The short peptide NP19 and the polypeptide Nlsp5 can significantly activate the plant immune system, improve the insect resistance of plants, enhance the ability of plants to resist insects, and effectively prevent or reduce the occurrence of insect pests. The short peptide NP19 and the polypeptide Nlsp5 of the present disclosure provide a new way to improve the insect resistance of plants, provide resources for the future development and application of biopesticides, can be used as a new type of microbial protein pesticide to defend against insect pests, and also provide a basis for the future transgenic plants of this gene, thus having broad application prospects in agricultural production.

The invention provides a composition for preventing and treating plant diseases and application thereof. The composition comprises antibacterial lipopeptide and a protein plant immunity inducer. Synchronous and efficient prevention and control of fungal black shank and viral PVY and CMV diseases are achieved for the first time, the prevention effect reaches 88% or above, and the industrial problem that the prevention and control spectrum of a single agent is narrow is solved.

The invention discloses an application of FGSG09291 protein and a coding gene thereof in improving plant disease resistance, and belongs to the technical field of plant immune induced resistance. According to the invention, a novel FGSG09291 protein with a plant immune activation function is found from fusarium graminearum, and the FGSG09291 protein can promote plant active oxygen outbreak and callose accumulation and provide basic immune response for plants; in addition, high expression of the PR1a gene, the WRKY70 gene and the LOX3 gene can be induced, the PR1a gene and the WRKY70 gene are genes of a salicylic acid pathway, and the increase of salicylic acid can improve the broad-spectrum resistance of plants. Experiments prove that the prevention and control effect of corn on dwarf mosaic disease can be enhanced by externally applying the FGSG09291 protein.

This invention discloses a method and its application for silencing plant disease-susceptible genes by loading double-stranded RNA (dsRNA) onto a three-dimensional mesoporous carrier of nanolayered carbon microcrystals, belonging to the field of nanopesticide formulation technology. After amino-functionalization modification, the carrier covalently couples with terminally carboxylated and 2′-O-methyl-modified dsRNA, achieving highly efficient loading (loading rate > 95%) through synergistic effects of π-π stacking, significantly improving the stability, leaf permeability, and resistance to nuclease degradation of the dsRNA. After delivering the dsRNA targeting the disease-susceptible gene to plant cells, it can specifically activate RNA interference, efficiently silencing the disease-susceptible gene and enhancing plant immunity. Further encapsulation in biodegradable polymer microcapsules can form a long-acting formulation with sustained-release properties. This invention provides an efficient, stable, and environmentally friendly RNA delivery strategy for plant disease control.

This invention discloses the application of Panax notoginseng saponin Ft1 as a plant immune inducer in the control of bacterial plant diseases, belonging to the field of biopesticide technology. This invention is the first to discover that Panax notoginseng saponin Ft1 can effectively activate the plant immune system, promote the expression of the FRK1 immune marker gene, induce reactive oxygen species bursts and calcium ion influx, and activate MAPK protein phosphorylation, thereby enhancing plant resistance to bacterial diseases such as *Pseudomonas syringae*. Unlike other existing Panax notoginseng saponins that directly inhibit fungi as fungicides, Panax notoginseng saponin Ft1 works by activating the plant's own immunity and has no inhibitory effect on plant growth at effective concentrations. It is highly efficient, safe, and environmentally friendly, and can be used as a novel biopesticide for the control of bacterial plant diseases.

The invention discloses an SONAR gene for regulating and controlling heat resistance, nodulation and immunity of plants and application, and belongs to the field of gene engineering. The invention provides an SONAR gene of which the nucleotide sequence is shown as SEQ ID NO.1, and a soybean plant of which the SONAR gene is over-expressed and knocked out and an arabidopsis thaliana plant of which the SONAR gene is over-expressed are respectively constructed. Experimental results show that overexpression of the SONAR gene can significantly improve the tolerance of soybean and arabidopsis thaliana to 40 DEG C high temperature stress, and under the condition of no pathogen, the basic expression level and transcription response amplitude of immune related genes are effectively improved, and plant immune pre-excitation is induced. The knockout of the SONAR gene can significantly increase the nodulation number of soybean plants. The invention provides a new regulation target for the heat resistance, nodulation quantity and immune pre-excitation of plants, and provides a biological material for the cultivation of new soybean germplasm.

The application discloses a tomato Soly04g076730 Gene or its encoded protein is applied to resisting plant geminivirus. Soly04g076730 The gene is transiently expressed or stably genetically transformed in a plant leaf. When a virus invades the plant, the plant immune response is rapidly induced, and the disease resistance of the plant is improved. The Soly04g076730 protein is a secretory protein. The plant immune ability can be improved by expressing or externally applying the protein in the plant, and the virus transmission and replication can be effectively inhibited.

The application discloses application of an immune elicitor protein FvXynC and a protein combination in plant disease resistance, and belongs to the technical field of plant immune elicitors. A new immune elicitor protein FvXynC is found from F. pseudograminearum, and the immune elicitor protein has the core functions of activating plant immune response and improving plant resistance to F. pseudograminearum, can be used as an efficient plant immune inducer, provides a new technical path for plant disease resistance improvement and green plant disease control, and has important agricultural application value. The FvXynC and the immune elicitor protein FvGH43A are compounded, so that the resistance level of corn to F. pseudograminearum can be synergistically improved, and the problem that the plant disease control effect of a single immune elicitor protein is limited is solved.

The invention discloses application of zymosan in promoting plant growth and resisting diseases, and belongs to the field of agriculture. The invention provides an application of zymosan in promoting plant growth, wherein the use concentration is 300-800 ppm. After the zymosan is applied, the plant height growth rate of the plant is increased by 10%-25%, and the root system number, the root system length or the leaf area are remarkably increased. The invention also provides a plant growth promoting composition containing the zymosan. The zymosan also has the activity of inducing plant immune resistance. The zymosan disclosed by the invention has dual functions of promoting growth and resisting diseases, is environment-friendly and high in safety, and has a wide application prospect in green agriculture and sustainable agriculture.

The application provides a kind of 3, 4-dichloroisothiazole benzamide derivatives and preparation method and purposes thereof, specifically relates to a kind of derivatives, and the chemical structure general formula is seen formula I: the application discloses the general formula of the structure of the above compound, synthesis method and as insecticide, acaricide, fungicide, anti-plant virus agent, plant activator purposes, it is combined with agricultural acceptable adjuvant or synergist and with commercial insecticide, acaricide, fungicide, anti-plant virus agent, plant immune activator in the prevention and treatment of agricultural, forestry, horticultural plant pest, mite damage, disease, viral disease purposes and preparation method.

The invention discloses a glomus huanghuai CRN effect protein as well as a coding gene and application thereof, the glomus huanghuai CRN effect protein is CRN4, and the amino acid sequence of the glomus huanghuai CRN effect protein is shown as SEQ ID NO.2. The invention further discloses a preparation method of the glomus huanghuai CRN effect protein. According to the invention, a gene CRN4 which is derived from glomus huanghuai and can trigger a plant anaphylactic reaction (HR) is obtained through a large amount of screening. The protein comprises a conservative LXLFLAK structural domain, and is a typical CRN effect protein. HR reaction can be stimulated, plant immunity is triggered, plants are induced to generate disease-resistant reaction, and great significance is achieved for developing environment-friendly disease-resistant strategies.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat VOZ transcription factor or a coding gene thereof in regulation and control of stripe rust resistance of crops. The invention systematically reveals that wheat transcription factors TaVOZ1 and TaVOZ2 play a positive regulation role in immune response of wheat to stripe rust for the first time, and enriches a functional spectrum of a VOZ family in plant immune regulation; meanwhile, the broad-spectrum resistance of an overexpression material to wheat stripe rust (Pst) is remarkably enhanced by overexpressing the encoding gene of the wheat VOZ transcription factor in wheat, and the wheat VOZ transcription factor has practical breeding application potential, so that the wheat VOZ transcription factor encoding gene provided by the invention can be used as an important candidate for breeding disease-resistant molecules; a new way and a new strategy are provided for green prevention and control of wheat diseases.

The invention relates to the technical field of agricultural biotechnology and plant protection, and discloses application of 2, 4-di-tert-butylphenol nanoemulsion as a plant immune resistance inducer, the 2, 4-di-tert-butylphenol nanoemulsion comprises the following components: 2, 4-di-tert-butylphenol, ethyl acetate, Tween 80 and water, the 2, 4-di-tert-butylphenol, ethyl acetate, Tween 80 and water are added into the 2, 4-di-tert-butylphenol nanoemulsion for the first time, and the 2, 4-di-tert-butylphenol nanoemulsion is added into the plant immune resistance inducer According to the present invention, 2, 4-di-tert-butylphenol is developed into the plant immune resistance inducer, the problems of poor water solubility and low bioavailability of the compound are solved by using the nano-emulsion technology, the brand new application field of the compound is expanded, and the nano-emulsion belongs to a thermodynamic stable system, is not easily layered or precipitated after long-term storage, and has good storage and transportation stability.

This invention belongs to the field of bio-agricultural technology, specifically relating to genes. DISC1 The application of its encoded proteins in regulating plant resistance to Phytophthora parasiticans. This invention, through the study of... DISC1 Studies on the function of genes and their encoded proteins have revealed that... DISC1 Negative regulation of plant resistance to Phytophthora parasiticum. Overexpressed genes. DISC1 Significantly reduces plant resistance to Phytophthora parasiticum, gene DISC1 The mutant plants exhibited significantly enhanced resistance to Phytophthora parasiticum. Further research into the immune mechanism revealed that the gene... DISC1 This invention regulates plant immune responses by modulating genes related to the jasmonic acid (JA) signaling pathway. It provides a novel gene resource for the control of plant resistance to Phytophthora parasiticum.

The invention provides an application of sclerotinia sclerotiorum small peptide Xyn25 in improvement of plant disease resistance, and belongs to the technical field of biopesticides. The invention provides a sclerotinia sclerotiorum small peptide Xyn25. The amino acid sequence of the sclerotinia sclerotiorum small peptide Xyn25 is shown as SEQ ID NO: 1. The sclerotinia sclerotiorum small peptide Xyn25 can efficiently activate plant immune response and improve the disease resistance of plants. The embodiment of the invention shows that the sclerotinia sclerotiorum small peptide Xyn25 can improve the resistance of wheat to gibberellic disease and rice to banded sclerotial blight. On the basis, the invention further provides an immune resistance inducer which comprises the sclerotinia sclerotiorum small peptide Xyn25 and auxiliary materials. The immune resistance inducer provided by the invention meets the development requirements of pesticide reduction, quality improvement and efficiency improvement in green agriculture, and has a good application prospect in the aspects of reducing the dependency degree of chemical pesticides and the like.

The invention discloses a plant immune induced resistance protein Ps131903 and application thereof. The amino acid sequence of the immune induced resistance protein Ps131903 is shown as SEQ ID NO.2. The protein Ps131903 can reduce the harm of phytophthora sojae and pythium ultimum to soybeans, reduce the harm of phytophthora capsici to chilies and tomatoes and reduce the harm of pyricularia grisea to rice. The compound has the advantages of low use concentration, quick effect and broad spectrum, and provides a new way for improving the plant resistance, thereby having wide application prospects in agricultural production.

The present application relates to the technical field of environment-friendly and healthy pest prevention, and particularly relates to an environment-friendly and healthy pest prevention method for indoor planting, which comprises the following steps: establishing an interference barrier, increasing plant resistance, creating a natural enemy ecosystem and managing a circulation; a three-dimensional physical behavior interference barrier that cannot be found, entered or stayed by pests is established through the high-mesh gauze, natural repellent cotton yarn strips, differential microclimate disturbance and edible mineral particle spraying of step one. Meanwhile, the plant health and resistance are systematically enhanced through the local probiotic propagation, plant immune activator (willow branch liquid, mechanical stimulation) and functional companion plants of step two. The self-sustaining natural enemy ecosystem for pest control is established through the creation of natural enemy habitats (weed compost corners), preventive introduction and conservation of step three. The three levels work together to form an efficient, safe and chemical residue-free pest control system, which fundamentally guarantees the ecological environment and the quality and safety of agricultural products.