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Plant-immunity resistance inducer and application thereof

An immune inducer and plant technology, applied in the field of plant immune inducers, can solve the problems of increasing the cost of citrus production and sales, enhancing the drug resistance of Penicillium bacteria, and excessive pesticide residues in citrus.

Active Publication Date: 2019-07-30
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease is highly contagious and occurs frequently all over the world. Every year, about 20% of citrus are infected with penicillium, which brings huge losses to fruit production bases and sales enterprises.
[0003] In order to suppress the huge destructive power of citrus penicillium, people use chemical pesticides with the principle of bactericidal action, such as carbendazim, thiophanate-methyl, thiabendazole, benzoyl and other benzimidazole chemicals and promethazine amines, imazoles and other imidazoles, the long-term use of these pesticides in large quantities will affect human health and endanger the environment, and also enhance the drug resistance of penicillium
More importantly, these pesticides cannot be used in large quantities as preservatives for post-harvest citrus, because it is difficult to wash and remove them by effective means after spraying on citrus, resulting in too much pesticide residue in citrus
Moreover, the country has recently imposed strict restrictions on the use and residue of chemical preservatives (National Food Safety Standard Maximum Residue Limits of Pesticides in Food, GB 2763-2016), which has reduced the use of such bactericidal preservatives, but at the same time Intensified outbreak of postharvest citrus penicillium
However, if you use commonly used physical methods, such as low-temperature refrigeration and heat treatment methods, you need to consume a lot of energy and equipment, and transportation is inconvenient. Modified atmosphere preservation and packaging preservation require a special consumption of CO2. 2 and packaging materials, greatly increasing the production and marketing costs of citrus

Method used

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  • Plant-immunity resistance inducer and application thereof
  • Plant-immunity resistance inducer and application thereof
  • Plant-immunity resistance inducer and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The screening of embodiment 1 plant immune elicitor

[0032] The pathogen of Penicillium in postharvest citrus was isolated and identified by PCR amplification of 18S rDNA sequence, and the isolated pathogen was confirmed to be Penicillium fungus. The results were as follows: figure 1 As shown, the citrus tissue pieces severely infected with penicillium were isolated and cultured on potato medium. After several passages and purification, a single pathogen was isolated on the culture plate. After amplifying pathogenic 18SrDNA by PCR, about 1500 base fragments were obtained, and these base sequences were obtained by sequencing. After comparison with the NCBI database, it was confirmed that the isolated pathogen was a fungus of the genus Penicillium.

[0033] Screening for plant immune elicitors, including benzothiadiazole, activin, chitosan, sulfachloropyridazine, 2-amino-3,5-dichlorobenzoic acid, m-chlorobenzoic acid, salicylic acid, salicylic acid Acid methyl ester, F...

Embodiment 2

[0037] Determination of the content of each component in the plant immune inducer of embodiment 2

[0038] At normal temperature, 20mg / L, 30mg / L, 40mg / L of 2-amino-3,5-dichlorobenzoic acid and 30mg / L of 2-amino-3,5-dichlorobenzoic acid were applied with filler Immunization was carried out on post-harvest citrus, and the isolated citrus penicillium was inoculated on the immunized citrus, and the control was citrus without plant immune elicitor and no pathogen infection.

[0039] At room temperature, 0mg / L, 20mg / L, 30mg / L, 40mg / L of sulfachloropyridazine and 30mg / L of sulfachloropyridazine plus fillers were applied to post-harvest citrus for immunization, and then separated The citrus penicillium was inoculated on the immunized citrus, and the control was the citrus infected by the pathogen without the application of plant immune elicitors.

[0040] At room temperature, 0 mg / L, 1 g / L, 2 g / L, 3 g / L chitosan and 2 g / L chitosan plus fillers were applied to post-harvest citrus for ...

Embodiment 3

[0045] Embodiment 3 Plant immune inducer compares with traditional citrus disease-resistant and fresh-keeping means

[0046] Take 2-amino-3,5-dichlorobenzoic acid 0.3g, sulfachloropyridazine 0.2g, chitosan 30g, azadirachtin 0.5g, Pingpingjiao 9g and tea seed powder 10g to form the plant immunity The inducer is made into columnar particles with a diameter of 3 mm and a height of 5 mm through a granulator, and packed into a bag of granular immune inducer by a packaging machine for future use.

[0047] Traditional citrus disease-resistant and fresh-keeping methods use low-temperature storage at 13°C as a comparison of traditional physical disease-resistant and fresh-keeping methods, and in a room temperature environment, fruit wax, 0.5g / L imazalil and 0.5g / L thiophanate-methyl are selected as treatments. Compared with traditional chemical disease-resistant fresh-keeping methods; observe the effect of these disease-resistant fresh-keeping methods in inhibiting the development of c...

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Abstract

The invention provides a plant-immunity resistance inducer and application thereof. The active ingredients of the plant-immunity resistance inducer contain 2-amino-3,5-dichlorobenzoic acid, prinzone and chitosan. In this way, after the plant-immunity resistance inducer is used in harvested citrus, occurrence of harvested citrus immune penicilliosis can be induced, the damage effect of the penicilliosis on the quality of the harvested citrus can be effectively inhibited, and the preservative and fresh-keeping time of the harvested citrus can be prolonged. The plant-immunity resistance inducer is free of toxicity and pollution, capable of directly acting on the harvested citrus, and no pesticide residues are generated; the plant-immunity resistance inducer can effectively prevent anthracnoseof harvested mangoes.

Description

technical field [0001] The invention relates to the technical field of biological pesticides, in particular to a plant immune inducer and its application. Background technique [0002] Citrus penicillium is the most harmful and frequently occurring disease of postharvest citrus during storage and transportation. Its pathogen is Penicillium italicum Wehmer; Fungiaceae, Penicillium. The disease invades citrus fruits through wounds, or is infected through contact with sick and healthy fruits, resulting in a large number of rotten fruits. The disease is highly contagious and occurs frequently all over the world. Every year, about 20% of citrus are infected with penicillium and bring huge losses to fruit production bases and sales enterprises. [0003] In order to suppress the huge destructive power of citrus penicillium, people use chemical pesticides with the principle of bactericidal action, such as carbendazim, thiophanate-methyl, thiabendazole, benzoyl and other benzimidaz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154
CPCA23B7/154A23V2002/00A23V2200/10A23V2250/21A23V2250/511Y02A50/30
Inventor 汪文华何恩铭郭莺林志楷洪丽萍田淼洪晓光
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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