Method for improving fruit disease control effectiveness of antagonisitic yeast

A biocontrol yeast and disease control technology, which is applied in the field of improving the effectiveness of biocontrol yeast on fruit disease control, can solve the problems of limiting the activity of fruit biocontrol yeast, unclear genetic background information, and insufficient biocontrol physiological metabolism, etc., to achieve The effect of disease control is outstanding, the effect of increasing the antagonistic activity and improving the control effect

Inactive Publication Date: 2015-06-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, it has been reported at home and abroad that there is still a gap in the control effect of antagonistic yeasts that have been commercially developed compared with chemical fungicides.
Moreover, since the fruit disease biocontrol yeasts studied at home and abroad are all non-model yeasts, most of their genetic background information is not clear, and there are few studies on their biocontrol physiological metabolism, which severely limits the development of technology to improve the activity of fruit biocontrol yeasts.

Method used

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  • Method for improving fruit disease control effectiveness of antagonisitic yeast
  • Method for improving fruit disease control effectiveness of antagonisitic yeast
  • Method for improving fruit disease control effectiveness of antagonisitic yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, improve the method for biocontrol yeast to fruit disease control effect, carry out following steps successively:

[0051] First, the configuration of the culture medium:

[0052]Activation medium: Dilute 8g of beef extract, 5g of yeast powder, 10g of glucose, 20g of agar and 5g of β-glucan into 1000ml of water, and sterilize (121°C, 1.1 atmosphere, 30 minutes) to obtain activation culture base;

[0053] Seed medium: Dilute 8g of beef extract, 5g of yeast powder, 10g of glucose and 5g of β-glucan to 1000ml with water, and sterilize (121°C, 1.1 atmosphere, 30 minutes) to obtain a seed medium.

[0054] 2. Activation and preparation of strains:

[0055] 1), activation:

[0056] ①. Select Cryptococcus laurentis CGMCC NO.3590, which is stored in the activation medium at low temperature (4) ℃.

[0057] The above-mentioned Cryptococcus laurentii CGMCC NO.3590 was cultured in the activation medium at 28°C for 48 hours, and then subcultured twice under the same ...

Embodiment 2

[0066] Embodiment 2, only the activation medium and the seed medium are changed as follows, and all the other are the same as in Example 1;

[0067] Change the β-glucan in the activation medium from 5g to 10g, and the rest are the same as the activation medium in Example 1;

[0068] The β-glucan in the seed medium was changed from 5 g to 10 g, and the rest were the same as the seed medium in Example 1.

Embodiment 3

[0069] Embodiment 3, only the activation medium and the seed medium are changed as follows, and all the other are the same as in Example 1;

[0070] Change the β-glucan in the activation medium from 5 g to 1 g, and the rest are the same as the activation medium in Example 1;

[0071] The β-glucan in the seed medium was changed from 5 g to 1 g, and the rest were the same as the seed medium in Example 1.

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Abstract

The invention relates to the technical field of fruit postharvest disease control, and discloses a method for improving the fruit disease control effectiveness of antagonisitic yeast. The method comprises the following steps: activating antagonistic yeast, and carrying out liquid culture of the activated antagonistic yeast, wherein an activation medium used in the activation is obtained through adding water to 8g of a beef extract, 5g of yeast powder, 10g of glucose, 20g of agar, 1-10g of beta-glucan to 1000ml, and disinfecting; and each of a seed medium used in the activation and a seed medium used in the liquid culture is obtained through adding water to 8g of the beef extract, 5g of the yeast powder, 10g of glucose, and 1-10g of beta-glucan to 1000ml, and disinfecting. The above mediums can be used for the induction improvement of the fruit disease control effectiveness of the antagonisitic yeast (Cryptococcus laurentii having a preservation number of CGMCC NO.3590).

Description

technical field [0001] The invention relates to the technical field of post-harvest disease prevention and control of fruits, in particular to a method for improving the effectiveness of biocontrol yeast on fruit disease prevention and control. Background technique [0002] Traditionally, the control of fruit postharvest diseases at home and abroad mainly relies on fungicides. However, with the continuous enhancement of people's awareness of food safety and environmental protection, the scope and dosage of chemical fungicides are increasingly restricted. In developed countries in Europe and the United States, many chemical fungicides that are highly effective but have hidden dangers of food safety and environmental pollution are banned from use. Therefore, the research and development of methods and technologies that can replace chemical fungicides have great social significance and broad application prospects. [0003] Among the many researched methods that may replace ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38A23B7/155C12R1/645
Inventor 余挺曾丽珍郑晓冬余辰卢黄娉朱瑞瑜杨林
Owner ZHEJIANG UNIV
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