Botanical fungicide for preventing and controlling tobacco mosaic virus and preparation method

A plant-derived fungicide, tobacco mosaic virus technology, applied in the field of plant-derived pesticides, can solve problems such as poor effect, easy to produce drug resistance, environmental pollution, etc., and achieve good inhibitory effect

Inactive Publication Date: 2015-09-09
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some commonly used chemical agents are not effective, and ...

Method used

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  • Botanical fungicide for preventing and controlling tobacco mosaic virus and preparation method
  • Botanical fungicide for preventing and controlling tobacco mosaic virus and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] First, the whole plant of Asarum, the bud of cloves, the whole plant of Hellebore, and the different parts of four kinds of plants were dried and crushed through a 20-mesh sieve respectively, and then the raw materials prepared above were weighed by mass parts of Asarum 1, Clove 3, Veratrum 5, Ruixiang Langdu 6, mixed evenly, poured into the extraction tank, added methanol with 4 times the mass of the raw material, heated to 40 ° C while stirring, refluxed for 4 hours to obtain an extract, and then concentrated under reduced pressure. To obtain the paste, finally mix Tween 80 with 1 times the mass of the paste and mix it with the paste, then add 20% ethanol solution with 100 times the mass of the paste, to prepare a 20% ethanol solution for the prevention and treatment of tobacco mosaic virus disease. Botanical fungicide.

Embodiment 2

[0064] First, the whole plant of Asarum, the bud of cloves, the whole plant of Hellebore, and the different parts of four kinds of plants of Ruixiang Langdu root were respectively dried and pulverized to pass through a 40-mesh sieve, and then the raw materials prepared above were weighed according to mass parts of Asarum 3, Clove 12, Veratrum 8, Ruixiang Langdu 15, mixed evenly, poured into the extraction tank, added 7 times the mass of raw material ethanol, heated to 60 ° C while stirring, refluxed for 6 hours to obtain the extract, and then concentrated under reduced pressure. To obtain the paste, finally mix the Tween 80 with 3 times the mass of the paste and mix it evenly with the paste, then add 300 times the water of the paste to prepare the plant source sterilization for preventing and treating tobacco mosaic virus disease. agent.

Embodiment 3

[0066] First, the whole plant of Asarum, the bud of cloves, the whole plant of Hellebore, and the different parts of four kinds of plants of Ruixiang Langdu root were respectively dried, pulverized and passed through a 30-mesh sieve, and then the raw materials prepared above were weighed according to mass parts of Asarum 2, Clove 6, Veratrum 7, Ruixiang Langdu 10, mixed evenly, poured into an extraction tank, added 6 times the mass of raw material acetone, heated to 50°C while stirring, leached at constant temperature for 5 hours to obtain an extract, and then concentrated under reduced pressure After obtaining the paste, finally mix the Tween 80 with twice the mass of the paste and the paste, and then add 200 times the water of the paste to prepare a plant source for preventing and treating tobacco mosaic virus disease. fungicide.

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Abstract

The invention discloses a botanical fungicide for preventing and controlling tobacco mosaic virus. The botanical fungicide is prepared from the whole-plant mixed extracts of four plants, namely, herba asari, syzygium aromaticum, veratrum nigrum and stellera chamaejasme, as well as an additive and a solvent. Tests show that the botanical fungicide has a good inhibiting effect for the tobacco mosaic virus; either root-irrigation or spraying is adopted, indoor box plant tests show that the protection function is all 43% or above, and the inhibition rate in the therapeutical effect is all 73% or above; field tests show that the control efficiency is 68% or above, and is 20 percentage points higher than that of a chemical disinfectant. The raw materials adopted by the botanical fungicide are all natural plants which can be degraded in nature, no residue is left, and environmental harmony is achieved; besides antimicrobial active ingredients, the plant extracts also include many other ingredients which have the advantages and characteristics of being capable of promoting the plant growth, improving the plant immunity, increasing the disease resistance and the like, and have great significance in reducing the use of chemical pesticides, realizing environmental protection, and producing green and safe agricultural products.

Description

technical field [0001] The invention relates to a botanical pesticide, in particular to a botanical fungicide prepared by mixing active components extracted from four plants: asarum, clove, veratrum and daphne chamaejasme, and a preparation method of the fungicide. Background technique [0002] Viral diseases caused by plant viruses are important diseases in agricultural production. Tobacco mosaic virus (TMV) causes economic losses of more than 100 million US dollars every year. Since viruses are absolutely parasitic in plant cells, the energy, materials, and places required for their replication are completely provided by the host cells. In addition, plants do not have a complete immune and metabolic system, which makes the prevention and treatment of plant virus diseases more difficult. Antiviral agents can only delay or alleviate the symptoms of viral diseases to a certain extent, but cannot eradicate viruses, and have little effect on the treatment of plant viral disease...

Claims

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Application Information

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IPC IPC(8): A01N65/42A01P1/00
Inventor 曹挥莫海波李川刘湘琼赵慧琪郝晓娟赵慧琳
Owner SHANXI AGRI UNIV
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