36 results about "Paecilomyces spectabilis" patented technology

The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.

The invention relates to bacillus subtilis Pc3 and a use of bacillus subtilis Pc3 in preparation of a fermentation supernatant for preventing and controlling plant pathogenic fungi, and relates to bacilli. The bacillus subtilis Pc3 has a preservation number of CCTCC NO: M2013470. The bacillus subtilis Pc3 can prevent and control plant pathogenic fungi such as gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani and fusaria. A rape sclerotinia sclerotiorum prevention and control pot experiment proves that the bacillus subtilis Pc3 has good biological-prevention and control effects and has prevention and control efficiency of 95.6%. The fermentation supernatant of the bacillus subtilis Pc3 can be prepared by simple processes and has a wide fungus inhibition spectrum and good biological prevention effects.

The invention discloses an epipodium polycyclic aromatic hydrocarbon degrading bacterium and applications thereof. The epipodium polycyclic aromatic hydrocarbons degrading bacterium (paecilomyces variotii) F6 has the function of degrading a single pipodium polycyclic aromatic hydrocarbon and pipodium polycyclic aromatic hydrocarbon mixture. After the degrading bacterium is cultured for 30 days ina polycyclic aromatic hydrocarbon compound system, the degrading rate to the polycyclic aromatic hydrocarbon becomes 16.06 percent to 24.57 percent; however, the degrading rate in a single polycyclicaromatic hydrocarbons compound system becomes 10.42 percent to 33.25 percent, wherein, the degrading rates of benzo [k] fluoranthene and benzo [b] fluoranthene are the highest, which respectively account for 33.25 percent and 26.44 percent. The strain provided by the invention can provide new germplasm resources for developing the co-metabolism mechanism study of a plurality of epipodium polycyclic aromatic hydrocarbons as well as the construction of aromatic hydrocarbons combined pollution water-soil environment repairing technology.

The invention discloses a strain of Agaricus polyphagoides ( Pholiota Adiposa ) YX1, cultivation method and its application belong to the technical field of edible fungus. The lipopolyphaga YX1 has been deposited in the General Microorganism Center of China Microbiological Culture Collection Management Committee on December 1, 2020, and the strain preservation number is CGMCC NO.21077. The present invention adopts the Paecilomyces variotii fermentation liquid fermented liquid that endophytic fungus isolates to obtain as one of the raw materials of liquid culture medium, not only has increased Agaricum multiliposa ( Pholiota adiposa ) YX1 mycelium biomass, and reduce the cost of raw materials, to provide technical support for large-scale production. At the same time, the fermentation liquid of endophytic fungi is also fully utilized, which can promote the growth of other plants, without introducing other reagents, which is beneficial to the protection of the ecological environment.

The invention relates to the technical field of biological strains, particularly a Paecilomyces variotii strain and application thereof. The Paecilomyces variotii strain MM2 is collected to the China General Microbiological Culture Collection Center on July 8th, 2013, and the collection number is CGMCC No.7903. The application method comprises the following steps: adding the strain into any of straw-bran liquid culture medium, filter paper-inorganic salt liquid culture medium or PDA (potato dextrose agar) liquid culture medium, and culturing at 28-30 DEG C for 2-9 days to obtain the single-strain solid microbial inoculant. The Paecilomyces variotii strain can efficiently degrade and utilize cellulose, can be used as an external strain source for recycling lignocellulose solid waste, and can accelerate the degradation of the cellulose; the Paecilomyces variotii strain is separated from wine sediment compost and applied to wine sediment compost, thereby avoiding the problem of difficulty in assessment; and after being added, the microbial inoculant containing the strain effectively promotes the rotting of the wine sediment compost and accelerates the processing of the organic fertilizer.

The invention discloses a Liupao tea automatic pile fermentation strain composition which comprises a base bacterium solution, a consolidation bacterium solution and an aging bacterium solution. The bacterium solutions contain Aspergillus awamori, Aspergillus niger, Aspergillus tubingensis, penicillium citrinum, Aspergillus fumigatus, Eurotium rubrum, Aspergillus versicolor, Eurotium amstelodami, Paecilomyces variotii, Eurotium niveoglaucum, saccharomycetes and other beneficial microorganisms. On such basis, the inventor establishes the corresponding fermentation technique and equipment; and on the premise of not changing the upstream and downstream main production techniques of the original pile fermentation, the beneficial microorganism florae are added to control the growth of harmful infectious microbes, thereby enhancing the fermentation efficiency, shortening the pile fermentation period, and keeping the special thick flavor of the traditional Liupao tea. The pile fermentation automatic control system is utilized to dynamically monitor and control the changes on temperature, humidity and florae in the whole fermentation process, thereby implementing automation, continuity and high efficiency of the production technique, and ensuring the Liupao tea product to have safe and reliable quality and high and stable quality.

The invention discloses broad-spectrum fungus-resistant bacillus and application thereof to preventing wheat scab and relates to bacillus. The preservation number of the bacillus (Bacillus sp.)DY26-010 is CCTCC NO:M2015407. Supernatant liquid of the bacillus is antagonistic to fusarium spp, gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia, mutualism alternaria, botrytis cinerea and colletotrichum dematium. Fermentative supernatant liquid of the bacillus DY26-010 has high antagonism on pathogenic bacteria and fusarium spp of wheat scab, the preventing effect can be 57.1% in a potted experiment of wheat scab, and the bacillus can be used for preparing the fermentative supernatant liquid applied to preventing wheat scab. A biological prevention method of wheat scab is provided, no chemical pesticide is used, the bacillus has the advantages of being non-toxic, not prone to forming a pesticide-resistant environment, biologically safe and the like, and application prospects are good.

The invention discloses a Paecilomyces variotii strain with strong pathogenicity to citrus psyllids and an application thereof, belonging to the technical field of biological control. The strain is Paecilomyces variotii GZMS‑11, which was deposited in China Center for Type Culture Collection on October 29, 2015, with the preservation number CCTCC NO: M 2015651. Paecilomyces variotii GZMS-11 described in the present invention has strong pathogenicity to citrus psyllids, and can be developed as a biological pesticide for the biological control of citrus psyllids, compared with the current chemical agents for preventing and treating citrus psyllids , has the advantages of high efficiency, green, environmental protection, long-lasting effect and not easy to produce drug resistance, and has no pollution to the environment and no residue. The strain GZMS-11 has fast growth speed, large spore production, high spore germination rate, is easy to prepare, and has good market application prospects.

The invention discloses paecilomyces variotii powder as well as a preparation method and an application thereof. The powder is prepared by fermenting paecilomyces variotii with a preservation number of CGMCC No.13772 on a corn flour culture medium. The paecilomyces variotii powder has the beneficial effects that (1) paecilomyces variotii can parasitize nematodes and simultaneously produce multiple secondary metabolites so as to inhibit the growth of the nematodes, so that the paecilomyces variotii powder can be used for effectively preventing and treating the nematodes; (2) the paecilomyces variotii powder is high in spore content and nematode killing efficiency and long in guarantee period; and (3) a preparation process of the paecilomyces variotii powder is simple and suitable for industrial production.

The invention relates to bacilli, in particular to a bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi. A preservation number of the Bacillus sp.DY26-004 is CCTCC NO:M 2015406. The bacillus DY26-004 has a strong inhibiting effect on various plant pathogenic fungi such as fusaria, colletotrichum musae, paecilomyces variotii, sclerotinia scleotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani, alternaria alternata, botrytis cinerea and colletotrichum gloeosporioides, and has good biological prevention and control effects in pot experiments for prevention and control of sclerotinia scleotiorum and fusarium graminearum, wherein control efficiencies are 72% and 65% respectively. The bacillus DY26-004 which is a nontoxic, non-pathogenic and environment-friendly bacillus is a useful resource for research and development of microbial pesticides and has a potential application prospect.

The invention discloses broad-spectrum plant-pathogenic-fungus-resistant bacillus and application thereof and belongs to bacillus. The preservation number of the bacillus (Bacillus sp.) DY26-014 is CCTCC NO:M2015408. The bacillus (Bacillus sp.)DY26-014 can be used for preparing plant pathogenic fungus fermentative supernatant liquid. Plant pathogenic fungi prevented by the bacillus DY26-014 comprise fusarium spp, gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia, mutualism alternaria, botrytis cinerea and colletotrichum dematium. The broad-spectrum plant-pathogenic-fungus-resistant bacillus has a good effect in a potted experiment for preventing sclerotinia rot of colza, and the preventing effect can be 61.9%. Fermentative supernatant liquid of the bacillus (Bacillus sp.)DY26-014 has the advantages that the preparation technology is simple, the fungus suppression spectrum is broad, and the action effect is remarkable.