The invention discloses a method for preparing an extract rich in
ginsenoside Rd by fermenting pseudo-
ginseng with
paecilomyces farinosus, and belongs to the technical field of microbial
fermentation. The method specifically comprises the following steps: crushing and sieving pseudo-
ginseng main roots, pseudo-
ginseng incisions and pseudo-ginseng stems and leaves, collecting
powder with different granularities, and mixing the
powder with different granularities to obtain pseudo-ginseng main root coarse
powder, pseudo-ginseng incisions coarse powder and pseudo-ginseng stem and leaf coarse powder; preparing pseudo-ginseng powder; preparing a pseudo-ginseng culture medium; the preparation method comprises the following steps: co-culturing
paecilomyces farinosus and a pseudo-ginseng culture medium to obtain a fermented
suspension culture; the preparation method comprises the following steps: dissolving
panax notoginseng into a
suspension culture, evaporating the
suspension culture to remove water to obtain
panax notoginseng plastid, performing
reflux extraction on the
panax notoginseng plastid, concentrating an extracting solution to obtain extract, dissolving the extract with water, adsorbing an
aqueous solution of the extract into resin, eluting, concentrating an eluent, and
drying to obtain the extract rich in
ginsenoside Rd. The method disclosed by the invention has the advantages of simplicity in operation, no environmental
pollution and high efficiency.