Method for preventing and controlling greenhouse soil continuous cropping obstacles by using endophytic fungus secondary metabolites

A technology of secondary metabolites and soil continuous cropping obstacles, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc., can solve threats to the quality and safety of horticultural products in facilities that cannot be fundamentally eliminated Continuous cropping obstacles, poor soil micro-ecological environment and other problems, to achieve the effect of improving crop system resistance, significant control effect, and reducing pesticide residues

Active Publication Date: 2022-04-26
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the impact of economic benefits and implementation costs, intercropping and crop rotation are not widely used in production; grafting is an effective measure to prevent continuous cropping obstacles in production, but it cannot fundamentally eliminate continuous cropping obstacles, but only delays the occurrence of soil continuous cropping obstacles. Because the rootstock itself will also undergo continuous cropping
In addition, for

Method used

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  • Method for preventing and controlling greenhouse soil continuous cropping obstacles by using endophytic fungus secondary metabolites
  • Method for preventing and controlling greenhouse soil continuous cropping obstacles by using endophytic fungus secondary metabolites
  • Method for preventing and controlling greenhouse soil continuous cropping obstacles by using endophytic fungus secondary metabolites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation of endophytic fungal secondary metabolites

[0043] Inoculate the Paecilomyces variotii strain SJ1 with the preservation number CGMCC NO.10114 on the PDA medium, culture it at 25°C for 6 days, and inoculate it into the Erlenmeyer flask containing the seed liquid medium with a puncher agar cultured on a rotary shaker at 28°C and 120r / min for 3 days as a seed solution. The seed solution was inserted into a Erlenmeyer flask containing a fermentation medium at an inoculation amount of 10% (volume percentage), and cultured on a rotary shaker at 28° C. and 120 r / min for 5 days to terminate the fermentation.

[0044] The cultured mycelium was washed, dried at 60°C, weighed, crushed, extracted with the same amount of absolute ethanol for 24 hours, extracted 3 times, combined with the extracts, mixed with a magnetic stirrer, and ultrasonically oscillated After 1 hour, vacuum filter and collect the filtrate to obtain the secondary metabolites of endophytic...

Embodiment 2

[0045] Example 2: Investigation of the effect of endophytic fungal secondary metabolites on the prevention and control of facility soil continuous cropping obstacles

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Abstract

The invention discloses a method for preventing and controlling facility soil continuous cropping obstacles by using endophytic fungus secondary metabolites, and belongs to the technical field of facility horticulture. The secondary metabolite of the endophytic fungus Paecilomyces variotii strain SJ1 can effectively improve the crop system resistance, improve the resistance to continuous cropping soil stress such as soil acidification, secondary salinization, root-knot nematode and the like, promote root development, enhance the resistance of crops to the continuous cropping soil stress, relieve the harm of greenhouse soil continuous cropping obstacles, and improve the crop yield. And the method becomes an important technical means for green ecological prevention and control of greenhouse soil continuous cropping obstacles.

Description

technical field [0001] The invention relates to the technical field of facility gardening, in particular to a method for preventing and controlling facility soil continuous cropping obstacles by utilizing secondary metabolites of endophytic fungi. Background technique [0002] my country is a big producer of facility agriculture, and the area of ​​greenhouses accounts for more than 85% of the total area of ​​facility agriculture in the world. Obstacles to continuous cropping are ubiquitous in facility crop production, and have become the biggest limiting factor restricting the development of facility agriculture in my country. Continuous cropping obstacle refers to the phenomenon of reduced yield, poor quality and poor growth status after continuous cropping of the same crop or related crops, even under normal cultivation and management conditions. The causes of continuous cropping obstacles are complex. Generally, continuous cropping obstacles are attributed to the deterio...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01P5/00A01P21/00A01B79/02C12N1/14C12R1/79
CPCA01N63/30C12N1/14A01B79/02
Inventor 张大龙魏珉李岩陈晓璐李晓甜娄洁李蓬琪
Owner SHANDONG AGRICULTURAL UNIVERSITY
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