Method for preventing and controlling greenhouse soil continuous cropping obstacles by using endophytic fungus secondary metabolites
A technology of secondary metabolites and soil continuous cropping obstacles, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc., can solve threats to the quality and safety of horticultural products in facilities that cannot be fundamentally eliminated Continuous cropping obstacles, poor soil micro-ecological environment and other problems, to achieve the effect of improving crop system resistance, significant control effect, and reducing pesticide residues
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Embodiment 1
[0042] Example 1: Preparation of endophytic fungal secondary metabolites
[0043] Inoculate the Paecilomyces variotii strain SJ1 with the preservation number CGMCC NO.10114 on the PDA medium, culture it at 25°C for 6 days, and inoculate it into the Erlenmeyer flask containing the seed liquid medium with a puncher agar cultured on a rotary shaker at 28°C and 120r / min for 3 days as a seed solution. The seed solution was inserted into a Erlenmeyer flask containing a fermentation medium at an inoculation amount of 10% (volume percentage), and cultured on a rotary shaker at 28° C. and 120 r / min for 5 days to terminate the fermentation.
[0044] The cultured mycelium was washed, dried at 60°C, weighed, crushed, extracted with the same amount of absolute ethanol for 24 hours, extracted 3 times, combined with the extracts, mixed with a magnetic stirrer, and ultrasonically oscillated After 1 hour, vacuum filter and collect the filtrate to obtain the secondary metabolites of endophytic...
Embodiment 2
[0045] Example 2: Investigation of the effect of endophytic fungal secondary metabolites on the prevention and control of facility soil continuous cropping obstacles
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