78 results about "Rhizoctonia solani" patented technology

The invention belongs to the technical field of microorganisms and biological control, and particularly relates to paenibacillus polymyxa and application thereof in biocontrol and growth promotion. Specifically, a strain of paenibacillus polymyxa GLZ219 is obtained through screening, and experiments prove that the paenibacillus polymyxa GLZ219 has a relatively high bacteriostasis rate on pathogenic bacteria of tomato neck and root rot; meanwhile, the strain has a good antagonistic effect on nine common plant pathogens such as Rhizoctonia solani and the like, and can be widely applied to green prevention and control of various crop diseases. Besides, the paenibacillus polymyxa GLZ219 has nitrogen fixing and potassium dissolving capabilities, can secrete heteroauxin and siderophores, and can effectively promote the growth of tomato plants, the plant height of tomato seedlings treated by fermentation liquor of the paenibacillus polymyxa GLZ219 is remarkably increased, diseases can be prevented and controlled, the growth vigor of crops can be improved, the yield and quality of the crops can be improved, and the paenibacillus polymyxa GLZ219 has a wide application prospect.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The invention discloses bacillus velezensis as well as a biological preparation and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis is rice endophytic bacillus velezensis, is classified and named as bacillus velezensis HA-B13, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M 20251098, shows extremely excellent biological activity to nigatosporium oryzae, has the antibacterial rate of 94%, and can be used for preparing the bacillus velezensis. Meanwhile, the bacillus subtilis also has relatively good biological activity on a plurality of rice pathogenic bacteria such as rhizoctonia solani, pyricularia grisea, gylastoma gonoryzae and the like. And the strain is derived from rice plants and can be well colonized in the rice plants, so that the strain has a long-acting prevention and treatment effect on diseases such as spike rot and the like, can effectively reduce the investment of rice disease prevention and treatment products, is safer and has great prevention and treatment application potential.

The invention relates to the technical field of microorganisms, and particularly discloses bacillus deserticola for resisting tobacco target leaf spot, a bacterial drug compound and application of the bacillus deserticola and the bacterial drug compound. The bacillus deserticola is bacillus deserticola 127-2 and is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC No.34058, and the bacillus deserticola is classified and named as Bacillus inaquorum; the in-vessel inhibition rate of the strain 127-2 to tobacco target leaf spot caused by rhizoctonia solani is 87.5%, the in-vivo inhibition activity also reaches 50% or above, the in-vivo inhibition activity of the strain 127-2 to tobacco target leaf spot caused by compounding of the strain 127-2 and pyraclostrobin reaches 98% or above, and the purposes of drug reduction, persistent effect and efficient prevention and control of rhizoctonia solani can be effectively achieved through the synergistic effect of bacteria and drugs.

The invention discloses a streptomyces albidoflavus strain SaTC2601 and application thereof, and belongs to the field of biological control. According to the invention, a streptomyces albidoflavus strain SaTC2601 is separated from faeces of larvae of WSFC, and the preservation number of the streptomyces albidoflavus strain SaTC2601 is CGMCC (China General Microbiological Culture Collection Center) No.33741. The strain SaTC2601 has a very good prevention and treatment effect on peanut southern blight caused by sclerotium rolfsii at peanut roots, also has a prevention and treatment effect on soil-borne pathogenic bacteria such as fusarium graminearum, fusarium solani, fusarium moniliforme, fusarium oxysporum, rhizoctonia solani and streptomyces scabies, and further, the strain SaTC2601 also has a very good growth promoting effect. The strain becomes a precious resource for new drug development and agricultural disease prevention and control, and is expected to play a huge role in the aspect of soil disease prevention and control.

The application discloses application of undecyl-prodigiosin in inhibition of a root rot pathogen (Fusarium oxysporum) of Salvia miltiorrhiza, and belongs to the technical field of microbial pesticides. 25 H 33 The compound has a molecular formula of C 50 N3O, is derived from Streptomyces aurantiacus YS-4, has a concentration-dependent inhibitory effect on the Fusarium oxysporum, an EC of 33.1 μg / mL, and an inhibition rate of 83.3% at 400 μg / mL. The application further provides a preparation containing the compound, wherein the concentration of the compound is not less than 20 μg / mL, and the concentration of the target area after application is controlled in a range of 20 μg / mL to 400 μg / mL, so that the root rot of Salvia miltiorrhiza can be effectively prevented and treated. As a natural microbial metabolite, the compound is environment-friendly, has no residue, and is not easy to produce drug resistance, solves the pollution problem of chemical prevention and treatment, and provides a new scheme for green prevention and treatment of the root rot of Salvia miltiorrhiza.

The invention belongs to the technical field of pesticide chemistry, and particularly relates to an application of a substituted phenylhydrazine copper salt complex as an agricultural fungicide, the structural general formula of the substituted phenylhydrazine copper salt complex is [Cu (ArNHNH2) 2] Xn, Ar is selected from 3-chlorphenyl-, 2, 4-dichlorophenyl-, 2, 4-dichlorophenyl-, 2, 4-dichlorophenyl-, 2, 4-dichlorophenyl-, 2, 4-dichlorophenyl-, 2, 4-dichlorophenyl-, 2, 4-dichlorophenyl-, 2, 4-dichlorophenyl-, 2, 4-dichlorophenyl-, the compound is prepared from 2, 6-dichlorophenyl, p-trichloromethoxyphenyl, 4-trifluoromethylphenyl, 4-trifluoromethoxyphenyl, 4-bromophenyl, 4-nitrophenyl, 4-cyanophenyl and 4-carboxyphenyl, and the compound is prepared from 2, 6-dichlorophenyl, p-trichloromethoxyphenyl, 4-trifluoromethylphenyl, 4-trifluoromethoxyphenyl, 4-bromophenyl, 4-nitrophenyl, 4-cyanophenyl and 4-carboxyphenyl. X is selected from SO4 <->, Cl <-> and NO3 <->; n is equal to 1-2. The substituted phenylhydrazine copper salt complex disclosed by the invention has remarkable inhibitory activity on plant pathogenic bacteria, has very high inhibition rate on three plant pathogenic fungi, namely fusarium graminearum, rhizoctonia solani and sclerotium rolfsii, can be used as a safe and efficient novel bactericide, and is applied to prevention and treatment of various crop diseases.

This invention belongs to the field of microbial technology, specifically relating to a strain of Acetobacter peroxide 672 and its applications. The Acetobacter peroxide 672 strain of this invention can enhance the antagonistic activity of Pichia pastoris against Rhizoctonia solani and also antagonizes Colloidal anthracnose, providing a multi-faceted solution for the biological control of crop diseases. Furthermore, the strain possesses the ability to produce indoleacetic acid, which can significantly promote the growth and development of grain crops such as wheat and corn, promote root expansion, enhance nutrient and water absorption, and thus effectively increase biomass. In addition, the strain can tolerate and degrade chemical pesticide components such as imidacloprid and carbendazim, alleviating soil pesticide residue pollution, and can effectively alleviate the inhibitory effects of low pH stress on crops, enhancing their stress resistance. Therefore, the Acetobacter peroxide 672 strain of this invention has significant application value in the biological control of crop diseases, crop growth promotion, soil pesticide residue remediation, and enhancing crop stress resistance.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The application provides a Rhodiola kirilowii root rot harmless prevention and control method and relates to the technical field of plant disease prevention and control. The Rhodiola kirilowii root rot harmless prevention and control method is characterized by the following steps: after seed disinfection, the seed is treated by immersion in a solution of a Yunnan sclerotia of Sclerotinia spore powder extract, and a fermentation substrate of Bacillus subtilis and Bacillus amyloliquefaciens is used, and then the roots are irrigated with a solution of a Yunnan sclerotia of Sclerotinia spore powder extract and a Trichoderma harzianum liquid. The method can effectively prevent and control the Rhodiola kirilowii root rot, reduce the use of chemical pesticides, reduce pesticide residues, and improve the safety of planting.

The application belongs to the field of agricultural fungicide compound, discloses a composition containing new white folium altheae alkaloid derivative Z-24 and anthraquinone components (emodin, emodin methyl ether, chrysophanol, aloe emodin) in rhubarb and mixed concentration thereof. The application of the composition in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, pyricularia oryzae and phytophthora capsici is disclosed, and the mass percentage of new white folium altheae alkaloid derivative Z-24 and anthraquinone components is 1:500, 1:100, 1:50 or 1:25. The fungicidal composition disclosed in the application has the advantages of wide fungicidal spectrum, low toxicity, effective delay of drug resistance of plant pathogens and the like, and has the value of further research and development.

The application belongs to the technical field of chemical synthesis, and particularly relates to a trifluoromethyl-containing benzothiazole tertiary alcohol derivative, a preparation method and application thereof. 1 , R 2 are independently selected from hydrogen, C1-C3 alkyl, C1-C3 alkoxy or halogen; or R 1 , R 2 independently form a naphthalene ring with the substituted benzene ring together. The trifluoromethyl-containing benzothiazole tertiary alcohol derivative provided by the application has good bacteriostatic effect on one or more of Rhizoctonia solani, Fusarium moniliforme, Fusarium graminearum, Fusarium oxysporum and Phytophthora nicotianae.

The invention discloses a quinolinic acid diaryl ether derivative, a preparation method and application thereof and a bacteriostatic agent or bactericide, relates to the technical field of medicinal chemistry, solves the problems that existing pesticides are high in toxicity and not environmentally friendly, easily cause drug resistance of pathogenic bacteria and possibly have negative effects on the environment and an ecological system, can efficiently prevent and treat fungal diseases of plants, and also can effectively prevent and treat the pathogenic bacteria of the plants. The quinolinic acid diarylether derivative has the advantages that the quinolinic acid diarylether derivative has excellent inhibition activity on agricultural fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum and fusarium pseudograminearum, and the quinolinic acid diarylether derivative has the characteristics of simple synthesis process, cheap and easily available raw materials, good control effect, wide bactericidal spectrum and the like.

The invention discloses a microbial bacterium inhibitor as well as a preparation method and application thereof, the microbial bacterium inhibitor is fermentation liquor obtained by activating and fermenting bacillus siamensis YWF-25-21, and the microbial bacterium inhibitor has a relatively good prevention and treatment effect on tobacco black shank, fusarium root rot and rhizoctonia solani. The bacillus siamensis microbial agent prepared by the invention is high in spore content, can reduce crop disease loss, can effectively promote crop plant growth and development, and can enhance crop stress resistance; the method can meet the requirement of batch industrial production, ensures that products are more stably supplied on the market, directly reduces the production cost, improves the economic benefits, and is suitable for popularization and application.

The invention belongs to the technical field of plant disease control, and particularly relates to application of amide compounds or agrochemically acceptable salts thereof in preparation of antifungal drugs. The amide compound or the acceptable salt of the amide compound shows remarkable broad-spectrum antibacterial activity on various plant pathogenic fungi such as rhizoctonia solani, tobacco target leaf spot bacteria and magnaporthe oryzae, and has no visible phytotoxicity symptom on plants in an effective concentration range. The action mechanism research shows that the amide compound or the acceptable salt thereof inhibits the growth of fungi through multiple ways: (1) the biological wall and cell membrane structures of the fungi are destroyed to cause the outflow of contents so as to play a bactericidal role; (2) the physiological function of fungal normal cells is interfered, so as to induce programmed death of fungal cells. The invention provides an important reference way for developing a high-efficiency novel agricultural bactericide.

The invention discloses a bacteriostatic slow-release water-saving type hole storage ball based on industrial hemp stalks and a preparation method thereof.The hole storage ball is prepared from, by mass, 5-10 parts of industrial hemp stalk powder, 30-50 parts of montmorillonite powder, 2-10 parts of activated carbon, 5-10 parts of a water-retaining agent, 5-20 parts of a water-soluble nitrogen-phosphorus-potassium compound fertilizer and 20-40 parts of water; the montmorillonite powder, the activated carbon and the water-soluble nitrogen-phosphorus-potassium compound fertilizer are premixed and then secondarily mixed with the industrial hemp stalk powder and the water-retaining agent, and granulation molding is performed to prepare the hole storage ball. According to the invention, antibacterial substances such as cannabinol naturally contained in the waste industrial hemp stalks are fully utilized, so that the fertilizer block is endowed with an internal plant health-care function, the hole storage ball effectively inhibits soil-borne pathogenic bacteria such as fusarium and rhizoctonia solani, the phenomena of root rot and seedling death of crops are reduced, the use of chemical pesticides is reduced, and the production cost is reduced. The problems of crop water retention, bacteriostasis and soil improvement can be solved simultaneously, waste is turned into wealth, and the method is suitable for popularization and application.

The invention belongs to the technical field of microbial control, and particularly relates to a soybean root rot biocontrol bacterium with growth-promoting and yield-increasing functions, a bacterium agent, a preparation method and application. The biocontrol bacterium is a salt-tolerant bacillus, is named as GY6, is preserved in the China Center for Type Culture Collection (CCTCC) on November 12, 2025, and has the preservation number of CCTCC M 20252537. The salt-tolerant bacillus GY6 has soybean root rot antagonistic ability, and can play a growth promoting effect through IAA production, phosphate solubilization, potassium release and the like. Through synergistic promotion of disease prevention and control and healthy growth of crops, the GY6 strain can achieve synergistic improvement of crop yield and quality, and an effective way is provided for green yield increase of soybeans. The microbial inoculum prepared from the GY6 has positive promotion effects on reducing agricultural non-point source pollution, promoting soil health and even constructing a sustainable farmland ecosystem.

The invention provides an RPA primer / probe combination for detecting rhizoctonia solani AG-3TB and application of the RPA primer / probe combination, and belongs to the technical field of phytopathogen detection. The RPA primer comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID NO. 2; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO. 5; the nucleotide sequence of the probe is as shown in SEQ ID NO. 7. According to the present invention, based on the AG-3TB subgroup strain whole gene series, the specific fluorescent primer and the probe are autonomously designed, the efficient and sensitive method suitable for rapid detection is developed, the specificity is strong, and the AG-3TB subgroup can be distinguished.

The invention relates to novel acridine spiroisoxazole compounds, a preparation method thereof and application of the novel acridine spiroisoxazole compounds in antifungal drugs. The novel acridine spiroisoxazole compounds have the antibacterial activity of 36 compounds, and the 36 compounds have the inhibiting effect on botrytis cinerea, parasite scalaria, rhizoctonia solani, fusarium graminearum and candida albicans. The antifungal biological activity result shows that the compound series all show obvious antibacterial activity. The compound is determined as a broad-spectrum antibacterial candidate due to the comprehensive antibacterial characteristics of the compound, and the EC50 value (5.0 + / -0.4 mu g / mL) of H35 shows 1.5 times, 2.2 times and 3.2 times of titer advantages compared with positive control drugs famoxadone (7.6 mu g / mL), chlorothalonil (11.1 mu g / mL) and carbendazim (15.9 mu g / mL) respectively. The spiro structure of the compound can significantly improve the antibacterial activity of the compound, the preliminary mechanism research target shows that the compound is related to fungal membrane damage, and in addition, the compound H35 has protection and treatment effects on botrytis cinerea infection in corn, which shows that the compound H35 has agricultural application potential.

The invention belongs to the technical field of microbial control, and particularly relates to a cigar tobacco root rot biocontrol bacterium with root strengthening and growth promoting functions, a bacterium agent, a preparation method and application. The biocontrol bacterium is bacillus subtilis, is named as SY2, is preserved in the China Center for Type Culture Collection on October 15, 2025, and has the preservation number of CCTCC M 20252220. The bacillus subtilis can be used for preparing the cigar tobacco root rot biocontrol microbial inoculum with root strengthening and growth promoting functions, the bacillus subtilis is colonized in a CX-14 rhizosphere for a long time by utilizing the specific affinity of the bacillus subtilis and cigar tobacco CX-14, the growth promoting and root rot preventing and treating effects can be stably exerted, and the lodging resistance of cigar tobacco is improved.

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.

The invention provides a preparation method and application of methyl 2-hydroxyphenylacetate. The methyl 2-hydroxyphenylacetate is separated from a fermentation culture of streptomyces wetland 13-3 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 22830. According to the method, the compound methyl 2-hydroxyphenylacetate is obtained from streptomycete for the first time, verification proves that the methyl 2-hydroxyphenylacetate has broad-spectrum antibacterial activity on various plant pathogenic fungi, particularly, the inhibitory activity on aspergillus fumigatus, colletotrichum truncatum, rhizoctonia solani, verruca verrucosa and magnaporthe oryzae is higher than that of amphotericin B, and the methyl 2-hydroxyphenylacetate can be used for preparing antibacterial agent drugs and has broad-spectrum antibacterial activity. A novel natural antibacterial compound is provided for the fields of agriculture and medicine, and the method is simple and convenient to operate, high in purification efficiency and environment-friendly.

The present disclosure belongs to the technical field of plant disease control, and specifically relates to use of 4-(diethylamino)salicylaldehyde in controlling a phytopathogen, a method for controlling a phytopathogen, and a bactericide. The 4-(diethylamino)salicylaldehyde shows an excellent inhibitory effect on the phytopathogen (including a phytopathogenic fungus and / or a phytopathogenic bacterium), especially has the best inhibitory effect on Rhizoctonia solani and Erwinia amylovory, and exhibits a potential to be developed into a novel bactericide.

The invention discloses preparation and application of a novel sclerotium-eating fungicide. The invention provides a combined bacterium, which is prepared from Intraporporus sclerotiporus S003 (CGMCC (China General Microbiological Culture Collection Center) No.35463) and Bacillus amyloliquefaciens L1017 (CGMCC No.36133). The invention also provides a preparation method of the combined bacterium, and the combined bacterium is prepared from the following raw materials: the Intraporporus sclerotiporus S003, the Bacillus amyloliquefaciens L1017, the Bacillus amyloliquefaciens L1017 and the Bacillus amyloliquefaciens L1017, the Bacillus amyloliquefaciens L1017 and the Bacillus amyloliquefaciens L1017. The complex microbial inoculant composed of the bacillus subtilis and the bacillus subtilis has a synergistic inhibition function on rhizoctonia solani CGMCC (China General Microbiological Culture Collection Center) 3.7376. The bacillus subtilis and the bacillus subtilis have a synergistic inhibition function on rhizoctonia solani. The two functional bacteria are scientifically screened and compounded to form a stable compound microbial agent, and the compound microbial agent is used for disease prevention and control and crop growth promotion and has wide application prospects and industrial value.

This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition containing benzimidazole. The pesticide composition's active ingredient is a compound of benzimidazole and fluazinam or emodin methyl ether. This invention, through indoor bioactivity tests, found that the compound of benzimidazole with fluazinam or emodin methyl ether, within a certain mass ratio range, has a synergistic effect against various plant fungal diseases such as Rhizoctonia solani, Curvularia crescentis, and Sclerotium sclerotiorum, helping to improve control efficacy. This is of great significance for the research and development of pesticides for controlling diseases such as cucumber damping-off, corn curvularia leaf spot, and peanut white mold.

The application belongs to the field of pesticides, and discloses a compound with the structure of formula I, a preparation method of the compound, and the compound is prepared by the following steps: reacting piperazine and piperazine dihydrochloride in hot ethanol, then sequentially reacting with different benzyl chlorides and sodium hydroxide solution to obtain corresponding monosubstituted piperazine; subsequently, methyl anthranilate is sequentially reacted with formamide, sulfoxide, coumarin molecules hydrolyzed by sodium hydroxide and monosubstituted piperazine to obtain the cinnamic acid amide derivative containing quinazoline and piperazine units; the cinnamic acid amide derivative containing quinazoline and piperazine units is a novel compound, has inhibitory effect on botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum or rhizoctonia solani, and can be used for preventing and treating plant diseases caused by fungi.

The present invention discloses a benzopyran derivative, a synthesis method, and an application thereof. Trifluoroacetone containing a benzene ring and different heterocycles and a substituted salicylaldehyde are used as raw materials, DMSO is used as a solvent, and a reflux reaction is performed to generate a crude benzopyran derivative with different substituents. The crude product is post-treated to obtain the benzopyran derivative. The reaction is tracked by TLC or liquid chromatography, and the reaction process is simple and easy to operate. Eighteen synthesized compounds were subjected to antifungal activity experiments to determine the antifungal activity of the compounds against Fusarium graminearum, Rhizoctonia solani, Fusarium moniliforme, and Fusarium oxysporum. At 200 ppm, thiophene dibromopyrone had the highest inhibition rate against Fusarium graminearum, at 98.84%; unsubstituted dichloropyrone had the highest inhibition rate against Fusarium graminearum, at 66.08%; pyridine dichloropyrone had the highest inhibition rate against Fusarium oxysporum, at 71.07%; and pyridine dichloropyrone had the highest inhibition rate against Fusarium moniliforme, at 69.12%. The benzopyran derivatives synthesized by the present invention will show good application prospects in various fields such as medicine, organic synthesis, biosensing and optoelectronic materials.

This invention provides a strain of *Pseudomonas aeruginosa* RL76, its bacterial suspension, its preparation method, and its applications, belonging to the field of agricultural microbiology technology. The *Pseudomonas aeruginosa* RL76 was deposited at the China General Microbiological Culture Collection Center (CGMCC) on January 26, 2026, with accession number CGMCC No. 37531. This invention utilizes a mixture of filter mud and soil for sugar beet cultivation. It was found that, under the same pathogen inoculation conditions, the filter mud treatment significantly alleviated the occurrence of damping-off disease in sugar beets compared to the non-filter mud treatment, suggesting that the filter mud plays a role in increasing the relative abundance of beneficial microorganisms in the soil. Therefore, this invention isolated a strain of *Pseudomonas aeruginosa* RL76 with highly efficient antagonistic activity against *Rhizoctonia solani* from the rhizosphere soil of sugar beets cultivated using a mixture of filter mud and soil, and for the first time discovered its effective control of sugar beet damping-off disease while also promoting sugar beet growth, representing a groundbreaking achievement.

The invention discloses a salt-tolerant nitrogen-fixing Klebsiella pasteurii and application thereof, and relates to the technical field of microorganisms, and the Klebsiella pasteurii S8812 provided by the invention is a salt-tolerant combined nitrogen-fixing bacterial strain which is screened from corn roots and has outstanding comprehensive performance; the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 21, 2025, and the preservation number of the strain is CGMCC No: 33923. The strain is high in nitrogen fixation activity, strong in salt resistance and capable of tolerating a culture medium environment with the NaCl concentration as high as 7%. Meanwhile, the strain also has the functions of dissolving organic phosphorus, dissolving potassium, producing amylase, producing ammonia, producing siderophore, producing auxin and the like. Greenhouse pot experiments show that the strain has a growth promoting effect on various crops such as wheat and corn, and the plant height, stem length, fresh weight and the like of plants can be remarkably improved. In addition, the strain has a good inhibition effect on rhizoctonia solani, pectobacterium and xanthomonas oryzae.

The present invention relates to the field of chemical synthesis, and more specifically to a 3-spiroindolone derivative, its preparation method, and application. This application prepares a 3-spiroindolone derivative and applies it to combat forest-derived fungi. It is found that compared with the prior art, the synthesized derivative has moderate inhibitory effects against Rhizoctonia solani, Solanum solani, and Aspergillus pinecone, providing a new direction for the prevention and control of forest pests and diseases.