400 results about "Rhizoctonia solani" patented technology

The invention discloses a trichoderma viride fungi strain and a method for preparing a bio-control fungicide thereof and application. The strain is named as trichoderma viride strain ZBS6 and collected in the CGMCC, and the collection number is CGMCC No.3538. The microbial fungicide is liquid or solid fungicide, and is prepared from the trichoderma viride ZBS6 strain. The ZBS6 strain and the fungicide thereof have wide bacteriostatic spectrum and good bacteriostatic effect, and have strong inhibiting effect on botrytis cirerea, rhizoctonia solani, rhizocto-nia cerealis, gaeumannomyces grami, marssonina coronaria, fusarium oxysporum and the like; and the fungicide can be used for control of botrytis cirerea, cucumber downy mildew, rhizocto-nia cerealis, wheat soil-borne disease and wheat powdery mildew, is efficient, non-toxic and simple to use, and does not cause environmental pollution.

The invention discloses a biocontrol strain for an antagonistic phytopathogen and use thereof. The strain is streptomyces griseoruber Y1B (Streptomyces griseoruber Y1B) CCTCC No:M2013358. The streptomyces griseoruber Y1B is fermented by adopting a GYM culture medium; a fermentation liquor is collected, extracted, and distilled in vacuum, so as to prepare a fermentation crude product. The fermentation crude product has a good antibacterial effect on rhizoctonia solani and watermelon fusarium oxysporum.

The invention discloses a preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth. The bacillus amyloliquefaciens new strain SZ23 is preserved at China General Microbiological Culture Collection Center on July, 25th, 2014, and preservation number is CGMCC No.9481. Morphological characteristics, physiological and biochemical indexes, and 16S rDNA sequencing results of bacillus amyloliquefaciens new strain SZ23 are detailedly disclosed by the invention. It is shown by bacteriostasis experiments that, the fermentation broth of bacillus amyloliquefaciens new strain SZ23 possesses excellent bacteriostatic activity on alternaria kikuchiana, fusarium oxyspirum F sp niveum, fusarium oxysporum Schl.f.sp vasinfectum(Atk.)Snyd.& Hans, alternaria solani, fusarium wilt of cucumber, cotton rhizoctonia solani kuhn, fusarium graminearum schw, and botrytis cirerea Pers et Tris; bacteriostasis rate ranges from 55.6% to 92.2%; and it is confirmed that wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 possesses excellent broad-spectrum antibacterial activity. It is confirmed by practical experiments that control rate of the fermentation broth of new strain SZ23 on alternaria kikuchiana is more than 75%.

The invention discloses a high-yield strain streptomyces lydicus, as well as breeding and fermentation thereof. The fermentation of the high-wield stain streptomyces lydicus E9 CGMCC No.3075 comprises the following steps: (1) preparation of a shake flask, seeds and a fermentation medium; (2) shake flask culture; (2) seed culture; (4) fermentation pot culture: leading a seed culture liquid in a fermentation pot filled with the fermentation medium and culturing for 24-96 hours to obtain a high-wield stain streptomyces lydicus E9 fermentation liquor under the condition of ventilating, stirring, temperature of 25-35 DEG C, and pH of 4-9; and carrying out ultrasonication for 20-40 min. The strain of the invention can generate large quantity of substances with antimycotic activity in the fermentation liquor, and the fermentation liquor has evident inhibiting effect on rhizoctonia solani; and the bacterial inhibition rate of the fermentation liquor which is diluted by 50 times reaches above 97.8% after 48 hour fermentation.

The invention provides a Pseudomonas.chlororaphis subsp.Aurantiaca Pa40, preservation number of which is CGMCCNo.2764. The strain generates HCN, prolease and siderophore relative to antibacterium and has plate board inhibition effect on a majority of tested plant pathogenic fungi and a part of tested plant pathogenic bacteria. Control effect of the biocontrol strain Pa40 on rhizoctonia solani is detected by taking wheat as an indicator plant on the plate, in the greenhouses and in the fields; a result shows that the control effects of the Pa40 on the rhizoctonia solani on the plate bed, in the greenhouse and in the fields are respectively 53.97%, 68.10% and 72.46%; wherein the control effects on the rhizoctonia solani in the greenhouses and fields are higher than that of validamycin.

The invention relates to a bacillus subtilis str.TR21 (hereinafter referred to as 'TR21') strain having broad-spectrum antagonism to plant pathogenic fungi, in particular to the bacillus subtilis TR21 strain and a growth inhibition function thereof on the plant pathogenic fungi. The bacillus subtilis TR21 strain provided by the invention is preserved in China Center for Type Culture Collection (CCTCC) in January 20, 2010 with a preservation number of CCTCC No: M2010019, wherein the China Center for Type Culture Collection is located in Wuhan University, Lojia Mountain, Wuchang, Hubei, China. The strain provided by the invention is proved by obtained gene segments 16S rDNA, gyrA and gyrB and BLAST search and comparison on NCBI to be a novel bacillus subtilis strain having relatively higher flat antagonistic activity to a plurality of plant pathogenic fungi, particularly having high antagonism to various soil borne vascular diseases, such as fusarium oxysporum, rhizoctonia solani, alternaria and the like, and having good prevention and control effects shown in fields.

The invention discloses a gene engineering bacterial strain used for producing phenazine-1-methanamide and its purpose; which is characterized in that knockout of psrA or rpeA gene in Pseudomonas chlororaphis HT66(Pseudomonas chlororaphis HT66) CCTCC NO: M 2013467 genome is carried out. According to the invention, a strain of Pseudomonas chlororaphis is performed with seed culture, fermentation, extraction, concentration and crystallization to obtain phenazine-1-methanamide with purity of more than 96%. After the deletion of psrA and rpeA gene in the bacterium genome, the fermentation output of phenazine-1-methanamide can reach as high as 1800ppm. The agar plate bacteriostasis tests found that phenazine-1-methanamide has obvious inhibition effect on common plant pathogenic fungi such as Rhizoctonia solani, fusarium oxysporum, pythium wltmum and Stevia Septoria, has good stability and activity, and has good application prospect.

The invention relates to bacillus subtilis Pc3 and a use of bacillus subtilis Pc3 in preparation of a fermentation supernatant for preventing and controlling plant pathogenic fungi, and relates to bacilli. The bacillus subtilis Pc3 has a preservation number of CCTCC NO: M2013470. The bacillus subtilis Pc3 can prevent and control plant pathogenic fungi such as gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani and fusaria. A rape sclerotinia sclerotiorum prevention and control pot experiment proves that the bacillus subtilis Pc3 has good biological-prevention and control effects and has prevention and control efficiency of 95.6%. The fermentation supernatant of the bacillus subtilis Pc3 can be prepared by simple processes and has a wide fungus inhibition spectrum and good biological prevention effects.

The invention relates to the technical field of screening of functional microorganisms, and particularly provides a bacillus amyloliquefaciens strain, wherein the collection number is CCTCC NO: M2015075. Growth and propagation of pathogenic bacteria such as alchemilla japonica podosphaera leucotricha, botrytis cinerea, fusarium oxysporum and rhizoctonia solani can be significantly inhibited by bacillus amyloliquefaciens; plant diseases such as strawberry powdery mildew and cucumber gray mold are effectively prevented and treated; and the effect is significant. The bacillus amyloliquefaciens can be applied to prevention and treatment of the plant diseases as an antibiological inoculant, and can also be compounded with other bacilli and moulds with bacteriostatic action to prepare a compound microorganism preparation; the prevention and treatment efficiency on the plant diseases such as powdery mildew, gray mold, brown blotch and fruit rot is 85%-90%; and the application prospect is wide.

The invention discloses a compound microbial fertilizer for antagonism of soil-borne fungal diseases, and a preparation method and an application thereof; the fertilizer takes humic acid as a compound carrier, includes bacillus amyloliquefaciens HFJ-7 with the bacteria content of 1.8*10<8>-2.1*10<8> CFU/g, and also includes bacillus megatherium B3. The bacillus amyloliquefaciens HFJ-7 in the compound microbial fertilizer has the preservation number of CGMCC No.10011. The bacillus amyloliquefaciens HFJ-7 strain can be antagonistic to rhizoctonia solani, fusarium oxysporum, root rot pathogen, cucumber fusarium wilt pathogen, phytophthora capsici leon pathogen and other soil-borne disease pathogenic fungi. After the bacillus amyloliquefaciens HFJ-7 is compounded with the bacillus megatherium B3, the resistance to rhizoctonia solani and pepper root rot pathogen can be significantly improved, a disease state index of pepper seedling root rot disease can be significantly reduced, and the occurrence and spreading of pepper root rot can be effectively prevented and controlled.

The invention discloses a bacillus atrophaeus strain DPPG-28 and an application in crop disease control, and belongs to the field of agricultural biotechnology. The strain is separated from soil of drip irrigation fields with damping off in tomato processing production areas, and has an accession number of CGMCC No. 9394. The strain can be used for biological control of crop pathogens such as rhizoctonia solani, pythium aphanidermatum, alternaria solani, phytophthora capsici, tomato scab fungi, and the like; the control effects on tomato damping off, capsicum early blight, and cotton damping off reach respectively 74.17%, 66.83%, and 70.12%; the strain can significantly improve the crop germination rate, plant height, fresh weight, and dry weight at the seedling stage. The strain of the invention has high-efficient wide-spectrum germicidal activity, and has wide application prospects in biocontrol agents.

The invention relates to the field of biological prevention and treatment of plant diseases, in particular to a bacillus siamensis strain and application thereof. The strain is preserved in CGMCC (China General Microbiological Culture Collection Center); the preservation code is CGMCC NO.12903; the preservation time is August 24, 2016. The strain is used for preventing and treating rhizoctonia solani kuhn; the potential adverse influence of chemical drug used in the current stage on the human environment and the rice safety can be avoided. The strain has the characteristics that the growth speed is high; the sporulation quantity is large; the action spectrum is wide; the stress resistance is great; the plant growth is promoted, and the like. Therefore the strain belongs to a biological prevention and treatment preparation with great application prospects.

The invention discloses bacillus pumilus GBSW19 which is preserved in CGMCC (China General Microbiological Culture Collection Center), the preservation date is 9th September, 2013, and the preservation number is CGMCC No. 8140. The bacillus pumilus GBSW19 can be used for crop straw degradation and simultaneously has functions of inhibiting plant pathogen such as rhizoctonia solani , Xanthamonas oryzae pv.oryzae and the like, so that the growth of rice is promoted, and the bacillus pumilus GBSW19 has a good application prospect.

The invention relates to bacillus amyloliquefaciens, the preservation number of which is CCTCC M 2015299, and which is named as bacillus amyloliquefaciens XK-2. The invention further provides a screening method of the bacillus amyloliquefaciens XK-2 and an application of the bacillus amyloliquefaciens XK-2 in inhibiting watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria as well as a bacillus amyloliquefaciens XK-2 bactericide. The bacillus amyloliquefaciens XK-2 is a watermelon endophytic bacterial which is safe to the human beings, animals and crops, environment-friendly, relatively wide in antibacterial spectrum, has a good inhibition effect on common soil-borne pathogen,watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria, has a significant control effect on watermelon fusarium wilt, has an obvious inhibition effect on the growth of watermelon fusarium oxysporum hypha, slows down the growth of hypha, facilitates the distortion syndrome of the hypha and is wide in application prospect in biological control of plant diseases.

The invention discloses a polysporus trichoderma (Hypocrea pachybasioide) FSR-97 strain, wherein the preservation unit is China General Microbiological Culture Collection Center, the preservation date is 19, September, 2014 and the preservation number is CGMCC No.9691. The strain is cultured on a potato dextrose agar culture medium (PDA) plate at 25 DEG C and rapidly grows, and a monospore is germinated to form a white bacterial colony; the colour on the back surface of the bacterial colony is transited from colourless to yellow. The polysporus trichoderma (Hypocrea pachybasioide) FSR-97 has an effective broad-spectrum antibacterial effect on the main pathogenic bacteria causing the panaxginsen fusarium rot, epidemic disease, sclerotiniose, root rust rot, black spot, rhizoctonia solani, and botrytis. The invention further discloses a plurality of biocontrol mechanisms of the polysporus trichoderma (Hypocrea pachybasioide) FSR-97 for preventing and controlling the plant fungous disease, and an application for preparing a microbial preparation to prevent and control the plant fungous disease.

The invention disclose a pyridine salt compound containing 1,3,4-oxadiazolyl (thiadiazolyl) and a preparation method and application thereof. The compound is of the structure shown in the general formula (I). Based on the 1,3,4- oxadiazole (thiadiazole) thioether (sulfoxide or sulfone) compound, a pyridine base capable of improving the biological activity of the target compound is introduced into the system, and a series of 1,3,4- oxadiazole (thiadiazole) oxygen ether, thioether, sulfoxide or sulfone amphipathic molecules containing pyridine salt are synthesized. It is found that the compound has the good restraining effect on pathogenic bacteria and fungi such as Xanthomonas oryzae pv. oryzae, Ralstonia solanacearum, Xanthomonas axonopodis pv. citri, Gibberella zeae, Phytophythora infestans, Rhizoctonia solani, Borrytis cinerea, Fusarium oxysporum and Phytophythora infestans, and provides an important scientific foundation for new pesticide research, development and creation.

The invention provides bacillus subtilis and preparation and application of a microbial inoculum thereof. The invention mainly relates to a Bacillus subtilis Y-1 of which the preservation number is CGMCC No.6539, and also relates to the microbial inoculum which is prepared from the bacillus subtilis, and application of the microbial inoculum. The Y-1 strain can be colonized on an apple tree, a polar tree, a cucumber and the rhizosphere of capsicums and in an apple tree, and has efficient and broad-spectrum antibacterial activity on fusarium, rhizoctonia solani, pestallozzia theae, phytophthora and the like; the microbial inoculum of the bacillus subtilis can be used for controlling diseases and continuous cropping obstacles of an apple tree, a polar tree, a cucumber and capsicums caused by those disease fungi. The preparation method of the microbial inoculum is simple in process, short in fermentation cycle, low in cost, and favorable for industrial production.

The invention relates to the field of biotechnology, in particular to application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control. In the invention, endophytic fungus of camphor trees is separated from camphor tree living bodies collected from Tianmu mountain in Hangzhou, Zhejiang province by adopting endophytic fungus separation technology, and isnamed alternaria alternata 31 through microbial taxonomy identification, and the strain is stored in China General Microbiological Culture Collector Center (CGMCCC) on December 31, 2010, and the collection register number is CGMCC No.4508. The invention has a most obvious characteristic that the strain liquid can be fermented to produce active metabolic products which have inhibiting effect on pathogenic bacteria of plant fungus diseases such as Rhizoctonia solani, Fusarium oxysporium, Botrytis cinerea and the like, thus alternaria alternate is an important microbial resource in agriculture.

The invention relates to burkholderia cepacia, and a cultivation method and an application thereof, and belongs to the field of plant protection (microbial pesticide). A preservation number of the burkholderia cepacia is CGCC No. 5384. According to the cultivation method, fermentation conditions are as follows: an LB culture medium is used; liquid volume is 50 mL; 1 mL of 2-day old seed strain is added; and the strain is cultured for 20-24 h at a temperature of 25-30 DEG C and at a rotation speed being 200 r/min. The burkholderia cepacia has significant inhibition effects for rhizoctonia solani, pyricularia oryzae, collectotrichum musae, fusarium oxysporum f. sp. cubense, gibberella zeae, peronophythora litchi, ceratocystis adiposum, fulvia fulva, xanthomonas oryzae pv. oryzae, xanthomonas oryzae pv. oryzicola and the like, can be used for biological control of plant pathogenic bacteria, has the advantages of low toxicity, easy degradation, environment friendliness and the like, and has wide application prospects.

The invention discloses Bacillus subtilis strain HMB26553 (Bacillussubtilis), the strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCCNo.13211. The present invention also discloses a microbial agent produced by use of the strain and application thereof in the prevention and control of plant diseases such as cotton damping-off and the like. The Bacillus subtilis HMB26553 has high control efficiency on the cotton damping-off, the average control efficiency is above 90%; the microbial agent has good effect perdurability, and has no environmental pollution problems; in addition, the HMB26553 has a wide antibacterial spectrum, and has a good inhibitory effect on cotton rhizoctonia solani kuh, cotton verticillium dahlia, cotton Fusarium oxysporum, tomato botrytis cinerea and cucumber Corynespora cassiicola.

The invention discloses a methylotroph bacillus and application thereof to preparation of a plant pathogenic bacterium sterilization agent. The methylotroph bacillus CGMCC No.13541 has the antagonism effect on various phytopathogens such as botrytis cinereal, fusarium graminearum and rhizoctonia solani, and belongs to an environment-friendly antagonistic strain with the obvious plant disease prevention and control effect; the development and application prospects are good. Culture liquid obtained through culture by a beef extract liquid culture medium can be used for preventing and controlling plant diseases. The bacteriostasis rate on the botrytis cinereal is as high as 84 percent; the value is about 8 percent higher than that of ordinary biocontrol bacteria; the foundation is laid for the novel disease prevention microbe resource development and utilization. The resistance capability is high; the use range is wide; the growth speed is high; fast and great-amount multiplication can be realized; the popularization and application of a biological prevention and control preparation in the field are convenient.

The invention discloses a method for quickly identifying resistance to banded sclerotial blight in rice seedling stage, comprising the following steps: activating and culturing the strain of rhizoctonia solani so as to obtain mycelium; overgrowing the mycelium on strip medical absorbent cottons through a culture medium so as to form absorbent cottons with bacteria and good moisturizing performance; soaking seeds of the rice variety (series) to be identified to accelerate germination at the temperate of 30 DEG C after disinfecting, and then seeding in the soil inside the box body, and culturing for 3-4 leaf ages; intertwining the straw base of the seedling with the strip absorbent cotton with rhizoctorzia solani, then covering a plastic cover (taken off 24 hours later) on the seedlings, and carrying out normal growth management of the seedlings; and identifying the resistance to banded sclerotial blight in the rice seedling stage according to seedling-straw ratio 5-7 days after inoculation.

The invention discloses a Brevibacillus laterosporus strain N106-2 of which the collection number is CGMCC No.8482. The strain has strong indoor flat inhibiting actions on multiple plant pathogenic fungi (fussarium wilt of tomato, root rot of chili, gray mold of tomato, Rhizoctonia solani, black spot of cabbage, anthracnose of pear and black rot of pear) and plant pathogenic bacteria (bacterial wilt of tomato, fire blight of pear and streak of rice); and the strain can secrete proteinase, cellulase and surfactant antimicrobial related substances with hemolytic activity. The Brevibacillus laterosporus can be prepared into a biocontrol aqua; the biocontrol aqua has certain preventing and treating effects on fussarium wilt of tomato, fusarium rot of chili, anthracnose of pome and eyespot of pome. The Brevibacillus laterosporus N106-2 has the advantages of no toxicity, no pathogenicity and high safety for human and livestock, is environment-friendly, is a useful resource for development of microbial pesticides, and has potential application prospects.

The invention discloses an oleanolic acid oxime ester derivate compound, a preparation method and an application thereof. The structural formula of the compound is shown in formula I, and in the formula I, R refers to furyl, pyridyl, 1-menaphthyl, 4-chlorophenoxy methyl or phenyl with 1 to 2 substitutional groups; and the substitutional groups in phenyl with 1 to 2 substitutional groups are halogen (such as fluorine, chlorine, bromine and iodine) or nitryl. The preparation method comprises the following steps: 1) performing the esterification reaction between oleanolic oxime subject to benzylation protection and substituted carboxylic acids to prepare an oxime ester compound; and 2) removing benzylation protection on the oxime ester compound by Pd/C-H2 to prepare the compound in the formula I. The bactericidal activity test result shows that CAU2012-A has a favorable effect of inhibiting growth of sclerotinia scleotiorum, phytophthora melongenae, botrytis cintrea, pyricularia grisea, rhizoctonia solani and cotton fusarium wilt.

The invention discloses a method for promoting orchid to grow and preventing diseases, and relates to a method for preventing the diseases and promoting orchid branches to grow and prolonging a flowering phase. The method disclosed by the invention comprises the following steps of uniformly mixing a solid state culture and a culture medium into a basin according to the mass ratio of 1 to 50 in orchid plant transplantation, wherein the solid state culture has the spore concentration s 1*10<7>-1*10<8> spore/g and prepared from Trichoderma harzianum T216, and planting an orchid plant, wherein the solid state culture can induce the resistance of the orchid plant to the fungoid diseases such as southern blight, rhizoctonia solani and anthracnose and bacterial soft rot caused by Erwinia carotovara during orchid growth, the plant morbidity is reduced, the disease index is reduced, the orchid is promoted to grow in advance, and the number of blades of the orchid are increased. In addition, the method disclosed by the invention has the advantages that a liquid state culture of the Trichoderma harzianum T216 is regulated into 1*10<5>-1*10<7> spore/mL when the orchid peeps a bud, and the spray treatment is carried out on the orchid plant, so that the branch number of the orchid is increased, and the flowering phase of the orchid is prolonged.