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Application of amaranthus in plant disease prevention and treatment

A technology of plant diseases and plant pathogens, applied in the fields of application, plant growth regulators, botany equipment and methods, etc., can solve the problems of pesticide residues, destruction of ecological balance, pollution of the environment, etc.

Active Publication Date: 2013-08-14
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of production, chemical pesticides were mainly used for control in the past. However, due to problems such as pesticide residues, environmental pollution, and ecological balance damage, Jinggangmycin is mainly used now. However, long-term, large-scale, single-use Jinggangmycin will cause sheath blight Bacteria develop drug resistance, and the European Union has also listed Jinggangmycin as a dangerous agent

Method used

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  • Application of amaranthus in plant disease prevention and treatment
  • Application of amaranthus in plant disease prevention and treatment
  • Application of amaranthus in plant disease prevention and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of Example 1 Amaranth Leaf Extract (illustrated with ethyl acetate as the extraction solvent)

[0021] Take amaranth leaves, wash them, dry them in the sun until the water content is 3%, and at room temperature, grind them into 100-120 mesh fine powder through a high-speed pulverizer at a speed of 8000-12000r / min to obtain amaranth leaf powder; take 10g of amaranth leaf powder , add analytically pure ethyl acetate 180mL, shake on a shaker at 90-120r / min at 20-25°C for 30h, filter, and collect the filtrate; Shake at -120r / min for 30h on a shaker, filter, and collect the filtrate; repeat this for the filter residue once, and combine the filtrate three times to obtain the extract; put the obtained filtrate into a rotary concentration evaporator, The temperature of the water bath is 40-43°C, the condensation temperature is 8-10°C, and the rotation speed is 80-120r / min, and concentrated to a paste to obtain the extract.

Embodiment 2

[0022] Example 2 The inhibitory effect of amaranth leaf extract on watermelon bacterial fruit spot and citrus canker

[0023] Take 1g of amaranth leaf extract and add 5-10mL of analytically pure ethyl acetate to dissolve to obtain the active substance to be tested. The antibacterial effect was detected by plate punching method. The watermelon bacterial fruit blotch suspension and the citrus canker sore suspension (1×10 8 cfu / mL) 60 μL was evenly spread on the NA plate, and then a hole was made on the central side of the plate (φ=5mm), and the active substance to be tested was injected, and ethyl acetate was injected symmetrically on the other side of the plate as a control. Incubate 50 μL of the well at 28°C for 24-48 hours, and observe the antibacterial situation. Each treatment was repeated 3 times. It can be seen by observation that the extract of amaranth leaves has inhibitory effects on watermelon bacterial fruit spot and citrus canker, see figure 1 and figure 2 , w...

Embodiment 3

[0024] Example 3 The inhibitory effect of amaranth leaf extract on rice sheath blight bacteria

[0025] Take 1g of amaranth leaf extract and add 5-10mL of analytically pure ethyl acetate to dissolve to obtain the active substance to be tested. The inhibitory activity of the extracts against rice sheath blight was detected by punching method. Put the rice sheath blight bacteria in the center of the PDA plate, punch holes at 1cm on both sides, inject the active substance to be tested on one side, and inject ethyl acetate on the other side as a control, repeat each treatment 3 times, and place at 25-28°C In the incubator, cultivate for 48 hours, and observe whether there is any antibacterial phenomenon. It can be known by observation that the extract of amaranth has inhibitory effect on rice sheath blight bacteria, see image 3 , while the control ethyl acetate had no inhibitory effect on sheath blight bacteria. It shows that there are antibacterial active substances in the le...

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Abstract

The invention provides an application of amaranthus in plant disease prevention and treatment, wherein amaranthus leaves are taken as the raw material and extracted by an organic solvent to obtain an extract, acidovorax avenae subsp.citrulli, xanthomonas axonopodis pv. Citri and rhizoctonia solani are selected as sites of action, and research on indoor bacteriostasis effect and in-vitro and pot culture control effects is performed to find that the amaranthus leaf extract has an inhibition effect on acidovorax avenae subsp.citrulli, xanthomonas axonopodis pv. Citri and rhizoctonia solani; and the in-vitro and pot culture tests also indicate that the amaranthus leaf extract has certain prevention and treatment effects on acidovorax avenae subsp.citrulli, xanthomonas axonopodis pv. Citri and rhizoctonia solani; and all the facts show that the amaranthus has prevention and treatment effects on the plant diseases in addition to edible and medical values. The research discovers and expands the functions of the vegetable, and simultaneously provides a new train of thought and a new way for studying and developing biopesticide for preventing and treating the plant diseases.

Description

technical field [0001] The invention belongs to the field of vegetable application, and in particular relates to the prevention and control effect of amaranth on plant diseases, more specifically, relates to the prevention and control effect of amaranth on watermelon bacterial fruit spot, citrus canker and rice sheath blight. Background technique [0002] Amaranth is a herbaceous plant belonging to the genus Amaranth in the Amaranthaceae family. It is mainly eaten, but its medical functions of anti-oxidation, anti-cancer and liver protection are constantly being explored. In recent years, with the deepening of research on amaranth, it has been found that amaranth extract has a strong effect on food saprophytic bacteria such as: Salmonella spp., E. coli, Staphylococcus aureus (S. aureus), Listeria (L.monocytogenes) etc. have inhibitory effect. The seed extract has inhibitory effect on fungi such as Geotrichum candidum, Fusrium solani, Penicillium chryso-genum and Aspergillus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01P3/00A01N65/08A01P1/00
Inventor 廖晓兰张亚苏品黄璜刘双清夏日照仇佳伟尹伟
Owner HUNAN AGRICULTURAL UNIV
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