The invention relates to an RPA detection primer, a probe and a detection method of cucurbit
bacterial fruit blotch, the RPA detection primer comprises an RPA primer pair Ac-F1 and Ac-R1 of cucurbit
bacterial fruit blotch and a detection probe Ac-nfo, the amplification product size of the RPA primer pair Ac-F1 and Ac-R1 is 342bp, the 5'terminal of the primer Ac-R1 is labeled with
biotin, the 5 'terminal of the primer Ac-R1 is labeled with
biotin, and the 5' terminal of the detection probe Ac-nfo is labeled with
biotin. The 5'
tail end of the detection probe Ac-nfo is marked with FAM, the 3 '
tail end of the detection probe Ac-nfo is marked with C3Spacer, and the position, 32 bp away from the 5'
tail end, of the probe is modified with dSpacer; the RPA primer group is high in detection sensitivity, and the
detection limit reaches 2.5 * 10 < 4 > cfu / mL; the invention provides a detection method for detecting the melon
bacterial fruit blotch based on RPA combined with
lateral flow test paper, establishes a rapid isothermal
visual detection method for the melon bacterial fruit blotch, can complete sample detection within 15-20 min, realizes rapid diagnosis, identification and detection of the melon bacterial fruit blotch in a constant-temperature environment, and has a wide application prospect. The kit has the advantages of high sensitivity, good specificity and the like, and provides
technical support for
quarantine, detection, prevention and control of the
disease.