39 results about "Bacterial fruit blotch" patented technology

The invention relates to a bio-control strain JC65 for preventing and controlling bacterial fruit blotch of watermelon, and belongs to the technical field of crop disease prevention and control. The bacteria are Bacillus sp.; and the bacteria are subjected to shaking culture for 20 to 24 hours in load balance (LB) culture solution at the temperature of 30 DEG C and at the revolution speed of 180rpm, then the solution is centrifuged for 10 minutes at the revolution speed of 6,000rpm, the wet bacteria and the bacteria preservation solution are prepared into bactericide in a ratio of 1:40 (g/ml), and the total concentration of living bacteria in the finished product is 1*10<9> to 1*10<12>cfu/ml. The bactericide is diluted to the concentration of 1*10<8>cfu/ml in use, the prevention effect on the bacterial fruit blotch of watermelon is 50 to 90 percent, and the yield increasing effect on a sick field is 10 to 200 percent. The growth promoting effect on the disease-free plants can reach 10to 200 percent, and the yield increasing effect on the disease-free field is 5 to 20 percent. The bactericide can be blended with water for spraying or made into compost for use, and can also be diluted for irrigating roots during transplanting.

The invention belongs to the technical field of crop disease prevention and treatment and discloses a bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof. The bio-control bacteria strain 1JN2 is named as Bacillus axarquiensis in classification, and is filed at General Microbiological Center of China General Microbiological Culture Collection Committee with CGMCC No.5731 on January 16, 2012. The bio-control bacteria strain 1JN2 is cultured in LB (luria-bertani) broth at the temperature of 28 DEG C by vibration of 180rpm for 12-16 hours, centrifuged by 6000rpm for 10 minutes, diluted by sterilization water to prepare bactericide, and total concentration of viable bacteria in the finished bactericide is 1X10<9>-1X10<10>CFU/ml. The experiment results in a greenhouse indicates that the bio-control bactericide with the 1JN2 has evident growth acceleration effect to watermelons, biomass live weight is increased to 55.92% and evident prevention effect to disease of fruit blotches of the watermelons, disease incidence can be reduced by 50.13%.

The invention relates to a melon bacterial fruit blotch immune colloidal gold rapid detection test strip. The test strip comprises a sample pad, a colloidal gold sample closely connected with the sample pad, a cellulose membrane arranged on the colloidal gold pad and closely connected with the colloidal gold pad, and a water absorption pad closely connected with the other end of the cellulose membrane, one end of the cellulose membrane away from the colloidal gold pad is provided with a quality control line, and the cellulose membrane between the quality control line and the colloidal gold pad is provided with a detection line, wherein the colloidal gold pad is provided with a monoclonal antibody coupling with colloidal gold, the monoclonal antibody is secreted by a hybridoma cell strain with the preservation number of CGMCC NO.10413, and the detection line is formed by the monoclonal antibody. The fruit blotch immune colloidal gold rapid detection test strip adopts the colloidal gold pad and the detection lime to be respectively sprayed with the new screened monoclonal antibody and has high detection specificity and sensitivity on fruit blotch. The invention also relates to monoclonal antibody for the test strip, and the hybridoma cell strain thereof.

The invention relates to an extraction method of essential oil of artemisia argyi. The extraction method comprises the following steps: obtaining plants of artemisia argyi from saline land, drying plants of artemisia argyi at a temperature of 40 DEG C until the weight is constant, grinding and sieving the plants to obtain artemisia argyi powder; adding an artemisia argyi powder sample into a supercritical CO2 extraction apparatus, starting a water bath until the extraction temperature reaches 35 DEG C, carrying out extraction for 90 minutes under following conditions: extraction pressure: 20 MPa, CO2 flowing speed: 20 g/min, and temperature of separation kettle: 30 DEG C; after extraction, washing out the extract by anhydrous ethanol, carrying out centrifugation to remove wax, removing water by anhydrous sodium sulfate; and subjecting filtrate to reduced pressure distillation at a temperature of 30 DEG C to recover ethanol and obtain wax free volatile oil of artemisia argyi. The provided method can quickly and efficiently extract essential oil of artemisia argyi. The essential oil comprises many components. The aromatic components of essential oil of artemisia argyi can be well maintained. The essential oil of artemisia argyi has a good effect on inhibiting xanthomonas oryzae, ralstonia solanacearum, pathogenic bacteria that causes cucumber bacterial angular leaf spot, pathogenic bacteria that causes bacterial fruit blotch of cucurbit, potato rhizoctonia solani, soft-rot fungi of cabbage, and the like.

The invention relates to inspection and quarantine of bacterial fruit blotch of melons, and particularly relates to a PCR (Polymerase Chain Reaction) primer and a method for identifying different subgroups of bacterial fruit blotch of melons. A PCR primer group for identifying different subgroups of bacterial fruit blotch of melons, which is provided by the invention, comprises a forward primer BFB and reverse primers BFB1 and BFB2, and nucleotide sequences of the primers are as follows: BFB: 5'ATGAAGCGTACTGTTCAG-3'; BFB1: 5'ACCATCGATATTAGCATC-3'; BFB2: 5'-TTAAGGAGCAAACGTGC-3'.

The invention discloses application of chitosan in prevention and control of bacterial fruit blotch of plants, wherein the deacetylation degree of the chitosan is more than 85%. The plants are melon plants. The application comprises the steps of placing plant seeds in a chitosan solution to be soaked for 3-5 hours or using the chitosan solution to perform spraying treatment to leaves of the plants at a trefoil stage. The chitosan uses as a main raw material, the prepared and obtained chitosan solution can effectively inhibit the activity of the bacterial fruit blotch and prevent and control the bacterial fruit blotch of the plants. The chitosan solution with certain concentration further can effectively promote growth of watermelon seedlings. The raw material is easily obtained, the process is simple, the operation is convenient, and the chitosan is low in toxicity and harm and good in preventing and controlling effect on the bacterial fruit blotch of melon plants. In addition, the chitosan can further promote the growth of plant seedlings to some extent and is suitable for actual production and easy to popularize and apply.

The invention discloses a protein chip for rapid quantitative determination of bacterial fruit blotch of watermelon and a preparation method thereof. The protein chip for rapid quantitative determination of bacterial fruit blotch of watermelon is prepared by fixing at least one capture antibody of bacterial fruit blotch of watermelon on a chip substrate. Cellulose nitrate membrane is used as the chip substrate, specific antibodies are used for capturing bacterial fruit blotch of watermelon, IgG labeled with alkaline phosphatase is used as a positive control, a point sample buffer is used as a negative control, and the detection result can be obtained by direct naked eye judgement or quantitative determination using grey analysis. Compared with traditional ELISA, the protein chip provided by the invention can attain the same detection capability with ELISA by using a twelfth of amount of the capture antibody and an eighth of detection time, with simple operation and long-term preservation of detection results, and the protein chip can be used for rapid self-detection of bacterial fruit blotch of watermelon on the planting line with a wide popularization prospect.

The invention belongs to the field of disinfection and control of plant seed-borne diseases, particularly relates to disinfection, prevention and treatment of watermelon bacterial fruit blotch, and discloses a novel compound disinfectant.The disinfectant comprises chlorine dioxide (ClO2) mainly having a disinfection effect, sodium hypochlorite (NaClO) mainly having a germination promoting effect, and potassium iodate (KIO3) mainly having an effect of stabilizer By means of the disinfectant, detected watermelon seeds with bacterial fruit blotch bacteria and artificial simulating watermelon seeds with bacterial fruit blotch bacteria are processed, the germination rate and germination potential of seeds are promoted, and the disinfection effect is remarkable and is obviously better than that of a reported existing bacterial fruit blotch disinfectant and a reported existing disinfecting method.The disinfection method can easily, rapidly and thoroughly kill bacterial fruit blotch bacteria carried by watermelon seeds, is easy to operate, free of toxin and pollution and has huge actual application value.

The invention belongs to the field of crop breeding, and specially relates to a seed processing agent for preventing cucurbit bacterial fruit blotch. The processing agent comprises the following raw materials, the mass to volume to volume ratio of which is 1-5 of copper oxychloride to 0.2-2 of acetic acid to 500-3000 of water. Copper oxychloride comes from wettable powder of copper oxychloride, preferably the wettable powder of 50% of copper oxychloride. The acetic acid comes from vinegar, preferably white vinegar. According to the seed processing agent, copper oxychloride and acetic acid are taken as effective components and have a synergistic effect, so that the bactericidal rate is improved. The seed processing agent is suitable for dry seeds and wet seeds, and the bacteria carrying rate of cucurbit seeds is reduced.

The invention discloses a watermelon seed disinfection technology for controlling the occurrence of bacterial fruit blotch. The technology comprises the step of disinfecting seeds with a CuSO4 water solution. A result shows that the treated seeds can effectively kill bacterial fruit blotch bacteria carried by the seeds so that the occurrence of field fruit blotch can be reduced after the seeds are planted. Meanwhile, the treatment is triggered by a solid base material so that the germination speed and the uniformity of diploid watermelon seeds can be effectively improved; and the germination obstacle of triploid watermelon seeds can be alleviated and the final germination rate is improved.

The invention discloses a compound for preventing and treating plant pathogenic bacteria and application thereof. The compound is 4-allyl catechol, tests prove that the 4-allyl catechol has antibacterial activity, and when the test concentration of the compound is 1000 [mu] mol/L, the compound has a good antibacterial effect on xanthomonas campestris pv, xanthomonas oryzae pv. oryzicola, xanthomonas citri, bacterial fruit blotch of muskmelon, bacterial wilt of tomato, bacterial angular leaf spot of strawberry, bacterial angular leaf spot of mango, black rot of cabbage and soft rot of potato, and the inhibition rate is 90% or above; meanwhile, in-vivo test results show that the compound has a good inhibition effect on pathogenicity of potato soft rot fungi, and also has a good prevention and treatment effect on mango bacterial angular leaf spot bacteria and melon bacterial fruit blotch bacteria. The results show that the 4-allyl catechol has a broad-spectrum bactericidal effect on plantpathogenic bacteria. Therefore, a foundation is laid for further creating a novel pollution-free botanical pesticide by utilizing a unique plant, namely South China pepper, in China.

The invention discloses a novel method for preventing and treating muskmelon bacterial fruit blotch. The novel method is characterized in that the purpose of preventing and treating muskmelon bacterial fruit blotch can be realized through processes such as seed sterilization, soil sterilization, crop rotation, ridge culture, reasonable watering, timely control of injurious insects in a shed room, bagging treatment of plastic bags and chemical control. This way is convenient to operate, can effectively increase the yield of muskmelons and can effectively improve the quality of the muskmelons, and is suitable for preventing and treating muskmelon bacterial fruit blotch.

A new method for preventing and treating bacterial fruit spot of melon, which is characterized in that through the process of seed disinfection, soil disinfection, crop rotation, ridge cultivation, rational watering, timely prevention and control of greenhouse pests, plastic bag bagging treatment, chemical control, etc., The purpose of preventing and controlling the bacterial fruit spot disease of the melon can be realized. This method is not only easy to operate, but also can effectively improve the yield and quality of the melon, and is suitable for the prevention and treatment of the bacterial fruit spot disease of the melon.

The invention relates to a watermelon bacterial fruit blotch controlling novel technology. According to the invention, a purpose of controlling watermelon bacterial fruit blotch can be realized through the processes of cleaning diseased plant residues in the field, adopting crop rotation, selecting disease-resistant or disease-tolerant varieties, reasonably fertilizing, adopting chemical agent control, and the like. The technology has good effect in improving watermelon quality and yield, and is suitable to be used in controlling watermelon bacterial fruit blotch.

The invention discloses a method for detecting bacterial fruit blotch and xanthomonas malvacearum of melons simultaneously and a special kit thereof. The method comprises the following steps: adoptinga sample to be detected as a template, adopting the kit to carry out PCR (Polymerase Chain Reaction) amplification and obtaining reaction products; reading fluorescence signals of the reaction products, then judging whether the sample to be detected contains the bacterial fruit blotch and/or the xanthomonas malvacearum of the melons. The kit comprises a primer pair combination. The nucleotide sequences of 4 primers forming the primer pair combination are shown as a sequence 3 to a sequence 6 in a sequence table. Proved by experiments, on an LGC molecular detection platform, by adoption of thekit provided by the invention, whether the bacterial fruit blotch and/or the xanthomonas malvacearum of the melons are/is carried on melon seeds can be detected rapidly by high flux, so that the method and the kit can be used as a prevention technology for preventing and controlling circulation of infected seeds, also can be used as a disease monitoring and early-warning technology for the fieldplanting stage and provide a fast and accurate detection method for agricultural production and port quarantine.

The invention discloses a method and a special seed fungicide for preventing and controlling bacterial fruit blotch of melons. The method comprises the step of soaking seeds with zinc pyrithione. Experiments show that zinc pyrithione can effectively remove acidovorax citrulli from the outside and inside of the seeds of cucurbitaceae crops (such as watermelons and muskmelons), and can be absorbed by seedlings during seed germination, so that the acidovorax citrulli spread between the seeds and the seedlings is reduced, the emergence incidence rate of the seeds carrying the acidovorax citrulli is effectively inhibited, and the purpose of preventing and controlling the bacterial fruit blotch of the melons is achieved; furthermore, the zinc pyrithione does not affect seed germination and seedling growth; the zinc pyrithione has no significant effect on the growth of plant growth promoting rhizosphere bacteria (such as pseudomonas fluorescens and bacillus subtilis) while inhibiting the growth of pathogens (such as bacterial canker of tomato and xanthomonas campestris pv.campertris). The zinc pyrithione can be used as a seed bactericide for production and promotion. The method and the special seed fungicide have an important application value.

The present invention discloses a method for preventing and curing bacterial fruit blotch of melon in a solar greenhouse. Preventing and curing of bacterial diseases are strengthened after the melon is planted, when freshly born melon grows to 3 to 5 cm, the melon is dipped with 150 to 200 mg/kg of a potassium phosphite aqueous solution for the first time, and within 8 to 10 days after melon dipping for the first time, the melon is dipped with 350 to 450 times of an aqueous solution of 3% of ZhongShengmycin wettable powder for the second time. According to the method for preventing and curingbacterial fruit blotch of the melon in the solar greenhouse, bacterial fruit blotch on melon fruits in the solar greenhouse can be effectively prevented.

The invention relates to an RPA detection primer, a probe and a detection method of cucurbit bacterial fruit blotch, the RPA detection primer comprises an RPA primer pair Ac-F1 and Ac-R1 of cucurbit bacterial fruit blotch and a detection probe Ac-nfo, the amplification product size of the RPA primer pair Ac-F1 and Ac-R1 is 342bp, the 5'terminal of the primer Ac-R1 is labeled with biotin, the 5 'terminal of the primer Ac-R1 is labeled with biotin, and the 5' terminal of the detection probe Ac-nfo is labeled with biotin. The 5'tail end of the detection probe Ac-nfo is marked with FAM, the 3 'tail end of the detection probe Ac-nfo is marked with C3Spacer, and the position, 32 bp away from the 5' tail end, of the probe is modified with dSpacer; the RPA primer group is high in detection sensitivity, and the detection limit reaches 2.5 * 10 < 4 > cfu/mL; the invention provides a detection method for detecting the melon bacterial fruit blotch based on RPA combined with lateral flow test paper, establishes a rapid isothermal visual detection method for the melon bacterial fruit blotch, can complete sample detection within 15-20 min, realizes rapid diagnosis, identification and detection of the melon bacterial fruit blotch in a constant-temperature environment, and has a wide application prospect. The kit has the advantages of high sensitivity, good specificity and the like, and provides technical support for quarantine, detection, prevention and control of the disease.