Method and special seed fungicide for preventing and controlling bacterial fruit blotch of melons
A fruit spot disease and bacterial technology, applied in the field of prevention and control of bacterial fruit spot disease of melons and its special seed fungicide, can solve the problems of limited seedling disease control effect, difficulty in seed treatment, and influence on germination Rate and other issues
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[0058] Example 1. Screening of small molecular compounds and their application in the prevention and control of bacterial fruit spot of melons
[0059] 1. Pathogenicity experiment of watermelon acidophilus strains
[0060] 1. The strain preservation solutions of 12 watermelon acidophilus bacteria shown in Table 1 (bacterial strains are all stored in 15% (v / v) glycerol aqueous solution, -80 ° C) are inoculated in PF medium or NBY medium respectively, 28 ° C Culture for 2-3d (purpose for activation).
[0061] 2. After completing step 1, respectively expand the activated watermelon acidophilus to obtain a concentration of 10 8 CFU / mL bacterial suspension.
[0062] 3, get watermelon seed, conventional planting, obtain the watermelon seedling that grows two true leaves. Get melon seeds, conventional planting, obtain the melon seedling that grows two true leaves.
[0063] 4. Dip the sterile dissecting scissors (obtained by heating the dissecting scissors on a flame or soaking th...
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