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Compound for preventing and treating plant pathogenic bacteria and application thereof

A technology of phytopathogenic bacteria and compounds, which is applied in the field of compounds for the prevention and treatment of phytopathogenic bacteria, can solve the problems of no reports on the inhibitory effect of 4-allyl catechol, and achieve good control effects

Active Publication Date: 2020-12-15
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After retrieval, the active ingredient of South China pepper, protocatechualdehyde, is used in medicine to fight bacteria. At present, there is no report on the agricultural activity of 4-allyl catechol at home and abroad, and there is no 4-allyl catechin Report on Inhibitory Effect of Phenols on Plant Pathogenic Bacteria

Method used

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  • Compound for preventing and treating plant pathogenic bacteria and application thereof
  • Compound for preventing and treating plant pathogenic bacteria and application thereof
  • Compound for preventing and treating plant pathogenic bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation and identification of 4-allyl catechol

[0027] The preparation method of 4-allyl catechol comprises the following steps:

[0028] S1: take the branches and leaves of South China pepper and dry them naturally in the shade, then crush them through a 40-mesh sieve to obtain 2 kg of dry powder, and use 95% ethanol solution with a volume concentration (the mass ratio of dry powder to 95% ethanol is 1:10) for the first time. After cold soaking for 7 days, filter, Obtain the first filtrate and filter residue; filter residue continues to use volume concentration 95% ethanol solution for the second time cold leaching extraction (the mass ratio of filter residue and 95% ethanol is 1:10), extracts altogether 3~5 times, until filtrate is colorless, obtains For the second filtrate, the first filtrate and the second filtrate were combined, and the filtrate was concentrated to dryness in a rotary thin film evaporator under reduced pressure to obtain 302.14 g of ...

Embodiment 2

[0039] The difference between this example and embodiment 1 is:

[0040] In step S1, the volume concentration of the ethanol solution is 90%; the time for the first cold soak is 6 days; the mass ratio of the dry powder to the ethanol solution is 1:9; the mass ratio of the filter residue to the ethanol solution is 1:9.

[0041] In step S3, the volume ratio of petroleum ether-ethyl acetate is 3:1;

[0042] In step S4, the volume ratio of petroleum ether-ethyl acetate is 9:1.

[0043] The experimental result of this example is identical with embodiment 1.

Embodiment 3

[0044] Example 3 Determination of the inhibitory activity of 4-allyl catechol to phytopathogenic bacteria

[0045]In the research on the agricultural fungicidal active substances of South China pepper, the inventors found that 4-allyl catechol has a control effect on plant pathogenic bacteria by using the activity tracking method. Take the following experiments as an example to verify the role of 4-allyl catechol in preventing and controlling plant diseases.

[0046] 1. The strains to be tested

[0047] Xanthomonas oryzae, Xanthomonas oryzae, Xanthomonas citri subsp.citri, Acidovorax citrulli, Ralstonia solanacearum Xanthomonas fragariae, Xanthomonas campestris, Xanthomonas campestris, Pectobacterium carotovorum subsp. carotovorum, Pectobacterium carotovorum subsp. brasiliense, Xanthomonas campestris (Xanthomonas compestrispv. mangiferaeindicae). The above strains were all provided by the Pesticide Chemical Biology and Application Research Group of the Institute of Environm...

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Abstract

The invention discloses a compound for preventing and treating plant pathogenic bacteria and application thereof. The compound is 4-allyl catechol, tests prove that the 4-allyl catechol has antibacterial activity, and when the test concentration of the compound is 1000 [mu] mol / L, the compound has a good antibacterial effect on xanthomonas campestris pv, xanthomonas oryzae pv. oryzicola, xanthomonas citri, bacterial fruit blotch of muskmelon, bacterial wilt of tomato, bacterial angular leaf spot of strawberry, bacterial angular leaf spot of mango, black rot of cabbage and soft rot of potato, and the inhibition rate is 90% or above; meanwhile, in-vivo test results show that the compound has a good inhibition effect on pathogenicity of potato soft rot fungi, and also has a good prevention and treatment effect on mango bacterial angular leaf spot bacteria and melon bacterial fruit blotch bacteria. The results show that the 4-allyl catechol has a broad-spectrum bactericidal effect on plantpathogenic bacteria. Therefore, a foundation is laid for further creating a novel pollution-free botanical pesticide by utilizing a unique plant, namely South China pepper, in China.

Description

technical field [0001] The invention relates to the technical field of plant disease control, in particular to a compound for preventing and controlling plant pathogenic bacteria and its application. Background technique [0002] South China pepper (Piper austrosinense) is a woody climbing vine of the family Piperaceae, mainly distributed in Guangxi, Guangdong, Hainan and coastal islands. According to the records of "Chinese Materia Medica", South China pepper has the effect of reducing swelling and relieving pain, and is mainly used for toothache and bruises. 4-allyl catechol is a component with good bactericidal activity isolated by the project team from South China pepper, a plant of the Piperaceae family, using the biological activity tracking method. Its chemical structure is shown in formula I. [0003] [0004] After searching, protocatechualdehyde, the active ingredient of South China pepper, is used in medicine to fight bacteria. At present, there is no report o...

Claims

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Application Information

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IPC IPC(8): C07C39/19C07C37/82C07C37/70C07C37/72A01N31/16A01P1/00A01P3/00
CPCC07C39/19C07C37/82C07C37/70C07C37/72C07C37/685A01N31/16Y02A50/30
Inventor 张静王琴朱发娣辜柳霜叶火春闫超冯岗
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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