Bacterial fruit blotch seed disinfectant and application thereof

A technology of fruit spot bacteria and fungicides, which is applied in the field of sterilization and control of plant seed-borne diseases, can solve the problems of not being able to completely kill the germs and affect the germination rate of seeds, and achieve the best comprehensive effect of sterilization and control, and the sterilization effect is thorough , the effect of promoting germination potential

Active Publication Date: 2016-06-15
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention overcomes the above-mentioned defects in the existing methods, considers the problem of affecting the germination rate of seeds or cannot completely kill germs, and proposes a bactericide and a sterilizing method for killing bacterial fruit spot bacteria in seeds

Method used

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  • Bacterial fruit blotch seed disinfectant and application thereof
  • Bacterial fruit blotch seed disinfectant and application thereof
  • Bacterial fruit blotch seed disinfectant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: The influence of different medicament treatments on germination rate and germination potential of infected seeds

[0033] (1) The treatment process of different agents:

[0034] a) The treatment process of 4% formaldehyde and 4% hydrochloric acid is as follows: soak the bacteria-carrying seeds CK2 in chemical reagents for 20 minutes, rinse repeatedly (rinsing for 3-5 minutes), and then soak in sterile water for 5 hours and 40 minutes.

[0035] b) The process of self-made reagents and existing pesticides is as follows: after soaking 6h in the fungicide (self-made medicament) of the present invention, existing pesticides and sterile water (blank control group) with bacteria-carrying seeds CK2, simply rinse (rinse 30 seconds-1 minute) and then dry uniformly;

[0036] c) The treatment process of sterile water (blank control group) is: after soaking healthy seeds CK1 and bacteria-carrying seeds CK2 in sterile water for 6 hours, rinse briefly (rinse for 30 seco...

Embodiment 2

[0047] Embodiment 2: the impact (dehydrogenase) of different medicament treatment on seed vigor

[0048] The determination method of seed vigor is mainly through the TTC method: the oxidized colorless triphenyl tetrazolium chloride (TTC), after dyeing, accepts the hydrogen generated by the dehydrogenase in the respiration process of vigorous seeds, and becomes reduced. The red triphenylformaldehyde (TTF). Therefore, the deeper the red color after dyeing, the higher the activity of seed dehydrogenase, and the stronger the seed vigor.

[0049] (1) Treatment of different agents:

[0050] Referring to the method of Example 1, the healthy seeds CK1 and the bacteria-carrying seeds CK2 were treated with different agents to obtain seeds treated with different agents. Wherein, the treatment process of 1% formaldehyde and 1% hydrochloric acid is the same as that of 4% formaldehyde and 4% hydrochloric acid.

[0051] (2) Seed dehydrogenase treatment process:

[0052] Take 30 seeds eac...

Embodiment 3

[0056] Embodiment 3: The influence of different chemical treatment on seed seedling rate and control effect

[0057] (1) Treatment of different agents:

[0058] Referring to the method of Example 1, the healthy seeds CK1 and the bacteria-carrying seeds CK2 were treated with different agents to obtain seeds treated with different agents.

[0059] (2) Control effect of greenhouse planting detection of seed disease incidence:

[0060] Each treatment gets 30 seeds, and every 4 seeds are planted on the four corners of the same small flowerpot, and each treatment plants 6 small pots. The seeds were normally cultured for 2 weeks after planting, and the incidence of cotyledons was investigated 2 weeks after emergence, repeated 3 times, and the control effect was calculated.

[0061] Implementation results:

[0062] Count the emergence rate and the number of diseased plants of seeds after different treatments, and calculate the control effect of the disease rate after the seeds are ...

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Abstract

The invention belongs to the field of disinfection and control of plant seed-borne diseases, particularly relates to disinfection, prevention and treatment of watermelon bacterial fruit blotch, and discloses a novel compound disinfectant.The disinfectant comprises chlorine dioxide (ClO2) mainly having a disinfection effect, sodium hypochlorite (NaClO) mainly having a germination promoting effect, and potassium iodate (KIO3) mainly having an effect of stabilizer By means of the disinfectant, detected watermelon seeds with bacterial fruit blotch bacteria and artificial simulating watermelon seeds with bacterial fruit blotch bacteria are processed, the germination rate and germination potential of seeds are promoted, and the disinfection effect is remarkable and is obviously better than that of a reported existing bacterial fruit blotch disinfectant and a reported existing disinfecting method.The disinfection method can easily, rapidly and thoroughly kill bacterial fruit blotch bacteria carried by watermelon seeds, is easy to operate, free of toxin and pollution and has huge actual application value.

Description

technical field [0001] The invention belongs to the field of sterilization and control of plant seed-borne diseases, in particular to the sterilization and control of watermelon and muskmelon infected by bacterial fruit spot. technical background [0002] Melon is an important economic crop in my country. According to the statistics of the Ministry of Agriculture of the People's Republic of China in 2012, the planting area of ​​melon in my country exceeds 2 million hectares 2 , the total output is 68.18 million tons, and the United Nations Food and Agriculture Organization database (FAOSTAT) statistical data in 2012 my country's watermelon planting area accounted for more than 59% of the world's total watermelon area, and the output accounted for 68.1%. As the world's largest country in the cultivation and production of watermelon, China has a very serious disease problem of watermelon. According to statistics from the Institute of Plant Protection, Chinese Academy of Agric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/12A01N59/00A01P1/00A01C1/00
CPCA01C1/00A01N59/00A01N59/12A01N2300/00
Inventor 李逸龙许玲朱品宽李云峰李郁琴
Owner EAST CHINA NORMAL UNIV
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