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Method for producing microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus

A microbial polysaccharide and Paenibacillus technology, which is applied in the field of producing microbial polysaccharide fermentation liquid by using Paenibacillus colloidus, achieves good application prospects, reduces the power consumption of aeration and stirring, and saves the effect of equipment requirements

Active Publication Date: 2013-03-06
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the new application fields of its polysaccharides and the optimization of the process to increase the yield of polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for producing microbial polysaccharide fermentation broth using Paenibacillus colloids is to convert Paenibacillus colloids ( Paenibacillus mucilaginosus ) ACCC10013 is inoculated in the medium containing carbon source and nitrogen source for fermentation, including the following process steps:

[0027] A, strain heat stimulation

[0028] Under sterile conditions, add the spores in the inclined surface of the test tube into sterile water, shake and wash the spores to make a spore suspension and place it in a sterile container, and place the sterile container with the spore suspension in boiling water. Heat evenly, take out the sterile container after 1 minute and cool it quickly;

[0029] B. Fermentation

[0030] B1. Preparation of culture medium

[0031] The culture medium is prepared and sterilized, cooled to 30°C, and the culture medium is composed of the following components by mass percentage:

[0032] Starch 5%, sucrose 5%, ammonium sulfate 0.5%, di...

Embodiment 2

[0041] After uniformly heating the sterile container for 10 minutes in step A in the present embodiment, the sterile container is taken out and rapidly cooled;

[0042] B. Fermentation

[0043] B1. Preparation of culture medium

[0044] The culture medium is prepared and sterilized, cooled to 30°C, and the culture medium is composed of the following components by mass percentage:

[0045] Starch 20%, sucrose 10%, ammonium sulfate 5%, dipotassium hydrogen phosphate 20%, magnesium sulfate 10%, ferric chloride 0.5%, calcium carbonate 5%, yeast paste 3%, the balance is sterile water, pH =7.5;

[0046] B2. Cultivation

[0047] Inoculate the treated strain in step A in the medium prepared in step B, the fermentation period is 60 hours, the culture temperature is 30°C, and the inoculum amount is strain: the mass percentage of the medium after sterilization is 8%; the air volume is adjusted For: 0-4 hours ventilation ratio is 1:.5VVM, 4-12 hours ventilation ratio is 1:0.7VVM, 12-2...

Embodiment 3

[0051] The step of the present embodiment is to place the sterile container containing the spore suspension in boiling water for uniform heating, and after 8 minutes, the sterile container is taken out and rapidly cooled;

[0052] B. Fermentation

[0053] B1. Preparation of culture medium

[0054] The culture medium is prepared and sterilized, cooled to 30°C, and the culture medium is composed of the following components by mass percentage:

[0055] Starch 15%, sucrose 8%, ammonium sulfate 3%, dipotassium hydrogen phosphate 15%, magnesium sulfate 8%, ferric chloride 0.3%, calcium carbonate 3%, yeast paste 1%, the balance is sterile water, pH =7.5;

[0056] B2. Cultivation

[0057] Inoculate the treated strain in step A in the medium prepared in step B, the fermentation period is 60 hours, the culture temperature is 30°C, and the inoculum amount is strain: the mass percentage of the medium after sterilization is 8%; the air volume is adjusted For: 0-4 hours ventilation ratio ...

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Abstract

The present invention belongs to microbial polysaccharide fermentation, and particularly relates to a method for producing a microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus, wherein Paenibacillus mucilaginosus is inoculated in a culture medium containing a carbon source and a nitrogen source to carry out fermentation, and bacteria heat stimulation, addition of a surfactant during a fermentation process, and other manners are performed to optimize the production process. With the present invention, the problem of low yield of polysaccharides in the fermentation broth in the prior art is solved, and advantages of high polysaccharide yield in the fermentation broth, good bacterial growth, short fermentation period and the like are provided.

Description

technical field [0001] The invention belongs to the fermentation of microbial polysaccharides, and particularly refers to a method for producing microbial polysaccharide fermentation liquid by using Paenibacillus colloids. Background technique [0002] Jelly-like Paenibacillus is Bacillus agrobacterium, and its colonies are round, colorless, raised, viscous, transparent or translucent, with neat edges. In the past few decades, it has been developed and utilized as the main functional strains of microbial fertilizers, and it has played an important role in the field of agricultural microbial preparations. In recent years, with the deepening of the research on Paenibacillus glioma, people have begun to pay attention to the research and application of its extracellular polysaccharides. However, the hot spots still focus on the analysis of polysaccharides produced by Bacillus glioma and the ability of polysaccharides to decompose silicate minerals. There are few studies on the...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01
Inventor 陈文杰章淑艳魏亚新李军秦艳梅赵从波韩涛王云鹏古述江刘丽娜
Owner 河北省微生物研究所有限公司
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