Method for producing microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus
A microbial polysaccharide and Paenibacillus technology, which is applied in the field of producing microbial polysaccharide fermentation liquid by using Paenibacillus colloidus, achieves good application prospects, reduces the power consumption of aeration and stirring, and saves the effect of equipment requirements
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Embodiment 1
[0026] The method for producing microbial polysaccharide fermentation broth using Paenibacillus colloids is to convert Paenibacillus colloids ( Paenibacillus mucilaginosus ) ACCC10013 is inoculated in the medium containing carbon source and nitrogen source for fermentation, including the following process steps:
[0027] A, strain heat stimulation
[0028] Under sterile conditions, add the spores in the inclined surface of the test tube into sterile water, shake and wash the spores to make a spore suspension and place it in a sterile container, and place the sterile container with the spore suspension in boiling water. Heat evenly, take out the sterile container after 1 minute and cool it quickly;
[0029] B. Fermentation
[0030] B1. Preparation of culture medium
[0031] The culture medium is prepared and sterilized, cooled to 30°C, and the culture medium is composed of the following components by mass percentage:
Embodiment 2
[0041] After uniformly heating the sterile container for 10 minutes in step A in the present embodiment, the sterile container is taken out and rapidly cooled;
[0042] B. Fermentation
[0043] B1. Preparation of culture medium
[0044] The culture medium is prepared and sterilized, cooled to 30°C, and the culture medium is composed of the following components by mass percentage:
[0045] Starch 20%, sucrose 10%, ammonium sulfate 5%, dipotassium hydrogen phosphate 20%, magnesium sulfate 10%, ferric chloride 0.5%, calcium carbonate 5%, yeast paste 3%, the balance is sterile water, pH =7.5;
[0046] B2. Cultivation
[0047] Inoculate the treated strain in step A in the medium prepared in step B, the fermentation period is 60 hours, the culture temperature is 30°C, and the inoculum amount is strain: the mass percentage of the medium after sterilization is 8%; the air volume is adjusted For: 0-4 hours ventilation ratio is 1:.5VVM, 4-12 hours ventilation ratio is 1:0.7VVM, 12-2...
Embodiment 3
[0051] The step of the present embodiment is to place the sterile container containing the spore suspension in boiling water for uniform heating, and after 8 minutes, the sterile container is taken out and rapidly cooled;
[0052] B. Fermentation
[0053] B1. Preparation of culture medium
[0054] The culture medium is prepared and sterilized, cooled to 30°C, and the culture medium is composed of the following components by mass percentage:
[0055] Starch 15%, sucrose 8%, ammonium sulfate 3%, dipotassium hydrogen phosphate 15%, magnesium sulfate 8%, ferric chloride 0.3%, calcium carbonate 3%, yeast paste 1%, the balance is sterile water, pH =7.5;
[0056] B2. Cultivation
[0057] Inoculate the treated strain in step A in the medium prepared in step B, the fermentation period is 60 hours, the culture temperature is 30°C, and the inoculum amount is strain: the mass percentage of the medium after sterilization is 8%; the air volume is adjusted For: 0-4 hours ventilation ratio ...
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