This invention relates to a method for promoting the
germination of buckwheat seeds under
cadmium stress and alleviating
cadmium toxicity during the
seedling stage. The method includes treating buckwheat seeds with a
melatonin reagent before
germination. The method involves contacting the
plant seeds with a
melatonin solution at a concentration of 100-500 μmol / L. The method provided by this invention can effectively improve the
germination potential and germination rate of buckwheat seeds under
cadmium stress, enabling uniform and rapid germination. This invention solves the important problem of how to ensure normal germination of buckwheat in cadmium-contaminated soil in agricultural production and has significant application value in production. This is likely because pretreatment with a
melatonin reagent can induce the expression of
antioxidant enzyme activity, scavenge the large number of free radicals generated by cadmium stress, alleviate
oxidative stress, reduce
oxidative damage, and maintain a low level of
membrane lipid peroxidation in the seeds, thereby promoting normal seed germination.