Watermelon seed disinfection technology for controlling occurrence of bacterial fruit blotch and application of watermelon seed disinfection technology
A watermelon and bacterial technology, applied in the field of watermelon seed disinfection to control bacterial fruit spot disease, can solve the problems of reduced seed germination rate, low vigor, and difficulty in seedling formation, and achieve germination speed and uniformity. Improve the germination rate and the effect of less reagent consumption
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Embodiment 1
[0035] The disinfection method of embodiment 1, seed and its impact on seed germination
[0036] 1. Disinfection method of seeds
[0037] (1) Disinfection method of seeds
[0038] Mix vermiculite and water at a weight ratio of 3:2, and then mix Guomi 1 (three batches), Guomi 2, Jingling, Jingxiu, Jingxin 3 and India Seedless 1 into the in the matrix. Under the condition of 20 ℃, the seeds of Guomi 1, Guomi 2 and Jingling were primed for 23 hours, the seeds of Indian seedless 1 were primed for 25 hours, and the seeds of Jingxiu and Jingxin were primed for 24 hours trigger processing. After the priming treatment is finished, sieve the substrate, quickly wash off the vermiculite on the seed surface, and after quickly wiping off the moisture on the seed surface with absorbent paper (if it is a large number of seeds tested, the seeds are packed in a nylon bag and shaken by a dryer for 2 minutes), spread the seeds on filter paper to dry at room temperature.
[0039] (2) Germina...
Embodiment 2
[0046] Embodiment 2, the impact of different concentrations of copper sulfate solution initiation treatment on seed germination
[0047] Vermiculite with different concentrations of CuSO 4 The solution is mixed evenly at a ratio of 3:2 by weight, and Jingxin 1 seed is evenly mixed into the matrix. Put it under the condition of 20°C, and initiate the treatment for 24 hours. After the priming treatment is finished, sieve the substrate, quickly wash off the vermiculite on the seed surface, and after quickly wiping off the moisture on the seed surface with absorbent paper (if it is a large number of seeds tested, the seeds are packed in a nylon bag and shaken by a dryer for 2 minutes), spread the seeds on filter paper to dry at room temperature, and the results are shown in Table 1.
[0048] As can be seen from Table 1, there are significant differences in the germination rates measured at different times between all treatments and control seeds (without any treatment-carrying s...
Embodiment 3
[0052] Embodiment 3, medicament triggers the impact on the seed health and the PCR detection of the BFB carrier situation of the seed
[0053] 1. The impact of chemical triggers on the health of seeds
[0054] Vermiculite with different concentrations of CuSO 4 The solution is mixed evenly at a weight ratio of 3:2, and then a portion of Jingxin 2 seeds confirmed to naturally carry BFB pathogens is evenly mixed into the matrix. Put it under the condition of 20°C, and initiate the treatment for 24 hours. After the priming treatment is finished, sieve the substrate, quickly wash off the vermiculite on the seed surface, and after quickly wiping off the moisture on the seed surface with absorbent paper (if it is a large number of seeds tested, the seeds are packed in a nylon bag and shaken by a dryer for 2 minutes), spread the seeds on filter paper to dry at room temperature, and use the seedling growth method to identify the health of the treated seeds, and the results are shown...
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