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Application of 1% isothiazolinone.2% methylene bisthiocyanate missible oil in prevention and treatment of bacterial fruit blotch, and 1% isothiazolinone.2% methylene bisthiocyanate missible oil

A technology of dithiocyanomethane and isothiazolinone, applied in application, biocide, disinfectant, etc., can solve problems such as infection and economic loss, and achieve the effects of promoting root growth, reducing pollution, and increasing germination rate

Active Publication Date: 2014-02-19
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to infecting watermelons and melons, the pathogen can also infect cucurbit crops such as pumpkins and cucumbers. Once it occurs, it will cause serious economic losses

Method used

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  • Application of 1% isothiazolinone.2% methylene bisthiocyanate missible oil in prevention and treatment of bacterial fruit blotch, and 1% isothiazolinone.2% methylene bisthiocyanate missible oil
  • Application of 1% isothiazolinone.2% methylene bisthiocyanate missible oil in prevention and treatment of bacterial fruit blotch, and 1% isothiazolinone.2% methylene bisthiocyanate missible oil
  • Application of 1% isothiazolinone.2% methylene bisthiocyanate missible oil in prevention and treatment of bacterial fruit blotch, and 1% isothiazolinone.2% methylene bisthiocyanate missible oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Indoor Toxicity Assay

[0031] Indoor toxicity was determined by the zone of inhibition method. A single colony of Pseudomonas spp. was inoculated in liquid KB medium, cultured at 28°C, 200rpm / min for 24h, OD 600 ≈0.3 (10 8 cfu / mL). When the temperature of the medium drops to about 50°C, add the bacterial solution into the medium (the ratio of the bacterial solution to the medium is 1:40) to make a plate. A 7mm diameter sterile filter paper disc was placed in the plate. Take 10uL of different concentrations of test agents and drop them into the filter paper. Sterile water was used as control, and each treatment was repeated 3 times. After culturing at constant temperature for 48 hours at 28°C, measure the diameter of the inhibition zone with a cross (see figure 1 ).

[0032] 2. Result Analysis

[0033] 2.1. Indoor virulence

[0034] The results of the toxicity test are shown in Table 1. It can be seen from Table 1 that among the 5 kinds of medicaments teste...

Embodiment 2

[0043] 1. Seed treatment

[0044] Adjust the Aac-5 bacterial solution to 10 8 cfu / mL, mixed at the ratio of 1g of melon seeds and 2mL of bacterial solution, soaked for 1h, and dried the seeds in an ultra-clean workbench. See Table 4 for the test agents, treatment concentrations and time. After the agent treatment, the seeds were rinsed with water for 3 times. The seeds were placed on the water agar plate, and the sterilized filter paper was soaked in water and covered the seeds. 50 seeds per dish, repeated 3 times. Accelerate the germination at 28°C, investigate the germination rate of the seeds after 16 hours, and measure the root length after 48 hours, so as to judge the effect of the chemical treatment on the germination of the seeds. The remaining seeds of the same treatment were sown in sterilized soil, and each treatment was repeated 3 times, with 15 seedlings per repetition. Clear water was used as a control. On the 5th day after emergence, the number of cotyledon ...

Embodiment 3

[0063] 1. Spray concentration detection

[0064] 72% agricultural streptomycin sulfate, 30% copper succinate and 1% isothiazolinone·2% dithiocyanomethane emulsifiable concentrate were used for the test agent, and 5 concentrations were set: 1200 times liquid, 1000 times liquid, 800 times liquid, 500 times liquid and 200 times liquid. Spray on melon seedlings and watermelon seedlings, repeat 4 times for each concentration, 15 seedlings for each repetition, control with clean water, and investigate the phytotoxicity after 7 days.

[0065] 2. Results and Analysis

[0066] 2.1 Analysis of test results

[0067] After spraying watermelon and melon seedlings with 72% agricultural streptomycin sulfate, copper succinate, and 1% isothiazolinone·2% dithiocyanomethane emulsifiable concentrate, the plants showed different drug resistance. Not the same. After spraying the leaves of watermelon and muskmelon with 3 kinds of agents at 1000 times of liquid, the plants all grew healthy and gr...

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Abstract

The invention provides an application of 1% isothiazolinone.2% methylene bisthiocyanate missible oil in the prevention and treatment of bacterial fruit blotch, and 1% isothiazolinone.2% methylene bisthiocyanate missible oil, and belongs to the biotechnical field. The optimum use concentration of the 1% isothiazolinone.2% methylene bisthiocyanate missible oil in the field spray prevention and treatment and the optimum soup concentration, the optimum processing time and the like used for processing infected seeds are obtained through the indoor toxicity test, seed processing and the field prevention and treatment in the invention, so the germination percentage of seeds can be improved, the growth of roots can be promoted, a technical support is provided for the scientific and safe use of the missible oil, and a theoretical basis is provided for the prevention and treatment of the fruit blotch.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of 1% isothiazolinone·2% dithiocyanomethane emulsifiable concentrate in preventing and treating bacterial fruit spot and the agent. Background technique [0002] Bacterial fruit blotch (BFB) of melons is a devastating bacterial disease on Cucurbitaceae crops, which occurs in many countries in the world and in many provinces of my country, and is a worldwide quarantine disease. The pathogen is Acidovorax citrulli Schaad et al., 2008. In 1998, Chinese scholar Zhang Rongyi and others discovered and reported the disease for the first time in Hainan Province, and described its symptoms in detail. In recent years, the disease has occurred in Xinjiang, Hainan, Henan, Fujian, Inner Mongolia, Shandong, Jilin, Heilongjiang and other provinces in China. [0003] The disease is a typical seed-borne disease. The pathogenic bacteria can survive on the seeds for 19 years and spread ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/48A01P1/00A01N43/80
Inventor 赵廷昌折冬梅董春玲杨玉文王铁霖孙柏欣
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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