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A watermelon seed disinfection technology and its application to control the occurrence of bacterial fruit spot

A watermelon and bacterial technology, applied in the field of watermelon seed disinfection to control bacterial fruit spot disease, can solve the problems of reduced seed germination rate, low vigor, and difficulty in seedling formation, and achieve the improvement of germination speed and uniformity, The effect of using less reagent and improving the germination rate

Inactive Publication Date: 2016-08-10
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Compared with common diploid watermelon seeds, triploid watermelon seeds have lower vitality and more difficult to grow seedlings
Fermentation treatment cannot be used in the seed harvesting process, which increases the possibility of seeds carrying pathogenic bacteria
On the other hand, conventional disinfection treatment methods are basically not applicable to triploid watermelon seeds, which is manifested in that the germination rate of seeds after treatment has decreased to varying degrees.
At present, there is no effective research on the disinfection technology for triploid watermelon seeds BFB, and related methods are urgently needed in production to improve the health of triploid watermelon seeds and control the occurrence of BFB

Method used

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  • A watermelon seed disinfection technology and its application to control the occurrence of bacterial fruit spot
  • A watermelon seed disinfection technology and its application to control the occurrence of bacterial fruit spot
  • A watermelon seed disinfection technology and its application to control the occurrence of bacterial fruit spot

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The disinfection method of embodiment 1, seed and its impact on seed germination

[0036] 1. Disinfection method of seeds

[0037] (1) Disinfection method of seeds

[0038] Mix vermiculite and water at a weight ratio of 3:2, and then mix Guomi 1 (three batches), Guomi 2, Jingling, Jingxiu, Jingxin 3 and India Seedless 1 into the in the matrix. Under the condition of 20 ℃, the seeds of Guomi 1, Guomi 2 and Jingling were primed for 23 hours, the seeds of Indian seedless 1 were primed for 25 hours, and the seeds of Jingxiu and Jingxin were primed for 24 hours trigger processing. After the priming treatment is finished, sieve the substrate, quickly wash off the vermiculite on the seed surface, and after quickly wiping off the moisture on the seed surface with absorbent paper (if it is a large number of seeds tested, the seeds are packed in a nylon bag and shaken by a dryer for 2 minutes), spread the seeds on filter paper to dry at room temperature.

[0039] (2) Germina...

Embodiment 2

[0046] Embodiment 2, the impact of different concentrations of copper sulfate solution initiation treatment on seed germination

[0047] Vermiculite with different concentrations of CuSO 4 The solution is mixed evenly at a ratio of 3:2 by weight, and Jingxin 1 seed is evenly mixed into the matrix. Put it under the condition of 20°C, and initiate the treatment for 24 hours. After the priming treatment is finished, sieve the substrate, quickly wash off the vermiculite on the seed surface, and after quickly wiping off the moisture on the seed surface with absorbent paper (if it is a large number of seeds tested, the seeds are packed in a nylon bag and shaken by a dryer for 2 minutes), spread the seeds on filter paper to dry at room temperature, and the results are shown in Table 1.

[0048] As can be seen from Table 1, there are significant differences in the germination rates measured at different times between all treatments and control seeds (without any treatment-carrying s...

Embodiment 3

[0052] Embodiment 3, medicament triggers the impact on the seed health and the PCR detection of the BFB carrier situation of the seed

[0053] 1. The impact of chemical triggers on the health of seeds

[0054] Vermiculite with different concentrations of CuSO 4 The solution is mixed evenly at a weight ratio of 3:2, and then a portion of Jingxin 2 seeds confirmed to naturally carry BFB pathogens is evenly mixed into the matrix. Put it under the condition of 20°C, and initiate the treatment for 24 hours. After the priming treatment is finished, sieve the substrate, quickly wash off the vermiculite on the seed surface, and after quickly wiping off the moisture on the seed surface with absorbent paper (if it is a large number of seeds tested, the seeds are packed in a nylon bag and shaken by a dryer for 2 minutes), spread the seeds on filter paper to dry at room temperature, and use the seedling growth method to identify the health of the treated seeds, and the results are shown...

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Abstract

The invention discloses a watermelon seed disinfection technology for controlling the occurrence of bacterial fruit spot disease. The technique involves using CuSO 4 The aqueous solution sterilizes the seeds. The results show that the treated seeds can effectively kill the bacterial fruit spot disease bacteria carried by the seeds, and reduce the occurrence of field fruit spot disease after planting. At the same time, the initiation treatment of the solid substrate can effectively improve the germination speed and uniformity of the diploid watermelon seeds, reduce the germination obstacles of the triploid watermelon seeds, and increase the final germination rate of the seeds.

Description

technical field [0001] The invention relates to the technical field of seed treatment, in particular to a watermelon seed disinfection technology for controlling bacterial fruit spot disease and its application. Background technique [0002] Bacterial fruit blotch (BFB) is the most important and destructive seed-borne disease in watermelon and melon production. The pathogen is Acidovorax citrulli (Ac), and it is a worldwide quarantine disease. It was first reported in the 1990s that the disease caused damage in my country. In recent years, large-scale serious damage has occurred in Xinjiang, Hainan, Inner Mongolia, Shandong and other places, causing serious economic losses. It is an urgent problem to be solved in the safe production of melon. Bacterial fruit spot is currently defined internationally as a seed-borne disease. Although the infection and pathogenic pathways of bacterial fruit spot have not been fully confirmed yet, it has been confirmed that seed contamination ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/08
Inventor 吴萍宋顺华许勇宫国义李丽丁海凤侯小亮
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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