Bio-control strain for preventing and controlling bacterial fruit blotch of watermelon

A biocontrol strain and bacterial technology, applied in the direction of bacteria, biocides, microorganisms, etc., can solve the problems of low control effect, pesticide residues, pathogenic bacteria resistance, etc., and achieve the effect of benefiting export trade and saving expenses

Inactive Publication Date: 2011-09-07
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] No better method for seed treatment has been found. The chemical agents used in the current production include agricultural streptomycin, which can be killed, new phytomycin, and bacteria-killed , Ye Shuangqing, Jiaruinong, succinic acid copper,...

Method used

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  • Bio-control strain for preventing and controlling bacterial fruit blotch of watermelon
  • Bio-control strain for preventing and controlling bacterial fruit blotch of watermelon
  • Bio-control strain for preventing and controlling bacterial fruit blotch of watermelon

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Embodiment Construction

[0025] A biocontrol strain JC65 for preventing and treating bacterial fruit spot disease of watermelon is Bacillus ( Bacillus

[0026] sp.), isolated from the rhizosphere soil of pear trees in the pear orchard of Deqing County, Zhejiang Province.

[0027] Biocontrol strains Bacillus sp. The method for JC65 to prepare biocontrol agent is:

[0028] 1) Inoculate the strain into LB culture medium and culture at 30°C with 180rpm shaking. After 12 to 16 hours, take samples in the ultra-clean workbench to measure the OD value at 600nm. During the measurement, use the uninoculated culture medium to adjust to zero. ;

[0029] 2) When the OD value is between 0.5 and 0.8, add the seed bacterial solution into a 200L fermenter with a fermentation medium at a volume ratio of 1:100, and vibrate at 250rpm at 30°C for 20 to 24 hours, and the pH of the fermentation solution will increase. The value is maintained at 7.0, the dissolved oxygen is 20%, and the ventilation volume is 5-7 m 3 / ...

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Abstract

The invention relates to a bio-control strain JC65 for preventing and controlling bacterial fruit blotch of watermelon, and belongs to the technical field of crop disease prevention and control. The bacteria are Bacillus sp.; and the bacteria are subjected to shaking culture for 20 to 24 hours in load balance (LB) culture solution at the temperature of 30 DEG C and at the revolution speed of 180rpm, then the solution is centrifuged for 10 minutes at the revolution speed of 6,000rpm, the wet bacteria and the bacteria preservation solution are prepared into bactericide in a ratio of 1:40 (g/ml), and the total concentration of living bacteria in the finished product is 1*10<9> to 1*10<12>cfu/ml. The bactericide is diluted to the concentration of 1*10<8>cfu/ml in use, the prevention effect on the bacterial fruit blotch of watermelon is 50 to 90 percent, and the yield increasing effect on a sick field is 10 to 200 percent. The growth promoting effect on the disease-free plants can reach 10to 200 percent, and the yield increasing effect on the disease-free field is 5 to 20 percent. The bactericide can be blended with water for spraying or made into compost for use, and can also be diluted for irrigating roots during transplanting.

Description

1. Technical field [0001] The invention relates to a bacillus biocontrol strain JC65 for preventing and treating watermelon bacterial fruit spot disease, which belongs to the technical field of crop disease prevention and treatment, and is specially used for pollution-free prevention and treatment of watermelon bacterial fruit spot disease. 2. Background technology [0002] Bacterial fruit blotch of watermelon (BFB) is a dangerous quarantine disease on watermelon, also known as bacterial spot, which was first discovered in Florida, USA by Crall and Schenck in 1969. It only described the symptoms of fruit spot disease, but did not identify the pathogenic bacteria; so far, fruit spot disease has been reported in many provinces and cities in my country, such as Xinjiang, Shaanxi, Hebei, Shanxi, Jilin, Fujian, Gansu and other places. The disease mainly harms the fruit, causing watermelon fruit to rot, etc., which can cause devastating losses. Once the disease occurs, the control ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/07
Inventor 郭坚华蒋春号陈云
Owner NANJING AGRICULTURAL UNIVERSITY
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