441 results about "Disease free" patented technology

The present invention provides methods, systems and equipment for the prognosis, diagnosis and selection of treatment of AML or other types of leukemia. Genes prognostic of clinical outcome of leukemia patients can be identified according to the present invention. Leukemia disease genes can also be identified according to the present invention. These genes are differentially expressed in PBMCs of AML patients relative to disease-free humans. These genes can be used for the diagnosis or monitoring the development, progression or treatment of AML.

Methods and diagnostic kits for determining whether a subject may develop a or for diagnosing a neurodegenerative disease. The method includes quantitating the amount of alpha-synuclein and total protein in a cerebrospinal fluid (CSF) sample obtained from the subject and calculating a ratio of alpha-synuclein to total protein content; comparing the ratio of alpha-synuclein to total protein content in the CSF sample with the alpha-synuclein to total protein content ratio in CSF samples obtained from healthy neurodegenerative disease-free subjects; and (c) determining from the comparison whether the subject has a likelihood to develop neurodegenerative disease or making a diagnosis of neurodegenerative disease in a subject. A difference in the ratio of alpha-synuclein to total protein content indicates that the subject has a likelihood to develop a neurodegenerative disease or has developed a neurodegenerative disease.

The invention provides a multiple propagation cutting seedling raising method for a stem tuber of paris polyphylla var. yunnanensis. The method is characterized by the following steps: cutting transversely a strong disease-free stem tuber of paris polyphylla var. yunnanensis into small segments according to stripe, cutting each segment into small blocks which have a stem scar, placing the cut blocks on a seedling bed spread with humus soil and sandy soil, covering the cut blocks with 3 to 5 centimeters of humus soil and sandy soil, keeping a 60 to 70 percent relative humidity of the soil, shading the cut blocks according to a 40 to 60 shading degree, temporary planting the cut blocks for one year and then transplanting seedlings of the paris polyphylla var. yunnanensis. With the method, the germination percentage of the stem tuber of the paris polyphylla var. yunnanensis reach 85 to 90 percent and the rooting rate of the stem tuber of the paris polyphylla var. yunnanensis is 40 to 50 percent. Propagation coefficient of the stem tuber of the paris polyphylla var. yunnanensis of the method is increased by more than 5 times than that of the prior rhizome segment seedling raising method of paris polyphylla var. yunnanensis.

The invention belongs to the field of agricultural fruit growing, and particularly provides a method for cultivating hybrid oranges. The method comprises the following steps that 1, disease-free seedlings are prepared; 2, soil cultivation is carried out, wherein a single plowshare of a high-horsepower tractor is adopted for deep ploughing and deep scarification, and the ploughing depth ranges from 65 cm to 70 cm; 3, the hybrid oranges are planted; 4, water and fertilizer management is carried out; 5, shaping and pruning are carried out; 6, flowers, leaves and fruits are managed; 7, plant diseases and insect pests are prevented and treated; 8, harvesting and the like are carried out in good time. The hybrid oranges can be cultivated efficiently, disease and pest damage can be controlled, the yield and quality of the fruits are improved, and the method is especially suitable for building hybrid orange orchards with the area of one hundred mu and above.

Birds carry many parasites and other diseases, which pose a health risk to humans which come into contact with their habitat. However, ultrasonic waves agitate and deter birds and other pests from roosting in nearby areas. The disclosed device utilizes solar energy to power a battery pack, which in turn activates both a tone generator and its adjoining amplifier to create and emit the annoying ultrasound. In this way, birds are dissuaded from gathering in particular areas where the ultrasonic device is in use, and humans are provided a more healthy and disease-free environment.

The invention discloses a tilapia fry rearing method. The tilapia fry rearing method comprises the following steps: breeding parent tilapia fries which are bigger than nine-sieve, picking parent tilapias which are disease free, injury free, strong and good in sexual maturity to rear, hybridizing the female parent tilapias and with the male parent tilapias according to ratio of stocking, picking up roes, incubating the roes with flowing incubating water in an incubating tank, cultivating the roes by marking in bold, and cultivating fries. Oviposition period is shortened by about seven days, incubating rate is as high as 67.9%, fry survival rate is 89.6%, each female tilapia lays 6700 fries each year averagely, fry production rate is improved by 30%, and the sizes of the fries are uniform. By means of the tilapia fry rearing method, fries can be reared all through a year, a large amount of tilapia fries are reared and the tilapia fries grow fast and are strong in stress resistance. The tilapia fries reared in mass are born at the same time, gonad developments are synchronous, sizes are the same, characters are unified, and heredity purity is high. Moreover, the tilapia fry rearing method has the advantages that incubating period is short, incubating efficiency is high, inheritable characters are good, fertility is strong, and fries can be reared in an overwintering mode.

The invention discloses a method for cultivating a thornless Chinese pricklyash tree, which comprises the following steps: (1) selecting a disease-free Baisha pepper between the beginning of June and the end of September as a stock, and selecting a small pepper bud as a scion; and (2) grafting the bud or branch of the tree grown up from the grafted bud last year on the disease-free Baisha pepper stock between the middle ten days of February and the last ten days of March in the coming year. The fruit of the Chinese pricklyash tree obtained by grafting by using the method has a strong flavor, and more importantly, the Chinese pricklyash tree is thornless so that the fruit of the Chinese pricklyash tree is easy to pick up and the cost of picking up the Chinese pricklyash tree is reduced.

The present invention relates to a commercially viable process for in vitro mass culture of Jatropha curcas. The process for in vitro mass culture of Jatropha curcas is simple, faster, and suitable for production of disease-free root tubers of uniform quality and employs media with a reduced concentration of phytohormones.

The invention discloses a longevity medicated wine and a preparation method thereof; according to traditional Chinese medicine theory, 34 natural Chinese herbal medicines such as polygonatum, ganoderma lucidum, ginseng, eclipta alba, radix polygoni multiflori, radix codonopsis, atractylodes rhizome, pine needle, black sesame seed, longan meat, red dates, wolfberry fruit, glossy privet fruit, radix polygalae, fructus psoraleae, asparagus, sweet scented osmanthus, epimedium, cistanche, white poria cocos, morinda root, eucommia bark, padix rehmanniae Preparata, fructus alpiniae oxyphyllae, the rhizome of chuanxiong, astragalus membranaceus, angelica, poria with hostwood, achyranthes root, safflower, radix paeoniae alba, rhizoma drynariae, bighead atractylodes rhizome and agilawood are put into 50 degree-above high quality rice liquor with the weight of 2-3 times of the total weight of the Chinese herbal medicines; and rock sugar, royal jelly and other auxiliary materials are added for preparation by a traditional process. The longevity medicated wine has the effects of activating blood, replenishing blood, dispersing blood stasis, nourishing heart and liver, tonifying spleen and stomach, moistening hair and strengthening muscles and bones, tonifying yuan yang and benefitting Yin deficiency, nourishing kidney, strengthening waist, tonifying Qi, relaxing body, resting mind, resisting senility, preventing aging, supporting the right and securing the root, enhancing immunity and the like. A variety of material components produced by combination of the medicines can play the ideal effect of treating disease when the people is in disease and prolonging life when the people is disease-free.

The invention discloses a preparing method of mulberry and honeysuckle fruit wine. The preparing method comprises the following steps of: selecting ripe, fresh and disease-free mulberry, washing, pulping, preparing by using complex phosphoesterasum, juicing, and filtering; and washing honeysuckle, drying, smashing, boiling, mixing the boiled honeysuckle with the obtained mulberry juice, fermenting by using yeast, ageing, packaging, and sterilizing, so as to obtain a finished product. For the processing method, an enzymolysis technology in which the complex phosphoesterasum is adopted is adopted, the decomposition rate of cellulose and hemicellulose of the mulberry is effectively improved, the juice yield of the mulberry is improved, and nutrient substances of the mulberry are well reserved, the honeysuckle is subjected to steps of drying, smashing and boiling, so that nutritional ingredients of the honeysuckle can be effectively separated out, the utilization ratio of the raw materials is improved, after the honeysuckle is mixed with the mulberry juice, the nutrition of the fruit wine is improved, and the fruit wine has mellow mouthfeel, a rich fruity flavor, and stable color, and also has the functions of delaying aging and enhancing immune.

The invention discloses a processing method of a drink of snow pears braised with loquat leaves and crystal sugar, which comprises the following steps in order: 1) selecting fresh, ripe, and disease-free loquat leaves, disease-free and damage-free snow pears, and crystal sugar as raw materials; 2) pretreating the raw materials; 3) putting sheet loquat leaves, block snow pears, and crystal sugar in a gauze bag, braising the raw materials with water; 4) adding vitamin C and citric acid into the filtered braised solution; 5) performing hot filling of the blended braised solution at 80-100 DEG C;6) performing high temperature sterilization treatment of the filled braised solution to obtain the drink of snow pear braised with loquat leaf and crystal sugar. The drink can not only bring into full play of the edible and medicinal value of loquat leaves, but also deeply explore the potential economic benefits of loquat leaves and snow pears, and reveals Chinese traditional characteristics.

A method for pickling white ginger relates to the technical field of pickled products. The method comprises the steps as follows: fresh, disease-free and insect pest-free white ginger produced in Tongling City, China is selected as a main raw material in September to October every year, and is separated, peeled and rinsed; the rinsed white ginger is immersed in mountain spring water for 6-7 hours, as a result, the surface of the peeled white ginger becomes white; the immersed white ginger is cut into sheets or strips for pickling, salt is added to the white ginger according to a proportion of 6:100, during pickling, the slat is sprinkled on each layer of white ginger pickled in a tank or a basin, the tank or the basin is well covered after the salt and the white ginger are placed well, pickling lasts for 7-8 hours, and the white ginger is turned over at the intermediate of the pickling period; the pickled white ginger is dragged out and rinsed for 1-2 hours, and partial salt is removed; the rinsed white ginger is dried under the sun, old white ginger is dried for the half of a day while tender white ginger is dried for one day, and partial moisture is evaporated; the dried white ginger is immersed in a flavoring liquid for 5-7 days, as a result, the white ginger can be eaten. The white ginger pickled through adopting the method has a sweet and sour taste, is provided with an aromatic and crisp mouth feeling, does not contain chemical additives, can be stored for 12 months at the normal temperature, and is simple in preparation method and convenient to operate.

The invention discloses a selecting and breeding method of a polyploidy novel mulberry tree breed. The breeding method comprises the following steps: implementing artificial hybridization and chromosome induction of polyploidy and other modes by selecting mulberry tree diploid hybridized mulberry seeds or hybridized seed mulberry sapling disease-free plants as materials; selecting out bud variation and branches the shape of which are similar to the polyploidy or plants by excellent plant, single plant selection and system selection; carrying out simple bud grafting on branch break bud variation the shape of which is similar to the polyploidy or plants in a primary selection garden; carrying out microscopical examination on the selected polyploidy single plants; establishing a system selection garden after determining that the single plants are polyploidy; and breeding a mulberry tree polyploidy novel breed by establishing product test gardens and parent gardens. The novel breed has extremely-high quality, ultrahigh yield and wide applicability; and in the actual production, a mulberry tree breed with ultrahigh yield matched with early growth, middle growth and late growth mulberry can be developed and bred, adapts to the requirement of pluralized and multiple silkworm breeding production all the year around on mulberry breeds and has obviously-improved mulberry yield, mulberry quality and other economic characters.

The invention relates to a bio-control strain JC65 for preventing and controlling bacterial fruit blotch of watermelon, and belongs to the technical field of crop disease prevention and control. The bacteria are Bacillus sp.; and the bacteria are subjected to shaking culture for 20 to 24 hours in load balance (LB) culture solution at the temperature of 30 DEG C and at the revolution speed of 180rpm, then the solution is centrifuged for 10 minutes at the revolution speed of 6,000rpm, the wet bacteria and the bacteria preservation solution are prepared into bactericide in a ratio of 1:40 (g/ml), and the total concentration of living bacteria in the finished product is 1*10<9> to 1*10<12>cfu/ml. The bactericide is diluted to the concentration of 1*10<8>cfu/ml in use, the prevention effect on the bacterial fruit blotch of watermelon is 50 to 90 percent, and the yield increasing effect on a sick field is 10 to 200 percent. The growth promoting effect on the disease-free plants can reach 10to 200 percent, and the yield increasing effect on the disease-free field is 5 to 20 percent. The bactericide can be blended with water for spraying or made into compost for use, and can also be diluted for irrigating roots during transplanting.

The invention discloses a method for breeding of cabbage seedlings. An artificially prepared substrate is adopted to breed cabbage seedlings in a rainproof greenhouse, harm of diseases, pests and weeds which can be spread in soil can be avoided, various effects on the cabbage seedlings caused by difficult-to-control defects of physical and chemical properties of soil can be eliminated completely, normal growth of the cabbage seedlings can be ensured, environmental conditions of the nutrients, temperature, moisture and light required for growth of the cabbage seedlings can better provided, adverse effects of dramatic changes in the environmental conditions on the seedling breeding process can be avoided, smooth breeding of the cabbage seedlings can be ensured, and commercialized and intensified scale production of the cabbage seedlings can be realized. Large quantities of uniform, delicate, disease-free and robust cabbage seedlings can be cultivated in a short term, the breeding period of the seedlings can be shortened, the uniformity of transplanted seedlings can be improved, concentrated transplanting of the cabbage seedlings in a contiguous region in the best seasons can be ensured, the consistency and uniformity of growth of cabbage plants can be improved, and a good foundation can be laid for balanced and efficient production of cabbages.

Disclosed is a Chinese sucker artificial breeding method. A breeding pool is a circular or square cement pool, the area of the breeding pool is in a range of 12m<2> to 20m<2>, and the breeding pool is 0.7m to 1.0m. The water circulation and drainage function is required to be good, the water quality is required to be transparent and clear, harmful heavy metal ions are required to be absent, primary feeding water of the pool is between 70cm and 100cm, water is sterilized through 0.3mg/L dibromohydantoin, and strong and disease-free individuals are selected to be bred in the breeding pool. By using the method for scientific and fine breeding, the yield effect within a short period is apparent, the living rate is high, and the method is suitable for industrialized large-scale development.

The invention discloses a tissue culture method of strawberries and belongs to the technical field of agricultural biological tissue culture. The tissue culture method comprises the following steps: 1, selecting explants, that is, taking the creeping stem tips of healthy and strong disease-free strawberries, with the sizes of 0.3-0.4 mm as explant stem tips for tissue culture in the afternoon on a sunny day at the fifth to sixth months; 2, preparing culture mediums including (1) a differential medium, (2) a subculture solid medium and (3) a root induction solid medium; 3, sterilizing; 4, inoculating; 5, transplanting tissue culture seedlings. According to the tissue culture method, mercury chloride is selected as a sterilizing agent, so that the sterilizing performance is relatively good and the time is easy to control; large and deep green rooting seedlings each with more than three leaves and a large amount of thick and strong adventitious roots directly growing from stem base are selected, so that the survival rate is relatively high in general; the method is relatively simple in overall performing process and relatively easy to perform, can be used for quickly propagating a large amount of virus-free strawberry seedlings, and is very high in practicality.

The invention discloses a high-efficiency tobacco seedling cultivation method. The method includes the steps of accurately sowing seeds, applying fertilizer by stages, drawing in a sunshade net during the large-cross period of tobacco to receive sunlight illumination, opening a side film of a greenhouse during a seedling hardening period, spraying rare earth nutrient solution during the large-cross period to ensure nutrient required by tobacco seedlings, keeping timely ventilation to replenish the greenhouse with carbon dioxide so as to meet the requirement of photosynthesis when it is sunny, cutting leaves when the leaves of the tobacco seedlings overlap and are shaded, monitoring sanitary conditions constantly, cleaning and disinfecting the greenhouse and spraying insecticide every half a month, filling a floating disk with basic media, sowing the seeds in holes of the floating disk filled with the basic media, allowing the floating disk to float in a nutrition pool of the greenhouse, applying the fertilizer into water in the nutrition pool, carrying out management on the seedling cultivation process, hardening the seedlings before transplantation and finally directly transplanting the seedlings to a field after the seedlings are hardened. The high-efficiency tobacco seedling cultivation method has the advantages of improving the emergence uniformity of the seedlings, enhancing the root vitality of the tobacco seedlings, promoting the growth and development of the tobacco seedlings, improving the quality of the tobacco seedlings, being beneficial for cultivating the disease-free and strong tobacco seedlings and low in production cost, and improving the strong seedling rate.

The invention provides a domestication cultivation medium for bletilla striata tissue culture seedlings. The domestication cultivation medium is compounded by mixing disease-free loam, humus soil and perlite according to certain volume percentages and stirring. The medium of the invention is reasonable in formula material selection and ratio, comprises the simple and efficient components, gives full play to water retaining, ventilation and nutrition supplement which are needed by domestication cultivation, is wide in employed material resource and substantially reduced in cost, and possesses extremely strong popularization value. The bletilla striata tissue culture seedlings bred by employing the domestication cultivation medium of the invention are good in growth vigor, are strengthened and aligned, and the finally obtained bletilla striata plants are flourished in rhizome and strong. The domestication time by employing the domestication cultivation medium is relatively short, and only after 2-4 months, the seedlings can be transplanted into a field for cultivation with a survival rate of 85% or more, and the production efficiency is substantially improved.

The invention discloses a filbert shoot cutting seedling raising method. The filbert shoot cutting seedling raising method comprises, from middle June to early July, choosing robust, pest and disease-free plants as objects to cut, collecting one-year-old branches robust in growth and high in degree of lignification from the base of a tree, clipping out tender parts of the middle and upper portionsof the branches, performing scion cutting and immersion treatment and then planting the clipped parts in a nursery bed with a temperature of 25-28 DEG C, an air humidity higher than 90% and a substrate moisture content higher than 50%-60% for seedling raising. According to the filbert shoot cutting seedling raising method, tannin molecules can enter into amorphous regions of silk fibroin fiber and settle inside the fiber by being bonded with the silk fibroin fiber via hydrogen bonds, Van der Waals' force and the like, and are in multi-point bond in forms of hydrogen bonds with peptidyl-OH, -NH-CO-, -NH2 and-COOH of proteins of sericin to immobilize the sericin; immersion in acetic acid aqueous solution can extrude a large amount of tannin inside scion cells and absorb the tannin onto silks to reduce the content of tannin inside scions as well as the browning amount of tannin, to avoid browning and rotting of cuts of the scions due to oxidation of tannin, to promote rooting of the scions and further to improve the cutting survival rate.

The invention relates to a method for promoting grafting survival by reserving rootstock branches for shading. The method comprises the following steps: (1) selecting a stock plant: selecting a healthy and disease-free rootstock stock plant with a diameter at a breast height of 3-20 cm; (2) processing the stock plant: reserving 3-5 branches in southeast, south and southwest directions below a grafting part to be used as shading branches; (3) performing grafting: according to a bark inserting grafting method, grafting a scion, and paying attention to not damage the reserved branches of the rootstock; and (4) performing management after grafting: after the scion germinates, for a rootstock trunk which has no side branches or reserved shading branches when the stock plant is processed, selecting newly-germinated branches in southeast, south and southwest directions at the lower part of the grafted part in time to form shading branches, and removing excess rootstock branches to form favorable shading protection for the scion. The method provided by the invention provides appropriate shading conditions for grafted newly-germinated sprouts of the scion through scientifically reserving the rootstock branches, thereby improving a survival rate and preservation rate of seedling grafting.

The invention provides a tissue culturing rapid propagation method for radix scrophulariae and belongs to the technical field of agricultural propagation. The method mainly comprises the following technical steps for operation and treatment: (1) while harvesting the radix scrophulariae, selecting the disease-free, robust, white and 3-4cm long embryos as explants, and performing basic culturing on the embryos of the radix scrophulariae; (2) taking the embryos of the radix scrophulariae after the basic culturing, performing virus-free culturing, performing heat treatment for 30 days on the acquired tissue cultured seedlings at 40 DEG C, and then picking off 0.2cm stem tips, and taking the acquired stem tip test tube plantlets as the virus-free seedlings of the radix scrophulariae; (3) performing multiplying tissue culturing rapid propagation cultivation on the virus-free seedlings of the radix scrophulariae; (4) performing rooting culturing by performing rooting propagation on the virus-free seedlings of the radix scrophulariae after the cultivation; and (5) moving the rooted seedlings of the radix scrophulariae to the environment of strong light illumination, hardening the seedlings for 3 days, and transplanting the seedlings after the hardening to a matrix containing soil, pleurotus eryngii residues and vermiculite at the ratio of 1:1:1, and transplanting the seedlings to a farm field after the seedlings grow stably. The tissue culturing rapid propagation method for radix scrophulariae provided by the invention is fit for industrial seedling breeding; according to the method, the survival rate of the seedlings is greatly increased and the happening of diseases is effectively prevented; and the method is economical and practical.

The present invention relates to a commercially viable process for in vitro mass culture of Jatropha curcas. The process for in vitro mass culture of Jatropha curcas is simple, faster, and suitable for production of disease-free root tubers of uniform quality and employs media with a reduced concentration of phytohormones.