Tissue culturing rapid propagation method for radix scrophulariae

A technology of tissue culture rapid propagation and Scrophulariaceae, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of yield and quality decline, virus accumulation, lack of varieties, purification and rejuvenation, etc., to prevent the occurrence of diseases, Improve the survival rate, the method is economical and practical

Inactive Publication Date: 2015-12-30
耿跃
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Problems solved by technology

[0003] The reproduction method of wild Scrophulariaceae is to use seeds for sexual reproduction, while Yaonong adopts asexual reproduction method for cultivating Scr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for tissue culture and rapid propagation of Scrophulariaceae, characterized in that it mainly includes the following operating and processing steps:

[0022] (1) Basic training:

[0023] When harvesting Scrophulariaceae, select disease-free, robust, white, 3-4cm long seed buds as explants, soak them in 70% alcohol solution for 8 seconds under aseptic conditions, and then use 0.1% mercuric solution Soak for 10 minutes, soak in sterile water for 6 to 7 times, and carry out basic culture of the seed buds of Scrophularia scrophulariae. The formula components of the basic medium are: MS+6-BA0.6mg / L, NAA0.12mg / L, maltose 12g / L, agar 3g / L;

[0024] (2), detoxification culture:

[0025] Take the Scrophulariaceae sub-buds after the above-mentioned basic culture, carry out virus-free culture, and after the obtained tissue-cultured seedlings are heat-treated at 40° C. for 30 days, the shoot tips of 0.2 cm are stripped, and the obtained shoot-tip test-tube plantlets are...

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Abstract

The invention provides a tissue culturing rapid propagation method for radix scrophulariae and belongs to the technical field of agricultural propagation. The method mainly comprises the following technical steps for operation and treatment: (1) while harvesting the radix scrophulariae, selecting the disease-free, robust, white and 3-4cm long embryos as explants, and performing basic culturing on the embryos of the radix scrophulariae; (2) taking the embryos of the radix scrophulariae after the basic culturing, performing virus-free culturing, performing heat treatment for 30 days on the acquired tissue cultured seedlings at 40 DEG C, and then picking off 0.2cm stem tips, and taking the acquired stem tip test tube plantlets as the virus-free seedlings of the radix scrophulariae; (3) performing multiplying tissue culturing rapid propagation cultivation on the virus-free seedlings of the radix scrophulariae; (4) performing rooting culturing by performing rooting propagation on the virus-free seedlings of the radix scrophulariae after the cultivation; and (5) moving the rooted seedlings of the radix scrophulariae to the environment of strong light illumination, hardening the seedlings for 3 days, and transplanting the seedlings after the hardening to a matrix containing soil, pleurotus eryngii residues and vermiculite at the ratio of 1:1:1, and transplanting the seedlings to a farm field after the seedlings grow stably. The tissue culturing rapid propagation method for radix scrophulariae provided by the invention is fit for industrial seedling breeding; according to the method, the survival rate of the seedlings is greatly increased and the happening of diseases is effectively prevented; and the method is economical and practical.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to a method for tissue culture and rapid propagation of Scrophulariaceae. Background technique [0002] Scrophulariaceae is a herbaceous plant of the family Scrophulariaceae, which was first recorded in "Shen Nong's Materia Medica". It has the effects of cooling blood and nourishing yin, purging fire and detoxifying. Redness, sore throat, diphtheria, carbuncle and sores are endemic species in my country. At present, they are only naturally distributed in China. They are cultivated in most parts of the country, mainly produced in Zhejiang, Anhui, Hubei, Guizhou and other places. It is a commonly used bulk medicinal material. As a raw material of Chinese patent medicine, it can produce dozens of Chinese patent medicines such as Tianwang Buxin Pill, Tianma Pill, Mailuoning, Qingyanhou Mixture, etc. Now it is also used in the production of cosmetics as a plant lubricant, an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 耿跃
Owner 耿跃
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