Tissue culture method of strawberries

A tissue culture and strawberry technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high-quality strawberry virus-free seedlings production and breeding difficulties, susceptibility to various viruses, strawberry production hazards, etc., to achieve implementation Easy, easy time, high survival rate effect

Inactive Publication Date: 2014-12-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

Since strawberries are mostly grown asexually, they are prone to infection with various viruses
Mainly Strawberry Mottle Virus, Strawberry Mild Yellow Edge Virus, Strawberry Shrinkage Virus and Strawberry Vein Virus, Strawberry Virus Diseases cause serious harm to strawberry production, reduc

Method used

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with specific embodiments.

[0026] A strawberry tissue culture method is completed by the following steps: Step 1, explant selection; Step 2, culture medium preparation; Step 3, sterilization; Step 4, inoculation; Step 5, group seedling transplanting.

[0027] In step 1, in the sunny afternoon in May to June, take the stolon tips of healthy and disease-free strawberry plants, which are 0.3 to 0.4 mm in size, as explants for tissue culture.

[0028] In step 2, the stem tips taken in the above step 1 are treated with different sterilizing agents and washed with sterile water, and then transferred to differentiation medium (the basic medium used for culturing strawberry stem tips to induce bud differentiation is MS, additional 6-BA0.5mg / L, NAA0.2mg / L, sucrose 30g / L, agar powder 6.5g / L, pH value adjusted to 5.8~6.0); culture conditions are 25±2℃, 12 hours of light per day, light Strength 2000Lx is approp...

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Abstract

The invention discloses a tissue culture method of strawberries and belongs to the technical field of agricultural biological tissue culture. The tissue culture method comprises the following steps: 1, selecting explants, that is, taking the creeping stem tips of healthy and strong disease-free strawberries, with the sizes of 0.3-0.4 mm as explant stem tips for tissue culture in the afternoon on a sunny day at the fifth to sixth months; 2, preparing culture mediums including (1) a differential medium, (2) a subculture solid medium and (3) a root induction solid medium; 3, sterilizing; 4, inoculating; 5, transplanting tissue culture seedlings. According to the tissue culture method, mercury chloride is selected as a sterilizing agent, so that the sterilizing performance is relatively good and the time is easy to control; large and deep green rooting seedlings each with more than three leaves and a large amount of thick and strong adventitious roots directly growing from stem base are selected, so that the survival rate is relatively high in general; the method is relatively simple in overall performing process and relatively easy to perform, can be used for quickly propagating a large amount of virus-free strawberry seedlings, and is very high in practicality.

Description

field of invention [0001] The invention belongs to the technical field of agricultural biological tissue culture, and in particular relates to a strawberry tissue culture method. Background technique [0002] As a seasonal fresh fruit, strawberry has been widely planted in my country due to its outstanding economic value and ecological function. Since strawberries are mostly asexually propagated, they are susceptible to various viruses. Mainly strawberry mottle virus, strawberry mild yellow edge virus, strawberry shrunken virus and strawberry vein virus, strawberry virus diseases cause serious harm to strawberry production, reduce strawberry production and seriously affect production. [0003] Due to the needs of production and life and the limitations of existing cultivation conditions, it is difficult to produce and breed high-quality strawberry virus-free seedlings with high yield, good quality and strong resistance. Contents of the invention [0004] Purpose of the...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 胡德龙颜志明王全智谭晓燕陆建兰蔡善亚
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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