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Composite fungus agent for preventing rot knot nematode disease of vegetable in greenhouse

A root-knot nematode and compound bacterial agent technology, which is applied in biocides, animal repellents, plant growth regulators, etc., can solve the problems of large-scale use of Bacillus puncture and high cost of use, and achieve cost savings , the effect of reducing dosage

Active Publication Date: 2005-01-26
南京农大生物源农药创制有限公司
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Problems solved by technology

[0004] Technical Problems The purpose of this invention is to provide and develop a method for preventing and controlling vegetables in view of the defects in the prior art that the biocontrol agent Bacillus puncture, which has a relatively high control effect on root-knot nematode disease, cannot be used on a large scale and has high cost of use. Compound bacterial agent for root-knot nematode disease, the control effect of the agent can reach more than 25%, and it has the function of increasing production

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Embodiment Construction

[0016] The compound bacterial agent provided by the invention is composed of Bacillus circulans strain B130 (Bacillus circulans,) and Bacillus subtilis strain J1 (Bacillus subtilis), which are known to be non-pathogenic to humans, animals and plants.

[0017] For the strain B130, please refer to the literature: Guo Jianhua et al. 1994. Preliminary study on the properties of antagonists against R. solanacearum biocontrol bacteria. Journal of Nanjing Agricultural University. 18(2): 59∽62. For strain J1, see the literature: Guo Jianhua et al. 2001. Screening of antagonistic strains against pepper bacterial wilt and determination of field control efficacy. China Biological Control. 17(3): 101 ∽106.

[0018] Each of the above bacterial strains belongs to known and commonly used bacterial strains, which are preserved in the plant pathology laboratory of Nanjing Agricultural University (the laboratory where the inventor is located), which can guarantee long-term supply to the society...

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Abstract

A biological control bacteria formulation for preventing and curing green house vegetable root knot nematode disease is disclosed, specially being used for prevention and cure root knot nematode of cucumber, tomato, pepper etc, which is composed of Bacillus circulans B130 and Bacillus subtilis J1, The preparation method is: mixing the above-mentioned bacteria according to 2:1, 200rpm shaking 24-36 hours in PDA culture liquid in 33 DEG C, 6000rpm centrifuging, preparing formulation using mixed bacteria and bacteria preservative fluid according to 1:40(g / ml), the alive bacteria concentration being 1x10<9>-1x10<12>cfu / ml. The bacteria formulation can be used in dunghill, root irrigation while transplanting after dilution. The preventing effect of bacteria formulation to vegetable root knot nematode disease can reach 30-40%, yield promoting effect is more than 85-96%.

Description

1. Technical field [0001] The compound bacterial agent (PGPR bacterial agent) formula for preventing and treating root-knot nematode disease of greenhouse vegetables belongs to the technical field of crop disease prevention and treatment, and is specially used for pollution-free prevention and control of root-knot nematode disease of vegetables. 2. Technical background [0002] At present, the planting area of ​​vegetables in protected areas in my country continues to expand. Because the temperature and humidity conditions in protected areas are suitable for the growth and reproduction of root-knot nematodes, coupled with the continuous cropping of the same host crop or continuous planting of host crops, the disease of root-knot nematodes has increased year by year. At present, chemical pesticides are mainly used for the prevention and control of vegetable root-knot nematode diseases, such as: chloroform, dichlorvos, methylisothiazolin, abamectin, etc. However, the use of ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02
Inventor 郭坚华丁国春
Owner 南京农大生物源农药创制有限公司
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