Immune colloidal gold test strips for detection of fruit spot fungus and its monoclonal antibody and hybridoma cell line

A technology for detecting fruit spot disease and test paper, which is applied in the field of bioengineering, can solve the problem of low sensitivity of melon bacterial fruit spot disease, achieve remarkable technical progress, and achieve high detection specificity and sensitivity
CN104991069BActive Publication Date: 2016-06-29UNIV OF SHANGHAI FOR SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
UNIV OF SHANGHAI FOR SCI & TECH
Publication Date
2016-06-29

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Abstract

The invention relates to a melon bacterial fruit blotch immune colloidal gold rapid detection test strip. The test strip comprises a sample pad, a colloidal gold sample closely connected with the sample pad, a cellulose membrane arranged on the colloidal gold pad and closely connected with the colloidal gold pad, and a water absorption pad closely connected with the other end of the cellulose membrane, one end of the cellulose membrane away from the colloidal gold pad is provided with a quality control line, and the cellulose membrane between the quality control line and the colloidal gold pad is provided with a detection line, wherein the colloidal gold pad is provided with a monoclonal antibody coupling with colloidal gold, the monoclonal antibody is secreted by a hybridoma cell strain with the preservation number of CGMCC NO.10413, and the detection line is formed by the monoclonal antibody. The fruit blotch immune colloidal gold rapid detection test strip adopts the colloidal gold pad and the detection lime to be respectively sprayed with the new screened monoclonal antibody and has high detection specificity and sensitivity on fruit blotch. The invention also relates to monoclonal antibody for the test strip, and the hybridoma cell strain thereof.
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Description

technical field

[0001] The invention belongs to the field of bioengineering, and in particular relates to a melon bacterial fruit spot fungus, specifically a colloidal gold rapid detection test strip for the melon bacterial fruit spot fungus and its monoclonal antibody and hybridoma cell strain. Background technique

[0002] Melon fruit spot disease is a serious bacterial disease that received widespread attention in the late 1980s when a destructive outbreak occurred on watermelons in several states in the United States. spread within the range. The pathogen is Gram-negative bacteria Acidovorax avenae subsp.citrulli (Aacidovorax avenae subsp.citrulli, Aac), which is a highly destructive species-borne pathogen. So far, there are no commercial cultivars that are completely resistant to the pathogen.

[0003] Melon fruit spot bacteria, also known as fruit spot bacteria, can cause diseases in Cucurbitaceae plants such as watermelons, melons, and pumpkins. According to reports...

Claims

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