144 results about "Lateral flow test" patented technology

The invention relates to an extracellular vesicle surface protein detection paper-based kit and application thereof, and belongs to the technical field of biology. The technical problems that an existing sEVs detection technology is insufficient in specificity, depends on professional instruments and cannot meet the instant detection requirement are solved. According to the method, sEVs are synergistically captured through double aptamers, the target specificity is improved, signals are amplified in a cascaded mode through hyper-branched rolling circle amplification (HRCA) and a CRISPR / Cas12a system, and visual detection is achieved by detecting FAM-biotin probe separation before and after cutting through a transverse flow test strip. According to the method, interference of free protein is effectively avoided through double-target recognition, and the detection limit is as low as 406 parts / mL; cRISPR and a paper-based technology are combined to realize high sensitivity and instant detection; the system is simple and convenient to operate, does not need professional instruments, is suitable for tumor diagnosis and treatment monitoring of clinical samples, and provides a portable solution for precise medical treatment.

The invention relates to a nanofiber coated lateral flow test system for the rapid, accurate and more sensitive determination of HbA1c levels in patients with Type 2 diabetes.

A biofluid-based diagnostic screening system and associated methods are disclosed for screening a volume of biofluid of a patient for an at least one medical condition. In at least one embodiment, the system provides a lateral flow test strip, a test housing sized and configured for removably receiving the test strip therewithin, and a user application residing locally in memory on an imaging device and configured for screening a volume of biofluid of a patient, as deposited on the test strip, for an at least one medical condition.

The invention relates to the technical field of gender identification of Chinese softshell turtles, in particular to primers, a kit and a method for identifying gender of Chinese softshell turtles and application. According to the primer, the kit, the method and the application, on the basis of a target sequence as shown in SEQ ID NO: 3, genome DNA of the Chinese softshell turtles can be accurately amplified, and the gender of the Chinese softshell turtles can be accurately identified according to an amplification product. Furthermore, the PCR amplification product is subjected to a lateral flow test strip chromatography reaction, so that the gender of the Chinese softshell turtles can be visually identified, the identification universality is high, the identification range is wide, and the method has very wide application value.

The invention relates to an RPA (recombinase polymerase amplification) detection primer and a probe for rapidly detecting pseudomonas aeruginosa in drinking water. The primer and the probe show good inclusiveness and exclusiveness. Meanwhile, the invention also provides a rapid detection method of pseudomonas aeruginosa based on the detection primer and the probe, the method can adopt a lateral flow test strip for rapid detection, and a sensitivity test result developed by taking a standard strain lysis solution as a template shows that LOD50 of the rapid screening detection method is 2.4 * 10 < 3 > CFU / mL.

The invention discloses a method and a kit for visually detecting phytophthora parasitica var nicotianae of brassicaceous vegetables, and particularly relates to a method and a kit for visually detecting phytophthora parasitica var nicotianae based on an RPA-CRISPR / Cas12a system. A specific RPA primer pair and crRNA for detecting the brassicaceous vegetable phytophthora parasitica var nicotianae are designed according to the difference of ITS sequences of the brassicaceous vegetable phytophthora parasitica var nicotianae and the sibling species of the brassicaceous vegetable phytophthora parasitica var nicotianae. The brassicaceous vegetable phytophthora parasitica var nicotianae is detected through double specificity of RPA amplification and crRNA recognition, the sensitivity of a fluorescence detection method and the sensitivity of a lateral flow test strip detection method are both 10 pg, and the detection sensitivity can be further improved by increasing the RPA amplification reaction time. The method has the characteristics of convenience in operation, low time consumption, high sensitivity, strong specificity and the like, and the whole process is carried out in an environment of 35-37 DEG C. The visual detection kit provided by the invention can be used for rapidly and accurately screening a sample containing the brassicaceous vegetable phytophthora parasitica var nicotianae.

The invention discloses a dry-type electrochemical luminescence (ECL) lateral flow chip and application thereof in CRISPR-Cas detection. The dry-type ECL lateral flow chip comprises a lateral flow test strip and an electrode plate, the lateral flow test strip comprises more than one detection test strip, one electric connection test strip and at least one quality control test strip; the test strip sequentially comprises an activation sheet, a combination sheet, a detection sheet, an absorption sheet and a common sample sheet, the test strip forms radial branches taking the sample piece as the center; the electrode plate comprises an integrated closed bipolar electrode and a driving electrode; the shape of the integrated closed bipolar electrode is the same as that of the lateral flow test strip. According to the invention, the CRISPR-Cas system is freeze-dried on the test strip, so that accurate multiple detection of pathogens can be realized; according to the method, the operation process is simplified, the detection time is greatly shortened, the operation error is reduced, the mutual interference among multiple detections is reduced, and the generation of false positive results is avoided.

A point-of-care device and method for simultaneously and quantitatively assessing a magnetically-labelled analyte immobilised on a lateral flow test strip are provided. The method comprises the steps of sensing a magnetic signal from the magnetically-labelled analyte on the test strip and converting the magnetic signal into an electric signal; processing the electric signal to obtain a quantitative value of the amount of analyte; and processing the values to provide a differential assessment between the magnetically-labelled analyte. The device includes a magnetic reader with at least one magnetic sensor, such as a giant magnetoresistive sensor. The magnetic sensor can be directly or indirectly coupled to a lock-in amplifier configured to perform phase-sensitive detection and enhance the signal-to-noise ratio of weak magnetic signals. A system, microfluidics device, lateral flow immunoassay test strip and kit are also provided.

The invention relates to a nucleic acid detection platform based on an SHERLOCK technology, which is used for diagnosing CAV-2. The SHERLOCK consisting of recombinase polymerase nucleic acid pre-amplification (RPA) and CRISPR-Cas13a is mainly used for specifically recognizing a conserved sequence of CAV-2RNA (Ribonucleic Acid) through crRNA (Complementary Ribonucleic Acid) so as to activate the non-specific'side cutting activity 'of lwCas13a, so that the report RNA is degraded. A real-time fluorescent quantitative PCR (qPCR) system or a lateral flow test strip is used for collecting fluorescent signals generated by cracking of the reporter molecules, and a visual result is displayed. In addition, through HUDSON treatment, RPA-CRISPR can be amplified at room temperature without viral nucleic acid extraction. The detection limit of the method is 1.1 * 10 < 2 > copies / [mu] L, and the method has no cross reaction with other canine infectious viruses. From sample collection to result output, the whole reaction of RPA-CRISPR can be completed within two hours, and the RPA-CRISPR kit is suitable for rapid test of clinical samples and can work well in the field or in primary laboratories. The method can get rid of the limitation of a detection instrument and complex operation, and is suitable for on-site rapid batch detection. The method is preliminarily verified and further optimized and applied.

The invention provides an aquatic animal source trypanosomes visual detection primer probe set, a kit and a detection method, and belongs to the technical field of parasite detection. The invention provides a detection reagent for aquatic animal source trypanosomes. The detection reagent comprises a recombinase polymerase amplification primer pair and a probe which are designed according to an SSU rDNA gene conserved region of the aquatic animal source trypanosomes. Aiming at SSU rDNA sequence conserved regions of multiple aquatic animal trypanosomes, the invention develops a detection method and a kit based on recombinase polymerase amplification combined with a lateral flow test strip technology, and the detection method and the kit have the advantages of strong specificity, high sensitivity, short time consumption, result visualization and the like, and have low requirements on detection sites, instruments and personnel; the method is suitable for rapid detection of trypanosomes in basic laboratories and farms, and has important significance on prevention and control of trypanosomes of aquatic animals.

The invention belongs to the technical field of nucleic acid detection, and particularly relates to a POCT (point-of-care testing) micro-fluidic device for trace detection of nucleic acid from a whole blood sample and application of the POCT micro-fluidic device. The device comprises an ICP (inductively coupled plasma) paper-based preconcentrator, a plurality of PDMS (polydimethylsiloxane) micro-fluidic chip reaction chambers for nucleic acid reaction and a lateral flow test strip for signal reading. The detection process comprises the following steps: loading a whole blood sample into a preconcentrator, and carrying out blood cell separation and protein digestion; applying low direct-current voltage to realize pre-concentration of DNA; the enriched DNA is introduced into a reaction chamber for nucleic acid amplification, a nucleic acid amplification product flows into the reaction chamber through gravity for CRISPR-mediated reporter molecule activation, and the reporter molecule is combined with the mesoporous gold nano-enzyme material; finally, the reaction mixture generates a visual result on the lateral flow test strip. The device can realize rapid detection, has the advantages of high sensitivity, high specificity, low cost, simplicity and convenience in operation and the like, and is suitable for clinical and field screening application with limited resources.

The present disclosure relates to a device for performing a lateral flow test on a liquid sample, comprising: a test strip which comprises: a membrane having a first capturing reagent disposed on a first surface at a test position, the first capturing reagent being configured to capture an analyte in the liquid sample; a sample pad disposed on the first surface at a first end of the membrane spaced apart from the test position, the sample pad being configured to receive the liquid sample; and a conjugate pad disposed on the first surface between the sample pad and the test position, the conjugate pad having deposited thereon a catalytic labelling reagent, the catalytic labelling reagent being configured to chemically bind to the analyte; an activation layer disposed over the membrane arranged to contact the first surface upon activation, the activation layer having deposited thereon at least a component of a catalyst substrate, the catalyst substrate being capable of chemically reacting with the catalytic labelling reagent upon activation; and a support structure configured, when the device is in an inactive state, to affix the test strip and the activation layer such that the activation layer is separated from the membrane.

A lateral flow test strip for detecting the presence or absence of a first target analyte within a sample is described. The lateral flow test strip comprises: an input configured to receive the sample; a conjugate pad comprising a conjugate biomolecule configured to bind to a first target analyte in the sample; a membrane comprising one or more test zones, wherein a first test zone comprises a first soluble coloured compound substantially co-located with a first non-visible capture biomolecule, wherein the first non-visible capture biomolecule is attached to the membrane and is configured to bind to the first target analyte, thereby immobilising the first labelled conjugate assembly at the first test zone, and wherein the first soluble coloured compound is configured to be removed from the first test zone by a fluid flow of the sample and transported along the lateral flow test strip.

A method, apparatus and system that illuminates one face of a lateral flow assay test strip or test strip assembly with light of selected wavelengths and intensities and measures the resulting light intensities at the opposite face at the test stain line region and adjacent regions, in order to determine the value of analyte concentration over an extremely wide range, including values too small to produce a visible test stain line.

The present disclosure relates to a device for lateral flow testing of a liquid sample, the device comprising: a test strip comprising: a membrane having a first capture reagent disposed on a first surface at a test location, the first capture reagent configured to capture an analyte in the liquid sample; a sample pad disposed on the first surface at a first end of the membrane spaced apart from the test location, the sample pad configured to receive a liquid sample; and a conjugate pad disposed on the first surface between the sample pad and the test location, the conjugate pad having deposited thereon a catalytic labeling reagent configured to chemically bind to an analyte; an activation layer disposed over the membrane, arranged to contact the first surface upon activation, the activation layer having deposited thereon at least one component of a catalyst substrate capable of chemically reacting with a catalytic labeling reagent upon activation; and a support structure configured to attach the test strip and the active layer when the device is in an inactive state such that the active layer is separated from the membrane.

The present invention provides a device for detecting a substance to be analyzed in a fluid sample, the device comprising: a housing having an upper card and a lower card, the lower card being provided with a support structure for supporting a lateral flow test element, the lateral flow test element being provided for detecting a substance to be analyzed in a liquid sample, the supporting structure comprises an applying pad for receiving a liquid sample, the supporting structure comprises a supporting mechanism for supporting the whole sample applying pad, and the partial structure for supporting the sample applying pad is inclined.

The application discloses a lateral test strip for detecting C-reactive protein and application thereof, and the test strip comprises a bottom plate and a sample pad, a combination pad, a nitrocellulose membrane and a water absorption paper which are sequentially stacked on the bottom plate; the combination pad is loaded with AIE fluorescent microspheres; the AIE fluorescent microspheres are composed of P(St-MMA) microspheres coated with AIE materials after hydrolysis and carboxylation; and anti-C-reactive protein antibodies are connected to the AIE fluorescent microspheres through a covalent bond. The surface coating amount of the AIE fluorescent microspheres is increased by 1.6-2.3 times, and the fluorescence intensity per unit mass is increased by about 40-60%. The increase of the coating amount makes the density of the light-emitting core higher, the overall quantum yield is significantly improved, and the uniformity of the fluorescence signal and the detection sensitivity are enhanced. The characteristics are particularly prominent in the detection of CRP protein in the chromatographic test strip, and the detection limit can be effectively reduced, the T / C signal ratio can be improved, and the reaction time can be shortened.

The sandwich lateral flow assay devices, kits, systems and methods described herein include antibody-conjugated ultra-large particles that bind to target analytes in a sample and are retained upstream of a capture zone when a fluid sample is applied to a lateral flow test device. Embodiments of the antibody-conjugated ultra-large particles allow for accurate determination of analyte concentrations in a sample, including analytes present at high and very high concentrations. The lateral flow assays of the present disclosure can address the disadvantages associated with the hook effect of sandwich lateral flow assays by eliminating the phase of the dose response curve at which the signal intensity decreases.

The invention relates to neurodegenerative disorders, and the diagnosis and / or prognosis of neurodegenerative disorders in a test subject using a lateral flow test, or the like. The invention also relates to detecting diagnostic and prognostic biomarkers in a range of various patient sample types for diagnosing and / or prognosing neurodegenerative disorders, such as Alzheimer's disease. The invention further provides biomarker detection methods, and apparatus and apparatuses for diagnosing and prognosing neurodegenerative disorders, and methods of treating patients diagnosed or prognosed with a neurodegenerative disorder. The invention also extends to detection of biomarkers and / or screening in pre-symptomatic subjects, for early diagnosis, to enable disease prevention or intervention.

The present disclosure discloses a universal aptamer-based colloidal gold lateral flow test strip for detecting small-molecule substances and belongs to the fields of analytical chemistry, medicine, environment, food safety detection, nano-biosensing and the like. An AuNPs@poly-DNA probe is used for rapidly and sensitively capturing an aptamer, streptavidin sprayed in a test zone and streptavidin-biotin-DNAc sprayed in a control zone do not need to be changed, and another substance can be detected only by changing a nucleic acid chain part of the AuNPs@poly-DNA probe. A universal colloidal gold lateral flow test strip, which is rapid, sensitive and low in cost, has been developed. The test strip method for detecting small-molecule substances is simple, convenient and rapid, and can be used for detection at any time. Only a test solution is needed to be added into a sample port and the test strip is completely developed after 5 min, thus an experiment result can be observed and the detection efficiency can be greatly improved. Qualitative analysis can be conducted by naked eyes, and quantitative analysis can be conducted by a colloidal gold test strip quantitative analyzer.

A system (10) for determining the validity of the results of a lateral flow test, the system comprising a lateral flow stick (180a, 180b, 180c). The lateral flow stick (180a, 180b, 180c) comprises: a sample pad (310); a conjugate pad (315) comprising antibody-treated gold particles; and a porous membrane (320) for receiving a capillary flow of a milk sample. The porous membrane (320) comprises: a test line (360) treated with a progesterone reference, the progesterone reference binding to the antibody-treated gold particles of the milk sample and thereby causing the test line (360) to change color when exposed to milk having a progesterone level below a threshold limit; and a control line (370) treated with an antibody reference binding to an antibody and thereby causing the control line (370) to change color when exposed to milk comprising antibody-treated gold particles. The system also includes a sensor (210) and a control unit (150), the control unit being configured to: obtain a reference hue (380) of the control line (370); determine a current hue of the control line (370) based on an image captured by the sensor (210) during the ongoing lateral flow test; compare the reference hue (380) of the control line (370) with the current hue of the control line (370); and indicate the validity of the lateral flow test performed on the lateral flow stick (180a, 180b, 180c) based on the comparison performed.

The invention discloses a multi-channel sample detection device and method based on lateral flow test strips. The multi-channel sample detection device comprises a sample pad, an upper pressure fixing disc, a sample distribution disc, a hydrophilic film and a test strip distribution disc, the sample distribution disc is provided with a sample distribution area and a sample distribution runner; the sample pad is arranged in the sample distribution area; the hydrophilic film is adhered to the back surfaces of the sample pad and the sample distribution disc; the test strip distribution disc sleeves the periphery of the sample distribution disc, and the test strip distribution disc is a test strip area; and the upper pressing fixed disc is pressed above the periphery of the sample distribution disc and above the test strip distribution disc. A liquid sample flows to the corresponding test strips in the test strip distribution plate through the plurality of sample distribution runners of the sample distribution plate for detection, external power and complex equipment are not needed, and the liquid sample can be detected only through micro-runner optimization, structural design and capillary force effect. The liquid sample to be detected can be quickly, uniformly and automatically distributed in a plurality of detection flow channels, so that the consistency, reliability and operation convenience of a multi-channel detection result are effectively improved.

The present invention relates to the technical field of allergen detection, and discloses a high-sensitivity detection reagent strip, a preparation method, and its application, comprising a shell, the shell comprising at least a sample addition hole, a liquid addition hole, and a display area; a lateral flow test strip is provided inside the shell; the lateral flow test strip comprises a bottom structure, the bottom structure is provided with a marker flow area and a sample flow area; wherein the marker flow area is located above or below the sample flow area; the sample flow area and the marker flow area each comprise at least one sample pad, and a plurality of the sample flow areas and the marker flow areas are physically isolated from each other. The beneficial effects of the present invention are: it can realize combined immunoassay and quantitative detection, solves the problem that the traditional mode cannot be quantified or is too time-consuming, and further increases the sensitivity of the product due to the increase in the sample incubation process.

The invention provides a double-recognition probe template for diagnosis or / and auxiliary diagnosis of acute myocardial infarction. The invention provides a double-recognition probe for diagnosis or / and auxiliary diagnosis of acute myocardial infarction. The invention also provides a kit for diagnosis or / and auxiliary diagnosis of acute myocardial infarction. The invention further provides a double-target miRNA single-tube detection method based on combination of a double-recognition probe triggered RCA-CRISPR technology, and the double-target miRNA single-tube detection method is used for detecting the target miRNA by adopting the kit and adopting single-tube method CRISPR-Cas12a fluorescence / lateral flow chromatography. Wherein the target miRNA is miRNA-1 (micro Ribonucleic Acid) and / or miRNA-208 (micro Ribonucleic Acid). According to the invention, high-sensitivity detection of double targets of miRNA-1 and miRNA-208 is realized, the sensitivity is improved by 2 orders of magnitude compared with that of a standard method, and the detection time is greatly shortened; the fluorescence spectrophotometer is used for carrying out accurate quantitative detection, the macroscopic instant detection can be realized through the lateral flow test strip, and the kit is suitable for different detection scenes, especially for basic laboratories with limited resources or on-site rapid detection.

A sample test cassette includes an inlet configured to introduce a sample liquid into the sample test cassette; an elongate channel configured to receive an elongate lateral flow test strip and configured with a first end that is configured to be in liquid communication with the inlet; and a mechanical transport system that is an integral part of the sample test cassette and is configured to generate a flow of the sample liquid from outside of the inlet and towards the first end of the elongate channel.

A rapid detection reagent plate for biological samples and its preparation method, and a method for promoting the release of marker on a lateral flow test strip are provided. The test reagent device (100) includes an upper cover (101), a lower plate (102), and a test strip (1) assembled between the upper cover (101) and the lower plate (102). The test strip (1) includes a sample pad (2), a label binding pad (3), a test pad (4) and an absorbent pad (5) superposed on each other in sequence, the sample pad (2), the label binding pad (3), the test pad (4) and the absorbent pad (5) are all adhered to a bottom card (6), and the label binding pad (3) is treated in advance with a binding pad treatment solution which includes a saccharide, a polymer and a surfactant. By means of pre-treatment with the label binding pad treatment solution, release of the marker on the test strip can be effectively promoted, and the background of the test strip after test is completed is clean, such that an operator can read a test result on the test pad easily.

This invention belongs to the field of medical testing technology, specifically relating to a lateral flow test strip for ultra-high sensitivity detection of pathogens and its application. The lateral flow test strip provided by this invention uses polydopamine nanoparticles modified with bacterial antibodies as signal probes. Multiple detection lines are set on the NC membrane, and the detection lines are obtained by scribing with a bacterial antibody solution as ink. This invention replaces Au NPs in classic LFIAs with PDA NPs. Relying on the high extinction coefficient, affinity for bacteria, and stability of antibody binding of PDA NPs, a two-order-of-magnitude improvement in sensitivity is achieved when using LFIAs to detect bacteria. Simultaneously, utilizing the high extinction coefficient of PDA NPs, their signals can be identified and statistically analyzed by ImageJ software even when invisible to the naked eye. Combined with multiple test strips, this improves target capture efficiency, thereby achieving highly sensitive detection of pathogenic bacteria.

The present application discloses a vector for preparing a DNA detection probe, a construction method thereof and an application thereof. The vector is a Pt / Ti3C2T x nanoparticle, which is prepared by reducing and loading platinum nanoparticles using the two-dimensional material Ti3C2T x without the need to additionally add a reducing agent. The Pt / Ti3C2T x nanoparticle has a relatively dark initial color and has good peroxidase-like catalytic activity. Therefore, it can be introduced as a bifunctional signal tag into a lateral flow chromatography biosensor, and this signal tag adopts an aggregation signal amplification technique and a catalytic signal amplification technique to improve the sensitivity and accuracy of the lateral flow test strip biosensor. In addition, based on the Pt / Ti3C2T x nanoparticle as a signal carrier, a probe is prepared by labeling SH-DNA through Pt-S bonds, which can be applied to the detection of HIV-DNA, and can obtain detection results earlier, which is beneficial to the treatment of patients and the effective control of the virus.

Time sensitive biopharmaceutical processing updates can be implemented using lateral flow devices to test on-site during manufacturing. Lateral flow testing for an analyte such as, for example, impurities (e.g., host cell proteins), during manufacturing of a biopharmaceutical drug product is completed within twenty-five minutes of collecting a sample from the manufacturing line. That is, all sample preparation, such as adding reagent or diluting the sample, contacting the prepared sample to a lateral flow device, and detecting presence or absence of the analyte is completed within twenty-five minutes (e.g., 20 minutes, 18 minutes, 15 minutes) from collection such that processing adjustments during manufacturing can be implemented.