67 results about "Lateral flow test" patented technology

The invention discloses a method and a kit for visually detecting phytophthora parasitica var nicotianae of brassicaceous vegetables, and particularly relates to a method and a kit for visually detecting phytophthora parasitica var nicotianae based on an RPA-CRISPR / Cas12a system. A specific RPA primer pair and crRNA for detecting the brassicaceous vegetable phytophthora parasitica var nicotianae are designed according to the difference of ITS sequences of the brassicaceous vegetable phytophthora parasitica var nicotianae and the sibling species of the brassicaceous vegetable phytophthora parasitica var nicotianae. The brassicaceous vegetable phytophthora parasitica var nicotianae is detected through double specificity of RPA amplification and crRNA recognition, the sensitivity of a fluorescence detection method and the sensitivity of a lateral flow test strip detection method are both 10 pg, and the detection sensitivity can be further improved by increasing the RPA amplification reaction time. The method has the characteristics of convenience in operation, low time consumption, high sensitivity, strong specificity and the like, and the whole process is carried out in an environment of 35-37 DEG C. The visual detection kit provided by the invention can be used for rapidly and accurately screening a sample containing the brassicaceous vegetable phytophthora parasitica var nicotianae.

The invention discloses a dry-type electrochemical luminescence (ECL) lateral flow chip and application thereof in CRISPR-Cas detection. The dry-type ECL lateral flow chip comprises a lateral flow test strip and an electrode plate, the lateral flow test strip comprises more than one detection test strip, one electric connection test strip and at least one quality control test strip; the test strip sequentially comprises an activation sheet, a combination sheet, a detection sheet, an absorption sheet and a common sample sheet, the test strip forms radial branches taking the sample piece as the center; the electrode plate comprises an integrated closed bipolar electrode and a driving electrode; the shape of the integrated closed bipolar electrode is the same as that of the lateral flow test strip. According to the invention, the CRISPR-Cas system is freeze-dried on the test strip, so that accurate multiple detection of pathogens can be realized; according to the method, the operation process is simplified, the detection time is greatly shortened, the operation error is reduced, the mutual interference among multiple detections is reduced, and the generation of false positive results is avoided.

A point-of-care device and method for simultaneously and quantitatively assessing a magnetically-labelled analyte immobilised on a lateral flow test strip are provided. The method comprises the steps of sensing a magnetic signal from the magnetically-labelled analyte on the test strip and converting the magnetic signal into an electric signal; processing the electric signal to obtain a quantitative value of the amount of analyte; and processing the values to provide a differential assessment between the magnetically-labelled analyte. The device includes a magnetic reader with at least one magnetic sensor, such as a giant magnetoresistive sensor. The magnetic sensor can be directly or indirectly coupled to a lock-in amplifier configured to perform phase-sensitive detection and enhance the signal-to-noise ratio of weak magnetic signals. A system, microfluidics device, lateral flow immunoassay test strip and kit are also provided.

A lateral flow test strip for detecting the presence or absence of a first target analyte within a sample is described. The lateral flow test strip comprises: an input configured to receive the sample; a conjugate pad comprising a conjugate biomolecule configured to bind to a first target analyte in the sample; a membrane comprising one or more test zones, wherein a first test zone comprises a first soluble coloured compound substantially co-located with a first non-visible capture biomolecule, wherein the first non-visible capture biomolecule is attached to the membrane and is configured to bind to the first target analyte, thereby immobilising the first labelled conjugate assembly at the first test zone, and wherein the first soluble coloured compound is configured to be removed from the first test zone by a fluid flow of the sample and transported along the lateral flow test strip.

The present disclosure relates to a device for lateral flow testing of a liquid sample, the device comprising: a test strip comprising: a membrane having a first capture reagent disposed on a first surface at a test location, the first capture reagent configured to capture an analyte in the liquid sample; a sample pad disposed on the first surface at a first end of the membrane spaced apart from the test location, the sample pad configured to receive a liquid sample; and a conjugate pad disposed on the first surface between the sample pad and the test location, the conjugate pad having deposited thereon a catalytic labeling reagent configured to chemically bind to an analyte; an activation layer disposed over the membrane, arranged to contact the first surface upon activation, the activation layer having deposited thereon at least one component of a catalyst substrate capable of chemically reacting with a catalytic labeling reagent upon activation; and a support structure configured to attach the test strip and the active layer when the device is in an inactive state such that the active layer is separated from the membrane.

The application discloses a lateral test strip for detecting C-reactive protein and application thereof, and the test strip comprises a bottom plate and a sample pad, a combination pad, a nitrocellulose membrane and a water absorption paper which are sequentially stacked on the bottom plate; the combination pad is loaded with AIE fluorescent microspheres; the AIE fluorescent microspheres are composed of P(St-MMA) microspheres coated with AIE materials after hydrolysis and carboxylation; and anti-C-reactive protein antibodies are connected to the AIE fluorescent microspheres through a covalent bond. The surface coating amount of the AIE fluorescent microspheres is increased by 1.6-2.3 times, and the fluorescence intensity per unit mass is increased by about 40-60%. The increase of the coating amount makes the density of the light-emitting core higher, the overall quantum yield is significantly improved, and the uniformity of the fluorescence signal and the detection sensitivity are enhanced. The characteristics are particularly prominent in the detection of CRP protein in the chromatographic test strip, and the detection limit can be effectively reduced, the T / C signal ratio can be improved, and the reaction time can be shortened.

The invention relates to neurodegenerative disorders, and the diagnosis and / or prognosis of neurodegenerative disorders in a test subject using a lateral flow test, or the like. The invention also relates to detecting diagnostic and prognostic biomarkers in a range of various patient sample types for diagnosing and / or prognosing neurodegenerative disorders, such as Alzheimer's disease. The invention further provides biomarker detection methods, and apparatus and apparatuses for diagnosing and prognosing neurodegenerative disorders, and methods of treating patients diagnosed or prognosed with a neurodegenerative disorder. The invention also extends to detection of biomarkers and / or screening in pre-symptomatic subjects, for early diagnosis, to enable disease prevention or intervention.

The present disclosure discloses a universal aptamer-based colloidal gold lateral flow test strip for detecting small-molecule substances and belongs to the fields of analytical chemistry, medicine, environment, food safety detection, nano-biosensing and the like. An AuNPs@poly-DNA probe is used for rapidly and sensitively capturing an aptamer, streptavidin sprayed in a test zone and streptavidin-biotin-DNAc sprayed in a control zone do not need to be changed, and another substance can be detected only by changing a nucleic acid chain part of the AuNPs@poly-DNA probe. A universal colloidal gold lateral flow test strip, which is rapid, sensitive and low in cost, has been developed. The test strip method for detecting small-molecule substances is simple, convenient and rapid, and can be used for detection at any time. Only a test solution is needed to be added into a sample port and the test strip is completely developed after 5 min, thus an experiment result can be observed and the detection efficiency can be greatly improved. Qualitative analysis can be conducted by naked eyes, and quantitative analysis can be conducted by a colloidal gold test strip quantitative analyzer.

The invention discloses a multi-channel sample detection device and method based on lateral flow test strips. The multi-channel sample detection device comprises a sample pad, an upper pressure fixing disc, a sample distribution disc, a hydrophilic film and a test strip distribution disc, the sample distribution disc is provided with a sample distribution area and a sample distribution runner; the sample pad is arranged in the sample distribution area; the hydrophilic film is adhered to the back surfaces of the sample pad and the sample distribution disc; the test strip distribution disc sleeves the periphery of the sample distribution disc, and the test strip distribution disc is a test strip area; and the upper pressing fixed disc is pressed above the periphery of the sample distribution disc and above the test strip distribution disc. A liquid sample flows to the corresponding test strips in the test strip distribution plate through the plurality of sample distribution runners of the sample distribution plate for detection, external power and complex equipment are not needed, and the liquid sample can be detected only through micro-runner optimization, structural design and capillary force effect. The liquid sample to be detected can be quickly, uniformly and automatically distributed in a plurality of detection flow channels, so that the consistency, reliability and operation convenience of a multi-channel detection result are effectively improved.

The invention provides a double-recognition probe template for diagnosis or / and auxiliary diagnosis of acute myocardial infarction. The invention provides a double-recognition probe for diagnosis or / and auxiliary diagnosis of acute myocardial infarction. The invention also provides a kit for diagnosis or / and auxiliary diagnosis of acute myocardial infarction. The invention further provides a double-target miRNA single-tube detection method based on combination of a double-recognition probe triggered RCA-CRISPR technology, and the double-target miRNA single-tube detection method is used for detecting the target miRNA by adopting the kit and adopting single-tube method CRISPR-Cas12a fluorescence / lateral flow chromatography. Wherein the target miRNA is miRNA-1 (micro Ribonucleic Acid) and / or miRNA-208 (micro Ribonucleic Acid). According to the invention, high-sensitivity detection of double targets of miRNA-1 and miRNA-208 is realized, the sensitivity is improved by 2 orders of magnitude compared with that of a standard method, and the detection time is greatly shortened; the fluorescence spectrophotometer is used for carrying out accurate quantitative detection, the macroscopic instant detection can be realized through the lateral flow test strip, and the kit is suitable for different detection scenes, especially for basic laboratories with limited resources or on-site rapid detection.

A sample test cassette includes an inlet configured to introduce a sample liquid into the sample test cassette; an elongate channel configured to receive an elongate lateral flow test strip and configured with a first end that is configured to be in liquid communication with the inlet; and a mechanical transport system that is an integral part of the sample test cassette and is configured to generate a flow of the sample liquid from outside of the inlet and towards the first end of the elongate channel.

This invention belongs to the field of medical testing technology, specifically relating to a lateral flow test strip for ultra-high sensitivity detection of pathogens and its application. The lateral flow test strip provided by this invention uses polydopamine nanoparticles modified with bacterial antibodies as signal probes. Multiple detection lines are set on the NC membrane, and the detection lines are obtained by scribing with a bacterial antibody solution as ink. This invention replaces Au NPs in classic LFIAs with PDA NPs. Relying on the high extinction coefficient, affinity for bacteria, and stability of antibody binding of PDA NPs, a two-order-of-magnitude improvement in sensitivity is achieved when using LFIAs to detect bacteria. Simultaneously, utilizing the high extinction coefficient of PDA NPs, their signals can be identified and statistically analyzed by ImageJ software even when invisible to the naked eye. Combined with multiple test strips, this improves target capture efficiency, thereby achieving highly sensitive detection of pathogenic bacteria.

Time sensitive biopharmaceutical processing updates can be implemented using lateral flow devices to test on-site during manufacturing. Lateral flow testing for an analyte such as, for example, impurities (e.g., host cell proteins), during manufacturing of a biopharmaceutical drug product is completed within twenty-five minutes of collecting a sample from the manufacturing line. That is, all sample preparation, such as adding reagent or diluting the sample, contacting the prepared sample to a lateral flow device, and detecting presence or absence of the analyte is completed within twenty-five minutes (e.g., 20 minutes, 18 minutes, 15 minutes) from collection such that processing adjustments during manufacturing can be implemented.

A system for monitoring and controlling application of milk samples to lateral flow test sticks. The system includes a milk dosing assembly with a needle that moves between an application position contacting a test stick and a retracted position. A moveable structure holds and positions the dosing assembly, while a drive unit controls the movement. A controller determines and maintains proper spacing between the needle tip and test stick by comparing measured distances with predetermined values and making adjustments as needed. The system enables accurate and automated application of milk samples for biomarker testing while preventing sample cross-contamination and ensuring proper sample delivery to the test area.

The invention belongs to the technical field of plant virus detection, and particularly relates to an RT-RPA-LFD method capable of rapidly and visually detecting three pathogens of sugarcane mosaic disease at the same time and application of the RT-RPA-LFD method. The invention relates to an RT-RPA-LFD primer group capable of simultaneously, rapidly and visually detecting three pathogens of sugarcane mosaic disease. The RT-RPA-LFD primer group comprises a primer group I, a primer group II and a primer group III, wherein the primer group I comprises an SrMV primer pair and an SrMV probe; the primer group II comprises an SCSMV primer pair and an SCSMV probe; and the primer group III comprises an SCMV primer pair and an SCMV probe. According to the multiple RT-RPA-LFD detection method disclosed by the invention, complicated RNA extraction, cDNA reverse transcription, gel electrophoresis and the like are not needed in the whole process, and multiple detection results can be observed by naked eyes within 5 minutes through a lateral flow test strip only by incubating at the constant temperature of 37 DEG C for 10 minutes. The detection system has the characteristics of rapidness, high efficiency, high sensitivity, strong specificity and no dependence on laboratory equipment, is suitable for on-site large-scale detection of field samples and sugarcane introduction materials, and greatly improves the detection efficiency.

The invention is directed to a method for producing a roll (1) or sheet of membrane units (2) for a membrane product (3) such as a lateral flow test from a roll (6) or sheet of membrane material (7), by means of a primary production arrangement (9), wherein the roll (6) or sheet of membrane material (7) is processed into a roll (1) or sheet of membrane units (2) in a primary processing routine, wherein in the primary processing routine, for generating the membrane units (2), a fluidic structure (11), in particular a hydrophobic structure, for defining fluid flow through the membrane material (7) is introduced into the membrane material (7) by means of a processing tool (12), wherein an evaluation routine is performed by means of an evaluation arrangement (15) comprising a sensor arrangement (16) and an evaluation control (17). It is proposed that in the evaluation routine, evaluation images (18) of the fluidic structures (11) of the membrane units (2) are generated by means of the sensor arrangement (16) and evaluation data (19) are generated by means of the evaluation control (17) and that the evaluation data (19) represent the deviation in predefined geometrical properties of the fluidic structure (11) in the respective evaluation image (18) with respect to the fluidic structure (11) in a reference image (21).

The present disclosure relates to an electronic reader for reading a result from a lateral flow test device. The electronic reader comprises: a receiving portion for receiving the lateral flow test d

The invention belongs to but not limited to the technical field of human and animal health diagnostics, and discloses a binding molecule for acute infection diagnosis adapted to a point-of-care (POC) scene, an acute infection diagnosis system and a kit. The binding molecule is an antibody, a nano antibody or an aptamer (preferably not combined with IgM) which is specifically combined with a J chain of dIgA, steric hindrance and conformation rearrangement are avoided by combining the J chain, and the flexibility of a compound is kept so as to efficiently combine with an antigen; the diagnostic system and the kit are adapted to POC scenarios (such as lateral flow test strips). The problems that POC deployment is difficult, RAT sensitivity is low and dIgA detection efficiency of CSC is low in existing NAT are solved, and the kit has the advantages of being low in cost, high in specificity and good in stability, can be used for rapid diagnosis of multiple acute infections such as measles and syphilis, fills up related technical gaps and has high commercial value.

A lateral flow test kit includes a cassette and a swab. The cassette comprises a vial of liquid closed by seal; a test strip; a passage leading from the exterior of the cassette to the vial; and a conduit communicating between the vial and the test strip. The swab can be inserted along the passage to cause a tip of the swab to enter the vial and release liquid from the vial. The liquid flows past a head of the swab to extract a sample and deliver it through the conduit 34 to the test strip. The passage may comprise an obstruction, which requires the user to push the swab past it with a force sufficient to penetrate the seal. The obstruction may also prevent removal of the used swab from the cassette. A distal end of the passage may be curved to deflect the tip of the swab towards the seal at a more favourable angle.

A method for determining a target analyte level comprising: obtaining test data of a test region of a lateral flow device, test data comprising test data values of a light parameter of light reflected from test region; dividing test data into test data sub-groups; obtaining light control data of a light control region, light control data comprising light data values of the light parameter from the light control region; dividing light control data into light control data sub-groups, each light control data sub-group having a corresponding test data sub-group; for at least some of the test data sub-groups, comparing test data values of a given test data sub-group with light data values of the corresponding light control data sub-group, and correcting, if any variations are determined, test data values; and comparing corrected test data with predetermined correlation data correlating light data values with different target analyte levels.

The invention belongs to the field of molecular biological detection, and relates to a kit, in particular to a double-virus visual detection kit based on a CRISPR / Cas9-LFD (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR associated protein 9-lateral FD) technology, a detection method and application. According to the invention, a CRISPR-Cas9 technology and a lateral flow test strip technology are combined, a dual visual detection system for PNRSV and ApNMV viruses based on a CRISPR / Cas9-LFD technology is developed, the developed detection method is evaluated, and an optimal detection system is selected. A rapid, visual and cheap simultaneous visual detection technology for the PNRSV and ApNMV viruses is finally established through system optimization, large instruments are not needed, the detection sensitivity is 4.8 * 10 < 2 > copy / reaction and is 10 times that of RT-PCR, the specificity is good, and simultaneous visual detection of the two viruses can be achieved within 40 min under the condition of the constant temperature of 37 DEG C.

The invention discloses an Alternania alternana RPA (recombinase polymerase amplification) lateral flow test strip detection kit and application thereof in Bo-chrysanthemum disease detection, and belongs to the field of rapid detection of plant pathogenic fungi. The detection kit provided by the invention comprises a reagent for detecting Alt a 1, the reagent comprises a first RPA primer, and the nucleotide sequences of the first RPA primer are respectively SEQ ID NO.1 and SEQ ID NO.2; the reagent for detecting TEF1 alpha comprises an RPA primer II, and the nucleotide sequences of the RPA primer II are respectively SEQ ID NO.4 and SEQ ID NO.5; and an RPA reaction component. Compared with the prior art, the kit disclosed by the invention has the advantages that through mutual verification of double targets, the detection specificity and accuracy are improved, false negative caused by gene variation of a single target is avoided, and the identification capability on a complex sample is improved.

The invention discloses an anti-African swine fever p72 protein single-chain antibody as well as a nucleic acid molecule, a recombinant expression vector, a preparation method and application thereof, and belongs to the technical field of detection of pathogenic microorganisms of livestock. The single-chain antibody is formed by sequentially connecting a heavy chain variable region, a connecting peptide and a light chain variable region, and the amino acid sequence of the single-chain antibody is shown as SEQ ID NO: 1. The invention also provides a nucleic acid molecule for coding the single-chain antibody as well as a recombinant expression vector and a host cell of the nucleic acid molecule. The single-chain antibody with high affinity and high specificity is obtained by screening through a phage display technology, is only combined with the African swine fever virus p72 protein, does not react with common swine virus protein, is small in molecular weight and good in stability, is suitable for various detection scenes such as immunodetection and histochemical analysis, and has good application prospects. The kit can be used for developing products such as a rapid diagnostic kit and a lateral flow test strip, and is suitable for detecting African swine fever.

Methods and kits for the rapid detection of active coronavirus infection by specifically targeting the negative-sense RNA produced during viral replication. The method utilizes nucleic acid amplification techniques, such as RT-PCR and qPCR, with primers specific to the negative strand, which serves as a marker for active viral replication. The present disclosure also encompasses a rapid, user-friendly lateral flow test based on CRISPR-Cas13a technology that targets the negative-sense viral RNA. This LFT employs guide RNAs specific to the negative strand and a reporter system to visually indicate the presence of actively replicating viruses, thereby distinguishing active infections from the presence of non-replicating viral remnants. The methods and kits offer a more accurate means of determining infectivity, guiding public health measures, and monitoring antiviral therapies.

The present invention generally relates to a system and method for detecting multiple analytes in a blood sample. The present invention relates to an advanced cartridge specially designed for detecting multiple analytes in a blood sample. The cartridge integrates a series of components to process blood samples, encompassing the overall process from sample reception to plasma separation. Subsequently, the plasma is precisely metered and guided to an incubation process, so that the plasma is mixed with a reagent under controlled conditions and incubated. And after incubation is completed, analyzing the plasma by using a lateral flow test strip so as to detect and quantify various target analytes. The card box system is particularly suitable for instant detection, a simple and automatic scheme is provided for executing complex detection including immunoassay and electrochemical analysis, and the accuracy and efficiency of detection are remarkably improved.

The invention encompasses a real time, point of care, diagnostic system, including a lateral flow test cassette, a data reader, and an application for processing test results and methods of diagnosis of a disease or virus.

The present application belongs to the field of analytical chemistry detection, and particularly relates to a nucleic acid lateral flow chromatographic test strip, a preparation method and application thereof, and aims to solve the technical defects of traditional lateral flow test strip, such as single target, low quantitative precision and insufficient sensitivity. The test strip is assembled from a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a PVC bottom plate, the combination pad is loaded with Au nanoparticle modified nucleic acid reporter probe, and the C line probe and the T line probe are fixed on the NC membrane; the test strip is targeted recognition mediated by strand displacement reaction, different types of targets are converted into M-DNA with the same sequence, after exponential amplification of the signal, quantitative detection is completed by using test strip color development combined with sampling and software analysis. The sensitivity can be as low as 0.18 pM for different analytes, and good specificity and stability are still maintained in complex matrix such as serum, and it is suitable for rapid quantitative analysis of multiple types of targets in the scene of instant detection.

A lateral flow test strip reader for reading an output of a lateral flow assay to determine a presence or absence of a target in a sample includes: a housing having a lateral flow test strip receptacle for receiving a lateral flow test strip therein, the lateral flow test strip receptacle defining a test region and a control region for a lateral flow test strip; a light source that generates an excitation light beam; at least one lens for optically expanding the excitation light beam in a direction across the test region and the control region such that the excitation light beam is configured to simultaneously impinge and excite both the test region and the control region; and an optical detector configured to simultaneously detect an image comprising emission signals from the test region and the control region.

The application belongs to the field of electrochemistry, and relates to tumor protein P53 gene detection, in particular to a label-free double-color test strip, a kit and a preparation method thereof for tumor protein P53 gene detection. The application belongs to a novel LFA method, which combines DNA hybridization chain reaction with the electrostatic adsorption principle of test line / control line, selects common and cost-effective bovine serum albumin as the test line, utilizes the difference in the adsorption capacity of BSA to double-stranded DNA and nano-gold, and the different flow rates of different molecules on the NC membrane to complete the detection of p53 at different concentrations. The test line is constructed by positively charged PDDA to ensure the effectiveness of the LFA. Meanwhile, the detection principle of a liquid colorimetric sensor is combined, and the double-color development greatly reduces the detection limit of the test strip. The sensor is label-free throughout, and has high analytical performance in terms of sensitivity, selectivity and practicability. The p53 gene is detected on the lateral flow test strip for the first time.

The present application belongs to the field of biological detection, and particularly relates to a field visual rapid detection method for citrus Huanglongbing. The present application is based on MIRA-lateral flow test paper, takes the outer membrane protein (OMP) of Asian species of Huanglongbing bacteria of citrus as a target, designs and selects specific primers and probes, and prepares dry powder microspheres together with MIRA reaction enzymes, mixes the field rapid extraction plant DNA lysate, dry powder microspheres and Buffer microspheres in a closed chamber to react, adopts a miniature temperature control device to complete amplification under the condition of 42 DEG C for 20 minutes, and directly realizes visual interpretation of the associated test paper strip. The detection method has no cross reaction with other citrus pathogenic bacteria, the sensitivity test can detect the DNA concentration lower limit of 6.9x10 ‑3 ng / μL, the detection device is small in size and convenient to carry, the whole process does not need professional operation or precise instruments, the detection can be completed within 30 minutes, and the detection method is suitable for rapid detection of citrus Huanglongbing by grass-roots agricultural technicians, planting enterprises and fruit farmers.