RPA-LFA (recombinase polymerase amplification-lateral flow assay) detection kit for detecting brucella and use method of kit

Abstract

The invention discloses an RPA-LFA (recombinase polymerase amplification-lateral flow assay) detection kit for detecting brucella. The kit comprises (1) a sample tretament solution, (2) a detection reaction solution, (3) a dry powder tube, (4) a lateral flow test strip, (5) a positive control reaction solution, (6) a negative control reaction solution and (7) a sample treatment solution. The kit combines simple treatment of samples, RPA and LFA technologies, no simultaneous equipment is required, and all that is required for detection is to boil the samples, so that the kit is very suitable tobe used on site or in fields, can detect and diagnose brucella in veterinarian clinics or farms, has great clinical application value for diagnosis and control of the diseases and has high detectionprecision and broad market prospects.

Description

technical field

[0001] The invention belongs to the technical field of medicine and biotechnology, and in particular relates to an RPA-LFD detection kit for detecting Brucella and a use method thereof. Background technique

[0002] Brucellosis, also known as Mediterranean relaxation fever, Malta fever, wave fever or undulating fever, is a zoonotic systemic infectious disease caused by Brucella, which can infect animals, such as cattle, sheep, pigs, dogs and camels, Deer and other animals. Its clinical features are long-term fever, hyperhidrosis, arthralgia, hepatosplenomegaly, etc. The chronic phase of the disease may cause damage to multiple organs and systems.

[0003] Brucella is a Gram-negative non-motile bacterium, non-capsulated (smooth type with microcapsule), positive for catalase and oxidase, absolutely aerobic, capable of reducing nitrate, intracellular Parasitic, can survive in many kinds of domestic animals. There are six species of Brucella: Brucella malta (B...