RPA-LFA (recombinase polymerase amplification-lateral flow assay) detection kit for detecting brucella and use method of kit
A detection kit, Brucella technology, applied in the fields of medicine and biology, can solve the problems of difficult to meet the detection needs, expensive, long time, etc., and achieve the effect of lowering the technical threshold, easy operation, and broad market prospects
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[0031] A kind of RPA-LFD detection kit that detects Brucella, comprises: (1) sample treatment liquid: the PBS solution of 0.1M pH7.4; (2) detection reaction liquid: the Tris-HCl of 50mM pH7.9; 100mM Potassium acetate; 2mM dithiothreitol; 3mM adenosine triphosphate; 20mM creatine phosphate; 5μg creatine kinase; 5% polyethylene glycol, 200μM deoxyribonucleoside triphosphate, primer (Primers BBRRPAF and BBRRPAB each 0.4μM, probe BBRRPAP 0.05μM), 14mM magnesium acetate; (3) dry powder tube: 3.75μg lyophilized recombinant enzyme uvsX, 750ng lyophilized recombinant enzyme uvsY, 30μg single-chain binding protein , 900ng strand displacement polymerase; (4) lateral flow test strip; (5) positive control reaction solution: 100 copies of the BvrR gene; TE buffer solution of 10mMpH8.0; (6) negative control reaction solution: 10mM pH8. 0 TE buffer solution; (7) loading treatment solution: PBST buffer solution of 0.01MpH7.3;
[0032] Among them, the forward primer is
[0033] BBRRPAF: CTCA...
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