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141 results about "Genus Brucella" patented technology
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Brucella is a genus of Gram-negative bacteria, named after David Bruce (1855–1931). They are small (0.5 to 0.7 by 0.6 to 1.5 µm), non-encapsulated, non-motile, facultatively intracellular coccobacilli.
Brucella rapid detection kit based on mass-spectrometric technique
The invention provides a kit for rapidly detecting brucella in a blood sample, relating to the technical field of fingerprint spectrum detection of proteins. According to the kit, based on the mass-spectrometric technique, a specific polypeptide mass-spectrometric model aiming at brucella detection in the blood is constructed, and a kit for carrying out the brucella detection by utilizing the mass-spectrometric technique is constructed. According to the kit provided by the invention, the brucella in the blood sample can be authenticated by utilizing a specific fingerprint spectrum, the accuracy is high, and the repeatability is good; the detection on 96 samples can be finished within 2 hours and can be finished at least 5 days in advance compared with the detection time in the conventional detection method; the detection cost is low, and the result is reliable, so that the kit has a good application prospect.
Owner:ICDC CHINA CDC
Primer, probe and reagent kit for identifying Brucella S2 vaccine strains in aerocolloid
InactiveCN104862405AIncreased sensitivityStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationBacteroidesForward primer
The invention relates to a primer, probe and reagent kit for identifying Brucella S2 vaccine strains in aerocolloid. The forward primer sequence of Brucella-RPA-LFD is shown as SEQIDNo.1, the backward primer sequence of Brucella-RPA-LFD is shown as SEQIDNo.2, and the probe sequence of Brucella-RPA-LFD is shown as SEQIDNo.3; the forward primer sequence of S2-RPA-LFD is shown as SEQIDNo.4, the backward primer sequence of S2-RPA-LFD is shown as SEQIDNo.5, and the probe sequence of S2-RPA-LFD is shown as SEQIDNo.6. The combination of the RPA-LFD primer and the probe, or the reagent kit, for identifying and diagnosing the Brucella S2 vaccine strains, provided by the invention are high in sensitivity and specificity, and can at least detect 2.5*100 cfu of Brucella or the Brucella S2 vaccine strains.
Owner:何洪彬
Low virulent strain of Brucella and vaccine thereof
ActiveCN103981139AImprove immunityImprove securityAntibacterial agentsBacterial antigen ingredientsSerum igeImmune effects
The invention relates to a low virulent strain of Brucella and a vaccine thereof. According to a vaccine strain, a coarse low virulent strain RA343 of Brucella abortus is screened through a domestication and selection technology combining antibiotics and A-factor serum. The coarse low virulent strain is remarkably improved in safety and still remains a good immune effect on the Brucella is still maintained. A Brucella vaccine prepared by using the low virulent strain is to change the current situation that Brucella vaccine-immunized animals and wild strain-infected animals are hard to differentiate, and the safety of existing vaccine is effectively improved.
Owner:CHINA INST OF VETERINARY DRUG CONTROL
Method for detection and differential diagnosis of Brucella in aerosol
The invention discloses a method for detection and differential diagnosis of Brucella in an aerosol. The method comprises the following steps: (1) acquisition of an aerosol sample; (2) extraction of genome total DNA of the aerosol sample; (3) detection: a step of carrying out PCR amplification by using specific primers and a kit; (4) establishment of a standard curve and a melting curve: a step of establishing the standard curve of pEASY-T3-VirB10 positive standard plasmid and the melting curve of an amplification system; and (5) judgment: a step of judging whether the aerosol sample contains Brucella. The invention further discloses the specific primers (as shown in SEQ ID No. 3 to 9) and the kit (composed of the specific primers, the pEASY-T3-VirB10 positive standard plasmid, an SYBRGreenI real-time fluorescent quantitative PCR reagent and ddH2O). The method provided by the invention is applicable to detection and identification of Brucella in the aerosol in a culturing farm and to detection and identification of samples like clinical blood, serum, milk and tissue.
Owner:DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
Brucella antibody immune colloidal gold detection test paper strip and preparing method thereof
The invention discloses a Brucella antibody immune colloidal-gold detection test strip, wherein, staphylococcus aureus A proteion (SPA) labeled colloidal-gold is made into the colloidal-gold pad of the colloidal-gold antibody detection test strip. L7 / L12 protein is a Brucella protein with high specificity and immunogenicity. L7 / L12 gene is cloned from bovine Brucella genome and is connected to pET-28a to construct the prokaryotic expression recombinant plasmid, then the plasmid is transferred into the Escherichia coli to express L7 / L12 protein, after the protein is purified, the purified protein is used as antigen to coat the nitrate cellulose film to be used as the check line (T line), and the check line and the colloidal-gold pad are assembled to the immune colloidal-gold detection teststrip of the invention. The test strip of the invention can be used for the detection of Brucella antibody in animal serum with strong specificity, high sensitivity, and good stability, and has considerable meaning and actual application value for the monitoring, diagnosis, purification, and control of the Brucella.
Owner:JILIN UNIV
Kit for identifying Brucella S2 vaccine strain and wild strain
InactiveCN105002173AEasy to distinguishStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationBacteroidesSequence analysis
The invention relates to a kit for identifying a Brucella S2 vaccine strain and a wild strain. The kit comprises a specific primer pair SEQ ID No.1 and SEQ ID No.2 for identifying the Brucella vaccine strain S2. Sequencing analysis is further performed on a PCR amplification product after the kit is used for distributing Brucella and other conventional bacterial strains through a PCR amplification-electrophoresis detection area, and the Brucella S2 vaccine strain in a clinic sample can be rapidly and effectively identified and diagnosed according to the sequencing result.
Owner:DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
Test strip for rapid detection of brucella
InactiveCN101362800AEasy to storeEasy to transportImmunoglobulins against bacteriaDepsipeptidesBinding siteField tests
The invention provides a quick test dipstick used for testing a specific antigen of Brucella, which comprises a reaction membrane and a binder release liner. The reaction membrane is provided with a testing strap coated with a bp26 monoclonal antibody or bp26 polyclonal antibody and a quality control strap coated with anti-IgG; the binder release liner is coated with the bp26 monoclonal antibody which is marked by colloidal gold to be different from an antigen binding site on the reaction membrane. A membrane chromatography double antibody sandwich method is applied for detecting the specific antigen of the Brucella in a specimen. As the dipstick is adopted for test, the operation is simple, convenient, quick and concise, requiring no special equipment and facilities as well as professional training. Furthermore, the dipstick has clear and easy-identity results, simple operation and easy popularization, is applicable to matrixes, field tests of emergency on a large scale and the study of epidemiology and can aid in the infection diagnostics of the Brucella.
Owner:BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
Animal brucella antibody gold mark rapid detection kit
The invention discloses an animal brucella antibody gold mark rapid detection kit and belongs to the field of animal disease detection. The kit consists of two parts namely a card box and a test strip, wherein the card box comprises a box cover (4) and a box bottom (7), and the detection test strip is placed in the card box; a sampling hole (1) and an inspection window (2) are formed in the front side of the card box, and a sampling hole label (5), a result judging specification (6) and an information recording column (3) are printed on the front side of the box cover. According to the invention, the colloidal gold immunochromatography technology is adopted, a brucella BP26, OMP25 or OMP31 pathogen in engineering expression is used as the detection pathogen, a mouse anti-bovine IgG monoclonal antibody, a mouse anti-goat IgG monoclonal antibody or a mouse anti-swine IgG monoclonal antibody is used the capture antibody, and an indirect process detection kit is made. The animal brucella antibody gold mark rapid detection kit can be used for detecting the brucella antibody in the serum, the plasma or the whole blood of cattle, goats or swines and has the advantages of simple detection method, accurate and rapid result display and no requirement of special instruments or equipment.
Owner:LANZHOU YAHUA BIOTECH +1
Preparation method and application of brucellosis specific fusion protein antigen
ActiveCN105906714AImprove responseIncreased riskBacteriaAntibody mimetics/scaffoldsEscherichia coliSolubility
The invention discloses a brucellosis specific fusion protein antigen. The brucellosis specific fusion protein antigen is a fusion protein expressed by Escherichia coli BL21 (DE3) after inserting a fusion protein gene constructed by serially connecting amino acid sequences on dominant antigen epitopes of Brucella outer membrane proteins BP26, OMP31, OMP16 and OMP2b to pET-28b, approaches a natural protein in conformation, keeps the antigenicity and the solubility of the natural protein, and can be used as a diagnosis antigen to produce a human brucellosis antibody ELISAS kit. The kit overcomes the disadvantages of long separate culture enrichment time, easy pollution to environment and easy human infection of traditional etiological diagnosis, and also improves the severe cross reaction of traditional immunological methods. The kit has the advantages of strong specificity, good repeatability and high sensitivity, provides means for rapid, accurate and specific diagnosis of brucellosis, and provides bases for early diagnosis and early treatment of brucellosis patients.
Owner:JILIN UNIV
Animal brucella antigen colloidal gold test paper film detection reagent kit
The invention discloses an animal brucella antigen colloidal gold test paper film detection kit, which bases on immunology basic principle of antigen and antibody specific combination, utilizes colloidal gold immunity chromatography technique and silver dye reinforcement technique, uses combined golden yellow staphylococcus A protein (SPA) as gold marking antibody, uses high purity brucella LPS as peridium antigen, and utilizes goat anti-bovine IgG as quality control line to prepare colloidal gold test paper tape. The invention can accurately differentiate species of animal brucella and can synchronously detect animal brucella disease of cattle, sheep and pig species with no crossing reaction and low false negative proportion, negative and positive coincidence ratio of the invention and conventional separation and culture is 100%, detection result can be obtained in 5 min, and the kit need no assistant complicated detection instrument.
Owner:MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Brucellosis antibody competitive enzyme-linked immunosorbent assay reagent kit
ActiveCN101592661AAccurate diagnosisMeet the needs of epidemic preventionMaterial analysisAntigenPositive control
The invention aims to provide a brucellosis antibody competitive enzyme-linked immunosorbent assay reagent kit which meets the demand of epidemic prevention of animals in our country, is sensitive and specific, and can accurately diagnose brucellosis. The reagent kit is assembled by utilizing lipopolysaccharide of smooth brucella as an antigen coated ELIAS plate, using an inactivated bacterial liquid to immunize a healthy cow or artificially infect the healthy cow to prepare positive control serum and standard weak-positive serum, using serum of a healthy non-immune cow as negative control serum, using a monoclonal antibody as a competitive antibody, namely an ELIAS secondary antibody (goat anti-rat IgG), and preparing a serum diluent, a washing liquid, a substrate solution A, a substrate solution B, H2O2, and a stop buffer. The brucellosis antibody competitive enzyme-linked immunosorbent assay reagent kit is a competitive ELISA reagent kit which is sensitive and specific and can accurately diagnose the brucellosis. The reagent kit meets the demand of the epidemic prevention of the animals in our country, and performs final definite diagnosis on the brucellosis by means of laboratory diagnosis technology.
Owner:哈尔滨平河生物技术有限公司 +2
Genetic liquid phase chip for joint detection of five drastic pathogenic bacteria and detection method thereof
InactiveCN101560557AMicrobiological testing/measurementMicroorganism based processesYersinia pestisPathogenic bacteria
The invention discloses a genetic liquid phase chip for rapid detection of five drastic pathogenic bacteria of bacillus anthracis, yersinia pestis, brucella bacteria, francisella tularensis and burkholderia pseudomallei. The liquid phase chip for detecting the five drastic pathogenic bacteria has the advantages of large flux, less required sample capacity, strong specificity, high sensitivity, accuracy, high efficiency, and the like.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE
PCR method for quickly detecting brucella in milk sample
InactiveCN101659995APracticalHigh sensitivityMicrobiological testing/measurementMicroorganism based processesMilk samplePcr method
The invention discloses a PCR method for quickly detecting brucella in a milk sample, relating to a gene detection technology of pathogens of zoonotic infectious diseases and being applicable to qualitative detection of brucella. The PCR method consists of the components of a standard positive template, a PCR reaction solution, a brucella BCSP31 gene specific primer and a negative quality-controlstandard sample. The invention has the advantages of quick detection speed, good specificity, high sensitiveness, simple using steps, high repeatability, and capability of replacing the traditional pathogenic detection method.
Owner:JILIN UNIV
Test paper strip for rapidly detecting brucellosis antibody
ActiveCN101363859AEasy to storeEasy to transportMaterial analysisPolyclonal antibodiesCell Membrane Proteins
The invention provides a test strip for rapid detection of a Brucella specific antibody, which comprises a reaction film and a conjugate release pad. The reaction film has a detection band simultaneously coated with Brucella specific antigen bp26, and a quality control band of polyclonal antibody coated with Brucella outer-membrane protein bp26. The conjugate release pad is coated with colloidal golden labeled Brucella outer-membrane protein bp26. A membrane chromatography double antigen sandwich method is adopted to detect the Brucella specific antibody in a specimen. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, large scale site detection of an accident and epidemiological investigation, and has auxiliary effect on the diagnosis of Brucella infection.
Owner:辽宁迪浩生物科技有限公司
ELISA detection kit for bovine Brucella
ActiveCN105445473AEasy to operateReduce use costBiological material analysisBiological testingSerum igeElisa kit
The invention provides an ELISA detection kit for bovine Brucella, belonging to the technical field of protein engineering. In the kit provided by the invention, the mixed antigen of the outer membrane proteins OMP19, OMP22 and OMP28 of bovine Brucella is used as an envelope antigen of the ELISA kit; the concentration of each antigen is 1mu g / ml, and the antigens are mixed in a volume ratio of 1:1:1 to obtain the mixed antigen; the serum dilution is 1:200, and the dilution of the enzyme-labeled secondary antibody is 1:20,000; and the bovine Brucella is detected through an indirect ELISA method. The kit provided by the invention has the characteristics of high specificity, high sensitivity, high accuracy, easiness in operation and good reproducibility while the market application prospect is good.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE
Brucella molecule marking and virulence deletion attenuated vaccine and preparation method
InactiveCN101185756AWide application of practical valueAntibacterial agentsBacterial antigen ingredientsVirulent characteristicsVaccine Immunogenicity
The invention relates to a Brucella vaccine, in particular to the molecular marker and virulence gene deletion of Brucella vaccine strain. The study uses luciferase modified gene (Luc NF plus) to replace the partial fragment of Bp26 gene of Brucella attenuated vaccine S19 strain by constructing suicide plasmid and adopting the method of targeted homologous recombination (gene targeting), so as to damage the expression of the immunogenicity protein BP26 and construct the gene deletion mutant strain Delta S19-1 of the Brucella Bp26. The BMP18 protein is one of the main virulence factors of Brucella. The invention adopts the same method to exclude the Bmp 18 gene of Delta S19-1, so as to lead the Delta S19-1 not to express the Bmp 18 protein and the Brucella virulence gene deletion mutant strain Delta S19-2 is constructed. The invention solves the problems that the conventional Brucella vaccine can not distinguish between the artificial immunization and the wild bacteria infection of people and animals, the virus is strong and the vaccine is easy to cause the illness of inoculated people and animals. The invention has important significance and practical application value of the monitoring, diagnosis, purification and all the controls of Brucella.
Owner:MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Brucellosis indirect enzyme-linked immunosorbent assay antibody detection kit
The invention aims to provide a brucellosis indirect enzyme-linked immunosorbent assay antibody detection kit which has moderate cost and short reaction time and is closely combined with the requirements of actual detection and general survey operations in China. The brucellosis indirect enzyme-linked immunosorbent assay antibody detection kit is assembled by using a purified lipopolysaccharide mixture of smooth type brucella and rough type brucella as antigen coated wells, using S1119.3 immune and healthy cattle to prepare positive control serum and standard weak positive serum, using healthy and non-immune cattle serum as negative control serum, using protein AG which is labeled by enzyme and is a receptor in combination with mammal Fc segment as enzyme labeling combo, and together taking serum diluent, a cleaning solution, a substrate solution A, a substrate solution B, H2O2 and a stopping solution as coordination. The invention establishes a rapid, sensitive, special and accurate method suitable for brucellosis diagnosis and epidemiological survey, has objective result judgment, simple and convenient operation, and is suitable for both large-scale screening tests and final confirmed diagnosis of the brucellosis.
Owner:张晓艳
Recombinant brucella expressing VP1 gene of O-type foot-and-mouth disease virus and method for producing vaccines thereof
ActiveCN101979502AImprove securityStable genetic traitsAntibacterial agentsBacterial antigen ingredientsImmune effectsBrucella Vaccine
Owner:CHINA INST OF VETERINARY DRUG CONTROL
New-generation brucellosis antibody competition enzyme-linked immunosorbent adsorption test detection kit
ActiveCN101581726AAccurate diagnosisMeet the needs of epidemic preventionMaterial analysisFc(alpha) receptorAntigen
The invention aims to provide a new-generation brucellosis antibody competition enzyme-linked immunosorbent adsorption test detection kit which meets animal epidemic prevention requirements in China, is sensitive and specific and can accurately diagnose brucellosis. In the invention, the lipopolysaccharide of smooth type brucella is used as an antigen-coated enzyme-labeled plate, inactivated bacilli liquid is used for immunizing or artificially infecting a healthy cattle to prepare positive control sera and standard weak positive sera, healthy non-immune cattle sera are used as negative control sera, a novel monoclonal antibody is used as a competition antibody, the protein AG enzyme-labeled substance of an anti-Fc receptor is used as an assistant antibody, serum diluent, a washing solution, a substrate solution A, a substrate solution B, H2O2 and a stop solution are prepared, and all raw materials are assembled to form the kit. The invention is a competition ELISA kit which is sensitive and specific and can accurately diagnose brucellosis. The invention meets animal epidemic prevention requirements in China, and carries out last diagnosis on the brucellosis by means of a laboratory diagnosis technique.
Owner:哈尔滨平河生物技术有限公司 +2
Immunogenic compositions including rough phenotype Brucella host strains and complementation DNA fragments
InactiveUS20050142151A1Sufficient attenuationEasy to operateBiocideBacterial antigen ingredientsHeterologousHeterologous Antigens
Live attenuated vaccines against brucellosis and infection by other diseases are described. It has been discovered that trans complementation of the Brucella wboA gene can be used to maintain an expression vector in an attenuated Brucella host cell in a vaccinee. Further, heterologous antigens can be expressed using this Brucella platform, thus effecting a multivalent vaccine against Brucella and the disease corresponding to the heterologous antigen.
Owner:UNITED STATES OF AMERICA THE AS REPRESENTED BY THE SEC OF THE ARMY
Detection kit and detection method for brucellae in meat products
InactiveCN101570780ARapid identificationStrong specificityComponent separationMicrobiological testing/measurementBiologyPcr dgge
The invention discloses a detection kit and a detection method for brucellae in meat products. The kit comprises Taq DNA polymerase with a concentration of 5U / mu L and a PCR reaction solution, wherein the PCR reaction solution contains 10 millimols of Tris.HCl, 50 millimols of KCl, 25 millimols of MgCl2, 2.5 millimols of dNTP and 0.2 millimol of general brucella detection primer. The kit and the detection method of the invention can detect 6 species of brucellae of genus brucella and detect the 6 species of brucellae respectively. The kit has high specificity and sensitivity and can detect brucellae with a concentration of 19 CFU / mL. The detection method has the advantages of quick, simple and convenient operation and low detection cost.
Owner:曹际娟 +2
RPA-LFA (recombinase polymerase amplification-lateral flow assay) detection kit for detecting brucella and use method of kit
InactiveCN109337994ASuitable for the siteSuitability for useMicrobiological testing/measurementMicroorganism based processesPositive controlAssay
The invention discloses an RPA-LFA (recombinase polymerase amplification-lateral flow assay) detection kit for detecting brucella. The kit comprises (1) a sample tretament solution, (2) a detection reaction solution, (3) a dry powder tube, (4) a lateral flow test strip, (5) a positive control reaction solution, (6) a negative control reaction solution and (7) a sample treatment solution. The kit combines simple treatment of samples, RPA and LFA technologies, no simultaneous equipment is required, and all that is required for detection is to boil the samples, so that the kit is very suitable tobe used on site or in fields, can detect and diagnose brucella in veterinarian clinics or farms, has great clinical application value for diagnosis and control of the diseases and has high detectionprecision and broad market prospects.
Owner:SHENYANG AGRI UNIV
Brucella melilitensis bp26 gene-deleted M5-90 vaccine strain
The invention relates to a recombined brucella melilitensis, wherein the recombined brucella melilitensis completely deletes the bp26 gene. Safe tests prove that the brucella melilitensis is consistent with a parent vaccine strain. Virulent attack protection tests prove that a mutant strain has immune protection consistent with the parent vaccine strain. The mutant strain has significance for distinguishing vaccine immunity from wild type brucellosis infection from the standpoint of serology and controlling, monitoring and purifying brucellosis disease.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Detection method of brucella, kit and applications of kit
InactiveCN106191286AFull True Reflection ExtractionComprehensive and true reflection of amplificationMicrobiological testing/measurementMicroorganism based processesEscherichia coliSerum samples
The invention discloses a detection method of brucella. The detection method comprises the following steps: acquiring brucella DNA from a serum sample by adopting a centrifugal column method, thus obtaining a to-be-detected sample; carrying out PCR amplification reaction; detecting a reaction result by adopting a fluorescence quantitative PCR instrument, wherein during fluorescence signal collection, fluorescein corresponding to fluorophore at the BKV-FP 5' end of a Taqman fluorescence probe is set, and the fluorescence signals are collected at 60 DEG C; and analyzing the result. The invention further provides a kit for detecting the brucella. The detection method and the kit are high in sensitivity, and the minimal detection can achieve 1000 copies / mL. Meanwhile, the detection method is good in specificity, and the cross reaction with other bacteria, such as escherichia coli, salmonella enteritidis, hepatitis B virus, human herpes virus 4, and human cytomegalovirus can be avoided.
Owner:北京旌准医疗科技有限公司
Animal brucella antibody colloidal gold test paper film detection reagent kit
The invention discloses an animal brucella antibody colloidal gold test paper film detection kit, which bases on immunology basic principle of antigen and antibody specific combination, utilizes colloidal gold immunity chromatography technique and silver dye reinforcement technique, uses combined golden yellow staphylococcus A protein (SPA) as gold marking antibody, uses high purity brucella LPS as peridium antigen, and utilizes goat anti-bovine IgG as quality control line to prepare colloidal gold test paper tape. The invention can accurately differentiate species of animal brucella and can synchronously detect animal brucella disease of cattle, sheep and pig species with no crossing reaction and low false negative proportion, negative and positive coincidence ratio of the invention and conventional separation and culture is 100%, detection result can be obtained in 5 min, and the kit need no assistant complicated detection instrument.
Owner:MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Development of a live, attenuated, recombinant vaccine for Brucellosis
InactiveUS20070036823A1Treating and preventing BrucellosisBacterial antigen ingredientsBacteriaProteinase activityRecombinant vaccines
A recombinant, attenuated strain of Brucella suis with a deficiency in carboxyl-terminal protease (CtpA) activity can be used as a vaccine for the prevention or treatment of Brucellosis. Prior exposure to the Brucella species is identified by detecting a genetic sequence for carboxyl-terminal protease activity in a biological sample.
Owner:VIRGINIA TECH INTPROP INC
Development of a live, attenuated, recombinant vaccine for Brucellosis
A recombinant, attenuated strain of Brucella suis or Brucella melitensis with a deficiency in carboxyl-terminal protease activity or tail-specific protease activity can be used as a vaccine for the prevention or treatment of Brucellosis. Prior exposure to the Brucella species is identified by detecting a genetic sequence for carboxyl-terminal (i.e. tail-specific) protease activity in a biological sample.
Owner:VIRGINIA TECH INTPROP INC
Preparation method for Brucella shell vaccine strain
InactiveCN103952428AGood genetic stabilityImprove securityBacteriaMicroorganism based processesTemperature controlLysis
A preparation method for Brucella shell vaccine strain is disclosed, relates to the field of molecular biology and microbiology, and helps to solve the problem that conventional bacteria shell preparation method is high in cost and not suitable for large-scale production. The method comprises: respectively cloning PCR products of homogenous arms at the upstream and at the downstream of a specific gene of Brucella strain genome to a pMD18-T Simple vector to construct recombinant plasmids, performing double digestion on the recombinant plasmids, successively connecting with plasmid pBK-CMV-SacB subjected to digestion processing for constructing a suicide plasmid, performing PCR amplification on a temperature-control lysis part of a temperature-control expression plasmid pBV220-E, performing digestion processing on the amplification product, inserting into the suicide plasmid; and transforming the temperature-control lysis type suicide plasmid into competent Brucella, and performing positive and negative screening by utilizing kanamycins resistance and fructose sucrase gene, so as to obtain the Brucella shell vaccine strain. The Brucella shell vaccine strain has good heredity stability, security, effectiveness and immunogenicity.
Owner:MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Method and kit for detecting multiple high-pathogenicity pathogenic bacteria
ActiveCN102796810AImprove throughputAddresses issues with reduced sensitivityMicrobiological testing/measurementAgainst vector-borne diseasesBacteroidesHighly pathogenic
The invention provides a method and kit for detecting multiple high-pathogenicity pathogenic bacteria, particularly a method for simultaneously detecting multiple high-pathogenicity pathogenic bacteria, which comprises the following steps: in a polymerase reaction system, carrying out PCR (polymerase chain reaction) on high-pathogenicity pathogenic bacteria by using a specific primer set, thereby obtaining an amplification product; and detecting with a specific probe or probe microsphere. The invention also provides a corresponding kit. The invention can sensitively and conveniently detect and identify multiple high-pathogenicity pathogenic bacteria, including Bacillus anthracis, Yersinia pestis, Clostridium botulinum, Brucella, Streptococcus suis, Vibrio cholerae, Francisella tularensis, Pseudomonas mallei (or Pseudomonas pseudomallei), Coxiella burnetii and Legionella pneumophila.
Owner:SHANGHAI TELLGEN LIFE SCI CO LTD
Brucella Omp10 protein antigen epitope polypeptide and application thereof
ActiveCN105693833AImprove the level of prevention and control technologyPromote healthy developmentAntibacterial agentsBacterial antigen ingredientsHigh concentrationChemical synthesis
The invention discloses Brucella Omp10 protein antigen epitope polypeptide and application thereof. The polypeptide's amino acid sequence is SEQ ID No.1 or the polypeptide is formed by the sequence formed by SEQ ID No.1 with one or several amino acid residues replaced and / or lacked or could be derived from the amino acid sequence SEQ ID No.1 added with Brucella immunogenicity function. The polypeptide with SEQ ID No.1 can generate recombinant carriers or expression cassettes or transgenosis cells or recombinant bacteria. The invention further relates to application of the polypeptide in preparing reagent or medicine for diagnosing or preventing or treating diseases caused by Brucella. The polypeptide is chemically synthesized, can release high-concentration IL-4 under DC stimulation, can detect serum infected by brucellosis as target antigen, or prepare antigen of Omp 25 protein monoclonal antibody.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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