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Low virulent strain of Brucella and vaccine thereof

A technology of Brucella and Brucella bovis, applied in the direction of bacteria, antibacterial drugs, bacterial antigen components, etc.

Active Publication Date: 2014-08-13
CHINA INST OF VETERINARY DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no recombinant Brucella attenuated strains and vaccines obtained by genetic modification at home and abroad to be approved and applied.

Method used

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  • Low virulent strain of Brucella and vaccine thereof
  • Low virulent strain of Brucella and vaccine thereof
  • Low virulent strain of Brucella and vaccine thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Pancreatic Soy Broth (TSB, BD Company, USA) or other suitable culture medium is used as the culture medium for the rough Brucella vaccine strain (RA343). Add 1% to 2% of the amount to the RA343 seed bacterial liquid, gradually increase the ventilation at 36 to 37°C, and ferment and cultivate for 36 hours according to the conventional method. 1% to 2% of the total medium.

[0059] After the cultivation is completed, the samples are purely tested with pancreatic soybean agar according to the method stipulated in "Chinese Veterinary Pharmacopoeia". At the same time, take samples and use TSA medium to count the viable bacteria, which can be used as a reference when concentrating.

[0060] Add 0.1% to 0.2% of the total amount of sodium carboxymethyl cellulose to the bacteria liquid that has passed the pure inspection to precipitate the bacteria, absorb the supernatant, store it temporarily at 2 to 8°C, and wait for packaging.

[0061] Take the concentrated bacterial soluti...

Embodiment 2

[0065] Finished product inspection, wherein the vaccine is prepared according to the technical scheme provided by the present invention.

[0066] The relevant inspection methods involved in the present invention are carried out according to the method of "Chinese Veterinary Pharmacopoeia"

[0067] (1) Physical properties The vaccine should be loose and fast, and can dissolve rapidly within 1 minute after adding the diluent;

[0068] (2) The purely tested vaccines are purely free of other bacteria;

[0069] (3) Safety inspection Dilute the vaccine to contain 1×10 per 1ml 9 CFU live bacteria, subcutaneously inoculated 5 Kunming mice of 18-20 g in the groin, each 0.25 ml / only, observed for 6 days, and all of them were healthy and alive (results are shown in Table 2);

[0070] Table 2 brucellosis live vaccine (RA343 strain) safety inspection results

[0071]

[0072] -: Healthy survival, no abnormality at the injection site; +: Death or morbidity.

[0073] (4) Counting of v...

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Abstract

The invention relates to a low virulent strain of Brucella and a vaccine thereof. According to a vaccine strain, a coarse low virulent strain RA343 of Brucella abortus is screened through a domestication and selection technology combining antibiotics and A-factor serum. The coarse low virulent strain is remarkably improved in safety and still remains a good immune effect on the Brucella is still maintained. A Brucella vaccine prepared by using the low virulent strain is to change the current situation that Brucella vaccine-immunized animals and wild strain-infected animals are hard to differentiate, and the safety of existing vaccine is effectively improved.

Description

[0001] Technical field The present invention relates to an attenuated strain of Brucella and its vaccine. The vaccine is a live vaccine for preventing brucellosis of cattle, sheep and pigs, and belongs to the field of veterinary biological products. technical background [0002] Brucellosis (brucellosis) is a zoonotic disease characterized by abortion and fever caused by Brucella or Brucella, which seriously threatens the life and health of humans and various animals. . This disease not only has serious harm to the reproduction and production performance of animals, but more importantly, people are often difficult to cure after being infected with Brucella, thus causing serious public health problems. Elimination of brucellosis has therefore been one of the most important goals of public health programs in countries where Brucella is endemic. At present, the main method to eliminate the disease worldwide is the combination of culling and immunization. For China, where the oc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/10A61P31/04C12R1/01
Inventor 丁家波朱良全王芳冯忠武蒋卉毛开荣陈光华王楠宁宜宝李慧娇王忠田张广川程君生
Owner CHINA INST OF VETERINARY DRUG CONTROL
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