Brucella rapid detection kit based on mass-spectrometric technique

A Brucella and kit technology, applied in the field of protein fingerprint detection, can solve problems such as the complex composition of bacterial blood samples, achieve the effect of saving detection time and cost, and eliminating hazards to personnel and the environment
CN103308696AInactive Publication Date: 2013-09-18ICDC CHINA CDC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ICDC CHINA CDC
Publication Date
2013-09-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a kit for rapidly detecting brucella in a blood sample, relating to the technical field of fingerprint spectrum detection of proteins. According to the kit, based on the mass-spectrometric technique, a specific polypeptide mass-spectrometric model aiming at brucella detection in the blood is constructed, and a kit for carrying out the brucella detection by utilizing the mass-spectrometric technique is constructed. According to the kit provided by the invention, the brucella in the blood sample can be authenticated by utilizing a specific fingerprint spectrum, the accuracy is high, and the repeatability is good; the detection on 96 samples can be finished within 2 hours and can be finished at least 5 days in advance compared with the detection time in the conventional detection method; the detection cost is low, and the result is reliable, so that the kit has a good application prospect.
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Description

technical field

[0001] The invention relates to the technical field of protein fingerprint detection, in particular to a mass spectrometry model for detecting Brucella characteristic proteins and a kit for detecting Brucella based on the mass spectrometry model. Background technique

[0002] Brucella is a gram-negative intracellular parasite with no spores and no flagella and is pathogenic to humans and animals. It is the pathogen of brucellosis (brucellosis). As a serious zoonotic infectious disease, brucellosis has become one of the important public health problems in our country. The epidemic situation among humans and animals has risen sharply in the past 10 years, and the epidemic situation has shown a trend of spreading from pastoral and semi-pastoral areas to agricultural areas and even cities. The number of new cases is about 40,000 every year, and the prevention and control situation is very severe. The types of Brucella include sheep (3 types), cattle (8 types), p...

Claims

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