10 results about "Specific lysis" patented technology

The invention relates to the technical field of bacteriophages, and particularly discloses an acinetobacter baumannii bacteriophage, depolymerases and a preparation method and application of the acinetobacter baumannii bacteriophage. The acinetobacter baumannii bacteriophage P919 provided by the invention has very strong specific lysis capability on KL3 type acinetobacter baumannii, and the coded depolymerases of the acinetobacter baumannii bacteriophage P919 have excellent temperature stability at 70 DEG C or below, and can effectively degrade capsular polysaccharide of the KL3 type acinetobacter baumannii.

The invention discloses an enterococcus faecalis bacteriophage vBEfaPFA2, a bacteriophage composition and application of the enterococcus faecalis bacteriophage vBEfaPFA2, the enterococcus faecalis bacteriophage vBEfaPFA2 has a specific lysis effect on enterococcus faecalis and also has a cross-species lysis effect on salmonella, the acid-base adaptability of the bacteriophage is high, and the bacteriophage vBEfaPFA2 and the bacteriophage composition can be used for preparing the enterococcus faecalis bacteriophage The enterococcus faecalis bacteriophage can be used as an active component to be prepared into the bacteriophage composition or the bacteriophage pharmaceutical preparation, the bacteriophage composition or the bacteriophage pharmaceutical preparation is applied to preparation of a medicine or a bacteriostatic agent for preventing and treating enterococcus faecalis and salmonella infection, the enterococcus faecalis bacteriophage and the bacteriophage composition are safe to use, high in yield and easy to industrially produce, and due to the cross-species lysis performance, the bacteriophage can be The method has the advantages of wide splitting spectrum, wide application range and good application prospect.

The application discloses a method for eliminating passivation layer in bioleaching, a phage and application thereof, and belongs to the technical field of biohydrometallurgy. Mainly, the technical problem of passivation layer easily generated in the existing ore bioleaching process and low leaching efficiency is solved. The method utilizes the specific lysis of phage to the aging or overproliferation of bacteria adsorbed on the ore surface, destroys the passivation film structure composed of biofilm and sulfur-containing precipitate, exposes the fresh mineral surface, and thus significantly restores and improves the leaching efficiency of copper ions. The application has wide application prospect.

The invention discloses an enterococcus faecalis bacteriophage vBEfaPFA3, a bacteriophage composition and application of the enterococcus faecalis bacteriophage vBEfaPFA3, the enterococcus faecalis bacteriophage vBEfaPFA3 has a specific lysis effect on enterococcus faecalis and also has a cross-species lysis effect on salmonella, the acid-base adaptability of the bacteriophage is high, and the enterococcus faecalis bacteriophage vBEfaPFA3 can be used as an active component to be prepared into the bacteriophage composition or a bacteriophage pharmaceutical preparation. The enterococcus faecalis bacteriophage and the bacteriophage composition are applied to preparation of drugs or bacteriostatic agents for preventing and treating enterococcus faecalis and salmonella infection, are safe to use, have no side effects, are easy to industrially produce, and are wide in lysis spectrum and wide in application range based on the cross-species lysis performance of the enterococcus faecalis bacteriophage and the bacteriophage composition.

The application discloses a broad-spectrum bacteriophage targeting mucoid Escherichia coli, Cronobacter and Salmonella and application thereof Escherichia coli phage vB EcoP 3BB7B, the bacteriophage can specifically lyse colanic acid-producing mucoid Escherichia coli, and breaks through the technical bottleneck that a mucous layer limits adsorption of most bacteriophages; further has cross-species lysis capacity, not only has good lysis activity on mucoid Escherichia coli, but also can effectively lyse important foodborne pathogenic bacteria such as Salmonella of various serotypes and Cronobacter sakazakii, and significantly widens the host spectrum range. The bacteriophage can realize efficient amplification under the condition of a lower infection multiple, has good environmental stability, has strong lysis activity, does not carry drug resistance genes and virulence-related genes, has high safety, and has a good application prospect in clinical and food safety prevention and control.

The present application relates to the technical field of bacteriophage, and discloses a bacteriophage of Pseudomonas syringae pv. actidii and application thereof, and the preservation number of the bacteriophage is CGMCC No. 45227. The bacteriophage provided by the present application can specifically lyse Pseudomonas syringae pv. actidii, and therefore, the bacteriophage can be used for preventing and treating bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial bacterial

This invention provides a method for isolating cell nuclei from *Pinus tabuliformis* tissue, relating to the field of plant molecular and cell biology. The method uses *Pinus tabuliformis* callus tissue as explants, releases protoplasts using a specific enzymatic hydrolysis solution, lyses them using a specific lysis buffer, and finally filters and centrifuges to obtain *Pinus tabuliformis* cell nuclei. Using this invention, cell nuclei suitable for single-cell and histone modification library construction and sequencing can be rapidly isolated, providing a foundation for research on single-cell nuclear genetic material transcription analysis, tissue-specific analysis, and cell fate determination in gymnosperms, and offering research ideas for the future use of cell nuclei in coniferous genetic engineering.

This invention relates to the field of phage technology, specifically disclosing an Acinetobacter baumannii phage, a depolymerase, its preparation method, and its applications. The Acinetobacter baumannii phage P919 provided by this invention exhibits strong specific lytic ability against Acinetobacter baumannii type KL3, and its encoded depolymerase possesses excellent temperature stability below 70°C, effectively degrading the capsular polysaccharide of Acinetobacter baumannii type KL3.

The invention discloses an enterococcus faecalis bacteriophage vBEfaPFA1, a bacteriophage composition and application of the enterococcus faecalis bacteriophage vBEfaPFA1, the enterococcus faecalis bacteriophage vBEfaPFA1 has a specific lysis effect on enterococcus faecalis and also has a cross-species lysis effect on salmonella, the acid-base adaptability of the bacteriophage is high, and the enterococcus faecalis bacteriophage vBEfaPFA1 can be used as an active component to be prepared into the bacteriophage composition or a bacteriophage pharmaceutical preparation. The enterococcus faecalis bacteriophage and the bacteriophage composition are applied to preparation of drugs or bacteriostatic agents for preventing and treating enterococcus faecalis and salmonella infection, are safe to use, have no side effects, are easy to industrially produce, and are wide in lysis spectrum and wide in application range based on the cross-species lysis performance of the enterococcus faecalis bacteriophage and the bacteriophage composition.

The invention relates to the technical field of microorganisms, and provides a bacteriophage perforin HolKpV318 with a broad-spectrum antibacterial effect and application of the bacteriophage perforin HolKpV318. The invention provides a potential novel antibacterial strategy aiming at the clinical problem of increasingly severe multi-drug-resistant klebsiella pneumoniae infection caused by antibiotic abuse at present, so as to overcome the defect that the traditional antibiotic treatment is invalid or poor in effect; the invention provides the perforin protein (HolKpV318) with efficient and specific klebsiella pneumoniae splitting capability, and the protein can form a pore channel on a klebsiella pneumoniae cell membrane to destroy the integrity of a bacterial structure so as to cause the death of target bacteria, so that a brand new biological preparation choice is provided for solving the problem of drug-resistant bacterium infection.